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The map label for this gene is lepA

Identifier: 146298548

GI number: 146298548

Start: 876411

End: 878207

Strand: Direct

Name: lepA

Synonym: Fjoh_0786

Alternate gene names: 146298548

Gene position: 876411-878207 (Clockwise)

Preceding gene: 146298546

Following gene: 146298549

Centisome position: 14.37

GC content: 36.45

Gene sequence:

>1797_bases
ATGAAGAAGATTCGTAACTTTTGCATTATTGCACACATTGACCACGGTAAAAGTACATTGGCGGACAGATTACTAGGCGC
AACACAAACCGTTACAGCTCGTGAAGAAAAAGCACAATTGCTTGACAACATGGACCTGGAGCGCGAACGTGGTATTACCA
TAAAAAGTCACGCCATTCAAATGGAATACAAATACAAAGGCGAAGAATATATCTTAAACTTAATTGATACCCCGGGACAC
GTTGACTTTTCATACGAAGTTTCAAGATCTATTGCTGCCTGCGAAGGTGCGCTTTTGATTGTAGATGCGGCTCAGAGTAT
TCAGGCACAAACGATTTCAAATTTATATTTGGCTCTTGAAAACGACTTAGAAATTATTCCGGTTTTAAACAAAGTTGATT
TACCAAGTGCTAATCCAGAAGAAGTAAGCGACGATATCATCGATTTATTAGGATGTAAATTAGAAGATATTATTCATGCT
TCGGGAAAAACTGGTTTTGGTGTTGAAAATATTTTGGCAGCAATTATTGAAAAAATCCCTCCTCCAAAAGGGGATCCGGA
AGAACCATTACAAGCTTTGATTTTTGACTCGGTTTACAATCCGTTCCGCGGTATTGAGGTAATCTTTAGAGTTGTAAATG
GTGAAATCAAAAAAGGCCAGAAAATTAAATTCATGGCTACTGATAACGAATATTTTGCTGACGAAATTGGAACTTTAAAA
TTAAATCAGGTTCCTAAAAATGTGGTTTCGGCAGGAGATGTTGGTTATTTGATTTCTGGAATTAAAGAAGCCCGCGAAGT
AAAAGTGGGTGATACAATTACAGATGCAAAAGTACCAACTACCAATATGATTACTGGTTTTGAGGATGTAAAACCAATGG
TATTTGCCGGAATTTATCCTGTTGACACAGAAGATTACGAAGATTTACGTTCTTCTATGGAAAAATTACAGCTTAATGAT
GCTTCATTAGTTTTTACTCCTGAAAGTTCTGCTGCATTAGGATTCGGTTTCCGCTGCGGATTCTTAGGAATGCTTCACAT
GGAAATTATCCAGGAACGTTTAGAGCGCGAATTCGATATGACTGTAATTACAACAGTTCCTAACGTTTCGTATTTGGCTT
ACACTAAAAAAGAACCTGAAAAAGCATTAATTGTAAATAATCCTTCAGATTTACCGGAACCTTCAAAACTAGACAGAGTT
GAAGAGCCTTTTATTAAAGCTACAATTATTACAAAAGCTGATTTCGTTGGAAACGTAATGAGTTTATGTATCGAAAAACG
TGGTTTAATTACCAACCAAACCTATTTAACAACAGAACGTGTTGAGTTAAACTTTGATATGCCTTTGGCCGAAATTGTAT
TCGATTTTTACGATCGTTTAAAAACAGTTTCTAAAGGTTATGCTTCTTTTGACTACTCTCCTATTGGAATGAGAACTTCA
AAATTAGTGAAACTTGACGTTCTTTTAAATGCACAAACGGTTGATGCACTTTCTGCATTGATCCATGAAGATAACGCGTA
CAACATCGGTAAAAAAATGACCGAGAAATTACGTGAGTTGATTCCGAGACAGCAATTCGATATTCCAATTCAGGCAGCAA
TTGGAGCAAAAATTATCGCTCGTGAAACGATTAAAGCGCTTCGTAAAGACGTTACCGCAAAATGTTACGGTGGAGATATT
TCACGTAAACGTAAACTTCTTGAAAAACAGAAAAAAGGTAAAAAACGTATGCGTCAGGTAGGAAACGTTGAAATTCCGCA
AGAAGCATTTATGGCTGTTTTGAAATTGAATGACTAA

Upstream 100 bases:

>100_bases
GCGCTATCTTTGCCACTTGATAATATGTGTTAAAGCGATTGCTACAGCGCATTTTACATCAAAAAAATACTTAAACTCGA
TAGAGAACTGATATACTAAA

Downstream 100 bases:

>100_bases
ATTTAGCATTAAGATTTAAGTACTAAATAGTAAGAATATATACAAACCCGATGACTAATGTTGTCGGGTTTTTGTTTGAA
AATACTTAATTATTATCCAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MKKIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNMDLERERGITIKSHAIQMEYKYKGEEYILNLIDTPGH
VDFSYEVSRSIAACEGALLIVDAAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHA
SGKTGFGVENILAAIIEKIPPPKGDPEEPLQALIFDSVYNPFRGIEVIFRVVNGEIKKGQKIKFMATDNEYFADEIGTLK
LNQVPKNVVSAGDVGYLISGIKEAREVKVGDTITDAKVPTTNMITGFEDVKPMVFAGIYPVDTEDYEDLRSSMEKLQLND
ASLVFTPESSAALGFGFRCGFLGMLHMEIIQERLEREFDMTVITTVPNVSYLAYTKKEPEKALIVNNPSDLPEPSKLDRV
EEPFIKATIITKADFVGNVMSLCIEKRGLITNQTYLTTERVELNFDMPLAEIVFDFYDRLKTVSKGYASFDYSPIGMRTS
KLVKLDVLLNAQTVDALSALIHEDNAYNIGKKMTEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDI
SRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLND

Sequences:

>Translated_598_residues
MKKIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNMDLERERGITIKSHAIQMEYKYKGEEYILNLIDTPGH
VDFSYEVSRSIAACEGALLIVDAAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHA
SGKTGFGVENILAAIIEKIPPPKGDPEEPLQALIFDSVYNPFRGIEVIFRVVNGEIKKGQKIKFMATDNEYFADEIGTLK
LNQVPKNVVSAGDVGYLISGIKEAREVKVGDTITDAKVPTTNMITGFEDVKPMVFAGIYPVDTEDYEDLRSSMEKLQLND
ASLVFTPESSAALGFGFRCGFLGMLHMEIIQERLEREFDMTVITTVPNVSYLAYTKKEPEKALIVNNPSDLPEPSKLDRV
EEPFIKATIITKADFVGNVMSLCIEKRGLITNQTYLTTERVELNFDMPLAEIVFDFYDRLKTVSKGYASFDYSPIGMRTS
KLVKLDVLLNAQTVDALSALIHEDNAYNIGKKMTEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDI
SRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLND
>Mature_598_residues
MKKIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNMDLERERGITIKSHAIQMEYKYKGEEYILNLIDTPGH
VDFSYEVSRSIAACEGALLIVDAAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHA
SGKTGFGVENILAAIIEKIPPPKGDPEEPLQALIFDSVYNPFRGIEVIFRVVNGEIKKGQKIKFMATDNEYFADEIGTLK
LNQVPKNVVSAGDVGYLISGIKEAREVKVGDTITDAKVPTTNMITGFEDVKPMVFAGIYPVDTEDYEDLRSSMEKLQLND
ASLVFTPESSAALGFGFRCGFLGMLHMEIIQERLEREFDMTVITTVPNVSYLAYTKKEPEKALIVNNPSDLPEPSKLDRV
EEPFIKATIITKADFVGNVMSLCIEKRGLITNQTYLTTERVELNFDMPLAEIVFDFYDRLKTVSKGYASFDYSPIGMRTS
KLVKLDVLLNAQTVDALSALIHEDNAYNIGKKMTEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDI
SRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLND

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=49.4176372712146, Blast_Score=618, Evalue=1e-177,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=44.2748091603053, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI310132016, Length=108, Percent_Identity=43.5185185185185, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310110807, Length=108, Percent_Identity=43.5185185185185, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI310123363, Length=108, Percent_Identity=43.5185185185185, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306283, Length=136, Percent_Identity=41.9117647058824, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306287, Length=141, Percent_Identity=41.1347517730496, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI19923640, Length=141, Percent_Identity=41.1347517730496, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=38.4615384615385, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI18390331, Length=144, Percent_Identity=38.1944444444444, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=33.0827067669173, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=33.0827067669173, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI34147630, Length=277, Percent_Identity=28.5198555956679, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI94966752, Length=93, Percent_Identity=36.5591397849462, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=54.2713567839196, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=511, Percent_Identity=28.5714285714286, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI1789738, Length=158, Percent_Identity=35.4430379746835, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=32.7044025157233, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=41.5711947626841, Blast_Score=483, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17556745, Length=204, Percent_Identity=33.8235294117647, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=39.0977443609023, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=39.0977443609023, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=157, Percent_Identity=36.9426751592357, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=34.3949044585987, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.0827067669173, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=142, Percent_Identity=35.2112676056338, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=43.8741721854305, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=36.0655737704918, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=40.5594405594406, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=40.5594405594406, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=41.1347517730496, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=39.4736842105263, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=284, Percent_Identity=27.4647887323944, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=44.2595673876872, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=44.2028985507246, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=37.1621621621622, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=37.0860927152318, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=34.5864661654135, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363316, Length=283, Percent_Identity=28.6219081272085, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17864358, Length=283, Percent_Identity=28.6219081272085, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28572034, Length=222, Percent_Identity=30.6306306306306, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921738, Length=287, Percent_Identity=26.1324041811847, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550900, Length=325, Percent_Identity=26.4615384615385, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_FLAJ1 (A5FLU8)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193139.1
- ProteinModelPortal:   A5FLU8
- SMR:   A5FLU8
- STRING:   A5FLU8
- GeneID:   5093724
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_0786
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   FJOH376686:FJOH_0786-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66826; Mature: 66826

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNMDLERERGITIKSHAIQ
CCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCEEEEEEEE
MEYKYKGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQSIQAQTISNLYLALE
EEEEECCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHEEEEC
NDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHASGKTGFGVENILAAIIEKIP
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHCC
PPKGDPEEPLQALIFDSVYNPFRGIEVIFRVVNGEIKKGQKIKFMATDNEYFADEIGTLK
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCEE
LNQVPKNVVSAGDVGYLISGIKEAREVKVGDTITDAKVPTTNMITGFEDVKPMVFAGIYP
ECCCCHHHHCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHCCCHHHCCCCHHCCCCC
VDTEDYEDLRSSMEKLQLNDASLVFTPESSAALGFGFRCGFLGMLHMEIIQERLEREFDM
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
TVITTVPNVSYLAYTKKEPEKALIVNNPSDLPEPSKLDRVEEPFIKATIITKADFVGNVM
EEEEECCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHH
SLCIEKRGLITNQTYLTTERVELNFDMPLAEIVFDFYDRLKTVSKGYASFDYSPIGMRTS
HHHHHHCCCEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KLVKLDVLLNAQTVDALSALIHEDNAYNIGKKMTEKLRELIPRQQFDIPIQAAIGAKIIA
CEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHH
RETIKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLND
HHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKKIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNMDLERERGITIKSHAIQ
CCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCEEEEEEEE
MEYKYKGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQSIQAQTISNLYLALE
EEEEECCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHEEEEC
NDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHASGKTGFGVENILAAIIEKIP
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHCC
PPKGDPEEPLQALIFDSVYNPFRGIEVIFRVVNGEIKKGQKIKFMATDNEYFADEIGTLK
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCEE
LNQVPKNVVSAGDVGYLISGIKEAREVKVGDTITDAKVPTTNMITGFEDVKPMVFAGIYP
ECCCCHHHHCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHCCCHHHCCCCHHCCCCC
VDTEDYEDLRSSMEKLQLNDASLVFTPESSAALGFGFRCGFLGMLHMEIIQERLEREFDM
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
TVITTVPNVSYLAYTKKEPEKALIVNNPSDLPEPSKLDRVEEPFIKATIITKADFVGNVM
EEEEECCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHH
SLCIEKRGLITNQTYLTTERVELNFDMPLAEIVFDFYDRLKTVSKGYASFDYSPIGMRTS
HHHHHHCCCEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KLVKLDVLLNAQTVDALSALIHEDNAYNIGKKMTEKLRELIPRQQFDIPIQAAIGAKIIA
CEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHH
RETIKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLND
HHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA