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The map label for this gene is odhL [H]

Identifier: 146299889

GI number: 146299889

Start: 2582567

End: 2583970

Strand: Direct

Name: odhL [H]

Synonym: Fjoh_2133

Alternate gene names: 146299889

Gene position: 2582567-2583970 (Clockwise)

Preceding gene: 146299888

Following gene: 146299890

Centisome position: 42.36

GC content: 38.03

Gene sequence:

>1404_bases
ATGAGTTCATTTGACGTAGTCATTATAGGTTCAGGTCCTGGAGGATATGTATCAGCAATTCGTTGCGCACAATTAGGTTT
CAAAACTGCAATTGTAGAAAAATATAATTCATTAGGCGGAACTTGCCTTAACGTAGGTTGTATTCCTTCAAAAGCATTAT
TATCTTCTTCTCATCATTATGCTGAAATTGCTCATTTTGCAGATCACGGAATCGAAGTTTCCGGCGATGTAAAAATCAAT
TTAGAGAAAATGATCGCACGCAAGCAAGCTGTTGTAGATCAAACCGTAGGTGGTATTAACTACTTAATGGAAAAAAATAA
AATTACTGTTTTCAATGGTTTAGGTTCATTCGTAGACGCAACTCACATTGCTGTTGCAAAAGCTGACGGAACATCAGAAA
CTATTGAAGCAAAATATACTGTAATTGCTACAGGATCAAAACCATCTTCTTTACCATTCATCAAAATTGATAAAGAAAGA
ATCATTACTTCTACTGAAGCTTTGGCTTTAAAAGAAGTTCCAAAACACTTAGTAATTATTGGTGGTGGAGTTATCGGAAT
CGAACTTGGACAAGTTTACCTTCGTTTAGGAGCTCAGGTTTCTGTAGTAGAATTCATGGACAGAATCATTCCAGGAATGG
ATAGTTCTTTATCTAAAGAATTGACTAAAGTATTGAAAAAACAAGGAATGAAATTCTACGTTTCTCACAAAGTAAAATCA
GTAGAAAGAAACGGCGATGCTGTTGTAGTTCAGGCAGAAAATGCAAAAGGAGAAACAATTACTCTTGAAGGAGATTATTC
ATTAGTTTCTGTTGGTCGTCGTCCTTACACAGACGGATTAAACGCTGACAAAGCTGGAGTAAAAATTTCAGACAGAGGAC
AAGTTGAAGTAAACGATCATTTACAAACTAGCGTTCCAAATATCTACGCAATTGGTGACGTTGTTCGCGGCGCAATGTTA
GCGCACAAAGCGGAAGAAGAAGGAGTAATGGTTGCTGAAATTTTAGCAGGTCAAAAACCACATATCGATTACAACTTAAT
TCCTGGTGTAGTTTACACTTGGCCAGAAGTTGCTGCAGTTGGACAAACTGAAGAGCAATTGAAAGCTGCAGGAGTAAAAT
ACAAATCTGGAAGTTTCCCATTCAAAGCGTTAGGACGTGCAAGAGCAAGTGCTGACTTAGACGGATTCGTAAAAATCCTT
GCTGACGAAAAAACAGACGAGGTTTTAGGAGTTCACATGATTGGTGCTCGTACAGCAGATTTAATTGCTGAAGCGGTTAC
TGCAATGGAATTTAAAGCTTCTGCTGAAGATATTTCTAGAATGAGCCACGCGCACCCAACTTTCGCGGAAGCGGTAAAAG
AAGCAGCATTAGCAGCTACAGAAAATAGAGCTTTACACGTATAA

Upstream 100 bases:

>100_bases
AACTAAAACCCAAAATTTCACGCAAAAAACATTGAAAATTAAACTTTAAACAGAACTTTATTGCTTAATTTTGTAACCGC
TAAAATAAAAACAAAATACT

Downstream 100 bases:

>100_bases
TTTTTACGTCGAATCATATTTAAAACCGTCAGAGCAGTCTGGCGGTTTTTTTATGCAATATTTTTAAGCGTTATATAATA
TAATAATCAAAAATAATTTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSSFDVVIIGSGPGGYVSAIRCAQLGFKTAIVEKYNSLGGTCLNVGCIPSKALLSSSHHYAEIAHFADHGIEVSGDVKIN
LEKMIARKQAVVDQTVGGINYLMEKNKITVFNGLGSFVDATHIAVAKADGTSETIEAKYTVIATGSKPSSLPFIKIDKER
IITSTEALALKEVPKHLVIIGGGVIGIELGQVYLRLGAQVSVVEFMDRIIPGMDSSLSKELTKVLKKQGMKFYVSHKVKS
VERNGDAVVVQAENAKGETITLEGDYSLVSVGRRPYTDGLNADKAGVKISDRGQVEVNDHLQTSVPNIYAIGDVVRGAML
AHKAEEEGVMVAEILAGQKPHIDYNLIPGVVYTWPEVAAVGQTEEQLKAAGVKYKSGSFPFKALGRARASADLDGFVKIL
ADEKTDEVLGVHMIGARTADLIAEAVTAMEFKASAEDISRMSHAHPTFAEAVKEAALAATENRALHV

Sequences:

>Translated_467_residues
MSSFDVVIIGSGPGGYVSAIRCAQLGFKTAIVEKYNSLGGTCLNVGCIPSKALLSSSHHYAEIAHFADHGIEVSGDVKIN
LEKMIARKQAVVDQTVGGINYLMEKNKITVFNGLGSFVDATHIAVAKADGTSETIEAKYTVIATGSKPSSLPFIKIDKER
IITSTEALALKEVPKHLVIIGGGVIGIELGQVYLRLGAQVSVVEFMDRIIPGMDSSLSKELTKVLKKQGMKFYVSHKVKS
VERNGDAVVVQAENAKGETITLEGDYSLVSVGRRPYTDGLNADKAGVKISDRGQVEVNDHLQTSVPNIYAIGDVVRGAML
AHKAEEEGVMVAEILAGQKPHIDYNLIPGVVYTWPEVAAVGQTEEQLKAAGVKYKSGSFPFKALGRARASADLDGFVKIL
ADEKTDEVLGVHMIGARTADLIAEAVTAMEFKASAEDISRMSHAHPTFAEAVKEAALAATENRALHV
>Mature_466_residues
SSFDVVIIGSGPGGYVSAIRCAQLGFKTAIVEKYNSLGGTCLNVGCIPSKALLSSSHHYAEIAHFADHGIEVSGDVKINL
EKMIARKQAVVDQTVGGINYLMEKNKITVFNGLGSFVDATHIAVAKADGTSETIEAKYTVIATGSKPSSLPFIKIDKERI
ITSTEALALKEVPKHLVIIGGGVIGIELGQVYLRLGAQVSVVEFMDRIIPGMDSSLSKELTKVLKKQGMKFYVSHKVKSV
ERNGDAVVVQAENAKGETITLEGDYSLVSVGRRPYTDGLNADKAGVKISDRGQVEVNDHLQTSVPNIYAIGDVVRGAMLA
HKAEEEGVMVAEILAGQKPHIDYNLIPGVVYTWPEVAAVGQTEEQLKAAGVKYKSGSFPFKALGRARASADLDGFVKILA
DEKTDEVLGVHMIGARTADLIAEAVTAMEFKASAEDISRMSHAHPTFAEAVKEAALAATENRALHV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=49.5744680851064, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=29.5652173913043, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=29.5010845986985, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI33519430, Length=430, Percent_Identity=27.6744186046512, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI33519428, Length=430, Percent_Identity=27.6744186046512, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI33519426, Length=430, Percent_Identity=27.6744186046512, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI148277065, Length=430, Percent_Identity=27.6744186046512, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI148277071, Length=430, Percent_Identity=27.6744186046512, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI291045266, Length=428, Percent_Identity=27.1028037383178, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI291045268, Length=422, Percent_Identity=25.5924170616114, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=43.9024390243902, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=31.6702819956616, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1789915, Length=441, Percent_Identity=30.3854875283447, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=27.6824034334764, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=51.3978494623656, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=27.2727272727273, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=359, Percent_Identity=28.6908077994429, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=359, Percent_Identity=28.6908077994429, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=24.9488752556237, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=188, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=51.1578947368421, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=31.6561844863732, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=27.645788336933, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=53.9614561027837, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24640549, Length=481, Percent_Identity=27.4428274428274, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24640553, Length=481, Percent_Identity=27.4428274428274, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=28.3643892339545, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI17737741, Length=495, Percent_Identity=26.4646464646465, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49817; Mature: 49686

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFDVVIIGSGPGGYVSAIRCAQLGFKTAIVEKYNSLGGTCLNVGCIPSKALLSSSHHY
CCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCH
AEIAHFADHGIEVSGDVKINLEKMIARKQAVVDQTVGGINYLMEKNKITVFNGLGSFVDA
HHHHHHHHCCEEECCCEEEEHHHHHHHHHHHHHHHCCCHHHHEECCCEEEEECCCCHHHH
THIAVAKADGTSETIEAKYTVIATGSKPSSLPFIKIDKERIITSTEALALKEVPKHLVII
HEEEEEECCCCCCEEEEEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEEEE
GGGVIGIELGQVYLRLGAQVSVVEFMDRIIPGMDSSLSKELTKVLKKQGMKFYVSHKVKS
ECCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHH
VERNGDAVVVQAENAKGETITLEGDYSLVSVGRRPYTDGLNADKAGVKISDRGQVEVNDH
HCCCCCEEEEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCC
LQTSVPNIYAIGDVVRGAMLAHKAEEEGVMVAEILAGQKPHIDYNLIPGVVYTWPEVAAV
HHCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCEEECCCHHHCC
GQTEEQLKAAGVKYKSGSFPFKALGRARASADLDGFVKILADEKTDEVLGVHMIGARTAD
CCCHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHHHHEEECCCCCCEEEEEEECCHHHH
LIAEAVTAMEFKASAEDISRMSHAHPTFAEAVKEAALAATENRALHV
HHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
SSFDVVIIGSGPGGYVSAIRCAQLGFKTAIVEKYNSLGGTCLNVGCIPSKALLSSSHHY
CCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCH
AEIAHFADHGIEVSGDVKINLEKMIARKQAVVDQTVGGINYLMEKNKITVFNGLGSFVDA
HHHHHHHHCCEEECCCEEEEHHHHHHHHHHHHHHHCCCHHHHEECCCEEEEECCCCHHHH
THIAVAKADGTSETIEAKYTVIATGSKPSSLPFIKIDKERIITSTEALALKEVPKHLVII
HEEEEEECCCCCCEEEEEEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEEEE
GGGVIGIELGQVYLRLGAQVSVVEFMDRIIPGMDSSLSKELTKVLKKQGMKFYVSHKVKS
ECCEEEEHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHH
VERNGDAVVVQAENAKGETITLEGDYSLVSVGRRPYTDGLNADKAGVKISDRGQVEVNDH
HCCCCCEEEEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCC
LQTSVPNIYAIGDVVRGAMLAHKAEEEGVMVAEILAGQKPHIDYNLIPGVVYTWPEVAAV
HHCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCEEECCCHHHCC
GQTEEQLKAAGVKYKSGSFPFKALGRARASADLDGFVKILADEKTDEVLGVHMIGARTAD
CCCHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHHHHEEECCCCCCEEEEEEECCHHHH
LIAEAVTAMEFKASAEDISRMSHAHPTFAEAVKEAALAATENRALHV
HHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]