Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 146299389

GI number: 146299389

Start: 1880058

End: 1881311

Strand: Direct

Name: nusA [H]

Synonym: Fjoh_1629

Alternate gene names: 146299389

Gene position: 1880058-1881311 (Clockwise)

Preceding gene: 146299388

Following gene: 146299390

Centisome position: 30.84

GC content: 33.73

Gene sequence:

>1254_bases
ATGGAAAATTTAGCATTAATCGATTCATTCTCAGAGTTTAAAGATAATAAACTTATTGATCGTGTAACGCTTATGGCAAT
TTTGGAGGACGTGTTTAGAAATGCACTAAAGAAAAAATACGGTTCTGATGATAATTTCGATATCATTATAAATCCTGATA
AAGGAGATATGGAGATTTGGAGAAGAAGAGTTATCGTTGCTGATGAAGATCTGGACTTTGAGAACGAAGAAATTACTTTG
ACTGAAGCAAGGATGATCGAGGCGGATTTTGAAATTGGTGAGGAAGTTTCTGAAGAGGTTAAATTAATTGACTTAGGAAG
AAGAGCTATTTTGGCTTTGCGTCAAAACTTAATATCTAAAATTCACGAACACGATAATACAAATCTTTATAAACAATTTA
AAGATATTATTGGTGATATTTATACTGCCGAAGTGCACCACGTTCGCCCAAGAGTTGTTATCTTAGTAGACGATGAAGGG
AATGAGATTGTGCTTCCAAAAGAAAAACAAATTCCTTCTGACTTTTTCCGTAAAGGAGATAATGTACGCGGAATTATTGA
AAGCGTTGAATTAAAAGGGAACAAACCTCAAATTATTATGTCTAGAACTTCTGAGAAGTTTTTAGAAAAATTATTCGAGC
AGGAAATTCCAGAAGTATTCGACGGCTTAATTACAGTTAAAAATGTAGTTCGTATTCCGGGAGAAAAAGCAAAAGTTGCT
GTAGATTCGTATGATGACAGAATTGATCCTGTTGGAGCCTGCGTAGGTATGAAGGGATCTCGTATTCATGGAATTGTACG
TGAGTTAGGAAATGAAAATATTGATGTAATCAATTATACAAACAATATTCAATTGTTCATTACAAGAGCATTAAGCCCTG
CAAAAGTTTCTTCAATCAAAATAGATGAAGAAAGTAAAAGAGCTGAAGTTTTCTTGAAATTAGAAGAAGTTTCTAAAGCA
ATTGGTAGAGGCGGACATAATATTAAATTAGCGGGTCAATTAACAGGGTATGAGCTGGATGTTATTCGTGAAGGAGATGT
TGCTGGTACAGTTGCAGATGAAGACGATGTTGAATTGACAGAGTTTTCAGATGAAATCGAAGAATGGGTAATTGAAGAGT
TTGCTAAAATAGGTTTAGATACTGCAAAAAGTATCTTGAAACACGACGTAGAAGATTTAGTAAGAAGAACAGACTTAGAA
GAGGAAACAATTCTAGATGTTATGAAAATACTAAAAGAAGAGTTTGATAGTTAA

Upstream 100 bases:

>100_bases
GTAGGAAAAGGAAAAGAAACAGTTCAAAAAGAACAACAGATACCTTATACAGAGATAAAAGAGGCAGTTGTTACAGTAAC
ATTTTAATTAAAGAATTCGC

Downstream 100 bases:

>100_bases
GCAATAGCTTTATCTGCTCTAACAACACAACTAAAAAAGGTAATAATAAAAAGGTTATATGTCTGAAGAGAGAGTAATAA
GAATAAATAAGGTTTTAAGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MENLALIDSFSEFKDNKLIDRVTLMAILEDVFRNALKKKYGSDDNFDIIINPDKGDMEIWRRRVIVADEDLDFENEEITL
TEARMIEADFEIGEEVSEEVKLIDLGRRAILALRQNLISKIHEHDNTNLYKQFKDIIGDIYTAEVHHVRPRVVILVDDEG
NEIVLPKEKQIPSDFFRKGDNVRGIIESVELKGNKPQIIMSRTSEKFLEKLFEQEIPEVFDGLITVKNVVRIPGEKAKVA
VDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDVINYTNNIQLFITRALSPAKVSSIKIDEESKRAEVFLKLEEVSKA
IGRGGHNIKLAGQLTGYELDVIREGDVAGTVADEDDVELTEFSDEIEEWVIEEFAKIGLDTAKSILKHDVEDLVRRTDLE
EETILDVMKILKEEFDS

Sequences:

>Translated_417_residues
MENLALIDSFSEFKDNKLIDRVTLMAILEDVFRNALKKKYGSDDNFDIIINPDKGDMEIWRRRVIVADEDLDFENEEITL
TEARMIEADFEIGEEVSEEVKLIDLGRRAILALRQNLISKIHEHDNTNLYKQFKDIIGDIYTAEVHHVRPRVVILVDDEG
NEIVLPKEKQIPSDFFRKGDNVRGIIESVELKGNKPQIIMSRTSEKFLEKLFEQEIPEVFDGLITVKNVVRIPGEKAKVA
VDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDVINYTNNIQLFITRALSPAKVSSIKIDEESKRAEVFLKLEEVSKA
IGRGGHNIKLAGQLTGYELDVIREGDVAGTVADEDDVELTEFSDEIEEWVIEEFAKIGLDTAKSILKHDVEDLVRRTDLE
EETILDVMKILKEEFDS
>Mature_417_residues
MENLALIDSFSEFKDNKLIDRVTLMAILEDVFRNALKKKYGSDDNFDIIINPDKGDMEIWRRRVIVADEDLDFENEEITL
TEARMIEADFEIGEEVSEEVKLIDLGRRAILALRQNLISKIHEHDNTNLYKQFKDIIGDIYTAEVHHVRPRVVILVDDEG
NEIVLPKEKQIPSDFFRKGDNVRGIIESVELKGNKPQIIMSRTSEKFLEKLFEQEIPEVFDGLITVKNVVRIPGEKAKVA
VDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDVINYTNNIQLFITRALSPAKVSSIKIDEESKRAEVFLKLEEVSKA
IGRGGHNIKLAGQLTGYELDVIREGDVAGTVADEDDVELTEFSDEIEEWVIEEFAKIGLDTAKSILKHDVEDLVRRTDLE
EETILDVMKILKEEFDS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=409, Percent_Identity=31.2958435207824, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 47500; Mature: 47500

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLALIDSFSEFKDNKLIDRVTLMAILEDVFRNALKKKYGSDDNFDIIINPDKGDMEIW
CCCCHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHH
RRRVIVADEDLDFENEEITLTEARMIEADFEIGEEVSEEVKLIDLGRRAILALRQNLISK
HCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHEHDNTNLYKQFKDIIGDIYTAEVHHVRPRVVILVDDEGNEIVLPKEKQIPSDFFRKGD
HHHCCCCHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCEEECCCCCCCCHHHHHCCC
NVRGIIESVELKGNKPQIIMSRTSEKFLEKLFEQEIPEVFDGLITVKNVVRIPGEKAKVA
CHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDVINYTNNIQLFITRALSPAKVSSIK
ECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCHHCEEE
IDEESKRAEVFLKLEEVSKAIGRGGHNIKLAGQLTGYELDVIREGDVAGTVADEDDVELT
ECCCCCHHHHHEEHHHHHHHHHCCCCEEEEEEEECCEEEEEEECCCCCEECCCCCCCHHH
EFSDEIEEWVIEEFAKIGLDTAKSILKHDVEDLVRRTDLEEETILDVMKILKEEFDS
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENLALIDSFSEFKDNKLIDRVTLMAILEDVFRNALKKKYGSDDNFDIIINPDKGDMEIW
CCCCHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHH
RRRVIVADEDLDFENEEITLTEARMIEADFEIGEEVSEEVKLIDLGRRAILALRQNLISK
HCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHEHDNTNLYKQFKDIIGDIYTAEVHHVRPRVVILVDDEGNEIVLPKEKQIPSDFFRKGD
HHHCCCCHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCEEECCCCCCCCHHHHHCCC
NVRGIIESVELKGNKPQIIMSRTSEKFLEKLFEQEIPEVFDGLITVKNVVRIPGEKAKVA
CHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDVINYTNNIQLFITRALSPAKVSSIK
ECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCHHCEEE
IDEESKRAEVFLKLEEVSKAIGRGGHNIKLAGQLTGYELDVIREGDVAGTVADEDDVELT
ECCCCCHHHHHEEHHHHHHHHHCCCCEEEEEEEECCEEEEEEECCCCCEECCCCCCCHHH
EFSDEIEEWVIEEFAKIGLDTAKSILKHDVEDLVRRTDLEEETILDVMKILKEEFDS
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]