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The map label for this gene is aspA [H]

Identifier: 146301145

GI number: 146301145

Start: 4066772

End: 4068181

Strand: Reverse

Name: aspA [H]

Synonym: Fjoh_3403

Alternate gene names: 146301145

Gene position: 4068181-4066772 (Counterclockwise)

Preceding gene: 146301147

Following gene: 146301144

Centisome position: 66.73

GC content: 35.32

Gene sequence:

>1410_bases
ATGGAACCAACAAGAAAGGAACATGATTTTTTAGGAGAATTAGAAATTCCAAATCATTTATACTACGGAATCCAAACTTT
TAGAGCGGTAGAAAATTTTAATATTACAGGAATTCCTATTTCAAAAGAGCCTTTATTTATCAAAGCTTTAGGATATGTAA
AAAAAGCGGCAGCTTTAGCAAATAAAGACTGCGGCGCAATCGATTCGAAACTTGCAGAAGCCATTTGTTATGGAAGCGAC
CAGGTAATTTCGGGTAAATTTGATAAGGAATTTGTCAGTGATTTAATTCAGGGCGGAGCAGGAACTTCTGTAAACATGAA
TGCTAATGAAGTAATTGCCAATATCGGACTGGAATATTTAGGTCATAAAAAAGGCGAATATAATTTTCTGCATCCCAATA
ATCATGTAAACTGTTCGCAGTCTACAAATGATGCTTATCCATCGGCATTTAGAATTGCTTTATATCTTAAAATGGAAAGC
TTCATTAAAACTTTAGCTGGTTTAGAAGTTGCTTTCGCTAAAAAAGGAGAGGAGTTTAAAGAAGTCCTGAAAATGGGAAG
AACGCAGCTTCAGGACGCGGTGCCAATGACTTTAGGGCAAGAATTTAAAGCCTATGCGACAACAATTGGCGAAGATATTC
AGCGTTTAAAAAATGCTCAGGAATTATTGTTGGAAATCAATATGGGAGCAACAGCCATTGGAACAAAAGTAAATGCTCCG
GAAGGTTATCCGGAAATTTGTGTTTCTTATTTAGCCAAAGAAGTTGGAATTCCGTTAACACTATCACCAGATTTAATTGA
AGCAACAGTTGATACAGGCGCTTATGTGCAGATTATGGGAACTTTAAAACGTTCTGCGGTTAAAATATCTAAAATCTGCA
ACGATTTACGATTACTAAGTTCAGGCCCAAGAACAGGTTTTAATGAAATCAATCTTCCGGCACGCCAGCCGGGATCGTCT
ATTATGCCTGGAAAAGTAAATCCGGTAATTCCAGAAGTAGTAAACCAGACTTGTTTTTATGTAATAGGTCAGGATTTAAC
AGTTACAATGGCAGCAGAAGCAGGACAATTGCAATTGAATGTAATGGAACCTGTAATTGCTTTTGCAATGTTTACTTCGC
TGGATTATCTTTCAAATGCTATTCAGACTTTAATCGATAAATGTATTAACGGAATCACCGCAAATGTAGATCACTGCTAC
AATATGGTTATGAACAGTATTGGAATTGTAACACAGTTAAACCCAATTATTGGTTATGAAGAAAGCGCCAGTATCGCAGG
TGAAGCTTTAAAAACAAACAAAAGTGTTCATCAAATAGCTGTTGTAGAAAGAAAACTTATTACACAGGAAAAATGGGATG
AAATTTATTCTTTAGAAAATTTAATTCATCCAAAATTTATAACAAAATAA

Upstream 100 bases:

>100_bases
CTTAAAATGCAAATCATTTTCATTTCATTCACATTTTTAATCCACAAAATGCTGTAGCTTTACACTTATTTTTTAAGTTA
AAACAGATCAATTTTATTAT

Downstream 100 bases:

>100_bases
TATTGTTATAAAAAATATAATAACAAGCCGTCGGTAAATCGACGGCTTTTTTTGTTTTATAGGTAAAAGTTATTGTGTAA
AGTCTTGAAAATGAATTATA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MEPTRKEHDFLGELEIPNHLYYGIQTFRAVENFNITGIPISKEPLFIKALGYVKKAAALANKDCGAIDSKLAEAICYGSD
QVISGKFDKEFVSDLIQGGAGTSVNMNANEVIANIGLEYLGHKKGEYNFLHPNNHVNCSQSTNDAYPSAFRIALYLKMES
FIKTLAGLEVAFAKKGEEFKEVLKMGRTQLQDAVPMTLGQEFKAYATTIGEDIQRLKNAQELLLEINMGATAIGTKVNAP
EGYPEICVSYLAKEVGIPLTLSPDLIEATVDTGAYVQIMGTLKRSAVKISKICNDLRLLSSGPRTGFNEINLPARQPGSS
IMPGKVNPVIPEVVNQTCFYVIGQDLTVTMAAEAGQLQLNVMEPVIAFAMFTSLDYLSNAIQTLIDKCINGITANVDHCY
NMVMNSIGIVTQLNPIIGYEESASIAGEALKTNKSVHQIAVVERKLITQEKWDEIYSLENLIHPKFITK

Sequences:

>Translated_469_residues
MEPTRKEHDFLGELEIPNHLYYGIQTFRAVENFNITGIPISKEPLFIKALGYVKKAAALANKDCGAIDSKLAEAICYGSD
QVISGKFDKEFVSDLIQGGAGTSVNMNANEVIANIGLEYLGHKKGEYNFLHPNNHVNCSQSTNDAYPSAFRIALYLKMES
FIKTLAGLEVAFAKKGEEFKEVLKMGRTQLQDAVPMTLGQEFKAYATTIGEDIQRLKNAQELLLEINMGATAIGTKVNAP
EGYPEICVSYLAKEVGIPLTLSPDLIEATVDTGAYVQIMGTLKRSAVKISKICNDLRLLSSGPRTGFNEINLPARQPGSS
IMPGKVNPVIPEVVNQTCFYVIGQDLTVTMAAEAGQLQLNVMEPVIAFAMFTSLDYLSNAIQTLIDKCINGITANVDHCY
NMVMNSIGIVTQLNPIIGYEESASIAGEALKTNKSVHQIAVVERKLITQEKWDEIYSLENLIHPKFITK
>Mature_469_residues
MEPTRKEHDFLGELEIPNHLYYGIQTFRAVENFNITGIPISKEPLFIKALGYVKKAAALANKDCGAIDSKLAEAICYGSD
QVISGKFDKEFVSDLIQGGAGTSVNMNANEVIANIGLEYLGHKKGEYNFLHPNNHVNCSQSTNDAYPSAFRIALYLKMES
FIKTLAGLEVAFAKKGEEFKEVLKMGRTQLQDAVPMTLGQEFKAYATTIGEDIQRLKNAQELLLEINMGATAIGTKVNAP
EGYPEICVSYLAKEVGIPLTLSPDLIEATVDTGAYVQIMGTLKRSAVKISKICNDLRLLSSGPRTGFNEINLPARQPGSS
IMPGKVNPVIPEVVNQTCFYVIGQDLTVTMAAEAGQLQLNVMEPVIAFAMFTSLDYLSNAIQTLIDKCINGITANVDHCY
NMVMNSIGIVTQLNPIIGYEESASIAGEALKTNKSVHQIAVVERKLITQEKWDEIYSLENLIHPKFITK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=39.1774891774892, Blast_Score=328, Evalue=6e-90,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=56.8669527896996, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1787896, Length=434, Percent_Identity=36.8663594470046, Blast_Score=294, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17553882, Length=467, Percent_Identity=41.3276231263383, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=38.4146341463415, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=38.8768898488121, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=39.9568034557235, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=39.9568034557235, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=39.2241379310345, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=37.9310344827586, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24583245, Length=465, Percent_Identity=37.2043010752688, Blast_Score=302, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51401; Mature: 51401

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPTRKEHDFLGELEIPNHLYYGIQTFRAVENFNITGIPISKEPLFIKALGYVKKAAALA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHH
NKDCGAIDSKLAEAICYGSDQVISGKFDKEFVSDLIQGGAGTSVNMNANEVIANIGLEYL
CCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHCHHHH
GHKKGEYNFLHPNNHVNCSQSTNDAYPSAFRIALYLKMESFIKTLAGLEVAFAKKGEEFK
CCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHCCHHHH
EVLKMGRTQLQDAVPMTLGQEFKAYATTIGEDIQRLKNAQELLLEINMGATAIGTKVNAP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCC
EGYPEICVSYLAKEVGIPLTLSPDLIEATVDTGAYVQIMGTLKRSAVKISKICNDLRLLS
CCCHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGPRTGFNEINLPARQPGSSIMPGKVNPVIPEVVNQTCFYVIGQDLTVTMAAEAGQLQLN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEEEEEECCCCCEEEH
VMEPVIAFAMFTSLDYLSNAIQTLIDKCINGITANVDHCYNMVMNSIGIVTQLNPIIGYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHCCCHHCCCC
ESASIAGEALKTNKSVHQIAVVERKLITQEKWDEIYSLENLIHPKFITK
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MEPTRKEHDFLGELEIPNHLYYGIQTFRAVENFNITGIPISKEPLFIKALGYVKKAAALA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHH
NKDCGAIDSKLAEAICYGSDQVISGKFDKEFVSDLIQGGAGTSVNMNANEVIANIGLEYL
CCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHCHHHH
GHKKGEYNFLHPNNHVNCSQSTNDAYPSAFRIALYLKMESFIKTLAGLEVAFAKKGEEFK
CCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHCCHHHH
EVLKMGRTQLQDAVPMTLGQEFKAYATTIGEDIQRLKNAQELLLEINMGATAIGTKVNAP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCC
EGYPEICVSYLAKEVGIPLTLSPDLIEATVDTGAYVQIMGTLKRSAVKISKICNDLRLLS
CCCHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGPRTGFNEINLPARQPGSSIMPGKVNPVIPEVVNQTCFYVIGQDLTVTMAAEAGQLQLN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCEEEEEECCCCCEEEH
VMEPVIAFAMFTSLDYLSNAIQTLIDKCINGITANVDHCYNMVMNSIGIVTQLNPIIGYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHCCCHHCCCC
ESASIAGEALKTNKSVHQIAVVERKLITQEKWDEIYSLENLIHPKFITK
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]