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The map label for this gene is cynT [H]

Identifier: 146284471

GI number: 146284471

Start: 4514696

End: 4515448

Strand: Direct

Name: cynT [H]

Synonym: PST_4160

Alternate gene names: 146284471

Gene position: 4514696-4515448 (Clockwise)

Preceding gene: 146284470

Following gene: 146284472

Centisome position: 98.85

GC content: 68.26

Gene sequence:

>753_bases
ATGCGTGAAATATTCACCGCCCGCGTCGAGCCCAGCAAAGCATCCGCCCAAGGCGGCCGGCATGCCCGAGACGAGAGTGC
CGACGAATCCTTCGCCCACCTGGTCGATGGCTTTCGCCGCTTCCGTGCCGAGGTCTACCCCGAGCAGCAGGCGCTGTTCG
CCCGTCTGGCCAAGGCCCAGCAGCCGCGCGCGATGTTCATCACCTGCGCGGACTCGCGGATCGTTCCCGAGCTGATCACC
CAGAGTTCGCCCGGCGACCTGTTCGTCACCCGCAACGTGGGCAACGTGGTGCCACCCTATGGCCAGATGAACGGCGGGGT
TTCCAGCGCCATCGAGTACGCGGTGATGGCCCTCGACGTGCAACACATCATTGTCTGCGGGCATTCCGACTGCGGTGCGA
TGAAAGCGGTGCTCGACCCGGCCGGGCTGCAGCGCATGCCCACGGTGAAGGCCTGGCTGCGCCATTGCGAGGTGGCGCGC
AGCCTGGTCGAGCAGAACTGCAGCTGCACGGCCGGCGAGGCCCTGGGCGTGCTTACCGAGGAGAACGTGGTGGCGCAGCT
CGACCACCTGCGCACCCACCCTTCGGTCGCCGCCCGCCTCGCCGGCGGTCAGCTGTCGATCCATGGCTGGGTCTACTGCA
TCGAAACCAGCGAAATCCTCGCCTACGACGCCGGCACCGGCCGCTTCGCGCCGCTGGACGGCGACGGCCCGCTGCCGGTG
GCAACGCCCGCACCGCGCTACCTGCAAGCCTGA

Upstream 100 bases:

>100_bases
TGACTAGCCTCGAGGCAAATGTGACCAGCCAGTCACGCGGACCGCCTGCGGTCTGCGCGGCGCTGGCACGCCTTGCCCCT
GCCCCAGGAGACCCAAGCCC

Downstream 100 bases:

>100_bases
ACCAGCGGCCGCAAAGGCATTGGCTGTCACTCCCGGCGGCGCCGCTGGGCGTCGCCGCGTTGTCGAGGATTTCGGATGAA
TGCAGAAACGATCAAAGCAA

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MREIFTARVEPSKASAQGGRHARDESADESFAHLVDGFRRFRAEVYPEQQALFARLAKAQQPRAMFITCADSRIVPELIT
QSSPGDLFVTRNVGNVVPPYGQMNGGVSSAIEYAVMALDVQHIIVCGHSDCGAMKAVLDPAGLQRMPTVKAWLRHCEVAR
SLVEQNCSCTAGEALGVLTEENVVAQLDHLRTHPSVAARLAGGQLSIHGWVYCIETSEILAYDAGTGRFAPLDGDGPLPV
ATPAPRYLQA

Sequences:

>Translated_250_residues
MREIFTARVEPSKASAQGGRHARDESADESFAHLVDGFRRFRAEVYPEQQALFARLAKAQQPRAMFITCADSRIVPELIT
QSSPGDLFVTRNVGNVVPPYGQMNGGVSSAIEYAVMALDVQHIIVCGHSDCGAMKAVLDPAGLQRMPTVKAWLRHCEVAR
SLVEQNCSCTAGEALGVLTEENVVAQLDHLRTHPSVAARLAGGQLSIHGWVYCIETSEILAYDAGTGRFAPLDGDGPLPV
ATPAPRYLQA
>Mature_250_residues
MREIFTARVEPSKASAQGGRHARDESADESFAHLVDGFRRFRAEVYPEQQALFARLAKAQQPRAMFITCADSRIVPELIT
QSSPGDLFVTRNVGNVVPPYGQMNGGVSSAIEYAVMALDVQHIIVCGHSDCGAMKAVLDPAGLQRMPTVKAWLRHCEVAR
SLVEQNCSCTAGEALGVLTEENVVAQLDHLRTHPSVAARLAGGQLSIHGWVYCIETSEILAYDAGTGRFAPLDGDGPLPV
ATPAPRYLQA

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=202, Percent_Identity=48.5148514851485, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1786318, Length=174, Percent_Identity=36.7816091954023, Blast_Score=97, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI115532990, Length=237, Percent_Identity=28.6919831223629, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25147564, Length=236, Percent_Identity=27.1186440677966, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324292, Length=171, Percent_Identity=28.6549707602339, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24645213, Length=240, Percent_Identity=27.5, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 26969; Mature: 26969

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIFTARVEPSKASAQGGRHARDESADESFAHLVDGFRRFRAEVYPEQQALFARLAKAQ
CCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
QPRAMFITCADSRIVPELITQSSPGDLFVTRNVGNVVPPYGQMNGGVSSAIEYAVMALDV
CCCEEEEEECCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCE
QHIIVCGHSDCGAMKAVLDPAGLQRMPTVKAWLRHCEVARSLVEQNCSCTAGEALGVLTE
EEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
ENVVAQLDHLRTHPSVAARLAGGQLSIHGWVYCIETSEILAYDAGTGRFAPLDGDGPLPV
CHHHHHHHHHHCCHHHHHHHCCCEEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCC
ATPAPRYLQA
CCCCCHHHCC
>Mature Secondary Structure
MREIFTARVEPSKASAQGGRHARDESADESFAHLVDGFRRFRAEVYPEQQALFARLAKAQ
CCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
QPRAMFITCADSRIVPELITQSSPGDLFVTRNVGNVVPPYGQMNGGVSSAIEYAVMALDV
CCCEEEEEECCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCE
QHIIVCGHSDCGAMKAVLDPAGLQRMPTVKAWLRHCEVARSLVEQNCSCTAGEALGVLTE
EEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
ENVVAQLDHLRTHPSVAARLAGGQLSIHGWVYCIETSEILAYDAGTGRFAPLDGDGPLPV
CHHHHHHHHHHCCHHHHHHHCCCEEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCC
ATPAPRYLQA
CCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]