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The map label for this gene is prs [H]

Identifier: 146298887

GI number: 146298887

Start: 1274322

End: 1275263

Strand: Direct

Name: prs [H]

Synonym: Fjoh_1126

Alternate gene names: 146298887

Gene position: 1274322-1275263 (Clockwise)

Preceding gene: 146298885

Following gene: 146298888

Centisome position: 20.9

GC content: 37.05

Gene sequence:

>942_bases
ATGTCGCACCTAGAACCAGAAGCTAAAATTTTTGCTTGTTCACAAAGTGTTTATCTTGCAGAAAAAATTGCAGAACAGTA
CGGAATTCCGTTAGGAAAAGTAACGATGTCAACGTATAGTGATGGAGAATTTCAGCCGTCTTACGAAGAATCAATTAGAG
GTTTACGCGTTTTTATCGTGTGTTCAACTTTTCCATCGGCAGATAATTTGATGGAATTGTTATTAATGATTGATGCAGCA
AAACGTGCATCAGCAAGACATATTACAGCTGTTATGCCTTATTTTGGCTGGGCAAGACAGGATAGAAAAGATAAGCCAAG
AGTGCCGATTGGAGCTAAGTTAGTAGCAAACCTATTAACAGCTGCAGGAGCAACAAGAATCATGACGATGGATTTGCATG
CAGATCAAATTCAGGGATTCTTTGAAAAACCGGTAGATCATTTATTTGCATCTACAATCTTTTTGCCTTACGTGCAGAGT
TTAAATTTAGAAAATTTAACGATTGCATCTCCAGACATGGGAGGATCAAAAAGAGCTTATGCTTACTCTAAGTTTTTAGA
ATCAGATGTAGTAATCTGTTACAAACAAAGAAAAGCTGCCAATGTTATCGACACTATGGAATTGATTGGTGAAGTAAAAG
GTCGTAACGTAATCTTAGTAGATGACATGATCGATACAGGAGGAACTTTAGCGAAAGCTGCAGATCTTATGATCGAAAAA
GGAGCGCTAAGTGTAAGAGCAATTTGTACACATGCTATTTTATCTGGCGGTGCGTATGAAAAAATTGAAAATTCAAAATT
AACTGAGTTGATCGTAACCGATTCTATTCCGTTAAAGAAACATTCAGACAAGATTAGAGTAGTGAGTTGTGCACCTCTTT
TCGCAGAAGTTATGCACATGGTGCACCACAACAATTCCATTAGTGGAAAATTCATTATGTAA

Upstream 100 bases:

>100_bases
CACAACTAACTACATCCGAGACATTTATGCGTATTCTGCTTTATCTTAAAAGATATTGCTGGATTTTTATAAGCGTAACG
GAATAAAACAACAAATTATA

Downstream 100 bases:

>100_bases
AAGCTATAAGCTTAGGCAATAAGCCATAAGCTGGTAGAAATTACAAAAAAAGCCTAAAGCGTATGGCCTAAAGCTTACAA
CAATTAAGAATATTTATTTA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSHLEPEAKIFACSQSVYLAEKIAEQYGIPLGKVTMSTYSDGEFQPSYEESIRGLRVFIVCSTFPSADNLMELLLMIDAA
KRASARHITAVMPYFGWARQDRKDKPRVPIGAKLVANLLTAAGATRIMTMDLHADQIQGFFEKPVDHLFASTIFLPYVQS
LNLENLTIASPDMGGSKRAYAYSKFLESDVVICYKQRKAANVIDTMELIGEVKGRNVILVDDMIDTGGTLAKAADLMIEK
GALSVRAICTHAILSGGAYEKIENSKLTELIVTDSIPLKKHSDKIRVVSCAPLFAEVMHMVHHNNSISGKFIM

Sequences:

>Translated_313_residues
MSHLEPEAKIFACSQSVYLAEKIAEQYGIPLGKVTMSTYSDGEFQPSYEESIRGLRVFIVCSTFPSADNLMELLLMIDAA
KRASARHITAVMPYFGWARQDRKDKPRVPIGAKLVANLLTAAGATRIMTMDLHADQIQGFFEKPVDHLFASTIFLPYVQS
LNLENLTIASPDMGGSKRAYAYSKFLESDVVICYKQRKAANVIDTMELIGEVKGRNVILVDDMIDTGGTLAKAADLMIEK
GALSVRAICTHAILSGGAYEKIENSKLTELIVTDSIPLKKHSDKIRVVSCAPLFAEVMHMVHHNNSISGKFIM
>Mature_312_residues
SHLEPEAKIFACSQSVYLAEKIAEQYGIPLGKVTMSTYSDGEFQPSYEESIRGLRVFIVCSTFPSADNLMELLLMIDAAK
RASARHITAVMPYFGWARQDRKDKPRVPIGAKLVANLLTAAGATRIMTMDLHADQIQGFFEKPVDHLFASTIFLPYVQSL
NLENLTIASPDMGGSKRAYAYSKFLESDVVICYKQRKAANVIDTMELIGEVKGRNVILVDDMIDTGGTLAKAADLMIEKG
ALSVRAICTHAILSGGAYEKIENSKLTELIVTDSIPLKKHSDKIRVVSCAPLFAEVMHMVHHNNSISGKFIM

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=47.1153846153846, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI4506129, Length=312, Percent_Identity=44.5512820512821, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=45.5128205128205, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI84875539, Length=315, Percent_Identity=44.1269841269841, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI194018537, Length=337, Percent_Identity=34.4213649851632, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4506133, Length=335, Percent_Identity=31.6417910447761, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI310128524, Length=135, Percent_Identity=34.0740740740741, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI310115209, Length=135, Percent_Identity=34.0740740740741, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI310118259, Length=135, Percent_Identity=34.0740740740741, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI310119946, Length=135, Percent_Identity=34.0740740740741, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=46.7948717948718, Blast_Score=280, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=42.6282051282051, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=42.6282051282051, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=42.6282051282051, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=42.5324675324675, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17570245, Length=326, Percent_Identity=31.2883435582822, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319403, Length=308, Percent_Identity=43.8311688311688, Blast_Score=252, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6320946, Length=307, Percent_Identity=42.9967426710098, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321776, Length=299, Percent_Identity=43.8127090301003, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322667, Length=195, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324511, Length=114, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355239, Length=312, Percent_Identity=45.1923076923077, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=42.089552238806, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI281362873, Length=342, Percent_Identity=30.1169590643275, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24651454, Length=342, Percent_Identity=30.1169590643275, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24651458, Length=342, Percent_Identity=30.1169590643275, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24651456, Length=342, Percent_Identity=30.1169590643275, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45552010, Length=361, Percent_Identity=30.1939058171745, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24651462, Length=361, Percent_Identity=30.1939058171745, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24651464, Length=361, Percent_Identity=30.1939058171745, Blast_Score=144, Evalue=5e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34510; Mature: 34378

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLEPEAKIFACSQSVYLAEKIAEQYGIPLGKVTMSTYSDGEFQPSYEESIRGLRVFIV
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHCCCEEEEEE
CSTFPSADNLMELLLMIDAAKRASARHITAVMPYFGWARQDRKDKPRVPIGAKLVANLLT
EECCCCHHHHHHHHHHHHHHHHCCHHHHEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
AAGATRIMTMDLHADQIQGFFEKPVDHLFASTIFLPYVQSLNLENLTIASPDMGGSKRAY
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHH
AYSKFLESDVVICYKQRKAANVIDTMELIGEVKGRNVILVDDMIDTGGTLAKAADLMIEK
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GALSVRAICTHAILSGGAYEKIENSKLTELIVTDSIPLKKHSDKIRVVSCAPLFAEVMHM
CCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHH
VHHNNSISGKFIM
HHCCCCCCCCEEC
>Mature Secondary Structure 
SHLEPEAKIFACSQSVYLAEKIAEQYGIPLGKVTMSTYSDGEFQPSYEESIRGLRVFIV
CCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHCCCEEEEEE
CSTFPSADNLMELLLMIDAAKRASARHITAVMPYFGWARQDRKDKPRVPIGAKLVANLLT
EECCCCHHHHHHHHHHHHHHHHCCHHHHEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
AAGATRIMTMDLHADQIQGFFEKPVDHLFASTIFLPYVQSLNLENLTIASPDMGGSKRAY
HCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHH
AYSKFLESDVVICYKQRKAANVIDTMELIGEVKGRNVILVDDMIDTGGTLAKAADLMIEK
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GALSVRAICTHAILSGGAYEKIENSKLTELIVTDSIPLKKHSDKIRVVSCAPLFAEVMHM
CCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHH
VHHNNSISGKFIM
HHCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]