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The map label for this gene is ilvB [H]

Identifier: 146300611

GI number: 146300611

Start: 3395272

End: 3396966

Strand: Reverse

Name: ilvB [H]

Synonym: Fjoh_2862

Alternate gene names: 146300611

Gene position: 3396966-3395272 (Counterclockwise)

Preceding gene: 146300612

Following gene: 146300610

Centisome position: 55.72

GC content: 37.29

Gene sequence:

>1695_bases
ATGGGAACAATTAGAATATCAGGCGCCGAAGCCGTTATTAGATGCTTATTAGAAGAAGGAGTAGACTTAATTTATGGGTA
TCCGGGAGGAGCTATAATGCCGGTTTACGACGAATTATATAAATTTCAGGATCAGCTGCACCACGTTTTAGTACGTCACG
AACAAGGTGCAACGCATGCTGCTCAGGGATATGCGAGAGCAACAGGAAAAGTGGGTGTTGCAATTGCAACTTCTGGACCG
GGAGCTACAAATCTTGTAACTGGAATTGCAGATGCTCAGATCGACTCAACTCCAATGGTTTGTATTACAGGACAAGTTGG
AAGACATTTATTAGGATCTGATGCTTTTCAGGAAACGGATATCATCGGAATTTCAACTCCGGTTACAAAATGGAACTTTC
AGGTAACCGAAGCTTCTCAAATTCCTGAAATTATTGCAAAAGCATTTTATATCGCTCGTTCTGGACGTCCAGGGCCTGTT
TTAATCGACATTACTAAAAATGCTCAGTTTGATGAGTTTGATTTTAGTTATGAAAAATGTACTGGAATTAGAAGTTATAA
TCCGGTTCCGAAATTAAATTTAGATAAAGTTGCCGAAGCTGCAGCCTTAATTAATAGTGCTAAAAAACCTTTTATCGTTT
TCGGTCAGGGAATTATATTAGGTCAGGCTGAAGCTGAATTTAAGGCAGTTCTTGAAAAATCAGGAATTCCGGCTGCATGG
ACTATTTTAGGACTTTCGGCTCTGCCAACAGATCATCCTTTAAATGTTGGTATGTTAGGAATGCACGGTAATTATGCACC
AAACTTATTAACTAACGAATGTGATGTATTAATTGCTTTCGGTATGCGTTTTGACGATCGTGTAACGGGTAATTTAAAAA
CTTATGCAAAACAGGCAAAAGTAATTCACTTCGAAATTGATCCTGCAGAGGTTGATAAAAACGTTAAAACAGAAATTGCT
GTTTTAGGTGATGTAAAAGAATCTTTAGCTGCTTTATTGCCGCTGCTTGATGCAAAATCACACGATTTATGGCACAATGA
ATTTAAAGAATTGGCGAAAATTGAATTTGATTCGGTAATTAAAGACGAATTAAATCCATCATCAGCTGGTCTTTCGATGG
GAGAAACTATTGAAATGATTAACAAACACTCAAAAGGTGATGCAATTATAGTTTCAGACGTAGGACAGCATCAAATGTTT
GCCTGCCGTTATGCAAAATTCAATTCAACAAAAAGTAATATTACTTCTGGCGGATTAGGAACAATGGGATTTGCGCTTCC
GGCTGCAATTGGAGCTAAAATGGGAAGACCAGACCGTGAAGTTGTTGCTATTATTGGCGATGGAGGTTTTCAGATGACAA
TTCAGGAATTAGGAACGATTTTTCAGACTCAGGTTCCTGTAAAAATTGTAATTCTAAATAATGAGTTTTTAGGAATGGTA
CGTCAATGGCAGGAATTGTTCTTTGATAACAGATACGCTTCAACAAAAATGATTAATCCAAATTTTGTTGCCATTGCCGA
AGGATATCATATTAAATCTAAAAAAGTAACACAGCGAGAAGATTTAGATGCTGCTGTTGCAGAAATGCTGGCTTCAAAAG
ATTCTTATTTCTTAGAAGTTATGGTGGAAAAAGAAAACAACGTTTTCCCAATGATTCCAACCGGAGCGTGTGTTTCAGAA
ATTAGATTAAGTTAA

Upstream 100 bases:

>100_bases
TTCTAGTGCTTCAACAGGCTGTGTTACCGATAATTAACCGACAAAAAATATGAGTTTGAAAACTGTAATTATTTCAAATT
CTAAACAATAAAATTACCAA

Downstream 100 bases:

>100_bases
GAAAAAGTTTCAGGTTTCAAGTTTCAGGTTTCACGTTCTCAACAGAACTTGAAACCTGAAACCTGAAACAAATAAAACTA
AAAAAACAAAATGGAAGAGA

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MGTIRISGAEAVIRCLLEEGVDLIYGYPGGAIMPVYDELYKFQDQLHHVLVRHEQGATHAAQGYARATGKVGVAIATSGP
GATNLVTGIADAQIDSTPMVCITGQVGRHLLGSDAFQETDIIGISTPVTKWNFQVTEASQIPEIIAKAFYIARSGRPGPV
LIDITKNAQFDEFDFSYEKCTGIRSYNPVPKLNLDKVAEAAALINSAKKPFIVFGQGIILGQAEAEFKAVLEKSGIPAAW
TILGLSALPTDHPLNVGMLGMHGNYAPNLLTNECDVLIAFGMRFDDRVTGNLKTYAKQAKVIHFEIDPAEVDKNVKTEIA
VLGDVKESLAALLPLLDAKSHDLWHNEFKELAKIEFDSVIKDELNPSSAGLSMGETIEMINKHSKGDAIIVSDVGQHQMF
ACRYAKFNSTKSNITSGGLGTMGFALPAAIGAKMGRPDREVVAIIGDGGFQMTIQELGTIFQTQVPVKIVILNNEFLGMV
RQWQELFFDNRYASTKMINPNFVAIAEGYHIKSKKVTQREDLDAAVAEMLASKDSYFLEVMVEKENNVFPMIPTGACVSE
IRLS

Sequences:

>Translated_564_residues
MGTIRISGAEAVIRCLLEEGVDLIYGYPGGAIMPVYDELYKFQDQLHHVLVRHEQGATHAAQGYARATGKVGVAIATSGP
GATNLVTGIADAQIDSTPMVCITGQVGRHLLGSDAFQETDIIGISTPVTKWNFQVTEASQIPEIIAKAFYIARSGRPGPV
LIDITKNAQFDEFDFSYEKCTGIRSYNPVPKLNLDKVAEAAALINSAKKPFIVFGQGIILGQAEAEFKAVLEKSGIPAAW
TILGLSALPTDHPLNVGMLGMHGNYAPNLLTNECDVLIAFGMRFDDRVTGNLKTYAKQAKVIHFEIDPAEVDKNVKTEIA
VLGDVKESLAALLPLLDAKSHDLWHNEFKELAKIEFDSVIKDELNPSSAGLSMGETIEMINKHSKGDAIIVSDVGQHQMF
ACRYAKFNSTKSNITSGGLGTMGFALPAAIGAKMGRPDREVVAIIGDGGFQMTIQELGTIFQTQVPVKIVILNNEFLGMV
RQWQELFFDNRYASTKMINPNFVAIAEGYHIKSKKVTQREDLDAAVAEMLASKDSYFLEVMVEKENNVFPMIPTGACVSE
IRLS
>Mature_563_residues
GTIRISGAEAVIRCLLEEGVDLIYGYPGGAIMPVYDELYKFQDQLHHVLVRHEQGATHAAQGYARATGKVGVAIATSGPG
ATNLVTGIADAQIDSTPMVCITGQVGRHLLGSDAFQETDIIGISTPVTKWNFQVTEASQIPEIIAKAFYIARSGRPGPVL
IDITKNAQFDEFDFSYEKCTGIRSYNPVPKLNLDKVAEAAALINSAKKPFIVFGQGIILGQAEAEFKAVLEKSGIPAAWT
ILGLSALPTDHPLNVGMLGMHGNYAPNLLTNECDVLIAFGMRFDDRVTGNLKTYAKQAKVIHFEIDPAEVDKNVKTEIAV
LGDVKESLAALLPLLDAKSHDLWHNEFKELAKIEFDSVIKDELNPSSAGLSMGETIEMINKHSKGDAIIVSDVGQHQMFA
CRYAKFNSTKSNITSGGLGTMGFALPAAIGAKMGRPDREVVAIIGDGGFQMTIQELGTIFQTQVPVKIVILNNEFLGMVR
QWQELFFDNRYASTKMINPNFVAIAEGYHIKSKKVTQREDLDAAVAEMLASKDSYFLEVMVEKENNVFPMIPTGACVSEI
RLS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=25.4416961130742, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI21361361, Length=504, Percent_Identity=24.8015873015873, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=40.8695652173913, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=41.9354838709677, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=568, Percent_Identity=32.0422535211268, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=26.7272727272727, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1788716, Length=479, Percent_Identity=25.8872651356994, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=480, Percent_Identity=28.75, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=480, Percent_Identity=28.75, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=587, Percent_Identity=24.7018739352641, Blast_Score=131, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=592, Percent_Identity=39.3581081081081, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6320816, Length=481, Percent_Identity=23.0769230769231, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320588, Length=605, Percent_Identity=22.3140495867769, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=503, Percent_Identity=24.2544731610338, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=27.8350515463918, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61332; Mature: 61201

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTIRISGAEAVIRCLLEEGVDLIYGYPGGAIMPVYDELYKFQDQLHHVLVRHEQGATHA
CCEEEECCHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHH
AQGYARATGKVGVAIATSGPGATNLVTGIADAQIDSTPMVCITGQVGRHLLGSDAFQETD
HCCHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEECHHHHHHHCCCCCCCCC
IIGISTPVTKWNFQVTEASQIPEIIAKAFYIARSGRPGPVLIDITKNAQFDEFDFSYEKC
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHH
TGIRSYNPVPKLNLDKVAEAAALINSAKKPFIVFGQGIILGQAEAEFKAVLEKSGIPAAW
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECEEEEECCHHHHHHHHHHCCCCEEE
TILGLSALPTDHPLNVGMLGMHGNYAPNLLTNECDVLIAFGMRFDDRVTGNLKTYAKQAK
HEEEHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCE
VIHFEIDPAEVDKNVKTEIAVLGDVKESLAALLPLLDAKSHDLWHNEFKELAKIEFDSVI
EEEEECCHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KDELNPSSAGLSMGETIEMINKHSKGDAIIVSDVGQHQMFACRYAKFNSTKSNITSGGLG
HHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEEEHHHCCCCCCCCCCCCCC
TMGFALPAAIGAKMGRPDREVVAIIGDGGFQMTIQELGTIFQTQVPVKIVILNNEFLGMV
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCEEEEEECCHHHHHH
RQWQELFFDNRYASTKMINPNFVAIAEGYHIKSKKVTQREDLDAAVAEMLASKDSYFLEV
HHHHHHHHCCCCCCCEECCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEE
MVEKENNVFPMIPTGACVSEIRLS
EEECCCCEEEECCCCHHHHHHCCC
>Mature Secondary Structure 
GTIRISGAEAVIRCLLEEGVDLIYGYPGGAIMPVYDELYKFQDQLHHVLVRHEQGATHA
CEEEECCHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHH
AQGYARATGKVGVAIATSGPGATNLVTGIADAQIDSTPMVCITGQVGRHLLGSDAFQETD
HCCHHHCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEECHHHHHHHCCCCCCCCC
IIGISTPVTKWNFQVTEASQIPEIIAKAFYIARSGRPGPVLIDITKNAQFDEFDFSYEKC
EEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHH
TGIRSYNPVPKLNLDKVAEAAALINSAKKPFIVFGQGIILGQAEAEFKAVLEKSGIPAAW
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECEEEEECCHHHHHHHHHHCCCCEEE
TILGLSALPTDHPLNVGMLGMHGNYAPNLLTNECDVLIAFGMRFDDRVTGNLKTYAKQAK
HEEEHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCE
VIHFEIDPAEVDKNVKTEIAVLGDVKESLAALLPLLDAKSHDLWHNEFKELAKIEFDSVI
EEEEECCHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KDELNPSSAGLSMGETIEMINKHSKGDAIIVSDVGQHQMFACRYAKFNSTKSNITSGGLG
HHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEEEHHHCCCCCCCCCCCCCC
TMGFALPAAIGAKMGRPDREVVAIIGDGGFQMTIQELGTIFQTQVPVKIVILNNEFLGMV
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCEEEEEECCHHHHHH
RQWQELFFDNRYASTKMINPNFVAIAEGYHIKSKKVTQREDLDAAVAEMLASKDSYFLEV
HHHHHHHHCCCCCCCEECCCCEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEE
MVEKENNVFPMIPTGACVSEIRLS
EEECCCCEEEECCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]