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The map label for this gene is qacF [H]

Identifier: 146284131

GI number: 146284131

Start: 4131885

End: 4132244

Strand: Direct

Name: qacF [H]

Synonym: PST_3819

Alternate gene names: 146284131

Gene position: 4131885-4132244 (Clockwise)

Preceding gene: 146284129

Following gene: 146284132

Centisome position: 90.46

GC content: 63.89

Gene sequence:

>360_bases
TTGCCCGCCCATAATCAGGATCTCGCCATGTCCGGCTATGTTTACCTGGCCTTCGCCATCTGCGCCGAGGTGGTCGCCAC
CACCTCGATGAAGGCACTGGCCGGCTTCAATCGTCCGCTGCCACTGCTGCTGGTGGTGACGGGCTACAGCCTGTCGTTCT
GGATGCTCGCGCTGGTGGTACGGACCATCCCGGTAGGCATCGCCTATGCCATCTGGGCCGGGCTCGGCATCGTGCTGGTC
AGCGTCGCCGCGGCGGTGCTCTATCGTCAGTACCTGGACCTGCCGGCGGTACTGGGCATGGGGCTGATCATCGCCGGTGT
GGTGGTCATCCAGTTGTTCTCCCGCAGTGCAGGCCACTAA

Upstream 100 bases:

>100_bases
GAGGGTGCGATTCATGGGCGCGGAGCTTAGCAAGCTGCCTGCTCGCGAGCCATAACCGCCCTGCCGGCGAACTTGTTGGC
GAGGTCGGAACCGAACGAAG

Downstream 100 bases:

>100_bases
GCTGCCGGCGGACTGCAGTTATACTGCGCGACCGTTTACCCAATGAGGTCTGCACATGCCCGAATCCCTCAGCACCGACG
TCCTGATCGTTGGCGGCGGC

Product: multidrug efflux SMR transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Quaternary ammonium determinant F [H]

Number of amino acids: Translated: 119; Mature: 118

Protein sequence:

>119_residues
MPAHNQDLAMSGYVYLAFAICAEVVATTSMKALAGFNRPLPLLLVVTGYSLSFWMLALVVRTIPVGIAYAIWAGLGIVLV
SVAAAVLYRQYLDLPAVLGMGLIIAGVVVIQLFSRSAGH

Sequences:

>Translated_119_residues
MPAHNQDLAMSGYVYLAFAICAEVVATTSMKALAGFNRPLPLLLVVTGYSLSFWMLALVVRTIPVGIAYAIWAGLGIVLV
SVAAAVLYRQYLDLPAVLGMGLIIAGVVVIQLFSRSAGH
>Mature_118_residues
PAHNQDLAMSGYVYLAFAICAEVVATTSMKALAGFNRPLPLLLVVTGYSLSFWMLALVVRTIPVGIAYAIWAGLGIVLVS
VAAAVLYRQYLDLPAVLGMGLIIAGVVVIQLFSRSAGH

Specific function: Multidrug exporter. Is implicated for the resistance to bacteriocidal quaternary ammonium compounds [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=110, Percent_Identity=51.8181818181818, Blast_Score=105, Evalue=6e-25,
Organism=Escherichia coli, GI1787884, Length=107, Percent_Identity=33.6448598130841, Blast_Score=62, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 12594; Mature: 12463

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHNQDLAMSGYVYLAFAICAEVVATTSMKALAGFNRPLPLLLVVTGYSLSFWMLALVV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHH
RTIPVGIAYAIWAGLGIVLVSVAAAVLYRQYLDLPAVLGMGLIIAGVVVIQLFSRSAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAHNQDLAMSGYVYLAFAICAEVVATTSMKALAGFNRPLPLLLVVTGYSLSFWMLALVV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHH
RTIPVGIAYAIWAGLGIVLVSVAAAVLYRQYLDLPAVLGMGLIIAGVVVIQLFSRSAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9756755 [H]