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The map label for this gene is asnB [H]

Identifier: 146300008

GI number: 146300008

Start: 2710921

End: 2712534

Strand: Direct

Name: asnB [H]

Synonym: Fjoh_2252

Alternate gene names: 146300008

Gene position: 2710921-2712534 (Clockwise)

Preceding gene: 146300007

Following gene: 146300009

Centisome position: 44.46

GC content: 36.25

Gene sequence:

>1614_bases
ATGTCTGGATTATTGGCCGTTATTGGTAAAGGAAAAGACCCGCAGCTTGTAAAAGAACTTTCTAACAGAATGTCACACCG
TGGCCCGGATGAAAGCGATTTGCATATTATGGAAAATGGAAGTATACTTTGTCATGAAAGTCTGTCTATTATCGATCTGC
ATTCGGGAAAACAGCCTATTCAGGGAACGAACAAAGCATGGATGGTTCATGATGGTGAAATTTATAATTATCAGGAATTA
AAAAACACAATTTTAAAAGATCATACTTTTAGAACCAATTCAGATTCAGAAGTTATCGTGCATTTATATGAAGAGTTTGG
ATATGATTTTTGCAACAAACTTGACGGTGATTTTGCTTTCGTAATTATTGACGGTGATAATTACATTGCAGGAAGAGATC
CAATTGGAGTAAAACCTCTATATTATGGATTAGATGAAAGAGGGAGAATTTATTTTTCTTCAGAAATGAAATCAATTGCC
GATCAGTGTAAATCATTTTCAACTTTTCCTCCAGGGCATTATTATACAGCAAAAAGCGGTTTCGTAAAATATTATCATCC
GGAATACGAAGATCATTTAAAGGCTGATCAGCCGCTTGATTTTGGATTAATCCGCAAAAGTTTAATCGAAGCCACACGCA
AACGTTTAATAGGCGAAGTTCCTGTTGGAGTTGTATTATCCGGTGGTTTAGATACTTCTTTAATTTCTTCTGTGGCTTCA
AGACTGCTAAAAGAAAATGGTAAAAAACTGCCGTCTTTTTCTATCGGTTTAGATGCTGATGCCCCAGATAATATTTCGGC
TAGAAAAGCAGCAGAATTTTTAGGTACAGATCATCATGAAATACATTTTACTGTAAAAGAAGGAGTAGAAGCTTTAGAGC
AGGTTATTGCATCTATAGAAACCTATGATATTATTTCTGTTCGTTCAGGAGTTCCAATGTATTTATTGTCTAAAGCAATC
AGCGAAAAAGGCGTAAAAGTAATTTTGTCAGGAGAAGGCGCCGATGAAATTTTTGGAGGACATTTGTATTTTAGAAATGC
GCCTTCGACAGAAGAATTTCAGGACGAAACTATCGAAAGAGTCCAAAAACTATTTACAGCAGATTTACTTCGTGCGGATA
AAACAACAATGGCTAACGGAATCGAAGCCAGAGTTCCGTTTCTTGATAAAGATTTTTTAGATGTTACCATTCGAATTAAA
ACCGAAGAAAAACAACCTAAAACTTACGATGGAGTTGAAAAATATATCTTAAGAAAAGCATTTGATACTCCCGAAGATCC
TTATTTGCCTGCAGAAGTTTTGTGGAGACAAAAAGAGCAGTTTTCTGATGGAGTAGGATACAACTGGGTTGATGAGTTGA
TAGAGTATTGTGATTCGCAGGTTACAGATGAACAATTGGCTGGTGCAAGTGCAGAATTTCCATACAATACGCCAACAACA
AAAGAAGCGTATTATTACAGATCGATTTTTCATAAATATTATCCACAGGTCAGTGCGGCGCAAACCGTTCGTAAATGGAT
TCCAAAATGGCAGGAAAATCAAGATCCAAGCGGAAGAGCTAATGCAGCACACTTAAAAGGAAATTTTGAGAATGTGAAAT
CTGTAGCCGTTTAA

Upstream 100 bases:

>100_bases
TAATATTTAGATTTGCCTGCAATAGAAAATTTTAATGATTTTTTAATGTTCAGCTTATTTTAAGTTGACACAGAAGAATA
AAAATATAAAATAGATTAGT

Downstream 100 bases:

>100_bases
AAAGTATTTTAAAAGATAAATTCCAGACGGAATTTCAAAACATAAAACCGAAACAGATAATAGGATCTCGTTTCGGTTTT
TTTATGCGTGGAATTAAGAT

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSGLLAVIGKGKDPQLVKELSNRMSHRGPDESDLHIMENGSILCHESLSIIDLHSGKQPIQGTNKAWMVHDGEIYNYQEL
KNTILKDHTFRTNSDSEVIVHLYEEFGYDFCNKLDGDFAFVIIDGDNYIAGRDPIGVKPLYYGLDERGRIYFSSEMKSIA
DQCKSFSTFPPGHYYTAKSGFVKYYHPEYEDHLKADQPLDFGLIRKSLIEATRKRLIGEVPVGVVLSGGLDTSLISSVAS
RLLKENGKKLPSFSIGLDADAPDNISARKAAEFLGTDHHEIHFTVKEGVEALEQVIASIETYDIISVRSGVPMYLLSKAI
SEKGVKVILSGEGADEIFGGHLYFRNAPSTEEFQDETIERVQKLFTADLLRADKTTMANGIEARVPFLDKDFLDVTIRIK
TEEKQPKTYDGVEKYILRKAFDTPEDPYLPAEVLWRQKEQFSDGVGYNWVDELIEYCDSQVTDEQLAGASAEFPYNTPTT
KEAYYYRSIFHKYYPQVSAAQTVRKWIPKWQENQDPSGRANAAHLKGNFENVKSVAV

Sequences:

>Translated_537_residues
MSGLLAVIGKGKDPQLVKELSNRMSHRGPDESDLHIMENGSILCHESLSIIDLHSGKQPIQGTNKAWMVHDGEIYNYQEL
KNTILKDHTFRTNSDSEVIVHLYEEFGYDFCNKLDGDFAFVIIDGDNYIAGRDPIGVKPLYYGLDERGRIYFSSEMKSIA
DQCKSFSTFPPGHYYTAKSGFVKYYHPEYEDHLKADQPLDFGLIRKSLIEATRKRLIGEVPVGVVLSGGLDTSLISSVAS
RLLKENGKKLPSFSIGLDADAPDNISARKAAEFLGTDHHEIHFTVKEGVEALEQVIASIETYDIISVRSGVPMYLLSKAI
SEKGVKVILSGEGADEIFGGHLYFRNAPSTEEFQDETIERVQKLFTADLLRADKTTMANGIEARVPFLDKDFLDVTIRIK
TEEKQPKTYDGVEKYILRKAFDTPEDPYLPAEVLWRQKEQFSDGVGYNWVDELIEYCDSQVTDEQLAGASAEFPYNTPTT
KEAYYYRSIFHKYYPQVSAAQTVRKWIPKWQENQDPSGRANAAHLKGNFENVKSVAV
>Mature_536_residues
SGLLAVIGKGKDPQLVKELSNRMSHRGPDESDLHIMENGSILCHESLSIIDLHSGKQPIQGTNKAWMVHDGEIYNYQELK
NTILKDHTFRTNSDSEVIVHLYEEFGYDFCNKLDGDFAFVIIDGDNYIAGRDPIGVKPLYYGLDERGRIYFSSEMKSIAD
QCKSFSTFPPGHYYTAKSGFVKYYHPEYEDHLKADQPLDFGLIRKSLIEATRKRLIGEVPVGVVLSGGLDTSLISSVASR
LLKENGKKLPSFSIGLDADAPDNISARKAAEFLGTDHHEIHFTVKEGVEALEQVIASIETYDIISVRSGVPMYLLSKAIS
EKGVKVILSGEGADEIFGGHLYFRNAPSTEEFQDETIERVQKLFTADLLRADKTTMANGIEARVPFLDKDFLDVTIRIKT
EEKQPKTYDGVEKYILRKAFDTPEDPYLPAEVLWRQKEQFSDGVGYNWVDELIEYCDSQVTDEQLAGASAEFPYNTPTTK
EAYYYRSIFHKYYPQVSAAQTVRKWIPKWQENQDPSGRANAAHLKGNFENVKSVAV

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=561, Percent_Identity=37.4331550802139, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI168229252, Length=561, Percent_Identity=37.4331550802139, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI168229250, Length=561, Percent_Identity=37.4331550802139, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI296010848, Length=538, Percent_Identity=37.546468401487, Blast_Score=328, Evalue=9e-90,
Organism=Homo sapiens, GI296010852, Length=467, Percent_Identity=39.4004282655246, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI296010850, Length=467, Percent_Identity=39.4004282655246, Blast_Score=301, Evalue=8e-82,
Organism=Escherichia coli, GI1786889, Length=534, Percent_Identity=44.5692883895131, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25147557, Length=534, Percent_Identity=53.1835205992509, Blast_Score=585, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71993933, Length=511, Percent_Identity=53.6203522504892, Blast_Score=578, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI17560178, Length=555, Percent_Identity=35.6756756756757, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25147560, Length=310, Percent_Identity=46.7741935483871, Blast_Score=286, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6321563, Length=550, Percent_Identity=46.5454545454545, Blast_Score=471, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6325403, Length=551, Percent_Identity=45.5535390199637, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI45553209, Length=557, Percent_Identity=41.2926391382406, Blast_Score=427, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 60540; Mature: 60408

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLLAVIGKGKDPQLVKELSNRMSHRGPDESDLHIMENGSILCHESLSIIDLHSGKQPI
CCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCEEEEECCCCCCC
QGTNKAWMVHDGEIYNYQELKNTILKDHTFRTNSDSEVIVHLYEEFGYDFCNKLDGDFAF
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHCCCCEEE
VIIDGDNYIAGRDPIGVKPLYYGLDERGRIYFSSEMKSIADQCKSFSTFPPGHYYTAKSG
EEECCCCEECCCCCCCCCHHEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEECCCC
FVKYYHPEYEDHLKADQPLDFGLIRKSLIEATRKRLIGEVPVGVVLSGGLDTSLISSVAS
CEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHH
RLLKENGKKLPSFSIGLDADAPDNISARKAAEFLGTDHHEIHFTVKEGVEALEQVIASIE
HHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHH
TYDIISVRSGVPMYLLSKAISEKGVKVILSGEGADEIFGGHLYFRNAPSTEEFQDETIER
HHEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCEEEECCCCCCHHHHHHHHHH
VQKLFTADLLRADKTTMANGIEARVPFLDKDFLDVTIRIKTEEKQPKTYDGVEKYILRKA
HHHHHHHHHHHCCHHHHHCCCEEECCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHH
FDTPEDPYLPAEVLWRQKEQFSDGVGYNWVDELIEYCDSQVTDEQLAGASAEFPYNTPTT
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
KEAYYYRSIFHKYYPQVSAAQTVRKWIPKWQENQDPSGRANAAHLKGNFENVKSVAV
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHCC
>Mature Secondary Structure 
SGLLAVIGKGKDPQLVKELSNRMSHRGPDESDLHIMENGSILCHESLSIIDLHSGKQPI
CCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCEEEEECCCCCCC
QGTNKAWMVHDGEIYNYQELKNTILKDHTFRTNSDSEVIVHLYEEFGYDFCNKLDGDFAF
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHCCCCEEE
VIIDGDNYIAGRDPIGVKPLYYGLDERGRIYFSSEMKSIADQCKSFSTFPPGHYYTAKSG
EEECCCCEECCCCCCCCCHHEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEECCCC
FVKYYHPEYEDHLKADQPLDFGLIRKSLIEATRKRLIGEVPVGVVLSGGLDTSLISSVAS
CEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHH
RLLKENGKKLPSFSIGLDADAPDNISARKAAEFLGTDHHEIHFTVKEGVEALEQVIASIE
HHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHH
TYDIISVRSGVPMYLLSKAISEKGVKVILSGEGADEIFGGHLYFRNAPSTEEFQDETIER
HHEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCEEEECCCCCCHHHHHHHHHH
VQKLFTADLLRADKTTMANGIEARVPFLDKDFLDVTIRIKTEEKQPKTYDGVEKYILRKA
HHHHHHHHHHHCCHHHHHCCCEEECCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHH
FDTPEDPYLPAEVLWRQKEQFSDGVGYNWVDELIEYCDSQVTDEQLAGASAEFPYNTPTT
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
KEAYYYRSIFHKYYPQVSAAQTVRKWIPKWQENQDPSGRANAAHLKGNFENVKSVAV
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]