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The map label for this gene is ispB [H]

Identifier: 146299544

GI number: 146299544

Start: 2064322

End: 2065299

Strand: Direct

Name: ispB [H]

Synonym: Fjoh_1784

Alternate gene names: 146299544

Gene position: 2064322-2065299 (Clockwise)

Preceding gene: 146299542

Following gene: 146299547

Centisome position: 33.86

GC content: 37.12

Gene sequence:

>978_bases
ATGAATATTACTTCACAAATAAAACAGCCCATTATTGCCGAGATGGAACTTTTTGAAAAAAAGTTCCATGAATCGATGAC
CTCAAAGGTTGCGTTACTCAACAGGATTACCTATTATATTGTAAACAGAAAAGGAAAACAAATGCGTCCGATGTTTGTGT
TTCTTACTGCAAAAATGGTTTCTGGCGGTACTGTAAACGAAAGAACCTATCGCGGGGCATCGGTTATTGAATTAATTCAT
ACGGCAACTTTGGTTCACGACGACGTTGTAGACGATAGTAATCGCCGCCGCGGATTTTTCTCAATCAATGCGCTTTGGAA
AAACAAAATTGCTGTTTTAGTTGGAGATTATCTTCTTTCAAAAGGATTACTGCTTTCTATTGATAACGGCGATTTTGATT
TACTTCGAATCATTTCTGTAGCCGTTCGCGAAATGAGCGAAGGTGAATTGCTTCAAATAGAAAAAGCACGCAGACTTGAC
ATTACAGAAGATGTTTACTACGAAATCATCAGAAAGAAAACCGCAACACTTATTGCTGCGTGTTGTGCACTTGGTGCAAA
AGCTGTAATTGAAGATGATGTTCAGGTTGAGAATATGCGAATGTTTGGTGAACTTATAGGAATGGCGTTCCAGATCAAAG
ACGACTTATTTGATTACAGCGAAGAAGCCATTGGTAAACCAACCGGAATCGATATTAAAGAGCAAAAAATGACACTGCCT
TTAATTCACGTTTTAAATACCTGCACACCGCAGGAAAAAAAGTGGTTGATAAACTCCATTAAAAACCATAACAAAGACAA
AAAACGAGTAAAAGAAGTAATTGCCTTTGTAAAAAACAATAATGGTTTGGCTTACGCCGAAGAAAAAATGGTGCAGTTCC
AGCAGGAAGCACTTTCTCTTTTAGAAAATTACCCGGATTCTGAATTCAAAGCAGCGCTTACCTTAATGGTAAACTATGTT
ATTGAAAGAAAAAAATAA

Upstream 100 bases:

>100_bases
GTGTTTTTGGATTGTTTTTTAAATTAGTATTGAAATTGTTGATATACTAACATAAAATAGACAACGTTTTTTTATTTAAA
ATAGTATATTTGGAATCGAA

Downstream 100 bases:

>100_bases
TTTTTTTTAAATTTATATCTCGATTAATGGATTATCTCCCAATATGGCTCAAATTGTAAATCATTAAAAGGAATACTGTG
TATATTGGCTGTTTCTCCTT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNITSQIKQPIIAEMELFEKKFHESMTSKVALLNRITYYIVNRKGKQMRPMFVFLTAKMVSGGTVNERTYRGASVIELIH
TATLVHDDVVDDSNRRRGFFSINALWKNKIAVLVGDYLLSKGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLD
ITEDVYYEIIRKKTATLIAACCALGAKAVIEDDVQVENMRMFGELIGMAFQIKDDLFDYSEEAIGKPTGIDIKEQKMTLP
LIHVLNTCTPQEKKWLINSIKNHNKDKKRVKEVIAFVKNNNGLAYAEEKMVQFQQEALSLLENYPDSEFKAALTLMVNYV
IERKK

Sequences:

>Translated_325_residues
MNITSQIKQPIIAEMELFEKKFHESMTSKVALLNRITYYIVNRKGKQMRPMFVFLTAKMVSGGTVNERTYRGASVIELIH
TATLVHDDVVDDSNRRRGFFSINALWKNKIAVLVGDYLLSKGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLD
ITEDVYYEIIRKKTATLIAACCALGAKAVIEDDVQVENMRMFGELIGMAFQIKDDLFDYSEEAIGKPTGIDIKEQKMTLP
LIHVLNTCTPQEKKWLINSIKNHNKDKKRVKEVIAFVKNNNGLAYAEEKMVQFQQEALSLLENYPDSEFKAALTLMVNYV
IERKK
>Mature_325_residues
MNITSQIKQPIIAEMELFEKKFHESMTSKVALLNRITYYIVNRKGKQMRPMFVFLTAKMVSGGTVNERTYRGASVIELIH
TATLVHDDVVDDSNRRRGFFSINALWKNKIAVLVGDYLLSKGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLD
ITEDVYYEIIRKKTATLIAACCALGAKAVIEDDVQVENMRMFGELIGMAFQIKDDLFDYSEEAIGKPTGIDIKEQKMTLP
LIHVLNTCTPQEKKWLINSIKNHNKDKKRVKEVIAFVKNNNGLAYAEEKMVQFQQEALSLLENYPDSEFKAALTLMVNYV
IERKK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=301, Percent_Identity=30.5647840531561, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI1789578, Length=313, Percent_Identity=33.5463258785943, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1786623, Length=215, Percent_Identity=29.7674418604651, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17505681, Length=304, Percent_Identity=26.6447368421053, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=302, Percent_Identity=26.4900662251656, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24651612, Length=304, Percent_Identity=32.2368421052632, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37152; Mature: 37152

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITSQIKQPIIAEMELFEKKFHESMTSKVALLNRITYYIVNRKGKQMRPMFVFLTAKMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEHHHH
SGGTVNERTYRGASVIELIHTATLVHDDVVDDSNRRRGFFSINALWKNKIAVLVGDYLLS
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCHHHHHHHHHHC
KGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLDITEDVYYEIIRKKTATLIAA
CCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECHHHCCCCHHHHHHHHHHHHHHHHHHH
CCALGAKAVIEDDVQVENMRMFGELIGMAFQIKDDLFDYSEEAIGKPTGIDIKEQKMTLP
HHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHH
LIHVLNTCTPQEKKWLINSIKNHNKDKKRVKEVIAFVKNNNGLAYAEEKMVQFQQEALSL
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LENYPDSEFKAALTLMVNYVIERKK
HHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNITSQIKQPIIAEMELFEKKFHESMTSKVALLNRITYYIVNRKGKQMRPMFVFLTAKMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEHHHH
SGGTVNERTYRGASVIELIHTATLVHDDVVDDSNRRRGFFSINALWKNKIAVLVGDYLLS
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCHHHHHHHHHHC
KGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLDITEDVYYEIIRKKTATLIAA
CCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECHHHCCCCHHHHHHHHHHHHHHHHHHH
CCALGAKAVIEDDVQVENMRMFGELIGMAFQIKDDLFDYSEEAIGKPTGIDIKEQKMTLP
HHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHH
LIHVLNTCTPQEKKWLINSIKNHNKDKKRVKEVIAFVKNNNGLAYAEEKMVQFQQEALSL
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LENYPDSEFKAALTLMVNYVIERKK
HHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]