Click here to switch to the map view.

The map label for this gene is bsp6IM [H]

Identifier: 146298415

GI number: 146298415

Start: 711290

End: 712279

Strand: Direct

Name: bsp6IM [H]

Synonym: Fjoh_0652

Alternate gene names: 146298415

Gene position: 711290-712279 (Clockwise)

Preceding gene: 146298413

Following gene: 146298420

Centisome position: 11.67

GC content: 34.04

Gene sequence:

>990_bases
ATGAAGAAAACAGTTGGAAGTTTGTATGCTGGTGTAGGCGGTATATGTTTGGGTTTTAAAAAAGCGGGGTTTGAACTAGA
ATGGGCAAATGAATTTGACAAAAATGCCTGTATAACCTACAAAAATAATTTTGAACATAATCTTATTGAAGGTGATGTAA
TGGCTCTGGACGTTACTTCGCTTAAAAAAATAAACATTTTAACTGCAGGATTTCCCTGTCAGCCATTTTCGGTTGCGGGC
TATCGAAAAGGTTTTGAGGACAGCAGAGGAAATCATTTCTTTAAAATTTTGGATTTTGTAGACGAAATGCGCCCTAATGT
TATTTTTCTTGAAAATGTAAAAAACCTAAAAGGGCACGACAAAGGAAACACTATCAAAGTTATCGAAAACGAAATCAGAA
AACGCGGTTATACTTTTGACAGCGCAGTTTTGAACACCAAAGATTTTGGAAACATTCCTCATAACAGAGAAAGAATTTTC
ATTGTTGCTTTTGACAAAGATTATATCAAAGATGATAAATTCGAGTTTCATTTCCCTGAAAAAGAAGACTTAACCAAATC
GATCAAGGATTTAATTACGAAAGAAAAAGTCGACGAGAAATTTTATTATCACGAAGATAAATATATGTATGATATGCTGA
GAGAATCTATTACAAACAAGGATAGAATTTATCAGTTTAGACGACAATATGTTCGTGAAAACAAATCGAGCGTCTGCCCT
ACCCTTACTGCCAATATGGGAACCGGCGGCCACAATGTACCGCTTATTACAACCGATTTTGGTTTTAGAAAACTAACCCC
AAGAGAATGTTTTAGATTTCAGGGTTTTCCTGATGATTTTAAACTTCCTGCTATTTCAAATTCGCATTTGTATAAACAGG
CTGGAAACTCTGTAAGTATGCCTGTAATTGAAAGACTGGCATCTGAAATTTATAAAGTACTTGAAAAAGTAGAATCGAAA
AAGACAAAAAAATCTAAAGAAAAAGCTTAA

Upstream 100 bases:

>100_bases
TTTAAATTCTCACATTGATCAAGTGTTTCCGAATTAATATATTTGCATAATTATAAGAGAACCTAAAAAGGTCTTTTAAT
TAAATCAGGACAAATAGTAA

Downstream 100 bases:

>100_bases
ACTGTCCATCCTGAGCTTCTGATATTTTTTCTACAAAAGATTCGTATTCGTCTAATTTGGTAAAATCTATAAAATAGTCT
TGCTCTGTCGATTTTGTAAT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKTVGSLYAGVGGICLGFKKAGFELEWANEFDKNACITYKNNFEHNLIEGDVMALDVTSLKKINILTAGFPCQPFSVAG
YRKGFEDSRGNHFFKILDFVDEMRPNVIFLENVKNLKGHDKGNTIKVIENEIRKRGYTFDSAVLNTKDFGNIPHNRERIF
IVAFDKDYIKDDKFEFHFPEKEDLTKSIKDLITKEKVDEKFYYHEDKYMYDMLRESITNKDRIYQFRRQYVRENKSSVCP
TLTANMGTGGHNVPLITTDFGFRKLTPRECFRFQGFPDDFKLPAISNSHLYKQAGNSVSMPVIERLASEIYKVLEKVESK
KTKKSKEKA

Sequences:

>Translated_329_residues
MKKTVGSLYAGVGGICLGFKKAGFELEWANEFDKNACITYKNNFEHNLIEGDVMALDVTSLKKINILTAGFPCQPFSVAG
YRKGFEDSRGNHFFKILDFVDEMRPNVIFLENVKNLKGHDKGNTIKVIENEIRKRGYTFDSAVLNTKDFGNIPHNRERIF
IVAFDKDYIKDDKFEFHFPEKEDLTKSIKDLITKEKVDEKFYYHEDKYMYDMLRESITNKDRIYQFRRQYVRENKSSVCP
TLTANMGTGGHNVPLITTDFGFRKLTPRECFRFQGFPDDFKLPAISNSHLYKQAGNSVSMPVIERLASEIYKVLEKVESK
KTKKSKEKA
>Mature_329_residues
MKKTVGSLYAGVGGICLGFKKAGFELEWANEFDKNACITYKNNFEHNLIEGDVMALDVTSLKKINILTAGFPCQPFSVAG
YRKGFEDSRGNHFFKILDFVDEMRPNVIFLENVKNLKGHDKGNTIKVIENEIRKRGYTFDSAVLNTKDFGNIPHNRERIF
IVAFDKDYIKDDKFEFHFPEKEDLTKSIKDLITKEKVDEKFYYHEDKYMYDMLRESITNKDRIYQFRRQYVRENKSSVCP
TLTANMGTGGHNVPLITTDFGFRKLTPRECFRFQGFPDDFKLPAISNSHLYKQAGNSVSMPVIERLASEIYKVLEKVESK
KTKKSKEKA

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=166, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788271, Length=357, Percent_Identity=30.2521008403361, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 38101; Mature: 38101

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVGSLYAGVGGICLGFKKAGFELEWANEFDKNACITYKNNFEHNLIEGDVMALDVTS
CCCCHHHHHHCHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEEEEECCC
LKKINILTAGFPCQPFSVAGYRKGFEDSRGNHFFKILDFVDEMRPNVIFLENVKNLKGHD
CCEEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCC
KGNTIKVIENEIRKRGYTFDSAVLNTKDFGNIPHNRERIFIVAFDKDYIKDDKFEFHFPE
CCCEEHHHHHHHHHCCCCCHHHEECCCCCCCCCCCCCEEEEEEECCHHCCCCCEEEECCC
KEDLTKSIKDLITKEKVDEKFYYHEDKYMYDMLRESITNKDRIYQFRRQYVRENKSSVCP
HHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
TLTANMGTGGHNVPLITTDFGFRKLTPRECFRFQGFPDDFKLPAISNSHLYKQAGNSVSM
EEEECCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCH
PVIERLASEIYKVLEKVESKKTKKSKEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKTVGSLYAGVGGICLGFKKAGFELEWANEFDKNACITYKNNFEHNLIEGDVMALDVTS
CCCCHHHHHHCHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEEEEECCC
LKKINILTAGFPCQPFSVAGYRKGFEDSRGNHFFKILDFVDEMRPNVIFLENVKNLKGHD
CCEEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCC
KGNTIKVIENEIRKRGYTFDSAVLNTKDFGNIPHNRERIFIVAFDKDYIKDDKFEFHFPE
CCCEEHHHHHHHHHCCCCCHHHEECCCCCCCCCCCCCEEEEEEECCHHCCCCCEEEECCC
KEDLTKSIKDLITKEKVDEKFYYHEDKYMYDMLRESITNKDRIYQFRRQYVRENKSSVCP
HHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
TLTANMGTGGHNVPLITTDFGFRKLTPRECFRFQGFPDDFKLPAISNSHLYKQAGNSVSM
EEEECCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCH
PVIERLASEIYKVLEKVESKKTKKSKEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]