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The map label for this gene is metH [H]

Identifier: 146299270

GI number: 146299270

Start: 1739239

End: 1741914

Strand: Direct

Name: metH [H]

Synonym: Fjoh_1510

Alternate gene names: 146299270

Gene position: 1739239-1741914 (Clockwise)

Preceding gene: 146299269

Following gene: 146299271

Centisome position: 28.53

GC content: 38.9

Gene sequence:

>2676_bases
ATGACAGAAAAAAGAAGAGACCTTGTATTATCAGGATTAGAACCGTTAATTATTACGCCGGAAAGTGTTTTCGTGAATGT
TGGTGAGCGTACAAACGTAACAGGTTCAAGAAAATTCTTGAGATTAATCAAGGAAGAGAAATATGACGAGGCGCTTGATA
TTGCAAGACAACAAGTAGAAAGCGGGGCACAGATCATCGATATTAATATGGATGAAGGAATGCTTGACGGAGTTAATGCA
ATGACTAAATTTTTGAATTTAATTGCTGCAGAACCAGACATTTCGAGAGTACCGATTATGATCGACAGTTCGAAATGGGA
AATCATTGAAGCAGGTCTAAAAGTAGTACAAGGAAAAAGCGTTGTAAACTCGATTTCGTTAAAAGAAGGGGAAGAAGCCT
TTATTCACCACGCCAAATTAATCAAACGTTACGGAGCTGCTGCTATTGTAATGGCTTTTGATGAAGTGGGTCAGGCCGAT
AATTACGATCGTAGAGTTGAAATTTGCCAGCGTTCGTATGATATTTTGGTGAACAAAGTAAATTTCCCTCCACAAGATAT
TATTTTCGATTTAAATATTTTCCCAGTTGCAACGGGAATGGAAGAGCATAGATTGAACGCTCTGGATTTCTTTAGAGGAA
CAAAATGGGTTCGTGAAAATCTTCCTCATGCGCACATCAGCGGTGGAGTAAGTAACGTTTCGTTCTCGTTTAGAGGGAAT
GATGTTGTTCGTGAAGCAATGCACTCGGTGTTTTTATACCACGCAATTAAAGAAGGAATGACGATGGGAATCGTTAATCC
GGAGATGTTGACGATTTACGATGACATTCCGAAAGATTTATTAGAACACGTTGAAGACGTAATTTTAGATAGACGTGATG
ATGCCACAGAACGACTTTTGGACTTTGCAGAAAATGTAAAAGGCGAAGCAAAGAGCGATGAAAAAGTAGTTCAGGAATGG
CGTTTTGGAACTGTTCAGGATCGTATTACACATTCGTTGGTAAAAGGAATCGATGCTTTTATTGAAGAAGATGTTGAAGA
AGCACGTTTGGCAGCAACAAAACCAATCGAAGTTATCGAAATCAATTTGATGACGGGAATGAATGTAGTTGGAGATTTAT
TCGGAAGCGGAAAAATGTTCCTGCCTCAGGTAGTAAAGTCGGCTCGTGTAATGAAAAAAGCCGTGGCTTATTTATTGCCA
TTTATCGAAGCGAGTAAACAAGCAGGGGACAAACAAGGAAACGGAAAAATCTTGATGGCAACCGTAAAAGGTGACGTTCA
CGATATTGGTAAAAACATCGTTTCTGTGGTTTTGGCTTGTAATAATTATGAGATTGTAGATCTTGGTGTTATGGTGCCTC
CGGAAAAAATCATTGCAGCGGCGATTGAACATGAAGTAGATATTATTGGATTAAGTGGATTGATTACGCCTTCGCTTGAC
GAAATGGTTTATTTAGCCAAAGAATTAGACAAACAAGATATTAAAATCCCGATTATGATTGGAGGAGCAACAACTTCGCG
TGCACATACTGCCGTGAAAATTGCACCTCAGTACAGAGAAACGGTAATTCACGTAAACGATGCTTCGAGAGCCGTTACCG
TTGCAGGAAATCTGTTAGATCATAATAGAAAAATATATGCGGCAGATATTCGTGCAGAATACGATTCGTTTAGAGAAACG
TTCTTAAATCGTTCGAGAGATAAAAACTTCCTGACGATTGAAGATGCCCGCAAAAACAAATTGCCATTGGATTGGTCGGA
ATACACGCCAACTAAGCCAAAAGTAATCGGTGCACAAACCATCGAAATTGAACTAGATGTTTTGGTTCCATATATCGACT
GGACGCCATTTTTTCAGACTTGGGAATTGTACGGAAAATATCCAGCAATTTTAACGGATGAGGTTGTGGGTAAAGAAGCA
ACTTCTGTTTTTAACGATGCTCAGGCAATGTTGAAAGTGATTTTAGAAGAGAAAAAATTAAAAGCAAAAGGTATTTACGG
AATTTTCCCTGCCAATCAGGTAAATGATGACGATATCGAATTGCGTGATGAAAACGGAAAAGTTTTAGAAAAATTCTTAA
CGCTTCGTCAGCAGTCACAAAAAACAAAAGGAGCTCCAAACATCGCTTTAGCTGATTTTATTCTGCCAAAAGAAAGCGGA
ATAGAAGATTATATTGGAGCTTTTTGTGTAACAACAGGTTTCGGTGTAGACGAATGGGCGGCTGAATATGAAAAGAATTT
AGACGATTACAATTCGATTATGGTAAAAGCGCTTGCCGATCGTTTTGCTGAGGCTTTCGCCGAATACCTTCATGAGAGAG
TACGTAAAGATTTCTGGGGTTATGATGTCGATGAATCTTTAACCAACGAAGAATTGATTAAAGAAAGTTATAAAGGAATT
CGTCCAGCTCCGGGTTATCCAGCTTGTCCAGATCACTTGGAAAAACCAACAATCTGGAAGCTTTTAGATGTTGAAAAACA
AATAGGAGTGAAACTAACAGAAAGCATGGCAATGTGGCCGGCTTCTTCGGTTTCAGGATATTATTTCGGAAACCCAAAAA
GCCGCTATTTCGGACTCGGAAAAATAAAAGAAGATCAAGTTACAGATTACGCAAAACGCCGAAATGTACCGCTTGATTAC
GCAATGAAGTGGTTAAACCCTAATATAGCAGATTAA

Upstream 100 bases:

>100_bases
GAAAACATAAATACCTGTACGGTTTTAAAAACCTTACAGGGAAAATAGAATAAAAAACTTAGAACCTTAGCATCTTAGGA
TCTCAGTAACTTTAAAAAAA

Downstream 100 bases:

>100_bases
ATTAGTCGAAAGTCAAAAGTCGTAAAGTCCGAAAGGAAAGACTTTAGACTTTTGACTTTACAACTTTAAGACTTAATAAT
GAAAGTAACAGAACATATAG

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 891; Mature: 890

Protein sequence:

>891_residues
MTEKRRDLVLSGLEPLIITPESVFVNVGERTNVTGSRKFLRLIKEEKYDEALDIARQQVESGAQIIDINMDEGMLDGVNA
MTKFLNLIAAEPDISRVPIMIDSSKWEIIEAGLKVVQGKSVVNSISLKEGEEAFIHHAKLIKRYGAAAIVMAFDEVGQAD
NYDRRVEICQRSYDILVNKVNFPPQDIIFDLNIFPVATGMEEHRLNALDFFRGTKWVRENLPHAHISGGVSNVSFSFRGN
DVVREAMHSVFLYHAIKEGMTMGIVNPEMLTIYDDIPKDLLEHVEDVILDRRDDATERLLDFAENVKGEAKSDEKVVQEW
RFGTVQDRITHSLVKGIDAFIEEDVEEARLAATKPIEVIEINLMTGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLP
FIEASKQAGDKQGNGKILMATVKGDVHDIGKNIVSVVLACNNYEIVDLGVMVPPEKIIAAAIEHEVDIIGLSGLITPSLD
EMVYLAKELDKQDIKIPIMIGGATTSRAHTAVKIAPQYRETVIHVNDASRAVTVAGNLLDHNRKIYAADIRAEYDSFRET
FLNRSRDKNFLTIEDARKNKLPLDWSEYTPTKPKVIGAQTIEIELDVLVPYIDWTPFFQTWELYGKYPAILTDEVVGKEA
TSVFNDAQAMLKVILEEKKLKAKGIYGIFPANQVNDDDIELRDENGKVLEKFLTLRQQSQKTKGAPNIALADFILPKESG
IEDYIGAFCVTTGFGVDEWAAEYEKNLDDYNSIMVKALADRFAEAFAEYLHERVRKDFWGYDVDESLTNEELIKESYKGI
RPAPGYPACPDHLEKPTIWKLLDVEKQIGVKLTESMAMWPASSVSGYYFGNPKSRYFGLGKIKEDQVTDYAKRRNVPLDY
AMKWLNPNIAD

Sequences:

>Translated_891_residues
MTEKRRDLVLSGLEPLIITPESVFVNVGERTNVTGSRKFLRLIKEEKYDEALDIARQQVESGAQIIDINMDEGMLDGVNA
MTKFLNLIAAEPDISRVPIMIDSSKWEIIEAGLKVVQGKSVVNSISLKEGEEAFIHHAKLIKRYGAAAIVMAFDEVGQAD
NYDRRVEICQRSYDILVNKVNFPPQDIIFDLNIFPVATGMEEHRLNALDFFRGTKWVRENLPHAHISGGVSNVSFSFRGN
DVVREAMHSVFLYHAIKEGMTMGIVNPEMLTIYDDIPKDLLEHVEDVILDRRDDATERLLDFAENVKGEAKSDEKVVQEW
RFGTVQDRITHSLVKGIDAFIEEDVEEARLAATKPIEVIEINLMTGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLP
FIEASKQAGDKQGNGKILMATVKGDVHDIGKNIVSVVLACNNYEIVDLGVMVPPEKIIAAAIEHEVDIIGLSGLITPSLD
EMVYLAKELDKQDIKIPIMIGGATTSRAHTAVKIAPQYRETVIHVNDASRAVTVAGNLLDHNRKIYAADIRAEYDSFRET
FLNRSRDKNFLTIEDARKNKLPLDWSEYTPTKPKVIGAQTIEIELDVLVPYIDWTPFFQTWELYGKYPAILTDEVVGKEA
TSVFNDAQAMLKVILEEKKLKAKGIYGIFPANQVNDDDIELRDENGKVLEKFLTLRQQSQKTKGAPNIALADFILPKESG
IEDYIGAFCVTTGFGVDEWAAEYEKNLDDYNSIMVKALADRFAEAFAEYLHERVRKDFWGYDVDESLTNEELIKESYKGI
RPAPGYPACPDHLEKPTIWKLLDVEKQIGVKLTESMAMWPASSVSGYYFGNPKSRYFGLGKIKEDQVTDYAKRRNVPLDY
AMKWLNPNIAD
>Mature_890_residues
TEKRRDLVLSGLEPLIITPESVFVNVGERTNVTGSRKFLRLIKEEKYDEALDIARQQVESGAQIIDINMDEGMLDGVNAM
TKFLNLIAAEPDISRVPIMIDSSKWEIIEAGLKVVQGKSVVNSISLKEGEEAFIHHAKLIKRYGAAAIVMAFDEVGQADN
YDRRVEICQRSYDILVNKVNFPPQDIIFDLNIFPVATGMEEHRLNALDFFRGTKWVRENLPHAHISGGVSNVSFSFRGND
VVREAMHSVFLYHAIKEGMTMGIVNPEMLTIYDDIPKDLLEHVEDVILDRRDDATERLLDFAENVKGEAKSDEKVVQEWR
FGTVQDRITHSLVKGIDAFIEEDVEEARLAATKPIEVIEINLMTGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPF
IEASKQAGDKQGNGKILMATVKGDVHDIGKNIVSVVLACNNYEIVDLGVMVPPEKIIAAAIEHEVDIIGLSGLITPSLDE
MVYLAKELDKQDIKIPIMIGGATTSRAHTAVKIAPQYRETVIHVNDASRAVTVAGNLLDHNRKIYAADIRAEYDSFRETF
LNRSRDKNFLTIEDARKNKLPLDWSEYTPTKPKVIGAQTIEIELDVLVPYIDWTPFFQTWELYGKYPAILTDEVVGKEAT
SVFNDAQAMLKVILEEKKLKAKGIYGIFPANQVNDDDIELRDENGKVLEKFLTLRQQSQKTKGAPNIALADFILPKESGI
EDYIGAFCVTTGFGVDEWAAEYEKNLDDYNSIMVKALADRFAEAFAEYLHERVRKDFWGYDVDESLTNEELIKESYKGIR
PAPGYPACPDHLEKPTIWKLLDVEKQIGVKLTESMAMWPASSVSGYYFGNPKSRYFGLGKIKEDQVTDYAKRRNVPLDYA
MKWLNPNIAD

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=909, Percent_Identity=54.1254125412541, Blast_Score=973, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=885, Percent_Identity=62.5988700564972, Blast_Score=1099, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=900, Percent_Identity=54, Blast_Score=965, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 100238; Mature: 100107

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50006 FHA_DOMAIN ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKRRDLVLSGLEPLIITPESVFVNVGERTNVTGSRKFLRLIKEEKYDEALDIARQQVE
CCCHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAQIIDINMDEGMLDGVNAMTKFLNLIAAEPDISRVPIMIDSSKWEIIEAGLKVVQGKS
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCHH
VVNSISLKEGEEAFIHHAKLIKRYGAAAIVMAFDEVGQADNYDRRVEICQRSYDILVNKV
HHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHEEEEC
NFPPQDIIFDLNIFPVATGMEEHRLNALDFFRGTKWVRENLPHAHISGGVSNVSFSFRGN
CCCCHHEEEEEEEEEEECCCHHHHCHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEECCC
DVVREAMHSVFLYHAIKEGMTMGIVNPEMLTIYDDIPKDLLEHVEDVILDRRDDATERLL
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DFAENVKGEAKSDEKVVQEWRFGTVQDRITHSLVKGIDAFIEEDVEEARLAATKPIEVIE
HHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
INLMTGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFIEASKQAGDKQGNGKILMA
EEEHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
TVKGDVHDIGKNIVSVVLACNNYEIVDLGVMVPPEKIIAAAIEHEVDIIGLSGLITPSLD
EECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCHH
EMVYLAKELDKQDIKIPIMIGGATTSRAHTAVKIAPQYRETVIHVNDASRAVTVAGNLLD
HHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEECHHHH
HNRKIYAADIRAEYDSFRETFLNRSRDKNFLTIEDARKNKLPLDWSEYTPTKPKVIGAQT
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEECCEE
IEIELDVLVPYIDWTPFFQTWELYGKYPAILTDEVVGKEATSVFNDAQAMLKVILEEKKL
EEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KAKGIYGIFPANQVNDDDIELRDENGKVLEKFLTLRQQSQKTKGAPNIALADFILPKESG
HHCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEHEECCCCCC
IEDYIGAFCVTTGFGVDEWAAEYEKNLDDYNSIMVKALADRFAEAFAEYLHERVRKDFWG
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YDVDESLTNEELIKESYKGIRPAPGYPACPDHLEKPTIWKLLDVEKQIGVKLTESMAMWP
CCCCHHCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCEEHHHHHHCC
ASSVSGYYFGNPKSRYFGLGKIKEDQVTDYAKRRNVPLDYAMKWLNPNIAD
CCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEKRRDLVLSGLEPLIITPESVFVNVGERTNVTGSRKFLRLIKEEKYDEALDIARQQVE
CCHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAQIIDINMDEGMLDGVNAMTKFLNLIAAEPDISRVPIMIDSSKWEIIEAGLKVVQGKS
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCHH
VVNSISLKEGEEAFIHHAKLIKRYGAAAIVMAFDEVGQADNYDRRVEICQRSYDILVNKV
HHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHEEEEC
NFPPQDIIFDLNIFPVATGMEEHRLNALDFFRGTKWVRENLPHAHISGGVSNVSFSFRGN
CCCCHHEEEEEEEEEEECCCHHHHCHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEECCC
DVVREAMHSVFLYHAIKEGMTMGIVNPEMLTIYDDIPKDLLEHVEDVILDRRDDATERLL
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DFAENVKGEAKSDEKVVQEWRFGTVQDRITHSLVKGIDAFIEEDVEEARLAATKPIEVIE
HHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
INLMTGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFIEASKQAGDKQGNGKILMA
EEEHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
TVKGDVHDIGKNIVSVVLACNNYEIVDLGVMVPPEKIIAAAIEHEVDIIGLSGLITPSLD
EECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCHH
EMVYLAKELDKQDIKIPIMIGGATTSRAHTAVKIAPQYRETVIHVNDASRAVTVAGNLLD
HHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEECHHHH
HNRKIYAADIRAEYDSFRETFLNRSRDKNFLTIEDARKNKLPLDWSEYTPTKPKVIGAQT
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEECCEE
IEIELDVLVPYIDWTPFFQTWELYGKYPAILTDEVVGKEATSVFNDAQAMLKVILEEKKL
EEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KAKGIYGIFPANQVNDDDIELRDENGKVLEKFLTLRQQSQKTKGAPNIALADFILPKESG
HHCCEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEHEECCCCCC
IEDYIGAFCVTTGFGVDEWAAEYEKNLDDYNSIMVKALADRFAEAFAEYLHERVRKDFWG
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YDVDESLTNEELIKESYKGIRPAPGYPACPDHLEKPTIWKLLDVEKQIGVKLTESMAMWP
CCCCHHCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCEEHHHHHHCC
ASSVSGYYFGNPKSRYFGLGKIKEDQVTDYAKRRNVPLDYAMKWLNPNIAD
CCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]