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The map label for this gene is ilvD [H]

Identifier: 146300612

GI number: 146300612

Start: 3397030

End: 3398703

Strand: Reverse

Name: ilvD [H]

Synonym: Fjoh_2863

Alternate gene names: 146300612

Gene position: 3398703-3397030 (Counterclockwise)

Preceding gene: 146300613

Following gene: 146300611

Centisome position: 55.75

GC content: 39.55

Gene sequence:

>1674_bases
ATGGAATTAAATAAGTACAGCAAAACCATCACTCAAGATCAAACACAGCCTGCGGCGCAAGCAATGTTGTATGGAATTGG
TTTAACTGAAGAAGATTTAAAAAAAGCACAAGTTGGTATTGTCAGCATGGGTTATGATGGTAATACTTGCAATATGCACT
TAAATGATTTAGCAAAAGATGTTAAAAAAGGTGTTTGGGATGCTGGTCTGGTCGGACTTATTTTTAATACCATTGGTGTA
AGTGACGGAATTTCTAACGGTACAGAAGGAATGCGTTATTCATTAGTTTCTCGTGATGTAATCGCAGATTCTATTGAAAC
TGTAGCCGGTGCACAATGGTACGATAGTATTATTGCAATTCCTGGCTGCGATAAAAATATGCCTGGAGCATTAATCGCAA
TGGGAAGATTAAACCGTCCGTCAATGATGGTTTATGGAGGATCTATCCACTCAGGAAAATGGAAAGGTGAATCTCTAAAC
ATCGTTTCTGCTTTTGAAGCTTTAGGAAAAAAAGTAAAAGGCGAAATTACTCCGGAAGATTTTAAAGGTGTTATTCAAAA
TGCTTGTCCTGGAGCTGGTGCCTGCGGTGGTATGTATACTGCAAACACTATGTCTTCTGCAATTGAAGCATTAGGTATGA
GTATGCCTTACAGTTCTTCTAACCCTGCTTTGAGTCAGGAAAAAAGAGACGAATGTATTGCTGCCGGAGAAGCTATTAAA
GTTTTATTAGAAAAAGATATTAAGCCAAGAGATATTATGACTCGTAAAGCTTTCGAAAATGCTATTACAATTGTAGCAGT
TTTAGGAGGTTCTACAAACGCAGTTATGCACTTAATTGCAATGGCACACTCTGTTGGTATTACAATTACTCTTGATGATT
TTCAGGCAATTAACGACAGAACACCTGTTCTTGCCGATATGAAACCAAGTGGTAAATATATGATGGAAGACATTCACGAA
GTGGGAGGTATTCCGGGTGTAATGAAATATTTATTGAAAGTGGGACTTATCCACGGAGATTGTTTAACAGTAACTGGAAA
AACAGTTGCTGAAAACTTAGCTTCTACACCGGATTTACAAGACGGACAAGAAGTAATTCATGAAATTCAAAAAGCGTTAA
AACCAACAGGAAATATTCAGGTTTTATACGGAAACCTTGCTTCTGAAGGAGCTGTGGCAAAAATCAGCGGAAAAGAAGGG
GAATATTTCGAAGGACCAGCTGTTGTATTTGAAGGGGAATTTGAAGTAATTCCAGGCTTGCAGGCCGGAAAGATTAAACC
AGGTAATGTCGTCGTCATTAGAGGTTGTGGACCAAAAGGTGGTCCGGGAATGCCTGAAATGCTAAAACCGACATCTGCCA
TTATTGGTGCAGGATTAGGAAGCAGCTGTGCACTTATTACAGACGGCAGATTCTCCGGAGGTTCACACGGATTCGTGGTA
GGACACGTAACACCAGAGGCTTATGATGGAGGTGGTATTGCACTAGTAAAAGATGGAGATTTAATTGCTATCGATGCTGT
AAAAAATACAATCGACCTGAAAATATCTGACGAAGAATTTGCAGCTCGTAAAGCGGCTTGGGTTCAACCAGCGCTAAAAG
TTGACAGAGGGGTTTTACTTAAATACGCAAGATCGGTTTCTAGTGCTTCAACAGGCTGTGTTACCGATAATTAA

Upstream 100 bases:

>100_bases
ATCGGGAACGGTATAAATATAATTGAAAGACTATATTATAAACCTTCCAAATACTGGAAGGTTTTTTTTTGAATAAAATT
AAAATAAAAATAACGCAATA

Downstream 100 bases:

>100_bases
CCGACAAAAAATATGAGTTTGAAAACTGTAATTATTTCAAATTCTAAACAATAAAATTACCAAATGGGAACAATTAGAAT
ATCAGGCGCCGAAGCCGTTA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MELNKYSKTITQDQTQPAAQAMLYGIGLTEEDLKKAQVGIVSMGYDGNTCNMHLNDLAKDVKKGVWDAGLVGLIFNTIGV
SDGISNGTEGMRYSLVSRDVIADSIETVAGAQWYDSIIAIPGCDKNMPGALIAMGRLNRPSMMVYGGSIHSGKWKGESLN
IVSAFEALGKKVKGEITPEDFKGVIQNACPGAGACGGMYTANTMSSAIEALGMSMPYSSSNPALSQEKRDECIAAGEAIK
VLLEKDIKPRDIMTRKAFENAITIVAVLGGSTNAVMHLIAMAHSVGITITLDDFQAINDRTPVLADMKPSGKYMMEDIHE
VGGIPGVMKYLLKVGLIHGDCLTVTGKTVAENLASTPDLQDGQEVIHEIQKALKPTGNIQVLYGNLASEGAVAKISGKEG
EYFEGPAVVFEGEFEVIPGLQAGKIKPGNVVVIRGCGPKGGPGMPEMLKPTSAIIGAGLGSSCALITDGRFSGGSHGFVV
GHVTPEAYDGGGIALVKDGDLIAIDAVKNTIDLKISDEEFAARKAAWVQPALKVDRGVLLKYARSVSSASTGCVTDN

Sequences:

>Translated_557_residues
MELNKYSKTITQDQTQPAAQAMLYGIGLTEEDLKKAQVGIVSMGYDGNTCNMHLNDLAKDVKKGVWDAGLVGLIFNTIGV
SDGISNGTEGMRYSLVSRDVIADSIETVAGAQWYDSIIAIPGCDKNMPGALIAMGRLNRPSMMVYGGSIHSGKWKGESLN
IVSAFEALGKKVKGEITPEDFKGVIQNACPGAGACGGMYTANTMSSAIEALGMSMPYSSSNPALSQEKRDECIAAGEAIK
VLLEKDIKPRDIMTRKAFENAITIVAVLGGSTNAVMHLIAMAHSVGITITLDDFQAINDRTPVLADMKPSGKYMMEDIHE
VGGIPGVMKYLLKVGLIHGDCLTVTGKTVAENLASTPDLQDGQEVIHEIQKALKPTGNIQVLYGNLASEGAVAKISGKEG
EYFEGPAVVFEGEFEVIPGLQAGKIKPGNVVVIRGCGPKGGPGMPEMLKPTSAIIGAGLGSSCALITDGRFSGGSHGFVV
GHVTPEAYDGGGIALVKDGDLIAIDAVKNTIDLKISDEEFAARKAAWVQPALKVDRGVLLKYARSVSSASTGCVTDN
>Mature_557_residues
MELNKYSKTITQDQTQPAAQAMLYGIGLTEEDLKKAQVGIVSMGYDGNTCNMHLNDLAKDVKKGVWDAGLVGLIFNTIGV
SDGISNGTEGMRYSLVSRDVIADSIETVAGAQWYDSIIAIPGCDKNMPGALIAMGRLNRPSMMVYGGSIHSGKWKGESLN
IVSAFEALGKKVKGEITPEDFKGVIQNACPGAGACGGMYTANTMSSAIEALGMSMPYSSSNPALSQEKRDECIAAGEAIK
VLLEKDIKPRDIMTRKAFENAITIVAVLGGSTNAVMHLIAMAHSVGITITLDDFQAINDRTPVLADMKPSGKYMMEDIHE
VGGIPGVMKYLLKVGLIHGDCLTVTGKTVAENLASTPDLQDGQEVIHEIQKALKPTGNIQVLYGNLASEGAVAKISGKEG
EYFEGPAVVFEGEFEVIPGLQAGKIKPGNVVVIRGCGPKGGPGMPEMLKPTSAIIGAGLGSSCALITDGRFSGGSHGFVV
GHVTPEAYDGGGIALVKDGDLIAIDAVKNTIDLKISDEEFAARKAAWVQPALKVDRGVLLKYARSVSSASTGCVTDN

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=36.8421052631579, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI2367371, Length=457, Percent_Identity=35.4485776805252, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1788157, Length=554, Percent_Identity=30.6859205776173, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786464, Length=465, Percent_Identity=33.7634408602151, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322476, Length=563, Percent_Identity=56.1278863232682, Blast_Score=629, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 58476; Mature: 58476

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNKYSKTITQDQTQPAAQAMLYGIGLTEEDLKKAQVGIVSMGYDGNTCNMHLNDLAKD
CCCHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCEEEEEHHHHHHH
VKKGVWDAGLVGLIFNTIGVSDGISNGTEGMRYSLVSRDVIADSIETVAGAQWYDSIIAI
HHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHCEEEC
PGCDKNMPGALIAMGRLNRPSMMVYGGSIHSGKWKGESLNIVSAFEALGKKVKGEITPED
CCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
FKGVIQNACPGAGACGGMYTANTMSSAIEALGMSMPYSSSNPALSQEKRDECIAAGEAIK
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VLLEKDIKPRDIMTRKAFENAITIVAVLGGSTNAVMHLIAMAHSVGITITLDDFQAINDR
HHHHCCCCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCHHCCCC
TPVLADMKPSGKYMMEDIHEVGGIPGVMKYLLKVGLIHGDCLTVTGKTVAENLASTPDLQ
CCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCC
DGQEVIHEIQKALKPTGNIQVLYGNLASEGAVAKISGKEGEYFEGPAVVFEGEFEVIPGL
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCEEEEECCEEECCCC
QAGKIKPGNVVVIRGCGPKGGPGMPEMLKPTSAIIGAGLGSSCALITDGRFSGGSHGFVV
CCCCCCCCCEEEEECCCCCCCCCCHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCEEE
GHVTPEAYDGGGIALVKDGDLIAIDAVKNTIDLKISDEEFAARKAAWVQPALKVDRGVLL
EECCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHH
KYARSVSSASTGCVTDN
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MELNKYSKTITQDQTQPAAQAMLYGIGLTEEDLKKAQVGIVSMGYDGNTCNMHLNDLAKD
CCCHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCCEEEEEHHHHHHH
VKKGVWDAGLVGLIFNTIGVSDGISNGTEGMRYSLVSRDVIADSIETVAGAQWYDSIIAI
HHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHCEEEC
PGCDKNMPGALIAMGRLNRPSMMVYGGSIHSGKWKGESLNIVSAFEALGKKVKGEITPED
CCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
FKGVIQNACPGAGACGGMYTANTMSSAIEALGMSMPYSSSNPALSQEKRDECIAAGEAIK
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VLLEKDIKPRDIMTRKAFENAITIVAVLGGSTNAVMHLIAMAHSVGITITLDDFQAINDR
HHHHCCCCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCHHCCCC
TPVLADMKPSGKYMMEDIHEVGGIPGVMKYLLKVGLIHGDCLTVTGKTVAENLASTPDLQ
CCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCC
DGQEVIHEIQKALKPTGNIQVLYGNLASEGAVAKISGKEGEYFEGPAVVFEGEFEVIPGL
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCEEEEECCEEECCCC
QAGKIKPGNVVVIRGCGPKGGPGMPEMLKPTSAIIGAGLGSSCALITDGRFSGGSHGFVV
CCCCCCCCCEEEEECCCCCCCCCCHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCEEE
GHVTPEAYDGGGIALVKDGDLIAIDAVKNTIDLKISDEEFAARKAAWVQPALKVDRGVLL
EECCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHH
KYARSVSSASTGCVTDN
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA