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The map label for this gene is leuA [H]

Identifier: 146300614

GI number: 146300614

Start: 3400160

End: 3401341

Strand: Reverse

Name: leuA [H]

Synonym: Fjoh_2865

Alternate gene names: 146300614

Gene position: 3401341-3400160 (Counterclockwise)

Preceding gene: 146300616

Following gene: 146300613

Centisome position: 55.79

GC content: 36.89

Gene sequence:

>1182_bases
ATGAATATGAATAGAGAGAAAGTTCAAATTTTTGACACCACTTTACGCGACGGTGAGCAAGTTCCAGGATGCAAGTTAGA
TACTAAGCAAAAATTAGTTATCGCAGAACGACTAGACAAAATGGGAGTTGATATTATCGAAGCTGGTTTTCCTGTGTCAA
GTCCGGGCGATTTTTTATCGGTCTCTGAGATTTGTAAAATTGTAGAAAATGCAACGGTCTGCGGACTAACAAGAGCCGTG
AAAAACGACATTGATGTTGCTGCAGCTGCTTTAAAGCACGCGAAGAAACCTAGAATCCATACTGGAATCGGAACTTCTGA
ATCTCATATACTCCACAAATTACAAACTACGCCTGAAGATATTATTGCAAGAGCCAAATTTGCAGTAGCACACGCAAAAT
CGTATGTAGAAGATGTAGAATTCTACGCAGAAGATGCTGGTAGAACTGATAATGCTTTCCTTGCAAAAGTTTGTGAAGAA
GTTATTAAATCTGGTGCAACTGTATTAAATATTCCTGACACAACAGGATATTGTCTTCCGGAAGAATATGGAGCAAAGAT
TAAATATTTAAAAGAAAACGTAAAAGGAATCGAAAACGTAATCCTTTCATGCCACTGTCATAACGATTTAGGAATGGCAA
CTGCAAACTCAATTGCAGGTGCAATTAATGGTGCAAGACAAATCGAGTGTACTATTAACGGTATTGGAGAAAGAGCAGGA
AATACTGCACTTGAAGAAGTGGTAATGATTTTTAAACAACATCCTTACTTAAACTTAGATACAAACATCAATACAAGAGA
ATTAAACGAAATGAGCCGTTTAGTTTCTGAAAGTATGGGAATGATTGTACAGCCAAATAAAGCTATCGTTGGAGCAAATG
CTTTTGCACATAGTTCTGGAATTCATCAGGATGGTGTTATCAAAAACAGAGCAACTTACGAAATTATGGATCCGCTTGAT
GTGGGTGTAAACGAATCTTCTATCATTTTGACTGCAAGAAGCGGAAGAGCAGCATTAGCGTACCGTGCTAAAAAAGTAGG
TTACGAGCTTACAAAAACACAATTAGACACTGTTTACATTGAGTTCTTGAAGTTTGCTGACATTAAAAAAGAAGTAGTAG
ACGCAGATATTCACCAAATTATAGAAGCTTCTAAAATTGAAGGTGAATTAATCAGAAGCTAA

Upstream 100 bases:

>100_bases
ATAAATTGTGTTATGAACAGTATAATTTCATTAAAATGAATTTCGTACTGCGATTGTACAATATATATTTGTTATCAAGA
TGTGAAATTACATTAAATAA

Downstream 100 bases:

>100_bases
TAAAACACAATAGAGCCAAGAAGAAAGAGAAAAAAATAAAGATTTAAATACATAAAGAACGAGACGTTATGAATTTGAAA
ATAGCAGTTTTACCAGGAGA

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNMNREKVQIFDTTLRDGEQVPGCKLDTKQKLVIAERLDKMGVDIIEAGFPVSSPGDFLSVSEICKIVENATVCGLTRAV
KNDIDVAAAALKHAKKPRIHTGIGTSESHILHKLQTTPEDIIARAKFAVAHAKSYVEDVEFYAEDAGRTDNAFLAKVCEE
VIKSGATVLNIPDTTGYCLPEEYGAKIKYLKENVKGIENVILSCHCHNDLGMATANSIAGAINGARQIECTINGIGERAG
NTALEEVVMIFKQHPYLNLDTNINTRELNEMSRLVSESMGMIVQPNKAIVGANAFAHSSGIHQDGVIKNRATYEIMDPLD
VGVNESSIILTARSGRAALAYRAKKVGYELTKTQLDTVYIEFLKFADIKKEVVDADIHQIIEASKIEGELIRS

Sequences:

>Translated_393_residues
MNMNREKVQIFDTTLRDGEQVPGCKLDTKQKLVIAERLDKMGVDIIEAGFPVSSPGDFLSVSEICKIVENATVCGLTRAV
KNDIDVAAAALKHAKKPRIHTGIGTSESHILHKLQTTPEDIIARAKFAVAHAKSYVEDVEFYAEDAGRTDNAFLAKVCEE
VIKSGATVLNIPDTTGYCLPEEYGAKIKYLKENVKGIENVILSCHCHNDLGMATANSIAGAINGARQIECTINGIGERAG
NTALEEVVMIFKQHPYLNLDTNINTRELNEMSRLVSESMGMIVQPNKAIVGANAFAHSSGIHQDGVIKNRATYEIMDPLD
VGVNESSIILTARSGRAALAYRAKKVGYELTKTQLDTVYIEFLKFADIKKEVVDADIHQIIEASKIEGELIRS
>Mature_393_residues
MNMNREKVQIFDTTLRDGEQVPGCKLDTKQKLVIAERLDKMGVDIIEAGFPVSSPGDFLSVSEICKIVENATVCGLTRAV
KNDIDVAAAALKHAKKPRIHTGIGTSESHILHKLQTTPEDIIARAKFAVAHAKSYVEDVEFYAEDAGRTDNAFLAKVCEE
VIKSGATVLNIPDTTGYCLPEEYGAKIKYLKENVKGIENVILSCHCHNDLGMATANSIAGAINGARQIECTINGIGERAG
NTALEEVVMIFKQHPYLNLDTNINTRELNEMSRLVSESMGMIVQPNKAIVGANAFAHSSGIHQDGVIKNRATYEIMDPLD
VGVNESSIILTARSGRAALAYRAKKVGYELTKTQLDTVYIEFLKFADIKKEVVDADIHQIIEASKIEGELIRS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=373, Percent_Identity=53.0831099195711, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6320019, Length=391, Percent_Identity=30.690537084399, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6320071, Length=384, Percent_Identity=30.7291666666667, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324682, Length=367, Percent_Identity=25.8855585831063, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324225, Length=371, Percent_Identity=27.4932614555256, Blast_Score=106, Evalue=7e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42945; Mature: 42945

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMNREKVQIFDTTLRDGEQVPGCKLDTKQKLVIAERLDKMGVDIIEAGFPVSSPGDFLS
CCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
VSEICKIVENATVCGLTRAVKNDIDVAAAALKHAKKPRIHTGIGTSESHILHKLQTTPED
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCHHH
IIARAKFAVAHAKSYVEDVEFYAEDAGRTDNAFLAKVCEEVIKSGATVLNIPDTTGYCLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
EEYGAKIKYLKENVKGIENVILSCHCHNDLGMATANSIAGAINGARQIECTINGIGERAG
HHHCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCC
NTALEEVVMIFKQHPYLNLDTNINTRELNEMSRLVSESMGMIVQPNKAIVGANAFAHSSG
CHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCEEECCCCEEEECCHHHHCCC
IHQDGVIKNRATYEIMDPLDVGVNESSIILTARSGRAALAYRAKKVGYELTKTQLDTVYI
CCCCCCCCCCCCEEECCHHHCCCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHH
EFLKFADIKKEVVDADIHQIIEASKIEGELIRS
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MNMNREKVQIFDTTLRDGEQVPGCKLDTKQKLVIAERLDKMGVDIIEAGFPVSSPGDFLS
CCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
VSEICKIVENATVCGLTRAVKNDIDVAAAALKHAKKPRIHTGIGTSESHILHKLQTTPED
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCHHH
IIARAKFAVAHAKSYVEDVEFYAEDAGRTDNAFLAKVCEEVIKSGATVLNIPDTTGYCLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
EEYGAKIKYLKENVKGIENVILSCHCHNDLGMATANSIAGAINGARQIECTINGIGERAG
HHHCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCC
NTALEEVVMIFKQHPYLNLDTNINTRELNEMSRLVSESMGMIVQPNKAIVGANAFAHSSG
CHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCEEECCCCEEEECCHHHHCCC
IHQDGVIKNRATYEIMDPLDVGVNESSIILTARSGRAALAYRAKKVGYELTKTQLDTVYI
CCCCCCCCCCCCEEECCHHHCCCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHH
EFLKFADIKKEVVDADIHQIIEASKIEGELIRS
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]