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The map label for this gene is prfA

Identifier: 146300064

GI number: 146300064

Start: 2778076

End: 2779152

Strand: Direct

Name: prfA

Synonym: Fjoh_2309

Alternate gene names: 146300064

Gene position: 2778076-2779152 (Clockwise)

Preceding gene: 146300057

Following gene: 146300065

Centisome position: 45.57

GC content: 40.48

Gene sequence:

>1077_bases
ATGTTAGATAGACTTCAATATGTAAAGCAGCGTTTCGATGAGATTTCGGATTTGATTATTCAGCCGGATGTTATTTCTGA
TCAAAAACGTTACAAGTCGCTTAACCAGGAATATAAAGGTATAAAAGCGCTTGTTGAAAAGAGAGAAGAATACATTATAG
TTTTAGCAAACATCGACGAAGCAAACGAAATTATTGCTGACGGAAGCGATGCTGAAATGGTAGAAATGGCCAAAATGCAG
TTGGATGAAGCAAAAGACCGTTTACCGGAACTGGAGGAGGAAATCAAATTTATGCTGATTCCTAAAGATCCAGAAGATGC
GAAAAACGTTATGGTGGAGATCCGCGCCGGAACGGGTGGGGACGAAGCGAGTATTTTTGCTGGTGACTTGTTTAGAATGT
ACACAAAATACTGTGAAACAATGGGATGGAGAACATCTGTTGTTGATATGAACGAAGGAACTTCTGGAGGTTTCAAAGAG
GTTATTTTTGAAGTTACAGGAGAAGATGTTTACGGAACTTTGAAATTTGAAGCAGGTGTTCACCGTGTACAGCGTGTTCC
GCAGACAGAAACTCAGGGACGTGTACATACATCGGCTGCAACGGTTATGGTTTTGCCAGAAGCTGAGGAGTTTGATGTTC
AGGTAGATATGAACGATGTTCGTGTAGATTTCTTCTGTTCATCAGGACCTGGAGGACAGTCGGTAAATACAACGAAATCG
GCTGTACGTTTAACGCACATTCCAACTGGATTAGTTGCGCAGTGTCAGGATGAGAAATCACAGCACAAGAATAAAGATAA
AGCTTTAATGGTATTGCGTTCTCGTTTATACGAAATGGAATTGGCTAAAAAGCAGGAAGAAGATGCTAAAAAACGTAGTT
CTCAGGTAAGTTCTGGAGACCGTTCGGCTAAAATTCGTACGTATAACTATGCACAAGGCCGTGTAACCGATCACCGTGTT
GGTTTAACGCTTTACGATTTAGGAAACATCATGAATGGTGATATTCAGAAAATCGTTTCTGAACTTCAGCTGGTTAATAA
CATGGAGAAATTGAAGGAAGCTTCGGAAGTGTATTAA

Upstream 100 bases:

>100_bases
AATAATCGATCTAAATAAATCCCAAAACACACAAAAAATCAACACTAGAATTTTCCACAAAAATTTTGTAATTTTGCCGT
CCAATAAAAGGAATTAGAAA

Downstream 100 bases:

>100_bases
TCGTAGAGGTTCAAAGTGACAGAGTTACAAAGGTTCAAAGGTTTTCTTTGAACATAAATTATATTTAAAGCCGGACATTT
GTTCGGCTTTTTTTTAATGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLDRLQYVKQRFDEISDLIIQPDVISDQKRYKSLNQEYKGIKALVEKREEYIIVLANIDEANEIIADGSDAEMVEMAKMQ
LDEAKDRLPELEEEIKFMLIPKDPEDAKNVMVEIRAGTGGDEASIFAGDLFRMYTKYCETMGWRTSVVDMNEGTSGGFKE
VIFEVTGEDVYGTLKFEAGVHRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQVDMNDVRVDFFCSSGPGGQSVNTTKS
AVRLTHIPTGLVAQCQDEKSQHKNKDKALMVLRSRLYEMELAKKQEEDAKKRSSQVSSGDRSAKIRTYNYAQGRVTDHRV
GLTLYDLGNIMNGDIQKIVSELQLVNNMEKLKEASEVY

Sequences:

>Translated_358_residues
MLDRLQYVKQRFDEISDLIIQPDVISDQKRYKSLNQEYKGIKALVEKREEYIIVLANIDEANEIIADGSDAEMVEMAKMQ
LDEAKDRLPELEEEIKFMLIPKDPEDAKNVMVEIRAGTGGDEASIFAGDLFRMYTKYCETMGWRTSVVDMNEGTSGGFKE
VIFEVTGEDVYGTLKFEAGVHRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQVDMNDVRVDFFCSSGPGGQSVNTTKS
AVRLTHIPTGLVAQCQDEKSQHKNKDKALMVLRSRLYEMELAKKQEEDAKKRSSQVSSGDRSAKIRTYNYAQGRVTDHRV
GLTLYDLGNIMNGDIQKIVSELQLVNNMEKLKEASEVY
>Mature_358_residues
MLDRLQYVKQRFDEISDLIIQPDVISDQKRYKSLNQEYKGIKALVEKREEYIIVLANIDEANEIIADGSDAEMVEMAKMQ
LDEAKDRLPELEEEIKFMLIPKDPEDAKNVMVEIRAGTGGDEASIFAGDLFRMYTKYCETMGWRTSVVDMNEGTSGGFKE
VIFEVTGEDVYGTLKFEAGVHRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQVDMNDVRVDFFCSSGPGGQSVNTTKS
AVRLTHIPTGLVAQCQDEKSQHKNKDKALMVLRSRLYEMELAKKQEEDAKKRSSQVSSGDRSAKIRTYNYAQGRVTDHRV
GLTLYDLGNIMNGDIQKIVSELQLVNNMEKLKEASEVY

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=315, Percent_Identity=39.0476190476191, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI34577120, Length=310, Percent_Identity=35.8064516129032, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=36.3636363636364, Blast_Score=151, Evalue=8e-37,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=46.218487394958, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=33.7349397590361, Blast_Score=215, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=292, Percent_Identity=34.9315068493151, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=331, Percent_Identity=40.785498489426, Blast_Score=229, Evalue=5e-61,
Organism=Drosophila melanogaster, GI19921226, Length=372, Percent_Identity=36.0215053763441, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_FLAJ1 (A5FHI8)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194655.1
- STRING:   A5FHI8
- GeneID:   5094444
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2309
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   FJOH376686:FJOH_2309-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40492; Mature: 40492

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLQYVKQRFDEISDLIIQPDVISDQKRYKSLNQEYKGIKALVEKREEYIIVLANIDE
CCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
ANEIIADGSDAEMVEMAKMQLDEAKDRLPELEEEIKFMLIPKDPEDAKNVMVEIRAGTGG
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCEEEEEECCCCC
DEASIFAGDLFRMYTKYCETMGWRTSVVDMNEGTSGGFKEVIFEVTGEDVYGTLKFEAGV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEHHHH
HRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQVDMNDVRVDFFCSSGPGGQSVNTTKS
HHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCEEEEEEEECCCCCCCCCCCHHH
AVRLTHIPTGLVAQCQDEKSQHKNKDKALMVLRSRLYEMELAKKQEEDAKKRSSQVSSGD
HEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSAKIRTYNYAQGRVTDHRVGLTLYDLGNIMNGDIQKIVSELQLVNNMEKLKEASEVY
CCCEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDRLQYVKQRFDEISDLIIQPDVISDQKRYKSLNQEYKGIKALVEKREEYIIVLANIDE
CCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
ANEIIADGSDAEMVEMAKMQLDEAKDRLPELEEEIKFMLIPKDPEDAKNVMVEIRAGTGG
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCEEEEEECCCCC
DEASIFAGDLFRMYTKYCETMGWRTSVVDMNEGTSGGFKEVIFEVTGEDVYGTLKFEAGV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEHHHH
HRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQVDMNDVRVDFFCSSGPGGQSVNTTKS
HHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCEEEEEEEECCCCCCCCCCCHHH
AVRLTHIPTGLVAQCQDEKSQHKNKDKALMVLRSRLYEMELAKKQEEDAKKRSSQVSSGD
HEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSAKIRTYNYAQGRVTDHRVGLTLYDLGNIMNGDIQKIVSELQLVNNMEKLKEASEVY
CCCEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA