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The map label for this gene is ligB [H]

Identifier: 146284243

GI number: 146284243

Start: 4261120

End: 4262913

Strand: Direct

Name: ligB [H]

Synonym: PST_3931

Alternate gene names: 146284243

Gene position: 4261120-4262913 (Clockwise)

Preceding gene: 146284242

Following gene: 146284244

Centisome position: 93.29

GC content: 70.79

Gene sequence:

>1794_bases
GTGGGATTTCCATCAGGAACTGTTCAACATGCGCTGACGCACGGTCGGCTTGCGCCTGGCAGGCGACAATCCGGCATCGG
TCCGCCGGCGTCGGCGGGCCGCGCCCCCGTCACCAGAGCAAGGATGCTCGCCATGCCACGCCTGATCACGCTATCGCTGT
GCCTGTTCGCCCCTGCCTGCCTCGCCACCTGTCCGAACTGGGACGACGCGCGGGCACGCCAGGAAATCCACCAGCTGCAG
GCGCAGCTCGCCCAGTGGGACGATGCCTATCACCGGCGCGGTCAGTCGCTGGTGGACGACGAGATCTATGACCAGAGCCG
GGCGCAGCTGGACGGCTGGCGGCGCTGCTTCGTCGGCACCGGCGCGGCACCTGATCCGCTGCAGGATGCCGGCGGTCCGC
TGCGCCATCCGGTCAGACAGACCGGCCTGGTCAAGCTCGCCGACGAGCAGGCGGTTGCGACCTGGATCGCGCGGCGTCGC
GACCTGTGGATTCAGCCCAAGATCGACGGTGTTGCGGTCACCCTGGTCTATCGTCACGGCGCCCTGCAGCAGGCCATCAG
CCGTGGCGATGGCCTTACCGGACATGACTGGACAGCCAATGCCCGCAGACTGGCGGCGATCCCTGCGCGCCTGGCGGTAC
CCGACGAGGTCATCCTGCAGGGCGAGCTCTACTGGCGACTGGACCGGCATGTCCAGGCATCGCATGGCAGCGCGGGGGCT
CGCGGGCGCGTGGCCGGCGCCATGGCCAGCAACGGCCTGGACAGGAACACTGCCGCGCGGATCGGCCTGTTCGTCTGGGA
CTGGCCGAACGGCCCGGACAGCATGAACGCCCGACTCGGGCAGCTGGCGGCACTCGGCTTCCCCGATACGCAGGCGTTCA
GCCAGCCCGTGGAGACACTGGAACACGCCCGCCACTGGCGCGAGCACTGGTATCGCCAAGCGTTGCCGTTCGCCACCGAT
GGCGTGGTGCTGCGCCAGGAGCAACGTCCGCCGGCCGAGCGCTGGCAGGCCGAGCCCCACTGGGCCGCAGCCTGGAAGTA
CCCGCTGCGCAAGGCGCTGACCGAGGTGCGCGACGTCGAATTTCGCATCGGCCGCACCGGGCGGATCACACCGCTGCTGC
AGCTCGCTCCGGTCCAGCTGGATGATCGACGCGTGCGCATGCTGAGCCTCGGTTCGCTGGATCGTTGGCAGGCGCTGGAC
GTGCGCCCCGGTGACCGGGTCGCGGTTGCGCTGGCCGGGCATAGCATTCCGCAGCTGGACAGCGTGGTCTGGCGCCACAC
CGAGCGGGCTCCGGTCGCCGCGCCCGATCCGCGGCGCTATCACCCCGACAGCTGCTGGCGGCCAGCGCCCGGCTGCGAAC
AGCAGTTTCTCGCCCGTCTGGTCTGGCTGGGCGGCAGGCAAGGCTTGGCGCTCGAGGGCGTCGGCGCCGGCAGCTGGCAG
GCATTGCTGGAGGCCGGCCTGCTACCCGACCTGCTCGCCTGGCTGGAATTGGATGCCGCCGCACTCGAGCAGGTGCCCGG
CATTGGCAAGGCGCGCGCCAACAAGCTCGCCGCGAGCTTCGCCCGAGCCCGGCAGAGGCCGCTGGCGCAATGGCTGAAGG
CGCTCGGCCTGCCGGCCAAGCAGCTGCCGCCAGCAGCGGACTGGGACGCGCTCGCCGCACGTGACCTGGCGCAGTGGCAA
GCCGAAGCGGGAGTCGGTCCGGCGCGGGCGAAACAGCTGCAGGCGTTCTTCCGCGCGGCGGAGCTGCCCGCGCTGCGCGA
ACGACTACGCGCCGTCGGGGTTCCGGGCACCTGA

Upstream 100 bases:

>100_bases
AGCTGGCGCCGCAGTGGCAGGACAGTGACGTCTGGTTCTGCGGCCCTGCCGGTTTCGGTCAGGACCTCAGCCGTGACCTG
CAGCGGCGCGGCCTGCCGGC

Downstream 100 bases:

>100_bases
GGGACTTTCGCCCGAACCCTGCGGTCGATAGAAGCTGGATAACAACTATGGAGGCGTGGCATGTCTTTGCGACATCTGCC
CATTGCCCTGCTGACCGCCT

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MGFPSGTVQHALTHGRLAPGRRQSGIGPPASAGRAPVTRARMLAMPRLITLSLCLFAPACLATCPNWDDARARQEIHQLQ
AQLAQWDDAYHRRGQSLVDDEIYDQSRAQLDGWRRCFVGTGAAPDPLQDAGGPLRHPVRQTGLVKLADEQAVATWIARRR
DLWIQPKIDGVAVTLVYRHGALQQAISRGDGLTGHDWTANARRLAAIPARLAVPDEVILQGELYWRLDRHVQASHGSAGA
RGRVAGAMASNGLDRNTAARIGLFVWDWPNGPDSMNARLGQLAALGFPDTQAFSQPVETLEHARHWREHWYRQALPFATD
GVVLRQEQRPPAERWQAEPHWAAAWKYPLRKALTEVRDVEFRIGRTGRITPLLQLAPVQLDDRRVRMLSLGSLDRWQALD
VRPGDRVAVALAGHSIPQLDSVVWRHTERAPVAAPDPRRYHPDSCWRPAPGCEQQFLARLVWLGGRQGLALEGVGAGSWQ
ALLEAGLLPDLLAWLELDAAALEQVPGIGKARANKLAASFARARQRPLAQWLKALGLPAKQLPPAADWDALAARDLAQWQ
AEAGVGPARAKQLQAFFRAAELPALRERLRAVGVPGT

Sequences:

>Translated_597_residues
MGFPSGTVQHALTHGRLAPGRRQSGIGPPASAGRAPVTRARMLAMPRLITLSLCLFAPACLATCPNWDDARARQEIHQLQ
AQLAQWDDAYHRRGQSLVDDEIYDQSRAQLDGWRRCFVGTGAAPDPLQDAGGPLRHPVRQTGLVKLADEQAVATWIARRR
DLWIQPKIDGVAVTLVYRHGALQQAISRGDGLTGHDWTANARRLAAIPARLAVPDEVILQGELYWRLDRHVQASHGSAGA
RGRVAGAMASNGLDRNTAARIGLFVWDWPNGPDSMNARLGQLAALGFPDTQAFSQPVETLEHARHWREHWYRQALPFATD
GVVLRQEQRPPAERWQAEPHWAAAWKYPLRKALTEVRDVEFRIGRTGRITPLLQLAPVQLDDRRVRMLSLGSLDRWQALD
VRPGDRVAVALAGHSIPQLDSVVWRHTERAPVAAPDPRRYHPDSCWRPAPGCEQQFLARLVWLGGRQGLALEGVGAGSWQ
ALLEAGLLPDLLAWLELDAAALEQVPGIGKARANKLAASFARARQRPLAQWLKALGLPAKQLPPAADWDALAARDLAQWQ
AEAGVGPARAKQLQAFFRAAELPALRERLRAVGVPGT
>Mature_596_residues
GFPSGTVQHALTHGRLAPGRRQSGIGPPASAGRAPVTRARMLAMPRLITLSLCLFAPACLATCPNWDDARARQEIHQLQA
QLAQWDDAYHRRGQSLVDDEIYDQSRAQLDGWRRCFVGTGAAPDPLQDAGGPLRHPVRQTGLVKLADEQAVATWIARRRD
LWIQPKIDGVAVTLVYRHGALQQAISRGDGLTGHDWTANARRLAAIPARLAVPDEVILQGELYWRLDRHVQASHGSAGAR
GRVAGAMASNGLDRNTAARIGLFVWDWPNGPDSMNARLGQLAALGFPDTQAFSQPVETLEHARHWREHWYRQALPFATDG
VVLRQEQRPPAERWQAEPHWAAAWKYPLRKALTEVRDVEFRIGRTGRITPLLQLAPVQLDDRRVRMLSLGSLDRWQALDV
RPGDRVAVALAGHSIPQLDSVVWRHTERAPVAAPDPRRYHPDSCWRPAPGCEQQFLARLVWLGGRQGLALEGVGAGSWQA
LLEAGLLPDLLAWLELDAAALEQVPGIGKARANKLAASFARARQRPLAQWLKALGLPAKQLPPAADWDALAARDLAQWQA
EAGVGPARAKQLQAFFRAAELPALRERLRAVGVPGT

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=556, Percent_Identity=38.6690647482014, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1788750, Length=566, Percent_Identity=28.4452296819788, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 65712; Mature: 65580

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFPSGTVQHALTHGRLAPGRRQSGIGPPASAGRAPVTRARMLAMPRLITLSLCLFAPAC
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LATCPNWDDARARQEIHQLQAQLAQWDDAYHRRGQSLVDDEIYDQSRAQLDGWRRCFVGT
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECC
GAAPDPLQDAGGPLRHPVRQTGLVKLADEQAVATWIARRRDLWIQPKIDGVAVTLVYRHG
CCCCCCHHHCCCCHHCCHHHCCCEEECCHHHHHHHHHHHHCCEECCCCCCEEEEEEEHHH
ALQQAISRGDGLTGHDWTANARRLAAIPARLAVPDEVILQGELYWRLDRHVQASHGSAGA
HHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHCCCHHHEECCHHEEEEHHHHCCCCCCCCC
RGRVAGAMASNGLDRNTAARIGLFVWDWPNGPDSMNARLGQLAALGFPDTQAFSQPVETL
CCHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
EHARHWREHWYRQALPFATDGVVLRQEQRPPAERWQAEPHWAAAWKYPLRKALTEVRDVE
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FRIGRTGRITPLLQLAPVQLDDRRVRMLSLGSLDRWQALDVRPGDRVAVALAGHSIPQLD
HEECCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEECCCCCHHHH
SVVWRHTERAPVAAPDPRRYHPDSCWRPAPGCEQQFLARLVWLGGRQGLALEGVGAGSWQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHH
ALLEAGLLPDLLAWLELDAAALEQVPGIGKARANKLAASFARARQRPLAQWLKALGLPAK
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLPPAADWDALAARDLAQWQAEAGVGPARAKQLQAFFRAAELPALRERLRAVGVPGT
HCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GFPSGTVQHALTHGRLAPGRRQSGIGPPASAGRAPVTRARMLAMPRLITLSLCLFAPAC
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LATCPNWDDARARQEIHQLQAQLAQWDDAYHRRGQSLVDDEIYDQSRAQLDGWRRCFVGT
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECC
GAAPDPLQDAGGPLRHPVRQTGLVKLADEQAVATWIARRRDLWIQPKIDGVAVTLVYRHG
CCCCCCHHHCCCCHHCCHHHCCCEEECCHHHHHHHHHHHHCCEECCCCCCEEEEEEEHHH
ALQQAISRGDGLTGHDWTANARRLAAIPARLAVPDEVILQGELYWRLDRHVQASHGSAGA
HHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHCCCHHHEECCHHEEEEHHHHCCCCCCCCC
RGRVAGAMASNGLDRNTAARIGLFVWDWPNGPDSMNARLGQLAALGFPDTQAFSQPVETL
CCHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
EHARHWREHWYRQALPFATDGVVLRQEQRPPAERWQAEPHWAAAWKYPLRKALTEVRDVE
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FRIGRTGRITPLLQLAPVQLDDRRVRMLSLGSLDRWQALDVRPGDRVAVALAGHSIPQLD
HEECCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEECCCCCHHHH
SVVWRHTERAPVAAPDPRRYHPDSCWRPAPGCEQQFLARLVWLGGRQGLALEGVGAGSWQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHH
ALLEAGLLPDLLAWLELDAAALEQVPGIGKARANKLAASFARARQRPLAQWLKALGLPAK
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLPPAADWDALAARDLAQWQAEAGVGPARAKQLQAFFRAAELPALRERLRAVGVPGT
HCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA