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The map label for this gene is betT [H]

Identifier: 146284413

GI number: 146284413

Start: 4455375

End: 4457429

Strand: Direct

Name: betT [H]

Synonym: PST_4102

Alternate gene names: 146284413

Gene position: 4455375-4457429 (Clockwise)

Preceding gene: 146284412

Following gene: 146284414

Centisome position: 97.55

GC content: 62.73

Gene sequence:

>2055_bases
ATGGACGCCGCTAGGAAATTGCCCAAGGCCATCATTCTGTTTCCCGTGTTCATTCCTGCGGTGGTGATCACGCTGCTGCT
GGTGATCGGCACGGCGAGCAATCCGGAACTGGCCGGCGAGCTGTTCGATTCGGTGCTGGCATACATCACCCGCACCTTCG
GCTGGTTCTACATGCTGTCGGTGGCGTTCTTCCTGGTGTTCATCGTCGGCATCGCCCTGACCAAATGGGGCAACATCAAG
CTCGGGCCGGACCACTCCGAGCCGCAGTACAGTTTCCCTGCCTGGTTCGCCATGCTGTTCTCCGCCGGCTACGGCATCGC
CCTGCTGTTCTTCGGTGTCGCCGAGCCGGTGCTGCATTATTCGACGCCACCGGCCGGCGCGCCGGAGACGGTGGATGCCG
CCAAGCAAGCCATGCAGATCGCCTTCTTCCACTGGGGCTTTCACATCTGGGGCATCTTCGGCATCGTCGGCCTGGCGCTG
GCCTATTTCGCCTTCCGCCATGGTCTGCCGCTGTCGCTGCGCTCGGCGCTGTATCCGCTGATCGGCGAACGCATTTACGG
GCCGATCGGCCATTTCGTCGACGTGCTGGTAATCCTCGGCACGCTGTTCGGCATCGCCACCACGCTCGGCCTGTCGGTCA
CCCAGATCAACGCCGGCCTGAACTATCTGTGGGACGTACCAGTGGACATCACGGTGCAGGTGATCACCATCGCCATCATC
ACCTGCATGGCCATCGCCTCGGTGGTCGCCGGGCTCGACAAGGGGGTGAAGAACCTGTCGCTGCTCAACATCGTGCTGGC
CACGGTGCTGATGCTGTTCGTGCTCGCAGTCGGCCCGACCATCTTCATCCTGGAGACCTTTCTGCAGAACACCGGCAGCT
ACCTGAACAACATCGTCGAGCGCACCTTCAACCTGCAGGCCTACAGCCGCAGCGACTGGATCGGCAACTGGACGCTGTTC
ATCTTCGGCTGGACCATCTCCTGGTCGCCCTTCGTCGGCTTGTTCATCGCCAAGATCAGCCGTGGTCGGACCATCCGCCA
GTTTGTCTTCGGCGTGATGATCGTGCCGACCATGTTCACCTTCTTCTGGTTCTCGGTGTTCGGCGACACGGCCCTGCACC
TGATCATGGTCGAGGGGTACAACACGCTGATCCAGGATGTGCAGGCCGACCACGCGGTGGCGCTGTTCAAGCTGTTCGAG
CTATTGCCCTGGACCACACTGACCTCGATCCTCGCGGTGCTGCTGATCGTGACCTTCTTCGTCACCTCCTCGGACTCCGG
CTCGCTGGTAATCGACTCCATCGCCGCCGGCGGCGCCATCCATACGCCGACCTGGCAGCGCGTGTTCTGGGCGACGATCG
AAGGCGTGGTGGCCTCCGTGCTGCTGCTCGCCGGAGGCCTCAGCGCGCTGCAGACCATGGCCATCGCTGCCGGCCTGCCG
TTCTGCATCCTGATGATGCTGATCGCCTTGGGCATGTGGCGGGCGCTGGTGATCGAGGGGCATCAGCTGAGCCTGCAGAC
CCACGTGCAGACCGCCAACCGGCAGAGCGCAGCAGCCGGCCCGGGCGTGTGGAAGAAGCGCCTGGCCGGGCTGGTCACCT
TTCCCAGCCGCGAGGAGGTGGAAGGTTTCATGGCCAGTACCGTGCTCAAGGGCATGCGCCGCGTGCAGCGTGGTCTGACC
GAGCAGGGCTGGCCGGCCGAGGTCCAGGTAGACGAAGCCAATTCGCGCCTGTATCTGGAGGTGATCAAGGACGACCAGCT
CGACTTCGTCTACGAGATCCGCCTGATCAGCTACGCCATGCCGGCTTTCGCCCAGCCACAAAGCCCGGACGGCGACGAGC
ACTACTACCGCGCCGAGGTGTTCCTGCGCCGTGGTGGGCAGTCCTATGACATTTATGGCTATGACCAGGCAGAGATCATC
AGCGACATCCTCGATCAGTTCGAGAAGTACCTGCACTTCCTGCATATCTCGCCCGGCAGCCTGCCGTGGAAGATGGACGA
ACACGACGAAATGCTTGGTAACGAATCGGAGCCGCCGAAGGTCGCGCCGCAGTAG

Upstream 100 bases:

>100_bases
GCGCTTCGGGCCGTCTACCCAGAAGGCAGCGTTCGGGCAATCCGGAGGTTTCGCCGCGTACATCCCGCTCATCACCCGCT
CAAACGGAGGCTGACCGCCC

Downstream 100 bases:

>100_bases
GGGCCGGTCGACGGCGGCCCGGCGCAGACGATACGAAGCCGCCAGCGAAAACGGTGGTTGACGACAGATGGCAGCTTTTC
GATGCCCACAGCCCGTGCGC

Product: choline transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MDAARKLPKAIILFPVFIPAVVITLLLVIGTASNPELAGELFDSVLAYITRTFGWFYMLSVAFFLVFIVGIALTKWGNIK
LGPDHSEPQYSFPAWFAMLFSAGYGIALLFFGVAEPVLHYSTPPAGAPETVDAAKQAMQIAFFHWGFHIWGIFGIVGLAL
AYFAFRHGLPLSLRSALYPLIGERIYGPIGHFVDVLVILGTLFGIATTLGLSVTQINAGLNYLWDVPVDITVQVITIAII
TCMAIASVVAGLDKGVKNLSLLNIVLATVLMLFVLAVGPTIFILETFLQNTGSYLNNIVERTFNLQAYSRSDWIGNWTLF
IFGWTISWSPFVGLFIAKISRGRTIRQFVFGVMIVPTMFTFFWFSVFGDTALHLIMVEGYNTLIQDVQADHAVALFKLFE
LLPWTTLTSILAVLLIVTFFVTSSDSGSLVIDSIAAGGAIHTPTWQRVFWATIEGVVASVLLLAGGLSALQTMAIAAGLP
FCILMMLIALGMWRALVIEGHQLSLQTHVQTANRQSAAAGPGVWKKRLAGLVTFPSREEVEGFMASTVLKGMRRVQRGLT
EQGWPAEVQVDEANSRLYLEVIKDDQLDFVYEIRLISYAMPAFAQPQSPDGDEHYYRAEVFLRRGGQSYDIYGYDQAEII
SDILDQFEKYLHFLHISPGSLPWKMDEHDEMLGNESEPPKVAPQ

Sequences:

>Translated_684_residues
MDAARKLPKAIILFPVFIPAVVITLLLVIGTASNPELAGELFDSVLAYITRTFGWFYMLSVAFFLVFIVGIALTKWGNIK
LGPDHSEPQYSFPAWFAMLFSAGYGIALLFFGVAEPVLHYSTPPAGAPETVDAAKQAMQIAFFHWGFHIWGIFGIVGLAL
AYFAFRHGLPLSLRSALYPLIGERIYGPIGHFVDVLVILGTLFGIATTLGLSVTQINAGLNYLWDVPVDITVQVITIAII
TCMAIASVVAGLDKGVKNLSLLNIVLATVLMLFVLAVGPTIFILETFLQNTGSYLNNIVERTFNLQAYSRSDWIGNWTLF
IFGWTISWSPFVGLFIAKISRGRTIRQFVFGVMIVPTMFTFFWFSVFGDTALHLIMVEGYNTLIQDVQADHAVALFKLFE
LLPWTTLTSILAVLLIVTFFVTSSDSGSLVIDSIAAGGAIHTPTWQRVFWATIEGVVASVLLLAGGLSALQTMAIAAGLP
FCILMMLIALGMWRALVIEGHQLSLQTHVQTANRQSAAAGPGVWKKRLAGLVTFPSREEVEGFMASTVLKGMRRVQRGLT
EQGWPAEVQVDEANSRLYLEVIKDDQLDFVYEIRLISYAMPAFAQPQSPDGDEHYYRAEVFLRRGGQSYDIYGYDQAEII
SDILDQFEKYLHFLHISPGSLPWKMDEHDEMLGNESEPPKVAPQ
>Mature_684_residues
MDAARKLPKAIILFPVFIPAVVITLLLVIGTASNPELAGELFDSVLAYITRTFGWFYMLSVAFFLVFIVGIALTKWGNIK
LGPDHSEPQYSFPAWFAMLFSAGYGIALLFFGVAEPVLHYSTPPAGAPETVDAAKQAMQIAFFHWGFHIWGIFGIVGLAL
AYFAFRHGLPLSLRSALYPLIGERIYGPIGHFVDVLVILGTLFGIATTLGLSVTQINAGLNYLWDVPVDITVQVITIAII
TCMAIASVVAGLDKGVKNLSLLNIVLATVLMLFVLAVGPTIFILETFLQNTGSYLNNIVERTFNLQAYSRSDWIGNWTLF
IFGWTISWSPFVGLFIAKISRGRTIRQFVFGVMIVPTMFTFFWFSVFGDTALHLIMVEGYNTLIQDVQADHAVALFKLFE
LLPWTTLTSILAVLLIVTFFVTSSDSGSLVIDSIAAGGAIHTPTWQRVFWATIEGVVASVLLLAGGLSALQTMAIAAGLP
FCILMMLIALGMWRALVIEGHQLSLQTHVQTANRQSAAAGPGVWKKRLAGLVTFPSREEVEGFMASTVLKGMRRVQRGLT
EQGWPAEVQVDEANSRLYLEVIKDDQLDFVYEIRLISYAMPAFAQPQSPDGDEHYYRAEVFLRRGGQSYDIYGYDQAEII
SDILDQFEKYLHFLHISPGSLPWKMDEHDEMLGNESEPPKVAPQ

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=675, Percent_Identity=38.5185185185185, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI1786224, Length=484, Percent_Identity=27.2727272727273, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788102, Length=445, Percent_Identity=28.0898876404494, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 75613; Mature: 75613

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]