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The map label for this gene is cbs [H]

Identifier: 146299687

GI number: 146299687

Start: 2229859

End: 2231220

Strand: Direct

Name: cbs [H]

Synonym: Fjoh_1927

Alternate gene names: 146299687

Gene position: 2229859-2231220 (Clockwise)

Preceding gene: 146299686

Following gene: 146299688

Centisome position: 36.57

GC content: 34.36

Gene sequence:

>1362_bases
ATGGACTTTTCAAATAATATTTTAGAAACAATTGGTAACACACCATTGGTAAAGCTCAACAAAATTGTTGCTGAAATTGA
TGCGTTAGTATTGGCAAAAGTCGAAACATTTAATCCGGGAAACTCTGTAAAAGACAGAATGGCCGTAAAAATGATTGAAG
ATGCTGAGGCTGACGGAAGACTTAAACCAGGAGGAACTATAATTGAAGGTACTTCTGGAAATACTGGAATGGGACTGGCA
CTTGTAGCAATCGTAAAAGGGTACAAATTAATCTGTGTAATCTCAGATAAACAGTCAAAAGAAAAAATGGATATACTTCG
TGCTGTTGGAGCAAAAGTTGTAGTTTGCCCTACAGATGTTGAGCCTACAGATCCACGTTCGTATTATTCAGTTTCAAAAC
GTTTGGCCGAAGAAACGCCAAATTCATGGTATGTAAACCAGTATGATAATATGTCAAATTCGTTGGCACATTATGAGCAG
ACCGGCCCGGAAATCTGGAAACAGACAGACGGAAAAATTACACATTTTGTTGTAGGGGTTGGAACTGGAGGAACAATTTC
TGGAGTTGGAAAATACTTAAAAGAAAAAAATCCAAATATTAAAATCTGGGGAATAGATACTTATGGTTCTGTTTTTAAGA
AATACCATGAAACGGGAATTTTTGACGAAAACGAAATTTACTCATACATAACAGAAGGAATTGGAGAAGATATTTTACCT
AAAAATGTTGATTTCTCCTTAATTGATGGTTTTACAAAAGTAACCGATAAAGATGCCGCAGTTTATACAAGAAAAATTGC
TCTTGAAGAAGCTATTTTTGTTGGTAATTCTGCAGGAGCCTGCATAAAAGGATTACTTCAGTTAAAAGAACACTTTAAAC
CAGATGATGTTGTTGTTGTTTTATTTCATGATTCAGGAAGCCGTTATGTAGGTAAAATGTTTAATGATGACTGGATGCGT
GAACGCGGATTCTTAGAAGAAAATGTCACGAAAGCAGAAGATGTTATTAAAGATCACATCGACAAAGAATTAATTGTTGT
TCGTACAGAAGAATTAGTTTCTCATGCCATTGAGCGTATGCGTAAATATAAAATTTCGCAGATTCCGGTTGTTGATATAA
ACGGTTTTGTTGGTTCTGTAGATGAAACAGATTTGTTTAGAAGTTATGTTGCTGATAAAAACGTAGCCGAAAAACCAATT
AAAGAAGTAATGGGAAAACCTTTTCCAATTGTAAAATTAGGAACACCAATCGAAGAAGTATCAAAACTATTTACCAAAGA
AAATGATGCAGTTCTTGTTGATTTAGGAAACGGAAACCATCACATTATTACAAAATATGATATTATTGGCTCTATAAAAT
AG

Upstream 100 bases:

>100_bases
TAATCCGTATCGTGATTTTGTTATCAGATTTGGTTTAGTTTGGAATTTCTTCCAATAAAAATCATACAATTATTTAAATA
CCACATTAAACTTAAATAAA

Downstream 100 bases:

>100_bases
GCTTATAAATAAAACTTTAATCCACAAATCAGATTGAATCGGGTTTGTGGATTTTTTTATTCCTTAAAATAAATTTGTAA
GAATAATATTAAATTTATTA

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MDFSNNILETIGNTPLVKLNKIVAEIDALVLAKVETFNPGNSVKDRMAVKMIEDAEADGRLKPGGTIIEGTSGNTGMGLA
LVAIVKGYKLICVISDKQSKEKMDILRAVGAKVVVCPTDVEPTDPRSYYSVSKRLAEETPNSWYVNQYDNMSNSLAHYEQ
TGPEIWKQTDGKITHFVVGVGTGGTISGVGKYLKEKNPNIKIWGIDTYGSVFKKYHETGIFDENEIYSYITEGIGEDILP
KNVDFSLIDGFTKVTDKDAAVYTRKIALEEAIFVGNSAGACIKGLLQLKEHFKPDDVVVVLFHDSGSRYVGKMFNDDWMR
ERGFLEENVTKAEDVIKDHIDKELIVVRTEELVSHAIERMRKYKISQIPVVDINGFVGSVDETDLFRSYVADKNVAEKPI
KEVMGKPFPIVKLGTPIEEVSKLFTKENDAVLVDLGNGNHHIITKYDIIGSIK

Sequences:

>Translated_453_residues
MDFSNNILETIGNTPLVKLNKIVAEIDALVLAKVETFNPGNSVKDRMAVKMIEDAEADGRLKPGGTIIEGTSGNTGMGLA
LVAIVKGYKLICVISDKQSKEKMDILRAVGAKVVVCPTDVEPTDPRSYYSVSKRLAEETPNSWYVNQYDNMSNSLAHYEQ
TGPEIWKQTDGKITHFVVGVGTGGTISGVGKYLKEKNPNIKIWGIDTYGSVFKKYHETGIFDENEIYSYITEGIGEDILP
KNVDFSLIDGFTKVTDKDAAVYTRKIALEEAIFVGNSAGACIKGLLQLKEHFKPDDVVVVLFHDSGSRYVGKMFNDDWMR
ERGFLEENVTKAEDVIKDHIDKELIVVRTEELVSHAIERMRKYKISQIPVVDINGFVGSVDETDLFRSYVADKNVAEKPI
KEVMGKPFPIVKLGTPIEEVSKLFTKENDAVLVDLGNGNHHIITKYDIIGSIK
>Mature_453_residues
MDFSNNILETIGNTPLVKLNKIVAEIDALVLAKVETFNPGNSVKDRMAVKMIEDAEADGRLKPGGTIIEGTSGNTGMGLA
LVAIVKGYKLICVISDKQSKEKMDILRAVGAKVVVCPTDVEPTDPRSYYSVSKRLAEETPNSWYVNQYDNMSNSLAHYEQ
TGPEIWKQTDGKITHFVVGVGTGGTISGVGKYLKEKNPNIKIWGIDTYGSVFKKYHETGIFDENEIYSYITEGIGEDILP
KNVDFSLIDGFTKVTDKDAAVYTRKIALEEAIFVGNSAGACIKGLLQLKEHFKPDDVVVVLFHDSGSRYVGKMFNDDWMR
ERGFLEENVTKAEDVIKDHIDKELIVVRTEELVSHAIERMRKYKISQIPVVDINGFVGSVDETDLFRSYVADKNVAEKPI
KEVMGKPFPIVKLGTPIEEVSKLFTKENDAVLVDLGNGNHHIITKYDIIGSIK

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=437, Percent_Identity=39.1304347826087, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI295821200, Length=437, Percent_Identity=39.1304347826087, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI4557415, Length=437, Percent_Identity=39.1304347826087, Blast_Score=297, Evalue=1e-80,
Organism=Escherichia coli, GI2367138, Length=306, Percent_Identity=37.5816993464052, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788754, Length=322, Percent_Identity=35.7142857142857, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17562970, Length=321, Percent_Identity=38.006230529595, Blast_Score=210, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32566674, Length=310, Percent_Identity=38.7096774193548, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17535051, Length=310, Percent_Identity=37.741935483871, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17534315, Length=330, Percent_Identity=40.3030303030303, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI25147552, Length=330, Percent_Identity=38.7878787878788, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17561720, Length=318, Percent_Identity=36.1635220125786, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI115535073, Length=340, Percent_Identity=35.2941176470588, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32566672, Length=230, Percent_Identity=33.9130434782609, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71996324, Length=319, Percent_Identity=23.1974921630094, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539640, Length=223, Percent_Identity=28.6995515695067, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=502, Percent_Identity=33.6653386454183, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321449, Length=330, Percent_Identity=32.7272727272727, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643623, Length=414, Percent_Identity=37.9227053140097, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI20129101, Length=414, Percent_Identity=37.9227053140097, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 50280; Mature: 50280

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSNNILETIGNTPLVKLNKIVAEIDALVLAKVETFNPGNSVKDRMAVKMIEDAEADGR
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCE
LKPGGTIIEGTSGNTGMGLALVAIVKGYKLICVISDKQSKEKMDILRAVGAKVVVCPTDV
ECCCCEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCC
EPTDPRSYYSVSKRLAEETPNSWYVNQYDNMSNSLAHYEQTGPEIWKQTDGKITHFVVGV
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCHHHHHCCCCCEEEEEEEE
GTGGTISGVGKYLKEKNPNIKIWGIDTYGSVFKKYHETGIFDENEIYSYITEGIGEDILP
CCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
KNVDFSLIDGFTKVTDKDAAVYTRKIALEEAIFVGNSAGACIKGLLQLKEHFKPDDVVVV
CCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCEEEE
LFHDSGSRYVGKMFNDDWMRERGFLEENVTKAEDVIKDHIDKELIVVRTEELVSHAIERM
EEECCCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHH
RKYKISQIPVVDINGFVGSVDETDLFRSYVADKNVAEKPIKEVMGKPFPIVKLGTPIEEV
HHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCHHHH
SKLFTKENDAVLVDLGNGNHHIITKYDIIGSIK
HHHHCCCCCEEEEEECCCCEEEEEEEEEEECCC
>Mature Secondary Structure
MDFSNNILETIGNTPLVKLNKIVAEIDALVLAKVETFNPGNSVKDRMAVKMIEDAEADGR
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCE
LKPGGTIIEGTSGNTGMGLALVAIVKGYKLICVISDKQSKEKMDILRAVGAKVVVCPTDV
ECCCCEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCC
EPTDPRSYYSVSKRLAEETPNSWYVNQYDNMSNSLAHYEQTGPEIWKQTDGKITHFVVGV
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCHHHHHCCCCCEEEEEEEE
GTGGTISGVGKYLKEKNPNIKIWGIDTYGSVFKKYHETGIFDENEIYSYITEGIGEDILP
CCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
KNVDFSLIDGFTKVTDKDAAVYTRKIALEEAIFVGNSAGACIKGLLQLKEHFKPDDVVVV
CCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCEEEE
LFHDSGSRYVGKMFNDDWMRERGFLEENVTKAEDVIKDHIDKELIVVRTEELVSHAIERM
EEECCCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHH
RKYKISQIPVVDINGFVGSVDETDLFRSYVADKNVAEKPIKEVMGKPFPIVKLGTPIEEV
HHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCHHHH
SKLFTKENDAVLVDLGNGNHHIITKYDIIGSIK
HHHHCCCCCEEEEEECCCCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]