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The map label for this gene is gyrB [H]

Identifier: 146300010

GI number: 146300010

Start: 2715776

End: 2717716

Strand: Direct

Name: gyrB [H]

Synonym: Fjoh_2254

Alternate gene names: 146300010

Gene position: 2715776-2717716 (Clockwise)

Preceding gene: 146300009

Following gene: 146300011

Centisome position: 44.54

GC content: 38.95

Gene sequence:

>1941_bases
ATGAGCGAAGAAATCAAGAAGAACAATTATTCAGCAGATAGTATTCAGGCATTAGAAGGAATGGAGCACGTAAGAATGCG
TCCATCGATGTATATTGGAGATGTAGGAGTTCGAGGGCTTCACCATTTGGTTTACGAGGTTGTTGATAACTCTATTGATG
AGGCGATGGGAGGACATTGTGATACTATTGGTGTTACAATAAACGAAGACGGTTCGGTTACAGTTGAAGATAACGGTCGT
GGTATTCCAGTTGATTTACATAAAAAAGAAGGAGTTTCAGCACTTGAGGTTGTAATGACTAAAATTGGTGCCGGAGGTAA
ATTCGATAAAGATTCGTATAAAGTTTCCGGAGGACTTCACGGAGTTGGGGTTTCTGTAGTAAATGCACTTTCGGTTCACA
TGAAATCGACTGTATTTAGAGAAGGAAAAATCTATGAGCAGGAATACGAAAGAGGAAAATCATTATATCCGGTAAAACAA
ATTGGAGAAACAGATAAAAGAGGTACACGTCAGACCTTTTTTCCAGATGATACTATTTTCCAGCAGACTACAGAGTTCTC
TTATGATACTTTATCAGCTCGTATGCGTGAGCTTTCTTTCTTAAATAAAGGAATTACGATCACGTTTACAGATAAAAGAG
AAGTAGATGACAAAGGCGAATTTAAAAGCGAAGTTTTTCATTCAGATGAAGGTCTTAAAGAATATATCCGCTATTTAGAT
GGAAACCGTGAGCCAATTATTTCTCACGTAATCAGCATGGATCACGAAAAAGGTGAAATCCCGGTTGAGGTTGCCTTAAT
CTATAATACAAGTTATTCTGAGAATATTTTCTCTTACGTAAATAACATTAATACACACGAAGGAGGTACGCACCTGCAAG
GTTTTAGAAGTGGTTTAACAAGAACACTTAAAAAATACGCTGATGCTTCTGGATTGTTAGATAAATTGAAATTTGAAATT
TCGGGAGATGACTTCCGTGAAGGTTTAACAGCTATTATTTCGGTAAAAGTTTCTGAGCCTCAGTTCGAAGGACAAACAAA
AACGAAACTTGGAAACAGAGAGGTTGTATCTCCGGTTTCTCAGGCAGTTGGAGAAATGCTTGAAAATTATTTGGAAGAAA
ATCCAAATGATGCTAAGATAATTATTCAAAAAGTAATCTTAGCAGCTCAGGCTCGTCACGCGGCGAAAAAAGCACGTGAA
ATGGTACAGCGTAAAACCGTTATGGGCGGCGGCGGATTACCAGGAAAATTATCAGATTGTTCTGAGCAGGATCCGGCAAG
ATGTGAGGTTTACTTAGTCGAGGGAGATTCGGCTGGTGGAACAGCAAAACAAGGACGTGACCGTAACTTTCAGGCGATTT
TACCATTACGTGGTAAGATTTTGAACGTTGAAAAAGCGATGCACCATAAAGTGTTCGAAAACGAAGAGATTCGTAATATC
TTTACTGCTTTAGGAGTTACCGTTGGTACAGCAGAAGACAGTAAAGCTTTGAATATTGAAAAACTTCGTTACCACAAAGT
AATCATCATGTGTGATGCCGATGTCGATGGAAGCCACATTTCTACCTTAATATTAACGTTCTTCTTCCGTTTCATGAAAG
AGCTTATCGAAGAAGGTCACGTTTATATCGCAGCACCGCCTTTATACTTGGTTAAAAAAGGAAACAAAAAAGAATACGCA
TGGAATGACGTTCAACGCGATCAGGCCAATGAAAGAATGGGAGGAAGCGCAAACATTCAGCGTTATAAAGGTCTTGGAGA
GATGAACGCAGAACAATTGTGGGAAACAACAATGGATCCTCAATTTAGAACTTTGCGTCAGGTAACAATCGATAGTCTTG
CAGAAGCAGATAGAGTTTTCTCTATGTTAATGGGAGATGAGGTACCGCCTCGTAGAGAATTTATCGAGAAAAATGCAGTT
TACGCAAATATCGATGCCTAA

Upstream 100 bases:

>100_bases
AATAACTTAAGTGCTTTAAGTCGGAATTTTATGGGTATATACCCCGCGTTTTTATATATTTGCGTGTTAGACAGTAAATA
CATTTTTTAGAATAAATTAT

Downstream 100 bases:

>100_bases
TAAAAAATAACTTTCAATTCGTTTAATTGTTTAAATTTACTTAAACGATTAAACGAATTGTTAATTTATAAAACAAACAA
ATTATTAATAACCGATAAAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MSEEIKKNNYSADSIQALEGMEHVRMRPSMYIGDVGVRGLHHLVYEVVDNSIDEAMGGHCDTIGVTINEDGSVTVEDNGR
GIPVDLHKKEGVSALEVVMTKIGAGGKFDKDSYKVSGGLHGVGVSVVNALSVHMKSTVFREGKIYEQEYERGKSLYPVKQ
IGETDKRGTRQTFFPDDTIFQQTTEFSYDTLSARMRELSFLNKGITITFTDKREVDDKGEFKSEVFHSDEGLKEYIRYLD
GNREPIISHVISMDHEKGEIPVEVALIYNTSYSENIFSYVNNINTHEGGTHLQGFRSGLTRTLKKYADASGLLDKLKFEI
SGDDFREGLTAIISVKVSEPQFEGQTKTKLGNREVVSPVSQAVGEMLENYLEENPNDAKIIIQKVILAAQARHAAKKARE
MVQRKTVMGGGGLPGKLSDCSEQDPARCEVYLVEGDSAGGTAKQGRDRNFQAILPLRGKILNVEKAMHHKVFENEEIRNI
FTALGVTVGTAEDSKALNIEKLRYHKVIIMCDADVDGSHISTLILTFFFRFMKELIEEGHVYIAAPPLYLVKKGNKKEYA
WNDVQRDQANERMGGSANIQRYKGLGEMNAEQLWETTMDPQFRTLRQVTIDSLAEADRVFSMLMGDEVPPRREFIEKNAV
YANIDA

Sequences:

>Translated_646_residues
MSEEIKKNNYSADSIQALEGMEHVRMRPSMYIGDVGVRGLHHLVYEVVDNSIDEAMGGHCDTIGVTINEDGSVTVEDNGR
GIPVDLHKKEGVSALEVVMTKIGAGGKFDKDSYKVSGGLHGVGVSVVNALSVHMKSTVFREGKIYEQEYERGKSLYPVKQ
IGETDKRGTRQTFFPDDTIFQQTTEFSYDTLSARMRELSFLNKGITITFTDKREVDDKGEFKSEVFHSDEGLKEYIRYLD
GNREPIISHVISMDHEKGEIPVEVALIYNTSYSENIFSYVNNINTHEGGTHLQGFRSGLTRTLKKYADASGLLDKLKFEI
SGDDFREGLTAIISVKVSEPQFEGQTKTKLGNREVVSPVSQAVGEMLENYLEENPNDAKIIIQKVILAAQARHAAKKARE
MVQRKTVMGGGGLPGKLSDCSEQDPARCEVYLVEGDSAGGTAKQGRDRNFQAILPLRGKILNVEKAMHHKVFENEEIRNI
FTALGVTVGTAEDSKALNIEKLRYHKVIIMCDADVDGSHISTLILTFFFRFMKELIEEGHVYIAAPPLYLVKKGNKKEYA
WNDVQRDQANERMGGSANIQRYKGLGEMNAEQLWETTMDPQFRTLRQVTIDSLAEADRVFSMLMGDEVPPRREFIEKNAV
YANIDA
>Mature_645_residues
SEEIKKNNYSADSIQALEGMEHVRMRPSMYIGDVGVRGLHHLVYEVVDNSIDEAMGGHCDTIGVTINEDGSVTVEDNGRG
IPVDLHKKEGVSALEVVMTKIGAGGKFDKDSYKVSGGLHGVGVSVVNALSVHMKSTVFREGKIYEQEYERGKSLYPVKQI
GETDKRGTRQTFFPDDTIFQQTTEFSYDTLSARMRELSFLNKGITITFTDKREVDDKGEFKSEVFHSDEGLKEYIRYLDG
NREPIISHVISMDHEKGEIPVEVALIYNTSYSENIFSYVNNINTHEGGTHLQGFRSGLTRTLKKYADASGLLDKLKFEIS
GDDFREGLTAIISVKVSEPQFEGQTKTKLGNREVVSPVSQAVGEMLENYLEENPNDAKIIIQKVILAAQARHAAKKAREM
VQRKTVMGGGGLPGKLSDCSEQDPARCEVYLVEGDSAGGTAKQGRDRNFQAILPLRGKILNVEKAMHHKVFENEEIRNIF
TALGVTVGTAEDSKALNIEKLRYHKVIIMCDADVDGSHISTLILTFFFRFMKELIEEGHVYIAAPPLYLVKKGNKKEYAW
NDVQRDQANERMGGSANIQRYKGLGEMNAEQLWETTMDPQFRTLRQVTIDSLAEADRVFSMLMGDEVPPRREFIEKNAVY
ANIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=627, Percent_Identity=25.9968102073365, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI19913406, Length=628, Percent_Identity=25.4777070063694, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI48994957, Length=562, Percent_Identity=54.8042704626335, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1789408, Length=647, Percent_Identity=38.4853168469861, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI212645845, Length=557, Percent_Identity=27.2890484739677, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17535065, Length=633, Percent_Identity=24.0126382306477, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI212645657, Length=191, Percent_Identity=28.2722513089005, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=687, Percent_Identity=24.8908296943231, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17136538, Length=625, Percent_Identity=26.08, Blast_Score=142, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72287; Mature: 72156

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEIKKNNYSADSIQALEGMEHVRMRPSMYIGDVGVRGLHHLVYEVVDNSIDEAMGGHC
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
DTIGVTINEDGSVTVEDNGRGIPVDLHKKEGVSALEVVMTKIGAGGKFDKDSYKVSGGLH
CEEEEEECCCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCC
GVGVSVVNALSVHMKSTVFREGKIYEQEYERGKSLYPVKQIGETDKRGTRQTFFPDDTIF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHH
QQTTEFSYDTLSARMRELSFLNKGITITFTDKREVDDKGEFKSEVFHSDEGLKEYIRYLD
HHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHC
GNREPIISHVISMDHEKGEIPVEVALIYNTSYSENIFSYVNNINTHEGGTHLQGFRSGLT
CCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHH
RTLKKYADASGLLDKLKFEISGDDFREGLTAIISVKVSEPQFEGQTKTKLGNREVVSPVS
HHHHHHHHHHHHHHHHEEEECCHHHHHHHEEEEEEEECCCCCCCCHHHCCCCHHHHHHHH
QAVGEMLENYLEENPNDAKIIIQKVILAAQARHAAKKAREMVQRKTVMGGGGLPGKLSDC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SEQDPARCEVYLVEGDSAGGTAKQGRDRNFQAILPLRGKILNVEKAMHHKVFENEEIRNI
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHCCCHHHHHH
FTALGVTVGTAEDSKALNIEKLRYHKVIIMCDADVDGSHISTLILTFFFRFMKELIEEGH
HHHHCCEEECCCCCCCCCHHEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VYIAAPPLYLVKKGNKKEYAWNDVQRDQANERMGGSANIQRYKGLGEMNAEQLWETTMDP
EEEEECCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHCCCH
QFRTLRQVTIDSLAEADRVFSMLMGDEVPPRREFIEKNAVYANIDA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCC
>Mature Secondary Structure 
SEEIKKNNYSADSIQALEGMEHVRMRPSMYIGDVGVRGLHHLVYEVVDNSIDEAMGGHC
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
DTIGVTINEDGSVTVEDNGRGIPVDLHKKEGVSALEVVMTKIGAGGKFDKDSYKVSGGLH
CEEEEEECCCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCC
GVGVSVVNALSVHMKSTVFREGKIYEQEYERGKSLYPVKQIGETDKRGTRQTFFPDDTIF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHH
QQTTEFSYDTLSARMRELSFLNKGITITFTDKREVDDKGEFKSEVFHSDEGLKEYIRYLD
HHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHC
GNREPIISHVISMDHEKGEIPVEVALIYNTSYSENIFSYVNNINTHEGGTHLQGFRSGLT
CCCCHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHH
RTLKKYADASGLLDKLKFEISGDDFREGLTAIISVKVSEPQFEGQTKTKLGNREVVSPVS
HHHHHHHHHHHHHHHHEEEECCHHHHHHHEEEEEEEECCCCCCCCHHHCCCCHHHHHHHH
QAVGEMLENYLEENPNDAKIIIQKVILAAQARHAAKKAREMVQRKTVMGGGGLPGKLSDC
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SEQDPARCEVYLVEGDSAGGTAKQGRDRNFQAILPLRGKILNVEKAMHHKVFENEEIRNI
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHCCCHHHHHH
FTALGVTVGTAEDSKALNIEKLRYHKVIIMCDADVDGSHISTLILTFFFRFMKELIEEGH
HHHHCCEEECCCCCCCCCHHEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VYIAAPPLYLVKKGNKKEYAWNDVQRDQANERMGGSANIQRYKGLGEMNAEQLWETTMDP
EEEEECCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHCCCH
QFRTLRQVTIDSLAEADRVFSMLMGDEVPPRREFIEKNAVYANIDA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]