Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 146300007

GI number: 146300007

Start: 2708930

End: 2710606

Strand: Direct

Name: asnB [H]

Synonym: Fjoh_2251

Alternate gene names: 146300007

Gene position: 2708930-2710606 (Clockwise)

Preceding gene: 146300006

Following gene: 146300008

Centisome position: 44.43

GC content: 37.69

Gene sequence:

>1677_bases
ATGTGTGGAATTGTATGTGCCTTTGATCTAAAGCAAAAAGCCGAGAGTCTAAGACCTCAAGTATTAGAAATGTCTAAAAT
CATTCGTCACCGCGGACCAGACTGGAGCGGAATTTACAGTAATGATAAAGCAATTCTTTCTCACGAGCGTCTGGCAATTG
TAGATCCAGCTTCAGGAAAACAACCTTTATTTACAGAAGATAAAAAATTGGTTTTGGCTGCAAACGGTGAAATTTACAAT
CACAGAGATTTGCGTAAACAATTTGAAGGAAAATATAACTTTCAGACTGAAAGTGACTGCGAAGTTATTTTAGCATTATA
CAGAGAAAAAGGAGTAAACTTTGTTGATGAATTAAACGGAATCTTTGGTTTTGCAATTTATGATGTAGATAAAGATGAGT
ATTTCGTAGCTCGTGACCACATGGGAATTATTCCATTATATATTGGATGGGATCAGCACGGAACTTTCTATGTAGCTTCT
GAGTTAAAAGCTTTAGAAGGATATTGCACAAAAATTGAATTATTCCCTCCAGGACATTATTTATCAAGCAAAGACGGAGA
GTTTGTACAATGGTACAAAAGAGACTGGACAGAATATGATGCAGTAAAAGACAATGAAACAAGCATTCCAGAAATTAGAA
AAGCATTAGAAGCAGCGGTTCACAGACAATTAATGAGTGACGTTCCTTACGGAGTTTTACTTTCTGGAGGTTTAGATTCT
TCTATCACTTCGGCTGTAGCCAAAAAATTTGCACAAAAAAGAATTGAGTCAGATGATACCACAGATGCCTGGTATCCGCA
ATTGCACTCTTTCTCAGTTGGATTAGAAGGTTCTCCGGATTTAGCTGCAGCTAGAAAAGTAGCAGATCATATTGGAACTA
TTCACCACGAAATTAAATTCACAATCCAAGAAGGTTTAGATGCCGTTCGTGATGTAATTTACAACCTTGAAACGTATGAT
GTAACAACAGTAAGAGCTTCAACTCCAATGTGGTTAATGGCTAGAGTTATCAAATCTATGGGAATCAAAATGGTTCTTTC
CGGAGAAGGAGCAGATGAGCTATTTGGAGGATATTTATATTTCCACAAAGCACCAAACGCTAAAGAGTTCCACGAAGAAA
ACGTTCGTAAATTAGGAAAACTTCACATGTACGATTGTTTACGTGCAAACAAAAGTTTAGCAGCGTGGGGAATCGAAGGC
CGTGTACCATTCTTAGATAAAGAATTTATGGATGTTGCAATGCGCATCAACCCACAAGATAAAATGATCAACAAAGAACA
TCCAATGGAAAAATGGGTTGTTCGTAAAGCTTTTGAAGATATGCTTCCTGAAAGTGTGGCATGGAGACAAAAAGAGCAGT
TTTCTGATGGAGTAGGATACAGCTGGATCGATACTTTAAAAGAAGTTGTGGCAAGAGAAGTTTCAGACGAGCAATTAGCA
AACGCAAGATTCAAATTTCCATTACAGACTCCAACTTCAAAAGAAGAATATTACTATCGTTCTATCTTCTCAGAACATTT
CCCAAGCGATGCTGCAGCATTATGCGTTCCGCAGGAAGCAAGTGTTGCCTGCAGTACAAAAATTGCTTTAGAGTGGGATG
AAGCGTTTAAAAACTTAAACGATCCTTCAGGAAGAGCAGTTGCAAATGTGCATGACGATGCTTATGTAAAAGCATAA

Upstream 100 bases:

>100_bases
TAAATTGTAATTGAAATTGGTGTTTGTGTTTTTCAATTGTAATCGATGCCGCATCTTTGCCCTATCAAAATGAAATTTAA
AGTTTTAAAAATATAAAATT

Downstream 100 bases:

>100_bases
ATGAGTTAATTATTAGAATTAGTATATATATTGCTGGAAAAACGTTCTCTACCGAGGGCGTTTTTCTTGTCTTAAATTGT
TAAATTCTATATTTTCAGTT

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MCGIVCAFDLKQKAESLRPQVLEMSKIIRHRGPDWSGIYSNDKAILSHERLAIVDPASGKQPLFTEDKKLVLAANGEIYN
HRDLRKQFEGKYNFQTESDCEVILALYREKGVNFVDELNGIFGFAIYDVDKDEYFVARDHMGIIPLYIGWDQHGTFYVAS
ELKALEGYCTKIELFPPGHYLSSKDGEFVQWYKRDWTEYDAVKDNETSIPEIRKALEAAVHRQLMSDVPYGVLLSGGLDS
SITSAVAKKFAQKRIESDDTTDAWYPQLHSFSVGLEGSPDLAAARKVADHIGTIHHEIKFTIQEGLDAVRDVIYNLETYD
VTTVRASTPMWLMARVIKSMGIKMVLSGEGADELFGGYLYFHKAPNAKEFHEENVRKLGKLHMYDCLRANKSLAAWGIEG
RVPFLDKEFMDVAMRINPQDKMINKEHPMEKWVVRKAFEDMLPESVAWRQKEQFSDGVGYSWIDTLKEVVAREVSDEQLA
NARFKFPLQTPTSKEEYYYRSIFSEHFPSDAAALCVPQEASVACSTKIALEWDEAFKNLNDPSGRAVANVHDDAYVKA

Sequences:

>Translated_558_residues
MCGIVCAFDLKQKAESLRPQVLEMSKIIRHRGPDWSGIYSNDKAILSHERLAIVDPASGKQPLFTEDKKLVLAANGEIYN
HRDLRKQFEGKYNFQTESDCEVILALYREKGVNFVDELNGIFGFAIYDVDKDEYFVARDHMGIIPLYIGWDQHGTFYVAS
ELKALEGYCTKIELFPPGHYLSSKDGEFVQWYKRDWTEYDAVKDNETSIPEIRKALEAAVHRQLMSDVPYGVLLSGGLDS
SITSAVAKKFAQKRIESDDTTDAWYPQLHSFSVGLEGSPDLAAARKVADHIGTIHHEIKFTIQEGLDAVRDVIYNLETYD
VTTVRASTPMWLMARVIKSMGIKMVLSGEGADELFGGYLYFHKAPNAKEFHEENVRKLGKLHMYDCLRANKSLAAWGIEG
RVPFLDKEFMDVAMRINPQDKMINKEHPMEKWVVRKAFEDMLPESVAWRQKEQFSDGVGYSWIDTLKEVVAREVSDEQLA
NARFKFPLQTPTSKEEYYYRSIFSEHFPSDAAALCVPQEASVACSTKIALEWDEAFKNLNDPSGRAVANVHDDAYVKA
>Mature_558_residues
MCGIVCAFDLKQKAESLRPQVLEMSKIIRHRGPDWSGIYSNDKAILSHERLAIVDPASGKQPLFTEDKKLVLAANGEIYN
HRDLRKQFEGKYNFQTESDCEVILALYREKGVNFVDELNGIFGFAIYDVDKDEYFVARDHMGIIPLYIGWDQHGTFYVAS
ELKALEGYCTKIELFPPGHYLSSKDGEFVQWYKRDWTEYDAVKDNETSIPEIRKALEAAVHRQLMSDVPYGVLLSGGLDS
SITSAVAKKFAQKRIESDDTTDAWYPQLHSFSVGLEGSPDLAAARKVADHIGTIHHEIKFTIQEGLDAVRDVIYNLETYD
VTTVRASTPMWLMARVIKSMGIKMVLSGEGADELFGGYLYFHKAPNAKEFHEENVRKLGKLHMYDCLRANKSLAAWGIEG
RVPFLDKEFMDVAMRINPQDKMINKEHPMEKWVVRKAFEDMLPESVAWRQKEQFSDGVGYSWIDTLKEVVAREVSDEQLA
NARFKFPLQTPTSKEEYYYRSIFSEHFPSDAAALCVPQEASVACSTKIALEWDEAFKNLNDPSGRAVANVHDDAYVKA

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=594, Percent_Identity=38.047138047138, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI168229252, Length=594, Percent_Identity=38.047138047138, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI168229250, Length=594, Percent_Identity=38.047138047138, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI296010848, Length=567, Percent_Identity=38.2716049382716, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI296010852, Length=507, Percent_Identity=38.0670611439842, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI296010850, Length=507, Percent_Identity=38.0670611439842, Blast_Score=295, Evalue=6e-80,
Organism=Escherichia coli, GI1786889, Length=555, Percent_Identity=66.6666666666667, Blast_Score=788, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=569, Percent_Identity=43.2337434094903, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=43.2915921288014, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17560178, Length=524, Percent_Identity=34.9236641221374, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI25147560, Length=347, Percent_Identity=37.463976945245, Blast_Score=209, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321563, Length=581, Percent_Identity=47.6764199655766, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325403, Length=582, Percent_Identity=46.3917525773196, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI45553209, Length=574, Percent_Identity=46.3414634146341, Blast_Score=476, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 63444; Mature: 63444

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVCAFDLKQKAESLRPQVLEMSKIIRHRGPDWSGIYSNDKAILSHERLAIVDPASGK
CCCEEEEHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEECCCCCC
QPLFTEDKKLVLAANGEIYNHRDLRKQFEGKYNFQTESDCEVILALYREKGVNFVDELNG
CCCEECCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC
IFGFAIYDVDKDEYFVARDHMGIIPLYIGWDQHGTFYVASELKALEGYCTKIELFPPGHY
CEEEEEEECCCCCEEEEECCCCEEEEEEEECCCCCEEEHHHHHHHCCCEEEEEEECCCCC
LSSKDGEFVQWYKRDWTEYDAVKDNETSIPEIRKALEAAVHRQLMSDVPYGVLLSGGLDS
CCCCCCHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAVAKKFAQKRIESDDTTDAWYPQLHSFSVGLEGSPDLAAARKVADHIGTIHHEIKF
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAVRDVIYNLETYDVTTVRASTPMWLMARVIKSMGIKMVLSGEGADELFGGYLY
HHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAKEFHEENVRKLGKLHMYDCLRANKSLAAWGIEGRVPFLDKEFMDVAMRINPQD
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHEECCHH
KMINKEHPMEKWVVRKAFEDMLPESVAWRQKEQFSDGVGYSWIDTLKEVVAREVSDEQLA
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
NARFKFPLQTPTSKEEYYYRSIFSEHFPSDAAALCVPQEASVACSTKIALEWDEAFKNLN
CCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEHHHHHCCCC
DPSGRAVANVHDDAYVKA
CCCCCEEEEECCCCEECC
>Mature Secondary Structure
MCGIVCAFDLKQKAESLRPQVLEMSKIIRHRGPDWSGIYSNDKAILSHERLAIVDPASGK
CCCEEEEHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEECCCCCC
QPLFTEDKKLVLAANGEIYNHRDLRKQFEGKYNFQTESDCEVILALYREKGVNFVDELNG
CCCEECCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCC
IFGFAIYDVDKDEYFVARDHMGIIPLYIGWDQHGTFYVASELKALEGYCTKIELFPPGHY
CEEEEEEECCCCCEEEEECCCCEEEEEEEECCCCCEEEHHHHHHHCCCEEEEEEECCCCC
LSSKDGEFVQWYKRDWTEYDAVKDNETSIPEIRKALEAAVHRQLMSDVPYGVLLSGGLDS
CCCCCCHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SITSAVAKKFAQKRIESDDTTDAWYPQLHSFSVGLEGSPDLAAARKVADHIGTIHHEIKF
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TIQEGLDAVRDVIYNLETYDVTTVRASTPMWLMARVIKSMGIKMVLSGEGADELFGGYLY
HHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAKEFHEENVRKLGKLHMYDCLRANKSLAAWGIEGRVPFLDKEFMDVAMRINPQD
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHEECCHH
KMINKEHPMEKWVVRKAFEDMLPESVAWRQKEQFSDGVGYSWIDTLKEVVAREVSDEQLA
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
NARFKFPLQTPTSKEEYYYRSIFSEHFPSDAAALCVPQEASVACSTKIALEWDEAFKNLN
CCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEHHHHHCCCC
DPSGRAVANVHDDAYVKA
CCCCCEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]