Click here to switch to the map view.

The map label for this gene is pdxJ

Identifier: 146299455

GI number: 146299455

Start: 1964223

End: 1964936

Strand: Direct

Name: pdxJ

Synonym: Fjoh_1695

Alternate gene names: 146299455

Gene position: 1964223-1964936 (Clockwise)

Preceding gene: 146299446

Following gene: 146299456

Centisome position: 32.22

GC content: 36.41

Gene sequence:

>714_bases
ATGACAAAGTTAAGTGTTAATATCAATAAAATTGCAACGCTGAGAAATGCTCGAGGCGGAAATGTTCCAGATTTATTAAA
GGTTGCTGCCGATATTCAGCGTTTTGGAGGGCAGGGAATTACGATTCACCCGCGTCCGGATGAGCGTCATATTCGTTATC
AGGATGCACGCGATTTAAAAGCAATTGTTACTACAGAATATAATATTGAAGGAAATCCGCAGCATAATTTTATTGATTTG
GTTTTAGAATGTAAACCGGATCAGGTTACTCTTGTTCCGGATGCAATTGGTGCTATTACATCTTCTGCCGGCTGGGATAC
TATTAAAAACCAAGAGTATTTAAAAGAAGTAATTCAGGAATTTCAAAGAAACGGAATCAGAACATCGATTTTTGTTGATC
CTGTTTTAGAAATGATCGAAGGTGCAAAAAAAACAGGTACAGACCGAATTGAATTGTATACCGAAGCTTTTGCACATCAA
TATGATTTGGGAAATAAAAACGGAATCGACCCGTATGTAAAAGCAGCTGAATTGGCAAACGAATTAGGTTTAGGAATTAA
TGCCGGCCACGATTTAAGTTTAGATAATATTCAGTTCTTTAAGCAGAACATTCCGGGATTATTAGAAGTTTCTATTGGAC
ACGCATTGATTTCTGAGGCACTTTACCTAGGTTTGGATAATGTGGTTAATATGTATTTGAAGAAATTGAAATAA

Upstream 100 bases:

>100_bases
TTTGATGCAAAATAATCAAAAAATACCAAAAACTGCTGCGTTTTACTTTGTATTTTTGTCGTAACAAATATAGTGTTACT
TGAATTAATTATCTATTTAT

Downstream 100 bases:

>100_bases
GATTAAAACTAAACAATTATAACCTGATGTATTTGGGTTATAATTGTTTACTTTCGCACTTTTCTTATTAAAATTTACGT
GTCAAAATCTGTTATACTCT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTKLSVNINKIATLRNARGGNVPDLLKVAADIQRFGGQGITIHPRPDERHIRYQDARDLKAIVTTEYNIEGNPQHNFIDL
VLECKPDQVTLVPDAIGAITSSAGWDTIKNQEYLKEVIQEFQRNGIRTSIFVDPVLEMIEGAKKTGTDRIELYTEAFAHQ
YDLGNKNGIDPYVKAAELANELGLGINAGHDLSLDNIQFFKQNIPGLLEVSIGHALISEALYLGLDNVVNMYLKKLK

Sequences:

>Translated_237_residues
MTKLSVNINKIATLRNARGGNVPDLLKVAADIQRFGGQGITIHPRPDERHIRYQDARDLKAIVTTEYNIEGNPQHNFIDL
VLECKPDQVTLVPDAIGAITSSAGWDTIKNQEYLKEVIQEFQRNGIRTSIFVDPVLEMIEGAKKTGTDRIELYTEAFAHQ
YDLGNKNGIDPYVKAAELANELGLGINAGHDLSLDNIQFFKQNIPGLLEVSIGHALISEALYLGLDNVVNMYLKKLK
>Mature_236_residues
TKLSVNINKIATLRNARGGNVPDLLKVAADIQRFGGQGITIHPRPDERHIRYQDARDLKAIVTTEYNIEGNPQHNFIDLV
LECKPDQVTLVPDAIGAITSSAGWDTIKNQEYLKEVIQEFQRNGIRTSIFVDPVLEMIEGAKKTGTDRIELYTEAFAHQY
DLGNKNGIDPYVKAAELANELGLGINAGHDLSLDNIQFFKQNIPGLLEVSIGHALISEALYLGLDNVVNMYLKKLK

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=227, Percent_Identity=35.2422907488987, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_FLAJ1 (A5FJ95)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194046.1
- ProteinModelPortal:   A5FJ95
- SMR:   A5FJ95
- STRING:   A5FJ95
- GeneID:   5091154
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1695
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   FJOH376686:FJOH_1695-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26351; Mature: 26220

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 190-190 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 100-100 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSVNINKIATLRNARGGNVPDLLKVAADIQRFGGQGITIHPRPDERHIRYQDARDLK
CCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCE
AIVTTEYNIEGNPQHNFIDLVLECKPDQVTLVPDAIGAITSSAGWDTIKNQEYLKEVIQE
EEEEEEECCCCCCCCCEEEEEEECCCCCEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHH
FQRNGIRTSIFVDPVLEMIEGAKKTGTDRIELYTEAFAHQYDLGNKNGIDPYVKAAELAN
HHHCCCEEEEHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ELGLGINAGHDLSLDNIQFFKQNIPGLLEVSIGHALISEALYLGLDNVVNMYLKKLK
HHCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKLSVNINKIATLRNARGGNVPDLLKVAADIQRFGGQGITIHPRPDERHIRYQDARDLK
CEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCE
AIVTTEYNIEGNPQHNFIDLVLECKPDQVTLVPDAIGAITSSAGWDTIKNQEYLKEVIQE
EEEEEEECCCCCCCCCEEEEEEECCCCCEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHH
FQRNGIRTSIFVDPVLEMIEGAKKTGTDRIELYTEAFAHQYDLGNKNGIDPYVKAAELAN
HHHCCCEEEEHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
ELGLGINAGHDLSLDNIQFFKQNIPGLLEVSIGHALISEALYLGLDNVVNMYLKKLK
HHCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA