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The map label for this gene is pckA

Identifier: 146299126

GI number: 146299126

Start: 1562147

End: 1563751

Strand: Direct

Name: pckA

Synonym: Fjoh_1366

Alternate gene names: 146299126

Gene position: 1562147-1563751 (Clockwise)

Preceding gene: 146299125

Following gene: 146299129

Centisome position: 25.62

GC content: 37.2

Gene sequence:

>1605_bases
ATGGACAATAACACACTTTTCGCGCAATCGATTTCGTTAAAAGAATTAGGAATAGAAAATGCAACGGTTCGTTACCAGTT
ATCAGCAGATGAGCTTCATGCAATAACTGTGCAGTCAGGACAGGGTGTTGAGGCCTCTACAGGTGCTTTGGCAATTAATA
CAGGTGAATTTACAGGACGTTCTCCACAAGATCGTTTTATTGTAAAAGACAGCATTACAGAAGATAAAGTATGGTGGGGA
AATGTAAATATCCCGTTTGCACCAGAAGCTTTTGAGAAATTATATAATAAGGTAAAAGCATTTTTATCGAACAAAGAGGT
TTTTGTTCGTGATTCGTATGTTTGTTCAGATCCAAATTACAGATTAAACGTTCGCGTTGTTACCGAAACAGCTTGGTCGA
ACTTGTTTTGCTATAACATGTTCTTACGCCCGGAAGATTCTGAGCTGGCAAACTTTACTCCAGAATGGACAGTAGTATGC
GCTCCAAGTTTTATGGCAGATCCTGCTGTAGACGGAACACGTCAGTCAAATTTTGCGATTCTTGATTTTACTAAAAAAAT
TGCTTTAATTGGAGGAACAGGTTATACAGGAGAAATGAAAAAAGGAATTTTCTCTGCTTTAAACTTCATTTTACCAGTTT
TCGAAAACACATTACCAATGCACTGCAGCGCTAATGTTGGTAAAGATGGCGATACAGCAATTTTCTTTGGTTTATCGGGA
ACAGGAAAAACAACTTTATCTGCAGATCCGGAGCGTAAATTAATTGGAGACGATGAACACGGATGGACTGCTGAAAATAC
AGTTTTTAACTTTGAAGGAGGATGTTACGCTAAAGTAATCAACTTAACAGAAGAAAATGAACCAGACATTTTTAGAGCGA
TTAAAAAAGGAGCGCTTTTAGAAAATGTGGTCTTTAAAGCAGGAACTAATGAGGTTGATTTTGATGATGTTTCGATTACT
CAAAATACTCGTGTAAGTTACCCAATAACACATATCGACAATATTCAGCCGGGTTCTATTGGTCATAACCCCAAAAATAT
ATTTTTCCTTACAGCAGATTCTTTCGGAATTCTGCCTCCAATTTCAAAATTAACTCCGGGTCAGGCTGCTTACCATTTTA
TTTCTGGATATACAGCAAAAGTTGCCGGAACTGAAGCAGGAGTTACAGAACCACAGCCTAATTTCTCTGCTTGTTTTGGA
GCGCCATTTATGCCTTTGCATCCAACTAAATATGCTGAAATGTTAAGCAAAAAAATGAAAGATGCTGATGTAAAAGTTTG
GTTAATCAATACAGGATGGACTGGCGGTGCATACGGAACAGGAAGCCGTATGAAGTTAAAATATACTCGTGCTATGATTA
CTGCTGCATTAAACGGTGAACTAGATAATGTAGAGTATGTAAACCACAAAGTATTTGGAATTGCAAAACCACAATCTTGT
CCAAATGTTCCAAGCGAAATTTTAAATCCAAGAAACACTTGGGAAGACAAAGATTTATATGATAAAAAGGCGTTAGAATT
AGCACAGAAATTTAAAGCTAATTTTGCCAAATTTGAAGAGTTTGCCAATGCTGAAATCTTAGCAGGCGCACCGATTACAG
AATAA

Upstream 100 bases:

>100_bases
TAAAAAAATATAACAATTTGCAAACTTGGACTTTACGTTTAAGATTAAGTAAAATTATATTTTGTTGCACCCAAAAAATA
ACACACACAACATTAAATTT

Downstream 100 bases:

>100_bases
AAGGACTTACTAATTCAAAGTAAAAAAGCTGTTCAGAAATGAACAGCTTTTTTGTTTGGTTTTCAATCGCAGTAAAAAAA
AATAAACACTAACACGGGTA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MDNNTLFAQSISLKELGIENATVRYQLSADELHAITVQSGQGVEASTGALAINTGEFTGRSPQDRFIVKDSITEDKVWWG
NVNIPFAPEAFEKLYNKVKAFLSNKEVFVRDSYVCSDPNYRLNVRVVTETAWSNLFCYNMFLRPEDSELANFTPEWTVVC
APSFMADPAVDGTRQSNFAILDFTKKIALIGGTGYTGEMKKGIFSALNFILPVFENTLPMHCSANVGKDGDTAIFFGLSG
TGKTTLSADPERKLIGDDEHGWTAENTVFNFEGGCYAKVINLTEENEPDIFRAIKKGALLENVVFKAGTNEVDFDDVSIT
QNTRVSYPITHIDNIQPGSIGHNPKNIFFLTADSFGILPPISKLTPGQAAYHFISGYTAKVAGTEAGVTEPQPNFSACFG
APFMPLHPTKYAEMLSKKMKDADVKVWLINTGWTGGAYGTGSRMKLKYTRAMITAALNGELDNVEYVNHKVFGIAKPQSC
PNVPSEILNPRNTWEDKDLYDKKALELAQKFKANFAKFEEFANAEILAGAPITE

Sequences:

>Translated_534_residues
MDNNTLFAQSISLKELGIENATVRYQLSADELHAITVQSGQGVEASTGALAINTGEFTGRSPQDRFIVKDSITEDKVWWG
NVNIPFAPEAFEKLYNKVKAFLSNKEVFVRDSYVCSDPNYRLNVRVVTETAWSNLFCYNMFLRPEDSELANFTPEWTVVC
APSFMADPAVDGTRQSNFAILDFTKKIALIGGTGYTGEMKKGIFSALNFILPVFENTLPMHCSANVGKDGDTAIFFGLSG
TGKTTLSADPERKLIGDDEHGWTAENTVFNFEGGCYAKVINLTEENEPDIFRAIKKGALLENVVFKAGTNEVDFDDVSIT
QNTRVSYPITHIDNIQPGSIGHNPKNIFFLTADSFGILPPISKLTPGQAAYHFISGYTAKVAGTEAGVTEPQPNFSACFG
APFMPLHPTKYAEMLSKKMKDADVKVWLINTGWTGGAYGTGSRMKLKYTRAMITAALNGELDNVEYVNHKVFGIAKPQSC
PNVPSEILNPRNTWEDKDLYDKKALELAQKFKANFAKFEEFANAEILAGAPITE
>Mature_534_residues
MDNNTLFAQSISLKELGIENATVRYQLSADELHAITVQSGQGVEASTGALAINTGEFTGRSPQDRFIVKDSITEDKVWWG
NVNIPFAPEAFEKLYNKVKAFLSNKEVFVRDSYVCSDPNYRLNVRVVTETAWSNLFCYNMFLRPEDSELANFTPEWTVVC
APSFMADPAVDGTRQSNFAILDFTKKIALIGGTGYTGEMKKGIFSALNFILPVFENTLPMHCSANVGKDGDTAIFFGLSG
TGKTTLSADPERKLIGDDEHGWTAENTVFNFEGGCYAKVINLTEENEPDIFRAIKKGALLENVVFKAGTNEVDFDDVSIT
QNTRVSYPITHIDNIQPGSIGHNPKNIFFLTADSFGILPPISKLTPGQAAYHFISGYTAKVAGTEAGVTEPQPNFSACFG
APFMPLHPTKYAEMLSKKMKDADVKVWLINTGWTGGAYGTGSRMKLKYTRAMITAALNGELDNVEYVNHKVFGIAKPQSC
PNVPSEILNPRNTWEDKDLYDKKALELAQKFKANFAKFEEFANAEILAGAPITE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=515, Percent_Identity=50.0970873786408, Blast_Score=502, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6322950, Length=522, Percent_Identity=45.2107279693487, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_FLAJ1 (A5FK68)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193717.1
- ProteinModelPortal:   A5FK68
- SMR:   A5FK68
- STRING:   A5FK68
- GeneID:   5092371
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1366
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   PMHASAN
- ProtClustDB:   PRK09344
- BioCyc:   FJOH376686:FJOH_1366-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58802; Mature: 58802

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNNTLFAQSISLKELGIENATVRYQLSADELHAITVQSGQGVEASTGALAINTGEFTGR
CCCCEEEEEECCHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCC
SPQDRFIVKDSITEDKVWWGNVNIPFAPEAFEKLYNKVKAFLSNKEVFVRDSYVCSDPNY
CCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCE
RLNVRVVTETAWSNLFCYNMFLRPEDSELANFTPEWTVVCAPSFMADPAVDGTRQSNFAI
EEEEEEEEECCCCCEEEEEEEECCCCCHHHCCCCCEEEEECCCHHCCCCCCCCCCCCEEE
LDFTKKIALIGGTGYTGEMKKGIFSALNFILPVFENTLPMHCSANVGKDGDTAIFFGLSG
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEEECC
TGKTTLSADPERKLIGDDEHGWTAENTVFNFEGGCYAKVINLTEENEPDIFRAIKKGALL
CCCEEECCCCCCCCCCCCCCCCEECCEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCHH
ENVVFKAGTNEVDFDDVSITQNTRVSYPITHIDNIQPGSIGHNPKNIFFLTADSFGILPP
HHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
ISKLTPGQAAYHFISGYTAKVAGTEAGVTEPQPNFSACFGAPFMPLHPTKYAEMLSKKMK
HHHCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCC
DADVKVWLINTGWTGGAYGTGSRMKLKYTRAMITAALNGELDNVEYVNHKVFGIAKPQSC
CCCEEEEEEECCCCCCCCCCCCEEEEEEHHEEEEHHHCCCCCCEEEECEEEEEEECCCCC
PNVPSEILNPRNTWEDKDLYDKKALELAQKFKANFAKFEEFANAEILAGAPITE
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MDNNTLFAQSISLKELGIENATVRYQLSADELHAITVQSGQGVEASTGALAINTGEFTGR
CCCCEEEEEECCHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCC
SPQDRFIVKDSITEDKVWWGNVNIPFAPEAFEKLYNKVKAFLSNKEVFVRDSYVCSDPNY
CCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCE
RLNVRVVTETAWSNLFCYNMFLRPEDSELANFTPEWTVVCAPSFMADPAVDGTRQSNFAI
EEEEEEEEECCCCCEEEEEEEECCCCCHHHCCCCCEEEEECCCHHCCCCCCCCCCCCEEE
LDFTKKIALIGGTGYTGEMKKGIFSALNFILPVFENTLPMHCSANVGKDGDTAIFFGLSG
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEEECC
TGKTTLSADPERKLIGDDEHGWTAENTVFNFEGGCYAKVINLTEENEPDIFRAIKKGALL
CCCEEECCCCCCCCCCCCCCCCEECCEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCHH
ENVVFKAGTNEVDFDDVSITQNTRVSYPITHIDNIQPGSIGHNPKNIFFLTADSFGILPP
HHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
ISKLTPGQAAYHFISGYTAKVAGTEAGVTEPQPNFSACFGAPFMPLHPTKYAEMLSKKMK
HHHCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCC
DADVKVWLINTGWTGGAYGTGSRMKLKYTRAMITAALNGELDNVEYVNHKVFGIAKPQSC
CCCEEEEEEECCCCCCCCCCCCEEEEEEHHEEEEHHHCCCCCCEEEECEEEEEEECCCCC
PNVPSEILNPRNTWEDKDLYDKKALELAQKFKANFAKFEEFANAEILAGAPITE
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA