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The map label for this gene is metF [H]

Identifier: 146299271

GI number: 146299271

Start: 1741993

End: 1742949

Strand: Direct

Name: metF [H]

Synonym: Fjoh_1511

Alternate gene names: 146299271

Gene position: 1741993-1742949 (Clockwise)

Preceding gene: 146299270

Following gene: 146299272

Centisome position: 28.57

GC content: 36.47

Gene sequence:

>957_bases
ATGAAAGTAACAGAACATATAGAAAACGCCAAAGGAAAAACATTATTCTCATTTGAAATTATTCCGCCTCAAAAGGGAAA
AAGCATTCAGGAATTATACGATAATATTGATCCGTTAATGGAGTTTAATCCGCCGTTTATTGATGTAACGACTTCACGTG
AGGAGTATATTTACATTGATAAAGGCAACGGACTTTTGGACAAAAAACTGACTCGTATGCGTCCTGGAACGCTTGGAATC
TGCGCTTCTATAAAACATAAATACAATGTAGACACTGTGCCGCATTTGCTTTGCGGTGGTTTTACCAAAGAAGAAACCGA
ATACATGCTGGTTGACTGTCATTATTTAGGAATTAACAACGTAATGGCGCTTCGCGGCGATGCAATGAAAGATGAACAAT
CTTTTGTGCCCAAAGCTGGCGGTAACCATTATGCAATTGATTTGGTTCGTCAAATTAAAGATCTAAACTGCGGGCAATAT
CTGCATGAAGTAATAGATGCTGATAACAAAGCAGATTTCTGTATTGGAGTTGCCGGTTATCCGGAAAAACATTTAGAATC
ACCATCTTTACAATCAGATTTAAAAAGATTAAAAGAAAAAGTAGATGCAGGAGCAGATTATGTGGTAACACAAATGTTTT
TTGACAATTCTAAATATTTTGCTTTTGTAGAAAAAGCAAGAGAAATGGGAATCACAATTCCGATTATTCCTGGAATTAAA
CCCATTGCGGTTCAGAGACATTTACAGGTTTTACCACAGATTTTCAGAATCGATTTACCAGAAGATTTAATCGATGCTGT
AGATAAATGCAAAAACAACGCTGAAATCAAACAAGTCGGAATCGAATGGGCGATTCAACAGTCATTAGAATTAAAAGCCG
CAGGAGTTCCATTTTTACACTATTATTCAATGGGTAAGTCTGAGAATATCCGCCAGATTGCAAGTCAGGTTTTTTAG

Upstream 100 bases:

>100_bases
AAACCCTAATATAGCAGATTAAATTAGTCGAAAGTCAAAAGTCGTAAAGTCCGAAAGGAAAGACTTTAGACTTTTGACTT
TACAACTTTAAGACTTAATA

Downstream 100 bases:

>100_bases
TTTTTTTTACCATTAAGAGATTAAGTTCATAAAGCCAGGCCTTAATTTTCTTTTTACCATTTAGAATTAAGCTCATCAAG
TTTAGCTTAATTTTCTTAAT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKVTEHIENAKGKTLFSFEIIPPQKGKSIQELYDNIDPLMEFNPPFIDVTTSREEYIYIDKGNGLLDKKLTRMRPGTLGI
CASIKHKYNVDTVPHLLCGGFTKEETEYMLVDCHYLGINNVMALRGDAMKDEQSFVPKAGGNHYAIDLVRQIKDLNCGQY
LHEVIDADNKADFCIGVAGYPEKHLESPSLQSDLKRLKEKVDAGADYVVTQMFFDNSKYFAFVEKAREMGITIPIIPGIK
PIAVQRHLQVLPQIFRIDLPEDLIDAVDKCKNNAEIKQVGIEWAIQQSLELKAAGVPFLHYYSMGKSENIRQIASQVF

Sequences:

>Translated_318_residues
MKVTEHIENAKGKTLFSFEIIPPQKGKSIQELYDNIDPLMEFNPPFIDVTTSREEYIYIDKGNGLLDKKLTRMRPGTLGI
CASIKHKYNVDTVPHLLCGGFTKEETEYMLVDCHYLGINNVMALRGDAMKDEQSFVPKAGGNHYAIDLVRQIKDLNCGQY
LHEVIDADNKADFCIGVAGYPEKHLESPSLQSDLKRLKEKVDAGADYVVTQMFFDNSKYFAFVEKAREMGITIPIIPGIK
PIAVQRHLQVLPQIFRIDLPEDLIDAVDKCKNNAEIKQVGIEWAIQQSLELKAAGVPFLHYYSMGKSENIRQIASQVF
>Mature_318_residues
MKVTEHIENAKGKTLFSFEIIPPQKGKSIQELYDNIDPLMEFNPPFIDVTTSREEYIYIDKGNGLLDKKLTRMRPGTLGI
CASIKHKYNVDTVPHLLCGGFTKEETEYMLVDCHYLGINNVMALRGDAMKDEQSFVPKAGGNHYAIDLVRQIKDLNCGQY
LHEVIDADNKADFCIGVAGYPEKHLESPSLQSDLKRLKEKVDAGADYVVTQMFFDNSKYFAFVEKAREMGITIPIIPGIK
PIAVQRHLQVLPQIFRIDLPEDLIDAVDKCKNNAEIKQVGIEWAIQQSLELKAAGVPFLHYYSMGKSENIRQIASQVF

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=297, Percent_Identity=35.3535353535354, Blast_Score=168, Evalue=5e-42,
Organism=Escherichia coli, GI1790377, Length=309, Percent_Identity=30.42071197411, Blast_Score=138, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25147436, Length=308, Percent_Identity=30.1948051948052, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI25147441, Length=308, Percent_Identity=30.1948051948052, Blast_Score=140, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6321313, Length=313, Percent_Identity=30.0319488817891, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6325234, Length=329, Percent_Identity=28.8753799392097, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 35959; Mature: 35959

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTEHIENAKGKTLFSFEIIPPQKGKSIQELYDNIDPLMEFNPPFIDVTTSREEYIYID
CCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEE
KGNGLLDKKLTRMRPGTLGICASIKHKYNVDTVPHLLCGGFTKEETEYMLVDCHYLGINN
CCCCCHHHHHHHCCCCCEEHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEECCCC
VMALRGDAMKDEQSFVPKAGGNHYAIDLVRQIKDLNCGQYLHEVIDADNKADFCIGVAGY
EEEECCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCC
PEKHLESPSLQSDLKRLKEKVDAGADYVVTQMFFDNSKYFAFVEKAREMGITIPIIPGIK
CHHHCCCCCHHHHHHHHHHHHCCCCCCEEEHEECCCCCHHHHHHHHHHCCCEEEECCCCC
PIAVQRHLQVLPQIFRIDLPEDLIDAVDKCKNNAEIKQVGIEWAIQQSLELKAAGVPFLH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
YYSMGKSENIRQIASQVF
EECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKVTEHIENAKGKTLFSFEIIPPQKGKSIQELYDNIDPLMEFNPPFIDVTTSREEYIYID
CCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEE
KGNGLLDKKLTRMRPGTLGICASIKHKYNVDTVPHLLCGGFTKEETEYMLVDCHYLGINN
CCCCCHHHHHHHCCCCCEEHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEECCCC
VMALRGDAMKDEQSFVPKAGGNHYAIDLVRQIKDLNCGQYLHEVIDADNKADFCIGVAGY
EEEECCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCC
PEKHLESPSLQSDLKRLKEKVDAGADYVVTQMFFDNSKYFAFVEKAREMGITIPIIPGIK
CHHHCCCCCHHHHHHHHHHHHCCCCCCEEEHEECCCCCHHHHHHHHHHCCCEEEECCCCC
PIAVQRHLQVLPQIFRIDLPEDLIDAVDKCKNNAEIKQVGIEWAIQQSLELKAAGVPFLH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
YYSMGKSENIRQIASQVF
EECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]