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The map label for this gene is mdh

Identifier: 146300011

GI number: 146300011

Start: 2717825

End: 2718760

Strand: Direct

Name: mdh

Synonym: Fjoh_2255

Alternate gene names: 146300011

Gene position: 2717825-2718760 (Clockwise)

Preceding gene: 146300010

Following gene: 146300012

Centisome position: 44.58

GC content: 37.71

Gene sequence:

>936_bases
ATGAAAGTTACCATTGTAGGAGCAGGAAATGTTGGAGCTACTTGCGCAGATGTTATTTCTTATAGAGGAATTGCAAGCGA
GGTTGTATTGTTGGATATTAAAGAAGGTTTTGCCGAAGGAAAAGCGTTGGATATTACACAATGTGCGACAAATACTGGTT
TTAATACAAAAGTGTCTGGCGTTACCAACGACTATTCTAAAACCGCTGGAAGTGATGTAGTGGTTATTACATCAGGAATT
CCAAGAAAACCAGGAATGACTAGAGAAGAATTAATTGGTATTAATGCAGGAATTGTGAAAACAGTTGCTGAAAATGTACT
GAAATATTCTCCAAATACTATAATTGTTGTAGTTTCTAACCCAATGGATACTATGACATATTTAGCTTTAAAAGCTACAG
GACTGCCAAAAAACAGAATTATAGGTATGGGCGGGGCATTAGACAGCTCTCGTTTTAGAACTTATTTATCTTTGGCTTTA
GATAAACCTGCAAATGATATTTCTGCGATGGTAATTGGAGGTCATGGTGATACAACCATGATTCCGTTAACACGTTTGGC
TTCTTATAATGGAATTCCGGTTTCTCAGTTCCTTTCAGAAGATGTACTGCAAAAAGTAGCAGCAGATACTATGGTTGGCG
GTGCTACGCTTACAGGTCTTCTAGGGACTTCTGCGTGGTATGCTCCGGGAGCTTCTGTTGCTTATTTAGTAGACAGTATC
CTAAACGATCAAAAGAAAATGATTGCCTGCTCAGTTTTCGTAGAAGGCGAGTACGGACAAAATGATATATGTATAGGTGT
ACCGTGTATAATTGGTAAAAACGGTGTAGAAGAAATTGTAAATATCAATTTAAATGACCAGGAAAAGGCTTTATTTGCTA
AAAGCGCAGATGCGGTTCGTGGTATGAATGATGCTCTAAAATCGATTTTAGTGTAA

Upstream 100 bases:

>100_bases
AACTTTCAATTCGTTTAATTGTTTAAATTTACTTAAACGATTAAACGAATTGTTAATTTATAAAACAAACAAATTATTAA
TAACCGATAAAGTAAAAAAT

Downstream 100 bases:

>100_bases
TAAAAACAGGAAAAAAAGGAAAAGGCTGCACTTTTGCAGTCTTTTTTTTGAGATTAATTAATAAATAAATTGGTTAATCT
TTCCGTTAATTATATATTTG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVTIVGAGNVGATCADVISYRGIASEVVLLDIKEGFAEGKALDITQCATNTGFNTKVSGVTNDYSKTAGSDVVVITSGI
PRKPGMTREELIGINAGIVKTVAENVLKYSPNTIIVVVSNPMDTMTYLALKATGLPKNRIIGMGGALDSSRFRTYLSLAL
DKPANDISAMVIGGHGDTTMIPLTRLASYNGIPVSQFLSEDVLQKVAADTMVGGATLTGLLGTSAWYAPGASVAYLVDSI
LNDQKKMIACSVFVEGEYGQNDICIGVPCIIGKNGVEEIVNINLNDQEKALFAKSADAVRGMNDALKSILV

Sequences:

>Translated_311_residues
MKVTIVGAGNVGATCADVISYRGIASEVVLLDIKEGFAEGKALDITQCATNTGFNTKVSGVTNDYSKTAGSDVVVITSGI
PRKPGMTREELIGINAGIVKTVAENVLKYSPNTIIVVVSNPMDTMTYLALKATGLPKNRIIGMGGALDSSRFRTYLSLAL
DKPANDISAMVIGGHGDTTMIPLTRLASYNGIPVSQFLSEDVLQKVAADTMVGGATLTGLLGTSAWYAPGASVAYLVDSI
LNDQKKMIACSVFVEGEYGQNDICIGVPCIIGKNGVEEIVNINLNDQEKALFAKSADAVRGMNDALKSILV
>Mature_311_residues
MKVTIVGAGNVGATCADVISYRGIASEVVLLDIKEGFAEGKALDITQCATNTGFNTKVSGVTNDYSKTAGSDVVVITSGI
PRKPGMTREELIGINAGIVKTVAENVLKYSPNTIIVVVSNPMDTMTYLALKATGLPKNRIIGMGGALDSSRFRTYLSLAL
DKPANDISAMVIGGHGDTTMIPLTRLASYNGIPVSQFLSEDVLQKVAADTMVGGATLTGLLGTSAWYAPGASVAYLVDSI
LNDQKKMIACSVFVEGEYGQNDICIGVPCIIGKNGVEEIVNINLNDQEKALFAKSADAVRGMNDALKSILV

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=33.968253968254, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=33.968253968254, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI5031857, Length=314, Percent_Identity=34.0764331210191, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI260099723, Length=314, Percent_Identity=34.0764331210191, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI15082234, Length=314, Percent_Identity=31.8471337579618, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=33.5463258785943, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=33.5463258785943, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI291575128, Length=321, Percent_Identity=32.7102803738318, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI4557032, Length=321, Percent_Identity=32.7102803738318, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI260099727, Length=179, Percent_Identity=39.6648044692737, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI260099725, Length=179, Percent_Identity=39.6648044692737, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI103472011, Length=309, Percent_Identity=29.126213592233, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=31.6582914572864, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21735621, Length=297, Percent_Identity=31.3131313131313, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI103472015, Length=176, Percent_Identity=30.6818181818182, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789632, Length=321, Percent_Identity=30.8411214953271, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17535107, Length=299, Percent_Identity=31.1036789297659, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17554310, Length=304, Percent_Identity=29.9342105263158, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17561064, Length=169, Percent_Identity=29.585798816568, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566798, Length=169, Percent_Identity=29.585798816568, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322765, Length=298, Percent_Identity=29.5302013422819, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320125, Length=275, Percent_Identity=31.6363636363636, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=245, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17136226, Length=317, Percent_Identity=34.0694006309148, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=27.3615635179153, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647881, Length=316, Percent_Identity=32.2784810126582, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24663599, Length=322, Percent_Identity=27.0186335403727, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24663595, Length=290, Percent_Identity=26.8965517241379, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_FLAJ1 (A5FHP3)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194602.1
- ProteinModelPortal:   A5FHP3
- SMR:   A5FHP3
- STRING:   A5FHP3
- GeneID:   5092261
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2255
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   LDKPAND
- ProtClustDB:   PRK06223
- BioCyc:   FJOH376686:FJOH_2255-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 32562; Mature: 32562

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: ACT_SITE 175-175 BINDING 32-32 BINDING 82-82 BINDING 88-88 BINDING 95-95 BINDING 120-120 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTIVGAGNVGATCADVISYRGIASEVVLLDIKEGFAEGKALDITQCATNTGFNTKVSG
CEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCCCEEECC
VTNDYSKTAGSDVVVITSGIPRKPGMTREELIGINAGIVKTVAENVLKYSPNTIIVVVSN
CCCCHHHCCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECC
PMDTMTYLALKATGLPKNRIIGMGGALDSSRFRTYLSLALDKPANDISAMVIGGHGDTTM
CCHHHHEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEE
IPLTRLASYNGIPVSQFLSEDVLQKVAADTMVGGATLTGLLGTSAWYAPGASVAYLVDSI
EEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHH
LNDQKKMIACSVFVEGEYGQNDICIGVPCIIGKNGVEEIVNINLNDQEKALFAKSADAVR
HCCCHHEEEEEEEEECCCCCCCEEEECCEEECCCCHHHHEECCCCCHHHHHHHHCHHHHC
GMNDALKSILV
CHHHHHHHHCC
>Mature Secondary Structure
MKVTIVGAGNVGATCADVISYRGIASEVVLLDIKEGFAEGKALDITQCATNTGFNTKVSG
CEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCCCEEECC
VTNDYSKTAGSDVVVITSGIPRKPGMTREELIGINAGIVKTVAENVLKYSPNTIIVVVSN
CCCCHHHCCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECC
PMDTMTYLALKATGLPKNRIIGMGGALDSSRFRTYLSLALDKPANDISAMVIGGHGDTTM
CCHHHHEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEE
IPLTRLASYNGIPVSQFLSEDVLQKVAADTMVGGATLTGLLGTSAWYAPGASVAYLVDSI
EEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHH
LNDQKKMIACSVFVEGEYGQNDICIGVPCIIGKNGVEEIVNINLNDQEKALFAKSADAVR
HCCCHHEEEEEEEEECCCCCCCEEEECCEEECCCCHHHHEECCCCCHHHHHHHHCHHHHC
GMNDALKSILV
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA