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The map label for this gene is srfAD [H]

Identifier: 146299857

GI number: 146299857

Start: 2531169

End: 2531888

Strand: Direct

Name: srfAD [H]

Synonym: Fjoh_2099

Alternate gene names: 146299857

Gene position: 2531169-2531888 (Clockwise)

Preceding gene: 146299856

Following gene: 146299858

Centisome position: 41.52

GC content: 33.33

Gene sequence:

>720_bases
ATGAAAAACCAGTTATTTTTTTTGCATTTCGCAGGAGGCAGTTCATTTTCGTTTGATTTTCTTAAAAACAAACTTAATAA
TAGTATTATGTCTATTGCTCTCGAATTACCGGGAAGAGGTAAAAGAATGGATGAAAAACTTATCAAACTAAAAGAAGAAG
CAATAGATGATTACTGTCGTCAGATTATCAAATTACGAAACGGAAATCCTTTTGTAATATACGGACATAGTATGGGCGCC
ACATTAGGACTAAGCGTAGTGGCAAGGTTAGAAAAAATGGGACATTTTCCTGAACAATTAGTTGTTTCAGGAAATGCAGG
CCCGGGAGTACGAAAGAAAAAAAACTTTATGACGTATTTATTAGGCGATTCTGAATTTAAAGAAGAATTAAGAGAACTTG
GAGGCGTTCCGGAAGAAGTCCTGAAAAATAACGAATTATACGATTTTTTTGCCCCTATAATCCGGGCTGATTTTGAATGT
CTGGAAAAAGATCCCTTTTTAGAAAAAGGCATAGTAATTAATACTCCAATTTATGCCCTGATGGGAACCGAAGAAGAAAC
ATGCAGTGAAATCGAGAATTGGAAAATATTTACAGATTCGGATTTTAGATACAATGTTTTAAAAGGAAACCACTTTTTTA
TATATCCCCATTCAGATCAAATAGCAATGATATTAAACAAGTCTTTTTCGGCTAAAAAAAGGGATTTATTGACAATTTAA

Upstream 100 bases:

>100_bases
TTCTTTAAACTATATGTTTGGAAATGATAAGATTAAATCTCAAAAAAGAAATTTTGGAAATGAAGAAGAAAGAAAAAGAA
GTAATTAATCAGTATGTATC

Downstream 100 bases:

>100_bases
AAATTTTAGAATATAAGGTTTAACAATTAAATTTTAAAAGGATGAATAATATAGCAAAAGAAGTTTATTGTATATCAGAT
GGATATGTGTATATATTGGG

Product: thioesterase

Products: NA

Alternate protein names: Cold shock protein CSI16 [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKNQLFFLHFAGGSSFSFDFLKNKLNNSIMSIALELPGRGKRMDEKLIKLKEEAIDDYCRQIIKLRNGNPFVIYGHSMGA
TLGLSVVARLEKMGHFPEQLVVSGNAGPGVRKKKNFMTYLLGDSEFKEELRELGGVPEEVLKNNELYDFFAPIIRADFEC
LEKDPFLEKGIVINTPIYALMGTEEETCSEIENWKIFTDSDFRYNVLKGNHFFIYPHSDQIAMILNKSFSAKKRDLLTI

Sequences:

>Translated_239_residues
MKNQLFFLHFAGGSSFSFDFLKNKLNNSIMSIALELPGRGKRMDEKLIKLKEEAIDDYCRQIIKLRNGNPFVIYGHSMGA
TLGLSVVARLEKMGHFPEQLVVSGNAGPGVRKKKNFMTYLLGDSEFKEELRELGGVPEEVLKNNELYDFFAPIIRADFEC
LEKDPFLEKGIVINTPIYALMGTEEETCSEIENWKIFTDSDFRYNVLKGNHFFIYPHSDQIAMILNKSFSAKKRDLLTI
>Mature_239_residues
MKNQLFFLHFAGGSSFSFDFLKNKLNNSIMSIALELPGRGKRMDEKLIKLKEEAIDDYCRQIIKLRNGNPFVIYGHSMGA
TLGLSVVARLEKMGHFPEQLVVSGNAGPGVRKKKNFMTYLLGDSEFKEELRELGGVPEEVLKNNELYDFFAPIIRADFEC
LEKDPFLEKGIVINTPIYALMGTEEETCSEIENWKIFTDSDFRYNVLKGNHFFIYPHSDQIAMILNKSFSAKKRDLLTI

Specific function: Probable thioesterase involved in the biosynthesis of surfactin [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=220, Percent_Identity=25.9090909090909, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 27338; Mature: 27338

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQLFFLHFAGGSSFSFDFLKNKLNNSIMSIALELPGRGKRMDEKLIKLKEEAIDDYCR
CCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
QIIKLRNGNPFVIYGHSMGATLGLSVVARLEKMGHFPEQLVVSGNAGPGVRKKKNFMTYL
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCEEEEE
LGDSEFKEELRELGGVPEEVLKNNELYDFFAPIIRADFECLEKDPFLEKGIVINTPIYAL
ECCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCCEEEECCEEEE
MGTEEETCSEIENWKIFTDSDFRYNVLKGNHFFIYPHSDQIAMILNKSFSAKKRDLLTI
ECCCHHHHHHHHCCEEEECCCCEEEEEECCEEEEEECCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKNQLFFLHFAGGSSFSFDFLKNKLNNSIMSIALELPGRGKRMDEKLIKLKEEAIDDYCR
CCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
QIIKLRNGNPFVIYGHSMGATLGLSVVARLEKMGHFPEQLVVSGNAGPGVRKKKNFMTYL
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCEEEEE
LGDSEFKEELRELGGVPEEVLKNNELYDFFAPIIRADFECLEKDPFLEKGIVINTPIYAL
ECCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCCEEEECCEEEE
MGTEEETCSEIENWKIFTDSDFRYNVLKGNHFFIYPHSDQIAMILNKSFSAKKRDLLTI
ECCCHHHHHHHHCCEEEECCCCEEEEEECCEEEEEECCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8355609; 8969502; 9384377 [H]