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The map label for this gene is lipB

Identifier: 146299682

GI number: 146299682

Start: 2221259

End: 2221957

Strand: Direct

Name: lipB

Synonym: Fjoh_1922

Alternate gene names: 146299682

Gene position: 2221259-2221957 (Clockwise)

Preceding gene: 146299681

Following gene: 146299684

Centisome position: 36.43

GC content: 36.91

Gene sequence:

>699_bases
ATGAATAAAAAAATTCAACTTCAGGATCTGGGAAACAGAGACTATAAATCGACTTGGGAATATCAGGAAGAACTTTTTCA
GGATATTGTCGATTTAAAAATCAAGAACAGAAGAGAAGAACTGGACTTGCCAACATCCAATTATTTATTGTTTGTTGAGC
ATCCGCATGTTTATACTTTAGGTAAAAGCGGCGATTTAGAAAACTTATTATTAAACGAAAAACAGCTAGAAGCAAAAGGA
GCAGCTTTTTATAAAATTAATCGCGGCGGTGATATCACATATCACGGACCGGGACAAATTGTTGGATATCCAATTCTGGA
CTTAGAAAATTTCTTTACGGATATTCATAAATACCTGCGTTTACTTGAAGAATCTATGATTCTGACTCTGGCAGAATACG
GATTAGAATCTGGCAGAAGCGAGGGCGAAACAGGAGTCTGGCTGGGAGTTGGAACTCCGTTTGCACGTAAAATATGCGCA
ATGGGCGTTCGTGCTTCGCGCTGGGTAACAATGCACGGATTTGCTTTAAATGTAAATGTAGATTTAGGTTATTTTGATAA
TATTATTCCGTGCGGCATTCGTGGAAAAGGTGTTACGTCTTTAAACGTTGAACTTGGCGTAGAAAAAGTAGATGAAGACG
AAGTAAAGTCTAAAATCATAAAACATTTAACCCATTTGTTTGAAGCTGAAATAGTTTAA

Upstream 100 bases:

>100_bases
GCGACGACCGAAAATCAATTACAATTCAATACCCTTTCACGGATTATATGGAGAATCCTGACAAACTTAACTTTGATGTT
GAGTTTGAAAAATAATTAAA

Downstream 100 bases:

>100_bases
AATAAAAAAGACTTATCATTTTGATAAGTCTTTTTTTATTCGAAAAAATCAAGCTCTAATTGATCCGGCAGAAGTCTAAA
ACTCATTCGGTGATATTTTG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNKKIQLQDLGNRDYKSTWEYQEELFQDIVDLKIKNRREELDLPTSNYLLFVEHPHVYTLGKSGDLENLLLNEKQLEAKG
AAFYKINRGGDITYHGPGQIVGYPILDLENFFTDIHKYLRLLEESMILTLAEYGLESGRSEGETGVWLGVGTPFARKICA
MGVRASRWVTMHGFALNVNVDLGYFDNIIPCGIRGKGVTSLNVELGVEKVDEDEVKSKIIKHLTHLFEAEIV

Sequences:

>Translated_232_residues
MNKKIQLQDLGNRDYKSTWEYQEELFQDIVDLKIKNRREELDLPTSNYLLFVEHPHVYTLGKSGDLENLLLNEKQLEAKG
AAFYKINRGGDITYHGPGQIVGYPILDLENFFTDIHKYLRLLEESMILTLAEYGLESGRSEGETGVWLGVGTPFARKICA
MGVRASRWVTMHGFALNVNVDLGYFDNIIPCGIRGKGVTSLNVELGVEKVDEDEVKSKIIKHLTHLFEAEIV
>Mature_232_residues
MNKKIQLQDLGNRDYKSTWEYQEELFQDIVDLKIKNRREELDLPTSNYLLFVEHPHVYTLGKSGDLENLLLNEKQLEAKG
AAFYKINRGGDITYHGPGQIVGYPILDLENFFTDIHKYLRLLEESMILTLAEYGLESGRSEGETGVWLGVGTPFARKICA
MGVRASRWVTMHGFALNVNVDLGYFDNIIPCGIRGKGVTSLNVELGVEKVDEDEVKSKIIKHLTHLFEAEIV

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=178, Percent_Identity=37.0786516853933, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI87081767, Length=192, Percent_Identity=34.375, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323268, Length=168, Percent_Identity=31.547619047619, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355483, Length=186, Percent_Identity=40.3225806451613, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_FLAJ1 (A5FIL6)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194273.1
- ProteinModelPortal:   A5FIL6
- SMR:   A5FIL6
- STRING:   A5FIL6
- GeneID:   5091658
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1922
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   TEDHERP
- ProtClustDB:   CLSK956213
- BioCyc:   FJOH376686:FJOH_1922-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 26364; Mature: 26364

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIQLQDLGNRDYKSTWEYQEELFQDIVDLKIKNRREELDLPTSNYLLFVEHPHVYTL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCEEEEE
GKSGDLENLLLNEKQLEAKGAAFYKINRGGDITYHGPGQIVGYPILDLENFFTDIHKYLR
CCCCCHHHHHHCHHHHHCCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHH
LLEESMILTLAEYGLESGRSEGETGVWLGVGTPFARKICAMGVRASRWVTMHGFALNVNV
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEEEEEEEEE
DLGYFDNIIPCGIRGKGVTSLNVELGVEKVDEDEVKSKIIKHLTHLFEAEIV
ECCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKKIQLQDLGNRDYKSTWEYQEELFQDIVDLKIKNRREELDLPTSNYLLFVEHPHVYTL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCEEEEE
GKSGDLENLLLNEKQLEAKGAAFYKINRGGDITYHGPGQIVGYPILDLENFFTDIHKYLR
CCCCCHHHHHHCHHHHHCCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHH
LLEESMILTLAEYGLESGRSEGETGVWLGVGTPFARKICAMGVRASRWVTMHGFALNVNV
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEEEEEEEEE
DLGYFDNIIPCGIRGKGVTSLNVELGVEKVDEDEVKSKIIKHLTHLFEAEIV
ECCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA