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The map label for this gene is rlmN

Identifier: 146299542

GI number: 146299542

Start: 2062092

End: 2063141

Strand: Direct

Name: rlmN

Synonym: Fjoh_1782

Alternate gene names: 146299542

Gene position: 2062092-2063141 (Clockwise)

Preceding gene: 146299535

Following gene: 146299544

Centisome position: 33.82

GC content: 37.9

Gene sequence:

>1050_bases
ATGCAAATTGAGAAAAAAGACATAAGAGCCTTATCAAAAGATCAGTTACGAGATTTTTTTGTTGCTAATAACGACAAAGC
ATTTCGAGGCAATCAGGTTTATGAATGGTTGTGGAGCAAAGGAGCACATAGCTTTGAGGATATGACGAATGTTGCCAAAA
CAACGCGTTCTATGCTCGAAGAAAATTTCGTTATCAACCATATTAAGGTAGATACCATGCAGCGCAGTAACGACGGAACA
GTAAAAAATGCTGTGCGTCTGCATGACGGGTTAGTAGTAGAGTCAGTTTTAATTCCAACTGAGACCAGAACAACAGCTTG
TGTTTCTAGTCAGGTTGGCTGTAGTTTAGACTGTAATTTCTGTGCTACTGCAAGATTAAAACGTATGCGAAATCTGGAAC
CGGGAGAAATTTACGACCAGATTATGGCCATCGATAAAGAAAGTCGTTTGTATCATAATCATCCCCTTTCTAATATTGTT
TTTATGGGAATGGGCGAGCCTTTAATGAACTATAATAACGTCATTAAAGCAATCGATATGGTTACATCGGAAGAAGGTTT
AGGAATGTCTCCAAAACGTATTACGCTTTCAACATCCGGAATTCCGAAAATGATTAAGAAAATGGCCGATGATGATGTGA
AATTCAGATTGGCGGTTTCTCTTCATTCTGCGATTGATGAAACCCGTGCGAAGATTATGCCTTTCAGCAAAAACTTTCCT
TTAAAAGATTTAAGAGAAGCATTGGAATATTGGTACAGAAAAACCAAAAGCAAAGTTTCGTACGAATATGTAGTTTGGAA
AGGAATTAACGACGATAAAGCTTCTGTTGATGCTTTAGTGAAATTCTGTAAATATGTGCCGTGTAAAGTCAATTTAATTG
AATACAACCCAATTGATGACGGAGAATTCCAGCAAGCCTCAGAAGAATCTATTCTGGCTTACATAAAAGCATTAGAAAAT
ATTGGTGTTGTGGTAAAAGTGCGTCGCAGCCGAGGAAAAGATATCGATGCAGCCTGCGGACAATTAGCAAATAAAGAGGC
TGAAGTATAA

Upstream 100 bases:

>100_bases
TTGCGGGTAAAACAAGGATATTTTCTCTCAGATTTTTTGGTTTTAGCCAAGAATGCATCTAAAAATCAAAAAAAATGACA
AAAGTGATTAACTTTGCAGC

Downstream 100 bases:

>100_bases
TGCCTGCGGGATAAATATTCAGCTGGGAATATTTCGTTGGTAAAAAAATAATGACAGTATAAAAAATGTCCCATCTAGAC
ATTTGAGTGATAATAAAAAA

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MQIEKKDIRALSKDQLRDFFVANNDKAFRGNQVYEWLWSKGAHSFEDMTNVAKTTRSMLEENFVINHIKVDTMQRSNDGT
VKNAVRLHDGLVVESVLIPTETRTTACVSSQVGCSLDCNFCATARLKRMRNLEPGEIYDQIMAIDKESRLYHNHPLSNIV
FMGMGEPLMNYNNVIKAIDMVTSEEGLGMSPKRITLSTSGIPKMIKKMADDDVKFRLAVSLHSAIDETRAKIMPFSKNFP
LKDLREALEYWYRKTKSKVSYEYVVWKGINDDKASVDALVKFCKYVPCKVNLIEYNPIDDGEFQQASEESILAYIKALEN
IGVVVKVRRSRGKDIDAACGQLANKEAEV

Sequences:

>Translated_349_residues
MQIEKKDIRALSKDQLRDFFVANNDKAFRGNQVYEWLWSKGAHSFEDMTNVAKTTRSMLEENFVINHIKVDTMQRSNDGT
VKNAVRLHDGLVVESVLIPTETRTTACVSSQVGCSLDCNFCATARLKRMRNLEPGEIYDQIMAIDKESRLYHNHPLSNIV
FMGMGEPLMNYNNVIKAIDMVTSEEGLGMSPKRITLSTSGIPKMIKKMADDDVKFRLAVSLHSAIDETRAKIMPFSKNFP
LKDLREALEYWYRKTKSKVSYEYVVWKGINDDKASVDALVKFCKYVPCKVNLIEYNPIDDGEFQQASEESILAYIKALEN
IGVVVKVRRSRGKDIDAACGQLANKEAEV
>Mature_349_residues
MQIEKKDIRALSKDQLRDFFVANNDKAFRGNQVYEWLWSKGAHSFEDMTNVAKTTRSMLEENFVINHIKVDTMQRSNDGT
VKNAVRLHDGLVVESVLIPTETRTTACVSSQVGCSLDCNFCATARLKRMRNLEPGEIYDQIMAIDKESRLYHNHPLSNIV
FMGMGEPLMNYNNVIKAIDMVTSEEGLGMSPKRITLSTSGIPKMIKKMADDDVKFRLAVSLHSAIDETRAKIMPFSKNFP
LKDLREALEYWYRKTKSKVSYEYVVWKGINDDKASVDALVKFCKYVPCKVNLIEYNPIDDGEFQQASEESILAYIKALEN
IGVVVKVRRSRGKDIDAACGQLANKEAEV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=340, Percent_Identity=38.8235294117647, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_FLAJ1 (A5FJ06)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194133.1
- ProteinModelPortal:   A5FJ06
- STRING:   A5FJ06
- GeneID:   5091849
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1782
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   VQKYFDE
- ProtClustDB:   CLSK843592
- BioCyc:   FJOH376686:FJOH_1782-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39631; Mature: 39631

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIEKKDIRALSKDQLRDFFVANNDKAFRGNQVYEWLWSKGAHSFEDMTNVAKTTRSMLE
CCCCHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ENFVINHIKVDTMQRSNDGTVKNAVRLHDGLVVESVLIPTETRTTACVSSQVGCSLDCNF
HCCEEEEEEEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCH
CATARLKRMRNLEPGEIYDQIMAIDKESRLYHNHPLSNIVFMGMGEPLMNYNNVIKAIDM
HHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
VTSEEGLGMSPKRITLSTSGIPKMIKKMADDDVKFRLAVSLHSAIDETRAKIMPFSKNFP
HHCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCC
LKDLREALEYWYRKTKSKVSYEYVVWKGINDDKASVDALVKFCKYVPCKVNLIEYNPIDD
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
GEFQQASEESILAYIKALENIGVVVKVRRSRGKDIDAACGQLANKEAEV
CHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIEKKDIRALSKDQLRDFFVANNDKAFRGNQVYEWLWSKGAHSFEDMTNVAKTTRSMLE
CCCCHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ENFVINHIKVDTMQRSNDGTVKNAVRLHDGLVVESVLIPTETRTTACVSSQVGCSLDCNF
HCCEEEEEEEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCH
CATARLKRMRNLEPGEIYDQIMAIDKESRLYHNHPLSNIVFMGMGEPLMNYNNVIKAIDM
HHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
VTSEEGLGMSPKRITLSTSGIPKMIKKMADDDVKFRLAVSLHSAIDETRAKIMPFSKNFP
HHCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCC
LKDLREALEYWYRKTKSKVSYEYVVWKGINDDKASVDALVKFCKYVPCKVNLIEYNPIDD
HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
GEFQQASEESILAYIKALENIGVVVKVRRSRGKDIDAACGQLANKEAEV
CHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA