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The map label for this gene is argF [H]

Identifier: 229577459

GI number: 229577459

Start: 3232768

End: 3233688

Strand: Direct

Name: argF [H]

Synonym: PST_3003

Alternate gene names: 229577459

Gene position: 3232768-3233688 (Clockwise)

Preceding gene: 146283331

Following gene: 146283334

Centisome position: 70.78

GC content: 64.82

Gene sequence:

>921_bases
ATGAGCGCAAGGCATTTTCTCTCACTGATGGACTGCACGCCCCAGGAGCTGAACAGCCTGGTCCGCCGCGGCATCGAGTT
GAAGGATCTGCGCAAGCGCGGCGTCCTGTTCGAGCCCTTGAAGAATCGCGTGCTGGGCATGATCTTCGAGAAGGCCTCGA
CCCGCACCCGACTGTCCTTCGAAGCCGGCATGATCCAGCTCGGCGGCCAGGCGATCTTCCTTTCCCCGCGCGACACCCAG
CTTGGCCGCGGCGAGCCGATCAGCGACTCGGCCATCGTCATGTCGCGCATGCTCGACGCGGTGATGATCCGTACTTTCGC
CCATTCGACCCTGACCGACTTCGCCGCCAACTCCAGCGTACCGGTGATCAACGGCCTGTCCGATGACCTGCACCCCTGCC
AGCTGATGGCCGACATGCAGACCTACCACGAACACCGCGGCAGCATTGCCGGCAAGACGGTGGCCTGGATCGGCGACGGT
AACAACATGTGCAACACCTATATAGAAGCGGCCATCCAGTTCGACTTCCGGCTGAAGGTCGCCTGCCCCGAAGGCTACGA
ACCCGACGCCGAGCTGCTGGCACGCGCCGGCGAGCGTGTGCAGGTGCTGCGCGACCCACGCGAGGCAGCGGCGGGCGCCA
ACCTGATCAGCACCGACGTCTGGGCATCCATGGGCCAGGAAGATGAAGTGGCCGCGCGCCTGGCCACCTTCCGCCCCTAT
CAGGTGAACCGCGCGCTGCTCGACTGCGCCGCCGCGGACGTGCTGTTCATGCACTGCCTGCCGGCCCACCGTGGCGAGGA
AATCAGTCACGATCTGCTCGATGATCCCCGCTCGGCCGCTTGGGATCAGGCCGAGAACCGCCTGCACGTGCAGAAGGCAC
TGCTGGAGTTTCTTGTCGAGCCGGCCTATCACCACGCATGA

Upstream 100 bases:

>100_bases
CTTTCCCGTTTCGTCGCTCCCCGTGCGACTCCCCAGTCGTCGGTCCCCTTTTCTGTCGAAAATCCTCGGATCGCGCGCTG
CGGCAATAAAGGTAGTTGAT

Downstream 100 bases:

>100_bases
AACCTGAACTGCCGCTGCTACAACTGCGCGACCTGGCCTGCGGCTATGGTGAGCAGAGCATCGTTCAGCATCTCAACCTG
CACCTGAATCGCGGCGACAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSARHFLSLMDCTPQELNSLVRRGIELKDLRKRGVLFEPLKNRVLGMIFEKASTRTRLSFEAGMIQLGGQAIFLSPRDTQ
LGRGEPISDSAIVMSRMLDAVMIRTFAHSTLTDFAANSSVPVINGLSDDLHPCQLMADMQTYHEHRGSIAGKTVAWIGDG
NNMCNTYIEAAIQFDFRLKVACPEGYEPDAELLARAGERVQVLRDPREAAAGANLISTDVWASMGQEDEVAARLATFRPY
QVNRALLDCAAADVLFMHCLPAHRGEEISHDLLDDPRSAAWDQAENRLHVQKALLEFLVEPAYHHA

Sequences:

>Translated_306_residues
MSARHFLSLMDCTPQELNSLVRRGIELKDLRKRGVLFEPLKNRVLGMIFEKASTRTRLSFEAGMIQLGGQAIFLSPRDTQ
LGRGEPISDSAIVMSRMLDAVMIRTFAHSTLTDFAANSSVPVINGLSDDLHPCQLMADMQTYHEHRGSIAGKTVAWIGDG
NNMCNTYIEAAIQFDFRLKVACPEGYEPDAELLARAGERVQVLRDPREAAAGANLISTDVWASMGQEDEVAARLATFRPY
QVNRALLDCAAADVLFMHCLPAHRGEEISHDLLDDPRSAAWDQAENRLHVQKALLEFLVEPAYHHA
>Mature_305_residues
SARHFLSLMDCTPQELNSLVRRGIELKDLRKRGVLFEPLKNRVLGMIFEKASTRTRLSFEAGMIQLGGQAIFLSPRDTQL
GRGEPISDSAIVMSRMLDAVMIRTFAHSTLTDFAANSSVPVINGLSDDLHPCQLMADMQTYHEHRGSIAGKTVAWIGDGN
NMCNTYIEAAIQFDFRLKVACPEGYEPDAELLARAGERVQVLRDPREAAAGANLISTDVWASMGQEDEVAARLATFRPYQ
VNRALLDCAAADVLFMHCLPAHRGEEISHDLLDDPRSAAWDQAENRLHVQKALLEFLVEPAYHHA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=295, Percent_Identity=42.0338983050847, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=27.5409836065574, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1790703, Length=325, Percent_Identity=36.3076923076923, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786469, Length=325, Percent_Identity=35.0769230769231, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=29.6774193548387, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994908, Length=352, Percent_Identity=23.5795454545455, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=307, Percent_Identity=27.3615635179153, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=320, Percent_Identity=32.8125, Blast_Score=187, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=28.4313725490196, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=25.3205128205128, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34001; Mature: 33870

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARHFLSLMDCTPQELNSLVRRGIELKDLRKRGVLFEPLKNRVLGMIFEKASTRTRLSF
CCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEH
EAGMIQLGGQAIFLSPRDTQLGRGEPISDSAIVMSRMLDAVMIRTFAHSTLTDFAANSSV
HCCCEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVINGLSDDLHPCQLMADMQTYHEHRGSIAGKTVAWIGDGNNMCNTYIEAAIQFDFRLKV
CEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEEEE
ACPEGYEPDAELLARAGERVQVLRDPREAAAGANLISTDVWASMGQEDEVAARLATFRPY
ECCCCCCCCHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCH
QVNRALLDCAAADVLFMHCLPAHRGEEISHDLLDDPRSAAWDQAENRLHVQKALLEFLVE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
PAYHHA
HHHCCC
>Mature Secondary Structure 
SARHFLSLMDCTPQELNSLVRRGIELKDLRKRGVLFEPLKNRVLGMIFEKASTRTRLSF
CHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEH
EAGMIQLGGQAIFLSPRDTQLGRGEPISDSAIVMSRMLDAVMIRTFAHSTLTDFAANSSV
HCCCEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVINGLSDDLHPCQLMADMQTYHEHRGSIAGKTVAWIGDGNNMCNTYIEAAIQFDFRLKV
CEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEEEEEEE
ACPEGYEPDAELLARAGERVQVLRDPREAAAGANLISTDVWASMGQEDEVAARLATFRPY
ECCCCCCCCHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCH
QVNRALLDCAAADVLFMHCLPAHRGEEISHDLLDDPRSAAWDQAENRLHVQKALLEFLVE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
PAYHHA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3131308; 10984043 [H]