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The map label for this gene is nagB [C]

Identifier: 146300136

GI number: 146300136

Start: 2852757

End: 2854685

Strand: Direct

Name: nagB [C]

Synonym: Fjoh_2381

Alternate gene names: 146300136

Gene position: 2852757-2854685 (Clockwise)

Preceding gene: 146300135

Following gene: 146300137

Centisome position: 46.79

GC content: 37.22

Gene sequence:

>1929_bases
ATGTTAAAAAGTAAAATAGACAAAGCAACCGGTTTTGAAAAACGCTTCGAAAACATCAATACCGTTGTTTTTGAAAATTC
AAACGAAGCTTCAAAAGCAGTTGCACAGGAAATCGCAGCTTTAATTCAATCAAAACAAAAAGAAAACAAGCCTTGTATTT
TAGGTTTGGCTACAGGTTCTTCTCCAAAAGGTTTGTATGCTGAATTAGTGCGTCTGCATAAAGAAGAAGGTTTGAGTTTT
AAAAACGTAATCAGTTTTAATCTTGACGAATATTACCCGATGGAGCCAAACTCTATCAATAGTTATGTTCGTTTTATGAA
AGAATTATTGTTTGATCATGTGGATATTTTACCTGAAAATGCTCATGTTCCAGACGGACTTTTAACTAAAGAACAAATTG
CAGACTACTGCCACGAATATGAGGCTAAAATTGAAGCCCTGGGCGGCATTGATTTGCAGATTCTTGGAATTGGAGGAAAC
GGACATATTGGTTTTAATGAATCAGGATCGCTTCAAAACTCAAAAACACGTTTAGTAGCTTTGGATCATATTACAAGAGT
TGCTGCAAGTAAAGATTTCTTCGGATTAAATAATACGCCAAGAACTGCAATTACACTTGGGGTTAAAAAAATCATGGAGG
CTAAAAGAGTTATTTTATTAGCTTGGGGTGAAGGAAAAGCCAATATTGTAAAAAAATCGGTTGAAGATGAAGTTACAAAC
AGAGTTCCGGCTTCATTCCTGCAAGAACACGATAATGCAGTTTTTATTTTAGATAAAGAAGCTTCTTCAAAACTTACAAG
TATCAACAAACCATGGCTTGTAGAGAAAATTGTCTGGACAGATAAATTAACTCGTAAAGCAGTTTTAGGATTGGCTCTGG
ATCTTAAAAAACCAATTTTAATGCTTACTGATGCTGATTATATCGAAAACGGAATGAGCGATTTATTAGCAGATTCTGGT
CCGGCATACGATATCAATATCAAGATATTCAATAAATTACAAAATACAATTACAGGCTGGCCGGGCGGAAAACCAAATGC
AGAAGATGCAAATCGTCCTGAAAGAGCTGAGCCTGCTAAAAAGCGCGTATTGATTTTTAGTCCGCATCCAGATGATGATA
TTATTAGTATGGGAGGAACTTTTATGCGTTTGCAGGAGCAGGGGCATGAAGTGCACGTAGCTTATCAGACATCAGGGAAT
ATTGCTGTTGCCGATGATGAAGCATTGCGTTTTGCCAGATTCGTAATTGATTACAATGAAAAATTCGGAATCAAGAGCGA
AGAAGCTGATAACATTTATCAAAAAGCACAAACCTTTTTAGAAAATAAAAAGAACAGTGAAATTGATATTCCTGAAGTAC
GATATATAAAAGGATTAATTAGAAAAGGAGAAGCAAGAGCAACAAGTCATTTTGTTGGTCTGCCAGACAGTCAGATTCAT
TTTATGGAACTACCTTTCTATGAAACGGGAACAATTGAGAAAAAACCAATTGGACCAGAAGATATTCAGTTAACAGTAGA
TTTAATTGAAAAAATTAAACCGCACCAAATCTACGCAGCAGGAGATTTAGCAGATCCGCACGGAACACACAAAGTTTGTC
TGGATGCTATTTTTGAAGCTGTAAAAGTTTTAAAACCAAAAGAGTTTATGAACGACTGCTGGTTATGGTTATACCGCGGT
GCTTGGCAGGAATGGGGAATTGATGAAGTAGAAATGGCGGTACCAATGAGCCCGGATCAGGTTTTGGCAAAACGTCACGG
AATCTTCAAGCACCAGTCGCAAAAAGACGGTGTTGTTTTTCAGGGAACTGATGCAAGAGAGTTCTGGCAGAGAGCCGAAG
ACCGAAACAGCGAAACAGCAGCTTTGTATCAGCAATTAGGTTTGGCAAGTTATGCTGCAATGGAAGCTTTTGTGAGATGG
CATTATTAA

Upstream 100 bases:

>100_bases
TATTTTAGCAATGATTTGACTTGCGTTTTTTCAATTGAAAAATGAAATTTTAGGGTTTTAACCCAGCACACATAATCTGA
AGAATAAAAAAAACTATATC

Downstream 100 bases:

>100_bases
GATGCTAAGCTTTTTAGTTGTCAGTCGGAGTCACAGTTTGCAGTGTCAATGAGCAGTCAATTTGATACTGAGACTGAAAA
ACAAGACTGAAAACTATTTT

Product: glucosamine-6-phosphate deaminase-like protein

Products: D-fructose 6-phosphate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MLKSKIDKATGFEKRFENINTVVFENSNEASKAVAQEIAALIQSKQKENKPCILGLATGSSPKGLYAELVRLHKEEGLSF
KNVISFNLDEYYPMEPNSINSYVRFMKELLFDHVDILPENAHVPDGLLTKEQIADYCHEYEAKIEALGGIDLQILGIGGN
GHIGFNESGSLQNSKTRLVALDHITRVAASKDFFGLNNTPRTAITLGVKKIMEAKRVILLAWGEGKANIVKKSVEDEVTN
RVPASFLQEHDNAVFILDKEASSKLTSINKPWLVEKIVWTDKLTRKAVLGLALDLKKPILMLTDADYIENGMSDLLADSG
PAYDINIKIFNKLQNTITGWPGGKPNAEDANRPERAEPAKKRVLIFSPHPDDDIISMGGTFMRLQEQGHEVHVAYQTSGN
IAVADDEALRFARFVIDYNEKFGIKSEEADNIYQKAQTFLENKKNSEIDIPEVRYIKGLIRKGEARATSHFVGLPDSQIH
FMELPFYETGTIEKKPIGPEDIQLTVDLIEKIKPHQIYAAGDLADPHGTHKVCLDAIFEAVKVLKPKEFMNDCWLWLYRG
AWQEWGIDEVEMAVPMSPDQVLAKRHGIFKHQSQKDGVVFQGTDAREFWQRAEDRNSETAALYQQLGLASYAAMEAFVRW
HY

Sequences:

>Translated_642_residues
MLKSKIDKATGFEKRFENINTVVFENSNEASKAVAQEIAALIQSKQKENKPCILGLATGSSPKGLYAELVRLHKEEGLSF
KNVISFNLDEYYPMEPNSINSYVRFMKELLFDHVDILPENAHVPDGLLTKEQIADYCHEYEAKIEALGGIDLQILGIGGN
GHIGFNESGSLQNSKTRLVALDHITRVAASKDFFGLNNTPRTAITLGVKKIMEAKRVILLAWGEGKANIVKKSVEDEVTN
RVPASFLQEHDNAVFILDKEASSKLTSINKPWLVEKIVWTDKLTRKAVLGLALDLKKPILMLTDADYIENGMSDLLADSG
PAYDINIKIFNKLQNTITGWPGGKPNAEDANRPERAEPAKKRVLIFSPHPDDDIISMGGTFMRLQEQGHEVHVAYQTSGN
IAVADDEALRFARFVIDYNEKFGIKSEEADNIYQKAQTFLENKKNSEIDIPEVRYIKGLIRKGEARATSHFVGLPDSQIH
FMELPFYETGTIEKKPIGPEDIQLTVDLIEKIKPHQIYAAGDLADPHGTHKVCLDAIFEAVKVLKPKEFMNDCWLWLYRG
AWQEWGIDEVEMAVPMSPDQVLAKRHGIFKHQSQKDGVVFQGTDAREFWQRAEDRNSETAALYQQLGLASYAAMEAFVRW
HY
>Mature_642_residues
MLKSKIDKATGFEKRFENINTVVFENSNEASKAVAQEIAALIQSKQKENKPCILGLATGSSPKGLYAELVRLHKEEGLSF
KNVISFNLDEYYPMEPNSINSYVRFMKELLFDHVDILPENAHVPDGLLTKEQIADYCHEYEAKIEALGGIDLQILGIGGN
GHIGFNESGSLQNSKTRLVALDHITRVAASKDFFGLNNTPRTAITLGVKKIMEAKRVILLAWGEGKANIVKKSVEDEVTN
RVPASFLQEHDNAVFILDKEASSKLTSINKPWLVEKIVWTDKLTRKAVLGLALDLKKPILMLTDADYIENGMSDLLADSG
PAYDINIKIFNKLQNTITGWPGGKPNAEDANRPERAEPAKKRVLIFSPHPDDDIISMGGTFMRLQEQGHEVHVAYQTSGN
IAVADDEALRFARFVIDYNEKFGIKSEEADNIYQKAQTFLENKKNSEIDIPEVRYIKGLIRKGEARATSHFVGLPDSQIH
FMELPFYETGTIEKKPIGPEDIQLTVDLIEKIKPHQIYAAGDLADPHGTHKVCLDAIFEAVKVLKPKEFMNDCWLWLYRG
AWQEWGIDEVEMAVPMSPDQVLAKRHGIFKHQSQKDGVVFQGTDAREFWQRAEDRNSETAALYQQLGLASYAAMEAFVRW
HY

Specific function: Catalyzes The Reversible Isomerization-Deamination Of Glucosamine 6-Phosphate (Glcn6p) To Form Fructose 6-Phosphate (Fru6p) And Ammonium Ion. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=246, Percent_Identity=40.2439024390244, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI13027378, Length=246, Percent_Identity=39.0243902439024, Blast_Score=159, Evalue=6e-39,
Organism=Escherichia coli, GI1786893, Length=237, Percent_Identity=37.5527426160338, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789530, Length=248, Percent_Identity=27.8225806451613, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI48994958, Length=238, Percent_Identity=26.890756302521, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554876, Length=246, Percent_Identity=36.1788617886179, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24581960, Length=247, Percent_Identity=36.4372469635627, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI19920764, Length=247, Percent_Identity=36.4372469635627, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR003737
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso; PF02585 PIG-L [H]

EC number: 3.5.99.6

Molecular weight: Translated: 72204; Mature: 72204

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSKIDKATGFEKRFENINTVVFENSNEASKAVAQEIAALIQSKQKENKPCILGLATGS
CCCCHHHHHCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
SPKGLYAELVRLHKEEGLSFKNVISFNLDEYYPMEPNSINSYVRFMKELLFDHVDILPEN
CCCHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AHVPDGLLTKEQIADYCHEYEAKIEALGGIDLQILGIGGNGHIGFNESGSLQNSKTRLVA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEE
LDHITRVAASKDFFGLNNTPRTAITLGVKKIMEAKRVILLAWGEGKANIVKKSVEDEVTN
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
RVPASFLQEHDNAVFILDKEASSKLTSINKPWLVEKIVWTDKLTRKAVLGLALDLKKPIL
CCCHHHHHHCCCEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
MLTDADYIENGMSDLLADSGPAYDINIKIFNKLQNTITGWPGGKPNAEDANRPERAEPAK
EEECCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
KRVLIFSPHPDDDIISMGGTFMRLQEQGHEVHVAYQTSGNIAVADDEALRFARFVIDYNE
CEEEEECCCCCCHHHHCCCHHHHHHHCCCEEEEEEECCCCEEEECHHHHHHHHHHEECCC
KFGIKSEEADNIYQKAQTFLENKKNSEIDIPEVRYIKGLIRKGEARATSHFVGLPDSQIH
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHEEECCCCCCEE
FMELPFYETGTIEKKPIGPEDIQLTVDLIEKIKPHQIYAAGDLADPHGTHKVCLDAIFEA
EEECCCCCCCCCCCCCCCCCHHEEHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
VKVLKPKEFMNDCWLWLYRGAWQEWGIDEVEMAVPMSPDQVLAKRHGIFKHQSQKDGVVF
HHHCCCHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEE
QGTDAREFWQRAEDRNSETAALYQQLGLASYAAMEAFVRWHY
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKSKIDKATGFEKRFENINTVVFENSNEASKAVAQEIAALIQSKQKENKPCILGLATGS
CCCCHHHHHCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
SPKGLYAELVRLHKEEGLSFKNVISFNLDEYYPMEPNSINSYVRFMKELLFDHVDILPEN
CCCHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
AHVPDGLLTKEQIADYCHEYEAKIEALGGIDLQILGIGGNGHIGFNESGSLQNSKTRLVA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEE
LDHITRVAASKDFFGLNNTPRTAITLGVKKIMEAKRVILLAWGEGKANIVKKSVEDEVTN
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHH
RVPASFLQEHDNAVFILDKEASSKLTSINKPWLVEKIVWTDKLTRKAVLGLALDLKKPIL
CCCHHHHHHCCCEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
MLTDADYIENGMSDLLADSGPAYDINIKIFNKLQNTITGWPGGKPNAEDANRPERAEPAK
EEECCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
KRVLIFSPHPDDDIISMGGTFMRLQEQGHEVHVAYQTSGNIAVADDEALRFARFVIDYNE
CEEEEECCCCCCHHHHCCCHHHHHHHCCCEEEEEEECCCCEEEECHHHHHHHHHHEECCC
KFGIKSEEADNIYQKAQTFLENKKNSEIDIPEVRYIKGLIRKGEARATSHFVGLPDSQIH
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHEEECCCCCCEE
FMELPFYETGTIEKKPIGPEDIQLTVDLIEKIKPHQIYAAGDLADPHGTHKVCLDAIFEA
EEECCCCCCCCCCCCCCCCCHHEEHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
VKVLKPKEFMNDCWLWLYRGAWQEWGIDEVEMAVPMSPDQVLAKRHGIFKHQSQKDGVVF
HHHCCCHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEE
QGTDAREFWQRAEDRNSETAALYQQLGLASYAAMEAFVRWHY
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucosamine 6-phosphate; H2O

Specific reaction: D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3

General reaction: Group transfer (transmolecular Intramolecular oxidoreduction) [C]

Inhibitor: N3-(4-Methoxyfumaroyl)-L-2, 3-diaminopropanoic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]