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The map label for this gene is lacZ [H]

Identifier: 146299783

GI number: 146299783

Start: 2373411

End: 2375222

Strand: Direct

Name: lacZ [H]

Synonym: Fjoh_2024

Alternate gene names: 146299783

Gene position: 2373411-2375222 (Clockwise)

Preceding gene: 146299782

Following gene: 146299784

Centisome position: 38.93

GC content: 37.09

Gene sequence:

>1812_bases
ATGAAAAGAAAAAGTATCCTTTTTTTAGCCTTGTTATCCATGTTTGCAGTAAATGCGCAATGGAAACCTCAGGGAGATAA
AATCAAAACAAAATGGGCAGAACAGGTTGATGCAAAGAAACCGCTGCCAGAATATCCCCGTCCGATTATGGAGCGCAGCC
AATGGAAAAATCTAAATGGTTTATGGAATTATGCCATTCAGGAATTTGGCAAAACTGCGCCTTCAAAATATGACGGACAG
ATTCTGGTTCCGTTTGCGGCAGAATCAAGTCTGTCTGGTGTGATGAAAGAAGTCGGTGCAAAAAATGAACTTTGGTACGA
AACTAATTTTTCGATTGAATCAGGCTGGAACGGCAAAAATATCCTTTTACATTTTGGAGCTGTTGACTGGAAAACAGAAG
TTTTTATAAACGACATTAAAGTAGGTTCGCATACTGGAGGTTATACACCATTTTCATTTGATATAACGCCATTTATTCAA
AAAGGGAAAAATCAAAAACTAGTCGTTAAAGTTTGGGATCCATCAAACGACGGACCTCAGCCAAGAGGAAAACAAGTTAA
AAATCCAGAAGGAATTTGGTATACGCCTGTTACCGGAATCTGGCAGACCGTTTGGATTGAGCCAGTAAACGCTAAAAATA
TTACAGAACTAAAAACAACACCCAATATTGACCAAAATACAATCAGCATTAAGGCTGATATAAACGGAGCAGAAAAAGGA
GATTTAGTTGAAATTTCTGTTTTTGACGGTGGTAAAGAAATTGCCAAAGAAAAAGCTGTTGTTGGCGAAAGCAACGATAT
TGTTTTGAATAACCCAAAATTATGGTCGCCTGAAAGTCCGTTTTTATATCAGACTAAAGTTCGTTTAATCAGCAAAAATA
AAGTGGTTGATGAGGTGAAAAGTTATTTTGCGATGCGAAAAATTTCATCTAAACGAGATGAAAACGGAATCATGAGAATG
CAGTTAAACAATAAAGATTATTTTCAATTCGGACCTTTAGACCAAGGCTGGTGGCCAGACGGATTATACACTGCACCAAC
TGATGAAGCTTTAAAATACGATATTATTAAAACCAAAGAATTAGGCTTTAATATGATTCGTAAACACGTAAAAGTTGAGC
CGGAACGCTGGTACACACATTGCGATCAATTAGGAATTTTGGTTTGGCAGGATATGCCAAGCGGAGACGATCAGCCGATT
TGGCAAAACACAAAATATTTTACCGGAACAGAATTACAGCGTTCGGCAAAATCAGAAGAAATCTACAGAAAAGAATGGAA
AGAAATTATGGATCATTTGTATTCCTATCCAAGTATTGTTGTTTGGGTTCCGTTTAACGAAGCCTGGGGACAATTCAAAA
CCGTAGAAATTACAGAATGGACTAAAAACCACGATCCAAGCCGATTAACAAATTCGTCAAGCGGCGGGAATCATTTTCAA
ACCGGAGACATTTTAGATTTACACAATTATCCCGGACCAGAAATGTATTTGTATGATGCCAGAAGAGTTACTGTTTTAGG
AGAATACGGCGGGATTGGTCTTCCGCTGGAAGGGCATTTATGGAGAGCAAATGACAATTGGGGTTACGTTAAATTTAAAA
ATGAAGCTGAAGTAACGGCAGAATATATCAAGTATGCTAAAATCTTAAAGAATTATGTAAAAACAGGATTTTCAGCAGCA
GTTTACACGCAGACAACCGATGTTGAAGGAGAAGTAAACGGTTTCATGACCTACGACAGAAAAATAGATAAAATGGATTT
CAAAGCTGTAAATAACATAAATTCTGAAGTTATAAATGCTTTGAATCATTAA

Upstream 100 bases:

>100_bases
AAGTTACCAGACTTATGTGGATATAAGTCTGGTAATTCATTTTGATTTAGAAGAATCACGGCATTTTTTGTTATCAAAAA
GTGCCTAAAATATATAAAGC

Downstream 100 bases:

>100_bases
ATAGTGATTTGTTTTTAAACACATAGAGACATAGATATTTTTATCTTAAAAAAGTGGAAGTAAAAGAAACTAGTTTCTAC
ATATAGTTGTACAGAGATAC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MKRKSILFLALLSMFAVNAQWKPQGDKIKTKWAEQVDAKKPLPEYPRPIMERSQWKNLNGLWNYAIQEFGKTAPSKYDGQ
ILVPFAAESSLSGVMKEVGAKNELWYETNFSIESGWNGKNILLHFGAVDWKTEVFINDIKVGSHTGGYTPFSFDITPFIQ
KGKNQKLVVKVWDPSNDGPQPRGKQVKNPEGIWYTPVTGIWQTVWIEPVNAKNITELKTTPNIDQNTISIKADINGAEKG
DLVEISVFDGGKEIAKEKAVVGESNDIVLNNPKLWSPESPFLYQTKVRLISKNKVVDEVKSYFAMRKISSKRDENGIMRM
QLNNKDYFQFGPLDQGWWPDGLYTAPTDEALKYDIIKTKELGFNMIRKHVKVEPERWYTHCDQLGILVWQDMPSGDDQPI
WQNTKYFTGTELQRSAKSEEIYRKEWKEIMDHLYSYPSIVVWVPFNEAWGQFKTVEITEWTKNHDPSRLTNSSSGGNHFQ
TGDILDLHNYPGPEMYLYDARRVTVLGEYGGIGLPLEGHLWRANDNWGYVKFKNEAEVTAEYIKYAKILKNYVKTGFSAA
VYTQTTDVEGEVNGFMTYDRKIDKMDFKAVNNINSEVINALNH

Sequences:

>Translated_603_residues
MKRKSILFLALLSMFAVNAQWKPQGDKIKTKWAEQVDAKKPLPEYPRPIMERSQWKNLNGLWNYAIQEFGKTAPSKYDGQ
ILVPFAAESSLSGVMKEVGAKNELWYETNFSIESGWNGKNILLHFGAVDWKTEVFINDIKVGSHTGGYTPFSFDITPFIQ
KGKNQKLVVKVWDPSNDGPQPRGKQVKNPEGIWYTPVTGIWQTVWIEPVNAKNITELKTTPNIDQNTISIKADINGAEKG
DLVEISVFDGGKEIAKEKAVVGESNDIVLNNPKLWSPESPFLYQTKVRLISKNKVVDEVKSYFAMRKISSKRDENGIMRM
QLNNKDYFQFGPLDQGWWPDGLYTAPTDEALKYDIIKTKELGFNMIRKHVKVEPERWYTHCDQLGILVWQDMPSGDDQPI
WQNTKYFTGTELQRSAKSEEIYRKEWKEIMDHLYSYPSIVVWVPFNEAWGQFKTVEITEWTKNHDPSRLTNSSSGGNHFQ
TGDILDLHNYPGPEMYLYDARRVTVLGEYGGIGLPLEGHLWRANDNWGYVKFKNEAEVTAEYIKYAKILKNYVKTGFSAA
VYTQTTDVEGEVNGFMTYDRKIDKMDFKAVNNINSEVINALNH
>Mature_603_residues
MKRKSILFLALLSMFAVNAQWKPQGDKIKTKWAEQVDAKKPLPEYPRPIMERSQWKNLNGLWNYAIQEFGKTAPSKYDGQ
ILVPFAAESSLSGVMKEVGAKNELWYETNFSIESGWNGKNILLHFGAVDWKTEVFINDIKVGSHTGGYTPFSFDITPFIQ
KGKNQKLVVKVWDPSNDGPQPRGKQVKNPEGIWYTPVTGIWQTVWIEPVNAKNITELKTTPNIDQNTISIKADINGAEKG
DLVEISVFDGGKEIAKEKAVVGESNDIVLNNPKLWSPESPFLYQTKVRLISKNKVVDEVKSYFAMRKISSKRDENGIMRM
QLNNKDYFQFGPLDQGWWPDGLYTAPTDEALKYDIIKTKELGFNMIRKHVKVEPERWYTHCDQLGILVWQDMPSGDDQPI
WQNTKYFTGTELQRSAKSEEIYRKEWKEIMDHLYSYPSIVVWVPFNEAWGQFKTVEITEWTKNHDPSRLTNSSSGGNHFQ
TGDILDLHNYPGPEMYLYDARRVTVLGEYGGIGLPLEGHLWRANDNWGYVKFKNEAEVTAEYIKYAKILKNYVKTGFSAA
VYTQTTDVEGEVNGFMTYDRKIDKMDFKAVNNINSEVINALNH

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787903, Length=253, Percent_Identity=24.1106719367589, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI48994920, Length=410, Percent_Identity=21.9512195121951, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1786539, Length=415, Percent_Identity=22.4096385542169, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17510825, Length=420, Percent_Identity=23.8095238095238, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 69188; Mature: 69188

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKSILFLALLSMFAVNAQWKPQGDKIKTKWAEQVDAKKPLPEYPRPIMERSQWKNLNG
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCH
LWNYAIQEFGKTAPSKYDGQILVPFAAESSLSGVMKEVGAKNELWYETNFSIESGWNGKN
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCE
ILLHFGAVDWKTEVFINDIKVGSHTGGYTPFSFDITPFIQKGKNQKLVVKVWDPSNDGPQ
EEEEECCCCCEEEEEEEEEEECCCCCCCCCEEECCCHHHHCCCCCEEEEEEECCCCCCCC
PRGKQVKNPEGIWYTPVTGIWQTVWIEPVNAKNITELKTTPNIDQNTISIKADINGAEKG
CCCCCCCCCCCEEECCHHHCEEEEEEEECCCCCCEEECCCCCCCCCEEEEEEECCCCCCC
DLVEISVFDGGKEIAKEKAVVGESNDIVLNNPKLWSPESPFLYQTKVRLISKNKVVDEVK
CEEEEEEECCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEEEHHHHHHHHHHH
SYFAMRKISSKRDENGIMRMQLNNKDYFQFGPLDQGWWPDGLYTAPTDEALKYDIIKTKE
HHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCCEEEEEEECHH
LGFNMIRKHVKVEPERWYTHCDQLGILVWQDMPSGDDQPIWQNTKYFTGTELQRSAKSEE
HHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEECCCHHHHHHHHHH
IYRKEWKEIMDHLYSYPSIVVWVPFNEAWGQFKTVEITEWTKNHDPSRLTNSSSGGNHFQ
HHHHHHHHHHHHHHCCCCEEEEEECHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
TGDILDLHNYPGPEMYLYDARRVTVLGEYGGIGLPLEGHLWRANDNWGYVKFKNEAEVTA
CCCEEEECCCCCCCEEEEECEEEEEEECCCCCCCCCCCCEEEECCCEEEEEECCCHHHHH
EYIKYAKILKNYVKTGFSAAVYTQTTDVEGEVNGFMTYDRKIDKMDFKAVNNINSEVINA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHCCCHHHHHH
LNH
HCC
>Mature Secondary Structure
MKRKSILFLALLSMFAVNAQWKPQGDKIKTKWAEQVDAKKPLPEYPRPIMERSQWKNLNG
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCH
LWNYAIQEFGKTAPSKYDGQILVPFAAESSLSGVMKEVGAKNELWYETNFSIESGWNGKN
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCE
ILLHFGAVDWKTEVFINDIKVGSHTGGYTPFSFDITPFIQKGKNQKLVVKVWDPSNDGPQ
EEEEECCCCCEEEEEEEEEEECCCCCCCCCEEECCCHHHHCCCCCEEEEEEECCCCCCCC
PRGKQVKNPEGIWYTPVTGIWQTVWIEPVNAKNITELKTTPNIDQNTISIKADINGAEKG
CCCCCCCCCCCEEECCHHHCEEEEEEEECCCCCCEEECCCCCCCCCEEEEEEECCCCCCC
DLVEISVFDGGKEIAKEKAVVGESNDIVLNNPKLWSPESPFLYQTKVRLISKNKVVDEVK
CEEEEEEECCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEEEHHHHHHHHHHH
SYFAMRKISSKRDENGIMRMQLNNKDYFQFGPLDQGWWPDGLYTAPTDEALKYDIIKTKE
HHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCCCCEEEEEEECHH
LGFNMIRKHVKVEPERWYTHCDQLGILVWQDMPSGDDQPIWQNTKYFTGTELQRSAKSEE
HHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEECCCHHHHHHHHHH
IYRKEWKEIMDHLYSYPSIVVWVPFNEAWGQFKTVEITEWTKNHDPSRLTNSSSGGNHFQ
HHHHHHHHHHHHHHCCCCEEEEEECHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
TGDILDLHNYPGPEMYLYDARRVTVLGEYGGIGLPLEGHLWRANDNWGYVKFKNEAEVTA
CCCEEEECCCCCCCEEEEECEEEEEEECCCCCCCCCCCCEEEECCCEEEEEECCCHHHHH
EYIKYAKILKNYVKTGFSAAVYTQTTDVEGEVNGFMTYDRKIDKMDFKAVNNINSEVINA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHCCCHHHHHH
LNH
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA