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The map label for this gene is purD [H]

Identifier: 146298802

GI number: 146298802

Start: 1185826

End: 1187100

Strand: Direct

Name: purD [H]

Synonym: Fjoh_1041

Alternate gene names: 146298802

Gene position: 1185826-1187100 (Clockwise)

Preceding gene: 146298798

Following gene: 146298804

Centisome position: 19.45

GC content: 35.53

Gene sequence:

>1275_bases
ATGACAATTTTACTATTGGGATCAGGCGGAAGAGAGCATGCATTTGCATGGAAAATGACTCAAAGTCCGCTTTGCGAAAA
ACTTTTTGTAGTACCGGGAAATGCAGGAACTGCTGCAATTGCTGAAAACGTTGCAATATCTCCAACTGATTTTGAAGCAG
TAAAAGCATTAGTGCTTAAAGAAAATATAAGTTTAGTAGTCGTAGGCCCAGAAGATCCGTTGGTAAAAGGAGTTTACGAT
TATTTTAAAAACGACGATAGTTTAAAACATATTCCGGTTATTGGGCCATCAAAACTTGGGGCGCAGTTAGAAGGAAGTAA
AGAATTTGCAAAAGAATTCTTGATGAAACATAATATCCCAACTGCAGCTTATGACAGTTTTACTGCCGAAACTGTTGAAG
AAGGATGTAAGTTTCTGGAAACTTTGCAGCCTCCTTACGTTTTAAAAGCCGACGGTTTAGCGGCTGGAAAAGGAGTTTTG
ATTATTCAGGATCTTGAAGAAGCAAAAACAGAATTACGCAACATGCTGGTTCACGCTAAATTTGGAACAGCAAGTTCAAA
AGTAGTTATTGAAGAATTCTTAGATGGAATCGAATTAAGCTGTTTCGTTTTAACAGATGGAAAAAGCTATAAAATTCTTC
CAACTGCAAAAGATTATAAAAGAATTGGTGAAGGAGACACAGGTTTAAATACAGGCGGAATGGGAGCAGTTTCTCCAGTT
CCTTATGTGGATGCTGTTTTGATGGAAAAAATTGAAACACGTATCGTAAAACCAACAATCGAAGGTTTTCAAAAAGATGG
AATCGAATACAAAGGATTTGTATTTATTGGTCTTATCAATGTAAAAGGAGAACCAATCGTTATTGAATACAATGTAAGAA
TGGGAGATCCTGAAACTGAAGTTGTGGTTCCAAGATTAAAATCTGACTTGGTTGAATTGTTTTTGTCTGTTGCAGATCAA
AAATTAGGAGACTTTAATTTAGAAGTTGATCCAAGAAGCGCAACAACTGTAATGGTAGTTTCTGGCGGATATCCTGAAGA
TTTTGAAAAAGGAAAAGTAATTTCTGGATTAGAAAATATTACAGATTCGATAGTTTTTCACGCAGGAACAAAATTAGATG
GCAAAAATGTCGTTACTAATGGAGGGCGTGTAATTGCTGTAACCTCTTATGGAGATAATTTCCAGGAGGCCATAAAAAAA
TCTTACCAAAACATAGATAAACTAAGCTTTGATAAGATGTATTTTAGAAAAGATATCGGCTTCGATCTAATTTAA

Upstream 100 bases:

>100_bases
GAATTACTAACAGTGCAGAACCAACAACTTTTTAAAGATTTAATTATTTTTTACGTACAAAAGCAAGTATTTTTGCACCA
CAACTAAATACAACAACACC

Downstream 100 bases:

>100_bases
AAAATATTTGAACCAGCCCTTTTAATTAAGGGTTTGGTTTTATTTTAAAAATGAATGAGCTGTTGTATCTTGGTTTTCTG
TTCCGGCATCATCAAAAATG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTILLLGSGGREHAFAWKMTQSPLCEKLFVVPGNAGTAAIAENVAISPTDFEAVKALVLKENISLVVVGPEDPLVKGVYD
YFKNDDSLKHIPVIGPSKLGAQLEGSKEFAKEFLMKHNIPTAAYDSFTAETVEEGCKFLETLQPPYVLKADGLAAGKGVL
IIQDLEEAKTELRNMLVHAKFGTASSKVVIEEFLDGIELSCFVLTDGKSYKILPTAKDYKRIGEGDTGLNTGGMGAVSPV
PYVDAVLMEKIETRIVKPTIEGFQKDGIEYKGFVFIGLINVKGEPIVIEYNVRMGDPETEVVVPRLKSDLVELFLSVADQ
KLGDFNLEVDPRSATTVMVVSGGYPEDFEKGKVISGLENITDSIVFHAGTKLDGKNVVTNGGRVIAVTSYGDNFQEAIKK
SYQNIDKLSFDKMYFRKDIGFDLI

Sequences:

>Translated_424_residues
MTILLLGSGGREHAFAWKMTQSPLCEKLFVVPGNAGTAAIAENVAISPTDFEAVKALVLKENISLVVVGPEDPLVKGVYD
YFKNDDSLKHIPVIGPSKLGAQLEGSKEFAKEFLMKHNIPTAAYDSFTAETVEEGCKFLETLQPPYVLKADGLAAGKGVL
IIQDLEEAKTELRNMLVHAKFGTASSKVVIEEFLDGIELSCFVLTDGKSYKILPTAKDYKRIGEGDTGLNTGGMGAVSPV
PYVDAVLMEKIETRIVKPTIEGFQKDGIEYKGFVFIGLINVKGEPIVIEYNVRMGDPETEVVVPRLKSDLVELFLSVADQ
KLGDFNLEVDPRSATTVMVVSGGYPEDFEKGKVISGLENITDSIVFHAGTKLDGKNVVTNGGRVIAVTSYGDNFQEAIKK
SYQNIDKLSFDKMYFRKDIGFDLI
>Mature_423_residues
TILLLGSGGREHAFAWKMTQSPLCEKLFVVPGNAGTAAIAENVAISPTDFEAVKALVLKENISLVVVGPEDPLVKGVYDY
FKNDDSLKHIPVIGPSKLGAQLEGSKEFAKEFLMKHNIPTAAYDSFTAETVEEGCKFLETLQPPYVLKADGLAAGKGVLI
IQDLEEAKTELRNMLVHAKFGTASSKVVIEEFLDGIELSCFVLTDGKSYKILPTAKDYKRIGEGDTGLNTGGMGAVSPVP
YVDAVLMEKIETRIVKPTIEGFQKDGIEYKGFVFIGLINVKGEPIVIEYNVRMGDPETEVVVPRLKSDLVELFLSVADQK
LGDFNLEVDPRSATTVMVVSGGYPEDFEKGKVISGLENITDSIVFHAGTKLDGKNVVTNGGRVIAVTSYGDNFQEAIKKS
YQNIDKLSFDKMYFRKDIGFDLI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=42.2897196261682, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=42.2897196261682, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=42.2897196261682, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=42.2897196261682, Blast_Score=336, Evalue=2e-92,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=48.1395348837209, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=40.0473933649289, Blast_Score=298, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=43.5955056179775, Blast_Score=342, Evalue=4e-95,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=39.3939393939394, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=39.3939393939394, Blast_Score=292, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46226; Mature: 46094

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILLLGSGGREHAFAWKMTQSPLCEKLFVVPGNAGTAAIAENVAISPTDFEAVKALVLK
CEEEEEECCCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHCCEECCCCCHHHHHHHHEE
ENISLVVVGPEDPLVKGVYDYFKNDDSLKHIPVIGPSKLGAQLEGSKEFAKEFLMKHNIP
CCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC
TAAYDSFTAETVEEGCKFLETLQPPYVLKADGLAAGKGVLIIQDLEEAKTELRNMLVHAK
CHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHEEEEE
FGTASSKVVIEEFLDGIELSCFVLTDGKSYKILPTAKDYKRIGEGDTGLNTGGMGAVSPV
CCCCCCHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCC
PYVDAVLMEKIETRIVKPTIEGFQKDGIEYKGFVFIGLINVKGEPIVIEYNVRMGDPETE
CHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEEECCCCCCE
VVVPRLKSDLVELFLSVADQKLGDFNLEVDPRSATTVMVVSGGYPEDFEKGKVISGLENI
EEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
TDSIVFHAGTKLDGKNVVTNGGRVIAVTSYGDNFQEAIKKSYQNIDKLSFDKMYFRKDIG
HHHEEEECCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC
FDLI
CCCC
>Mature Secondary Structure 
TILLLGSGGREHAFAWKMTQSPLCEKLFVVPGNAGTAAIAENVAISPTDFEAVKALVLK
EEEEEECCCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHCCEECCCCCHHHHHHHHEE
ENISLVVVGPEDPLVKGVYDYFKNDDSLKHIPVIGPSKLGAQLEGSKEFAKEFLMKHNIP
CCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC
TAAYDSFTAETVEEGCKFLETLQPPYVLKADGLAAGKGVLIIQDLEEAKTELRNMLVHAK
CHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHEEEEE
FGTASSKVVIEEFLDGIELSCFVLTDGKSYKILPTAKDYKRIGEGDTGLNTGGMGAVSPV
CCCCCCHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCC
PYVDAVLMEKIETRIVKPTIEGFQKDGIEYKGFVFIGLINVKGEPIVIEYNVRMGDPETE
CHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEEECCCCCCE
VVVPRLKSDLVELFLSVADQKLGDFNLEVDPRSATTVMVVSGGYPEDFEKGKVISGLENI
EEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
TDSIVFHAGTKLDGKNVVTNGGRVIAVTSYGDNFQEAIKKSYQNIDKLSFDKMYFRKDIG
HHHEEEECCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCC
FDLI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]