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The map label for this gene is gsp [H]

Identifier: 146299844

GI number: 146299844

Start: 2474794

End: 2475426

Strand: Direct

Name: gsp [H]

Synonym: Fjoh_2086

Alternate gene names: 146299844

Gene position: 2474794-2475426 (Clockwise)

Preceding gene: 146299843

Following gene: 146299845

Centisome position: 40.59

GC content: 31.44

Gene sequence:

>633_bases
TTGATTTACATATATTACTCTTATATCGCTGAGGATAATTGTGAAAGCCTTATTAAAAATGAGCTGCCAAAATTTTCATC
AGATTTTCAGAATAGAATAAAAAGCTATCGCAGATGGCAGGATGCCCAATTATCCCTCTTGGGCAGGATAATTTTATTTA
AGGCAGTTGACCATATCTATAATGGTAAATTTCAAAGTAGGGAAATAGAGCAGACGCAGTACAATAAGCCTTACTTTAAA
AACGGTTCTATTCAATTTAATATTTCTCATTCTGGAGAGATCGTAGTTTGTGCTTTATGTGAAAAATCACAAGTAGGAAT
TGATATAGAATTTATAACAGATGTTTTAATTGATGACTGTAAATGGCAGATGACTGAAAATGAATGGCTGACCCTTACTT
CATCAGATAAAATAAAAGAAGCATTTTTTGGATACTGGACACAAAAAGAAGCGGTTATAAAAGCAAATGGACTGGGATTA
TCAATACCATTAAAAACATTCGAGGTTTTAGAAGAGACGGCAATTATTAAAGGCCAAAAATTTTTCTTAAAAGAAATTGA
AATTGATGAAAAGTATAAATGCTATATCGCTTTGGATAGAGAAATAGATCAGATCTGTGTCAGAAAAATATAG

Upstream 100 bases:

>100_bases
TAATGTGGTATTATGTTTTAACAAAACTTAATTGATATCACTATAGAGATTAGAATAACGAGAACTTAATTCCTGCCTTT
AATTTAAAACCGATACAGTT

Downstream 100 bases:

>100_bases
GATAAGTGTTTTTTAAGTATTAACCATTAAAAAAAAATTATGAATAAGAAAATTTTAGTTATAGGTGCATGCGGTCAGAT
TGGCACAGAACTTACCTTGA

Product: 4'-phosphopantetheinyl transferase

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: Gramicidin synthase-activating enzyme [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIYIYYSYIAEDNCESLIKNELPKFSSDFQNRIKSYRRWQDAQLSLLGRIILFKAVDHIYNGKFQSREIEQTQYNKPYFK
NGSIQFNISHSGEIVVCALCEKSQVGIDIEFITDVLIDDCKWQMTENEWLTLTSSDKIKEAFFGYWTQKEAVIKANGLGL
SIPLKTFEVLEETAIIKGQKFFLKEIEIDEKYKCYIALDREIDQICVRKI

Sequences:

>Translated_210_residues
MIYIYYSYIAEDNCESLIKNELPKFSSDFQNRIKSYRRWQDAQLSLLGRIILFKAVDHIYNGKFQSREIEQTQYNKPYFK
NGSIQFNISHSGEIVVCALCEKSQVGIDIEFITDVLIDDCKWQMTENEWLTLTSSDKIKEAFFGYWTQKEAVIKANGLGL
SIPLKTFEVLEETAIIKGQKFFLKEIEIDEKYKCYIALDREIDQICVRKI
>Mature_210_residues
MIYIYYSYIAEDNCESLIKNELPKFSSDFQNRIKSYRRWQDAQLSLLGRIILFKAVDHIYNGKFQSREIEQTQYNKPYFK
NGSIQFNISHSGEIVVCALCEKSQVGIDIEFITDVLIDDCKWQMTENEWLTLTSSDKIKEAFFGYWTQKEAVIKANGLGL
SIPLKTFEVLEETAIIKGQKFFLKEIEIDEKYKCYIALDREIDQICVRKI

Specific function: Activates the five peptidyl carrier protein (PCP) domains of gramicidin synthase GrsAB, by transferring the 4'- phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for gramicidin S production [H]

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR004568 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 24667; Mature: 24667

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYIYYSYIAEDNCESLIKNELPKFSSDFQNRIKSYRRWQDAQLSLLGRIILFKAVDHIY
CEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NGKFQSREIEQTQYNKPYFKNGSIQFNISHSGEIVVCALCEKSQVGIDIEFITDVLIDDC
CCCCCCCCCHHHHCCCCEEECCEEEEEECCCCCEEEEEECCCCCCCEEHHHHHHHHHHCC
KWQMTENEWLTLTSSDKIKEAFFGYWTQKEAVIKANGLGLSIPLKTFEVLEETAIIKGQK
CEEECCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHHCCCH
FFLKEIEIDEKYKCYIALDREIDQICVRKI
HEEEECCCCCCEEEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MIYIYYSYIAEDNCESLIKNELPKFSSDFQNRIKSYRRWQDAQLSLLGRIILFKAVDHIY
CEEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NGKFQSREIEQTQYNKPYFKNGSIQFNISHSGEIVVCALCEKSQVGIDIEFITDVLIDDC
CCCCCCCCCHHHHCCCCEEECCEEEEEECCCCCEEEEEECCCCCCCEEHHHHHHHHHHCC
KWQMTENEWLTLTSSDKIKEAFFGYWTQKEAVIKANGLGLSIPLKTFEVLEETAIIKGQK
CEEECCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHHCCCH
FFLKEIEIDEKYKCYIALDREIDQICVRKI
HEEEECCCCCCEEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7512553; 2477357; 8939709 [H]