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The map label for this gene is mltB [H]

Identifier: 146284084

GI number: 146284084

Start: 4080512

End: 4081852

Strand: Direct

Name: mltB [H]

Synonym: PST_3772

Alternate gene names: 146284084

Gene position: 4080512-4081852 (Clockwise)

Preceding gene: 146284083

Following gene: 146284098

Centisome position: 89.34

GC content: 67.49

Gene sequence:

>1341_bases
GTGATAATGTCAGCGGCCGACGACACCTTTCGAGAGTGGTGCATGTATTACACCTTCGTTCCCGTTCTGCTGCTGCGAAC
GCTGGTCGCCGCCTCGGCGATGAGCCTGGTGGTCGCCTGTGCCGCCGAACCCCTCGCGCAGTCTTCTCTTGTCGGCAACG
CGGCCGCCCCCTCCGGTCAGCGTAGCGACAATGCCGTCGTCGACGCGCCAGCAGAAGCCACGCATCTCAGCTTCAGCCAG
TGGCGCGAGCAGTTCCGTGCAGAAGCCCTGGCAGCGGGCATTTCCTCCACCACCTTCGACCGCGCCTTCGCCGGGGTGCA
GCCCGATCCGGCGGTGATCGAAGCCGATCGCAGCCAGCCGGAGTTCACGCGCCCGGTCTGGCAATATCTGGAAAGCGCGA
TTTCGCCCCAGCGGGTCCGCAGCGGACAGCGCCTGTTGAACGAGCACGCCGCAACCCTGGACCGCATCGAGGCACGCTAC
GGCGTCGATCGCGAAACCCTGGTCGCCGTCTGGGGCCTTGAGAGCAGTTTCGGCCAGATCATGGGCGACAAGTCGGTGAT
CCGCTCGCTGGCCACCCTCGCCCATGAAGGGCGCCGCCCGGCCTTTGCCAAGAGCCAGCTGATCGCGGCGTTGGAGATCC
TCCAGCACGGCGACGTGTCACCGCAGCAGATGCGCGGTTCCTGGGCTGGCGCCATGGGTCAGACGCAGTTCATCCCGACC
ACCTACAACACCCATGCGGTGGACTTCGACGGTGACGGCAAGCGCGACATCTGGAACAGCTCGGCCGATGCCCTGGCGTC
CGCCGCACACTACCTGCAGGCCTCTGGCTGGAAGCAAGGTCAGCCCTGGGGCTTCGAGGTGCGACTGCCCAGCGGCTTCG
ACTACGCCCTGGCCGACAACGAGATCCGCAAGCCACTGGCGGAATGGCGCCGCCTCGGCCTACGCGGCCTGCCCGGCGGA
CGAGACGAAGCCAATGCCAGCCTGCTGCTGCCAGCCGGACACCGCGGTCCGGCATTCCTGATCATGGACAATTTCCGCGC
GATCCTGCGTTACAACAACTCCTCGGCCTACGCGCTGGCCATCGGGCTGCTGTCCGAGAGCTTCCAGGGCAAGGGCCAGG
TGGCGGGTAGTTGGCCGCGTGGCGAGCAGCCGCTCAGCCGTTCGGAGCGCCTGGAGCTGCAGGAGCGCCTCGTCGCTCAG
GGCTTCGACCCCGGCACCCCGGACGGCATCATCGGTGCCAATACGCGCAAGGCCATCCGCGGCTTCCAGCAGCGCCTCGG
CTGGCCCGCCGACGGTCATCCCACCCAGGAACTGCTCGGCCGCCTGCGCGCTCAGCCCTGA

Upstream 100 bases:

>100_bases
CCAAGAAAGGCAAAGGCAGCTACCGCCGCGAAGCCTCCCAGTCCACCAACTGGGAGGCTTCGGTCCTTCTGACGGCCTGA
AAACCCAACACCCCGAAAGC

Downstream 100 bases:

>100_bases
CTAACCTCAGCCAACGAAAAAAGCCCCGGCAGGTTATGCCGGGGCTTTTTTTTGCGTTCAGCCTCTACGTGAGTGCCAGA
CGGCCTCAGTGCTTGATCGC

Product: membrane-bound lytic murein transglycosylase, putative

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MIMSAADDTFREWCMYYTFVPVLLLRTLVAASAMSLVVACAAEPLAQSSLVGNAAAPSGQRSDNAVVDAPAEATHLSFSQ
WREQFRAEALAAGISSTTFDRAFAGVQPDPAVIEADRSQPEFTRPVWQYLESAISPQRVRSGQRLLNEHAATLDRIEARY
GVDRETLVAVWGLESSFGQIMGDKSVIRSLATLAHEGRRPAFAKSQLIAALEILQHGDVSPQQMRGSWAGAMGQTQFIPT
TYNTHAVDFDGDGKRDIWNSSADALASAAHYLQASGWKQGQPWGFEVRLPSGFDYALADNEIRKPLAEWRRLGLRGLPGG
RDEANASLLLPAGHRGPAFLIMDNFRAILRYNNSSAYALAIGLLSESFQGKGQVAGSWPRGEQPLSRSERLELQERLVAQ
GFDPGTPDGIIGANTRKAIRGFQQRLGWPADGHPTQELLGRLRAQP

Sequences:

>Translated_446_residues
MIMSAADDTFREWCMYYTFVPVLLLRTLVAASAMSLVVACAAEPLAQSSLVGNAAAPSGQRSDNAVVDAPAEATHLSFSQ
WREQFRAEALAAGISSTTFDRAFAGVQPDPAVIEADRSQPEFTRPVWQYLESAISPQRVRSGQRLLNEHAATLDRIEARY
GVDRETLVAVWGLESSFGQIMGDKSVIRSLATLAHEGRRPAFAKSQLIAALEILQHGDVSPQQMRGSWAGAMGQTQFIPT
TYNTHAVDFDGDGKRDIWNSSADALASAAHYLQASGWKQGQPWGFEVRLPSGFDYALADNEIRKPLAEWRRLGLRGLPGG
RDEANASLLLPAGHRGPAFLIMDNFRAILRYNNSSAYALAIGLLSESFQGKGQVAGSWPRGEQPLSRSERLELQERLVAQ
GFDPGTPDGIIGANTRKAIRGFQQRLGWPADGHPTQELLGRLRAQP
>Mature_446_residues
MIMSAADDTFREWCMYYTFVPVLLLRTLVAASAMSLVVACAAEPLAQSSLVGNAAAPSGQRSDNAVVDAPAEATHLSFSQ
WREQFRAEALAAGISSTTFDRAFAGVQPDPAVIEADRSQPEFTRPVWQYLESAISPQRVRSGQRLLNEHAATLDRIEARY
GVDRETLVAVWGLESSFGQIMGDKSVIRSLATLAHEGRRPAFAKSQLIAALEILQHGDVSPQQMRGSWAGAMGQTQFIPT
TYNTHAVDFDGDGKRDIWNSSADALASAAHYLQASGWKQGQPWGFEVRLPSGFDYALADNEIRKPLAEWRRLGLRGLPGG
RDEANASLLLPAGHRGPAFLIMDNFRAILRYNNSSAYALAIGLLSESFQGKGQVAGSWPRGEQPLSRSERLELQERLVAQ
GFDPGTPDGIIGANTRKAIRGFQQRLGWPADGHPTQELLGRLRAQP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=247, Percent_Identity=31.1740890688259, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 48655; Mature: 48655

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMSAADDTFREWCMYYTFVPVLLLRTLVAASAMSLVVACAAEPLAQSSLVGNAAAPSGQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RSDNAVVDAPAEATHLSFSQWREQFRAEALAAGISSTTFDRAFAGVQPDPAVIEADRSQP
CCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCCCCC
EFTRPVWQYLESAISPQRVRSGQRLLNEHAATLDRIEARYGVDRETLVAVWGLESSFGQI
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHH
MGDKSVIRSLATLAHEGRRPAFAKSQLIAALEILQHGDVSPQQMRGSWAGAMGQTQFIPT
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCCCCCEECCC
TYNTHAVDFDGDGKRDIWNSSADALASAAHYLQASGWKQGQPWGFEVRLPSGFDYALADN
CCCCEEEECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHCCH
EIRKPLAEWRRLGLRGLPGGRDEANASLLLPAGHRGPAFLIMDNFRAILRYNNSSAYALA
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCEEEEEECCCCEEEHH
IGLLSESFQGKGQVAGSWPRGEQPLSRSERLELQERLVAQGFDPGTPDGIIGANTRKAIR
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
GFQQRLGWPADGHPTQELLGRLRAQP
HHHHHCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIMSAADDTFREWCMYYTFVPVLLLRTLVAASAMSLVVACAAEPLAQSSLVGNAAAPSGQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RSDNAVVDAPAEATHLSFSQWREQFRAEALAAGISSTTFDRAFAGVQPDPAVIEADRSQP
CCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCCCCC
EFTRPVWQYLESAISPQRVRSGQRLLNEHAATLDRIEARYGVDRETLVAVWGLESSFGQI
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHH
MGDKSVIRSLATLAHEGRRPAFAKSQLIAALEILQHGDVSPQQMRGSWAGAMGQTQFIPT
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCCCCCEECCC
TYNTHAVDFDGDGKRDIWNSSADALASAAHYLQASGWKQGQPWGFEVRLPSGFDYALADN
CCCCEEEECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHCCH
EIRKPLAEWRRLGLRGLPGGRDEANASLLLPAGHRGPAFLIMDNFRAILRYNNSSAYALA
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCEEEEEECCCCEEEHH
IGLLSESFQGKGQVAGSWPRGEQPLSRSERLELQERLVAQGFDPGTPDGIIGANTRKAIR
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
GFQQRLGWPADGHPTQELLGRLRAQP
HHHHHCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]