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The map label for this gene is aceF [H]

Identifier: 146284165

GI number: 146284165

Start: 4171663

End: 4173327

Strand: Direct

Name: aceF [H]

Synonym: PST_3853

Alternate gene names: 146284165

Gene position: 4171663-4173327 (Clockwise)

Preceding gene: 146284164

Following gene: 146284167

Centisome position: 91.34

GC content: 66.31

Gene sequence:

>1665_bases
GTGAGTGAAACCATACGCGTACCCGACATCGGCAGCGGTGAGGGTGAAGTGATCGAGTTGTTCGTCAAGGTCGGCGACCG
TATCGAAGCCGACCAGAGCATCCTGACGCTCGAGTCCGACAAGGCCAGCATGGAAATCCCGGCGCCCAAGGCAGGCGTCG
TGAAAGCCTTGAAGGTCAAGCTGGGCGATCGCCTGAAGGAAGGCGACGAACTGCTGGAGCTGGAAAGCGAAGGTGGCGAA
GCCGCTGCCGAAGCACCCGCGGCGCCTGCCGCGGAGGCCGCGCAACCGAGCCAGGCGCCCGATGAAGCCGAAGCCACTGA
AGCCCCTGCGGCCAGCGAAGCACCGGCCGCAGCAGGCGCCGAACAGAGCCAGGAGATCCGCGTGCCGGATATCGGCTCGG
CCGGCAAGGCCAACGTCATCGAGATCATGGTCAAGGCGGGTGATTCGGTACAGGCCGAGCAGTCGCTGATCACCCTGGAA
TCGGACAAGGCCAGCATGGAGATTCCGTCTCCAGTGGCCGGCGTGGTAGAGAGCCTGGCGATCAAGCTGAACGACGAGGT
CGGCACCGGTGATCTGATCCTGACCCTCAAGGTCCAGGGCGCGGCACCGGCCAAGCAGGCCGAGTCGACGCCGGCGCCGA
GTCCCGCCGAGCCGGCCAAGGCCGCAAGCCCAGAACAAGCCGACCGCAAGCCGGCACCAACCGCGCAGAGCGAAGCTCCA
CAGGCGGTCGGCGCACCGAGCCGCGACGGCGCCAAGGTGCATGCCGGCCCGGCCGTGCGCCAGCTGGCCCGCGAGTTCGG
CGTCGAGCTGAGCGCGGTCGCCGGCAGCGGTCCGAAGGGACGCATCCTCAAGGAAGACGTGCAGCAGTATGTGAAGACCA
TGCTGCAGCAGGGCAATGCCGAACCGACCGCGGCCGCGGCCGGTGGAGCCGGCATTCCGCCGATCCCGGCGGTGGACTTC
AGCAAGTTCGGCGAGGTCGAGGAAGTCGCGATGTCGCGACTGATGCAGATCGGCGCCGCCAACCTGCATCGCAGCTGGCT
GAACGTGCCGCACGTGACCCAGTTCGAGTCGGCCGACATTACCGAGCTGGAAGCTTTCCGCGTTGCGCAGAAGGCGATCG
CCGAGAAGGCTGGGGTCAAGCTCACCGTGCTGCCGCTATTGCTCAAGGCGTGCGCGCACCTGCTAAAGGAACTGCCGGAT
TTCAACAGTTCCCTGGCCCCGAGCGGCAAGGCGCTGATCCGCAAGAAGTATGTGCACGTAGGGTTCGCCGTCGACACGCC
GGATGGCCTGATGGTCCCGGTGATTCGCAACGTCGACCAGAAGAGTCTGCTGCAACTGGCCGGCGAGGCTGCGGAGCTGG
CTGAGAAGGCCCGCACCAAGAAGCTTTCTCCGGATGCGATGCAGGGCGCCTGCTTCACGATCTCCAGCCTCGGCCACATC
GGCGGCACCGGCTTCACGCCGATCGTCAATGCGCCGGAAGTGGCGATCCTAGGCGTCTCCAAGGCGGCCATGCAGCCGGT
GTGGGATGGCAAGGCCTTCCAGCCCCGCCTGATGCTGCCGCTGTCGCTCTCCTACGACCACCGGGTGATCAACGGTGCCG
CGGCGGCGCGCTTCACCAAGCGCCTGTCGGAGCTGCTGGCCGATATCCGCACCATGCTGCTGTAA

Upstream 100 bases:

>100_bases
ACGCAAGGTCGTGGCCGAGGCCATCACCGAGTTCGGTATCGACCCCGAGAAGCGCAATCCGCTGGATTGCTGATGCTGCG
TGCGCACAGGAGAACCTATT

Downstream 100 bases:

>100_bases
CGGCGCGCGAAAAAAACGCCGCGTTCTCACAGAGAGACCGCGGCGTTTTTTATTGAGCGGGAAAAAGGTGAAGGCTTGGT
CGCGGCAGACGAACCCTAGT

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSETIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEIPAPKAGVVKALKVKLGDRLKEGDELLELESEGGE
AAAEAPAAPAAEAAQPSQAPDEAEATEAPAASEAPAAAGAEQSQEIRVPDIGSAGKANVIEIMVKAGDSVQAEQSLITLE
SDKASMEIPSPVAGVVESLAIKLNDEVGTGDLILTLKVQGAAPAKQAESTPAPSPAEPAKAASPEQADRKPAPTAQSEAP
QAVGAPSRDGAKVHAGPAVRQLAREFGVELSAVAGSGPKGRILKEDVQQYVKTMLQQGNAEPTAAAAGGAGIPPIPAVDF
SKFGEVEEVAMSRLMQIGAANLHRSWLNVPHVTQFESADITELEAFRVAQKAIAEKAGVKLTVLPLLLKACAHLLKELPD
FNSSLAPSGKALIRKKYVHVGFAVDTPDGLMVPVIRNVDQKSLLQLAGEAAELAEKARTKKLSPDAMQGACFTISSLGHI
GGTGFTPIVNAPEVAILGVSKAAMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTKRLSELLADIRTMLL

Sequences:

>Translated_554_residues
MSETIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEIPAPKAGVVKALKVKLGDRLKEGDELLELESEGGE
AAAEAPAAPAAEAAQPSQAPDEAEATEAPAASEAPAAAGAEQSQEIRVPDIGSAGKANVIEIMVKAGDSVQAEQSLITLE
SDKASMEIPSPVAGVVESLAIKLNDEVGTGDLILTLKVQGAAPAKQAESTPAPSPAEPAKAASPEQADRKPAPTAQSEAP
QAVGAPSRDGAKVHAGPAVRQLAREFGVELSAVAGSGPKGRILKEDVQQYVKTMLQQGNAEPTAAAAGGAGIPPIPAVDF
SKFGEVEEVAMSRLMQIGAANLHRSWLNVPHVTQFESADITELEAFRVAQKAIAEKAGVKLTVLPLLLKACAHLLKELPD
FNSSLAPSGKALIRKKYVHVGFAVDTPDGLMVPVIRNVDQKSLLQLAGEAAELAEKARTKKLSPDAMQGACFTISSLGHI
GGTGFTPIVNAPEVAILGVSKAAMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTKRLSELLADIRTMLL
>Mature_553_residues
SETIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEIPAPKAGVVKALKVKLGDRLKEGDELLELESEGGEA
AAEAPAAPAAEAAQPSQAPDEAEATEAPAASEAPAAAGAEQSQEIRVPDIGSAGKANVIEIMVKAGDSVQAEQSLITLES
DKASMEIPSPVAGVVESLAIKLNDEVGTGDLILTLKVQGAAPAKQAESTPAPSPAEPAKAASPEQADRKPAPTAQSEAPQ
AVGAPSRDGAKVHAGPAVRQLAREFGVELSAVAGSGPKGRILKEDVQQYVKTMLQQGNAEPTAAAAGGAGIPPIPAVDFS
KFGEVEEVAMSRLMQIGAANLHRSWLNVPHVTQFESADITELEAFRVAQKAIAEKAGVKLTVLPLLLKACAHLLKELPDF
NSSLAPSGKALIRKKYVHVGFAVDTPDGLMVPVIRNVDQKSLLQLAGEAAELAEKARTKKLSPDAMQGACFTISSLGHIG
GTGFTPIVNAPEVAILGVSKAAMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTKRLSELLADIRTMLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=461, Percent_Identity=30.8026030368764, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI31711992, Length=326, Percent_Identity=31.9018404907975, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=34.6666666666667, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI203098816, Length=483, Percent_Identity=25.879917184265, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI203098753, Length=439, Percent_Identity=26.1958997722096, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI260898739, Length=151, Percent_Identity=33.112582781457, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786305, Length=552, Percent_Identity=49.6376811594203, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=28.110599078341, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=29.0993071593533, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=29.7539149888143, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25146366, Length=426, Percent_Identity=29.3427230046948, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=30.3225806451613, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=30.5045871559633, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=355, Percent_Identity=31.2676056338028, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18859875, Length=445, Percent_Identity=28.0898876404494, Blast_Score=162, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=31.3304721030043, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24645909, Length=217, Percent_Identity=33.1797235023041, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=31.3304721030043, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57613; Mature: 57482

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEIPAPKAGVVKALKVK
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHH
LGDRLKEGDELLELESEGGEAAAEAPAAPAAEAAQPSQAPDEAEATEAPAASEAPAAAGA
HHHHHHCCCHHHEECCCCCCHHHCCCCCCCHHCCCCCCCCCHHHHCCCCCCCCCCCCCCC
EQSQEIRVPDIGSAGKANVIEIMVKAGDSVQAEQSLITLESDKASMEIPSPVAGVVESLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHEEEEECCCCCCCCCCHHHHHHHHHH
IKLNDEVGTGDLILTLKVQGAAPAKQAESTPAPSPAEPAKAASPEQADRKPAPTAQSEAP
EEECCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
QAVGAPSRDGAKVHAGPAVRQLAREFGVELSAVAGSGPKGRILKEDVQQYVKTMLQQGNA
CCCCCCCCCCCEEECCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
EPTAAAAGGAGIPPIPAVDFSKFGEVEEVAMSRLMQIGAANLHRSWLNVPHVTQFESADI
CCCHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TELEAFRVAQKAIAEKAGVKLTVLPLLLKACAHLLKELPDFNSSLAPSGKALIRKKYVHV
HHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEE
GFAVDTPDGLMVPVIRNVDQKSLLQLAGEAAELAEKARTKKLSPDAMQGACFTISSLGHI
EEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCC
GGTGFTPIVNAPEVAILGVSKAAMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTK
CCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
RLSELLADIRTMLL
HHHHHHHHHHHHHC
>Mature Secondary Structure 
SETIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEIPAPKAGVVKALKVK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHH
LGDRLKEGDELLELESEGGEAAAEAPAAPAAEAAQPSQAPDEAEATEAPAASEAPAAAGA
HHHHHHCCCHHHEECCCCCCHHHCCCCCCCHHCCCCCCCCCHHHHCCCCCCCCCCCCCCC
EQSQEIRVPDIGSAGKANVIEIMVKAGDSVQAEQSLITLESDKASMEIPSPVAGVVESLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHEEEEECCCCCCCCCCHHHHHHHHHH
IKLNDEVGTGDLILTLKVQGAAPAKQAESTPAPSPAEPAKAASPEQADRKPAPTAQSEAP
EEECCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
QAVGAPSRDGAKVHAGPAVRQLAREFGVELSAVAGSGPKGRILKEDVQQYVKTMLQQGNA
CCCCCCCCCCCEEECCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
EPTAAAAGGAGIPPIPAVDFSKFGEVEEVAMSRLMQIGAANLHRSWLNVPHVTQFESADI
CCCHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TELEAFRVAQKAIAEKAGVKLTVLPLLLKACAHLLKELPDFNSSLAPSGKALIRKKYVHV
HHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEE
GFAVDTPDGLMVPVIRNVDQKSLLQLAGEAAELAEKARTKKLSPDAMQGACFTISSLGHI
EEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCC
GGTGFTPIVNAPEVAILGVSKAAMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTK
CCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
RLSELLADIRTMLL
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171401; 10984043 [H]