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The map label for this gene is thiE

Identifier: 146284069

GI number: 146284069

Start: 4065585

End: 4066226

Strand: Direct

Name: thiE

Synonym: PST_3757

Alternate gene names: 146284069

Gene position: 4065585-4066226 (Clockwise)

Preceding gene: 146284068

Following gene: 146284070

Centisome position: 89.01

GC content: 68.54

Gene sequence:

>642_bases
ATGAAGGAATCGTCCCGCCTGCGCGGCCTGTACGCCATCACCGACAGCAAATTGCTGGCCGATGGCCGCCTGCTGCCCTA
TGTCGAGGCGGCACTGAAGGGCGGCGCCCGCCTGCTGCAATATCGAGACAAGACCAGCGACGAGGCGCGGCGGCTGCGCG
AGGCCGATGCACTGCAGGAGCTCTGCGCCCGCCACGGTGCTCAGCTGATCATCAACGATGATGCCGAGCTGGCCGCGCGC
CTGGGCGTAGGCCTGCACCTGGGCCAGGAAGATGGTTCGCTGGCAGCCGCCCGGGCCCTGCTCGGCCGCCAGGCGATCAT
CGGTGCCACCTGTCATGCGCAACTGCCGCTGGCCGAGCAGGCGGCGCGTGACGGCGCCAGCTACGTCGCCTTCGGCCGTT
TCTTCCAGTCCCACACCAAACCCGGCGCACCGGCAGCCAATCACGAATTGCTGCGTGAAGCCCGCGCCCGTATCGGCCTG
CCCATCGTCGCCATCGGTGGCATCACGCTGGACACCGCGCCATCACTGATCGCCGAAGGCGTGCAGATGATCGCCGTGAT
CCACGCCCTGTTCGCCGCCGACAGCGCCGCCGAGGTCGAGCGCCGCGCCCAGGCCTTCAGCCAACTGTTCAACACGCCCT
GA

Upstream 100 bases:

>100_bases
ACTACACCTGGCGCACGCTGCGCGATGCCGAGGCCCCCGGCCACGGCCAGTACGTGCCGCGCCGCCTGCCCCTGGACCTG
GCCTGACTGGAGACCGGCGC

Downstream 100 bases:

>100_bases
TTCCGCCACGGCGGGTCGCTGATGCCGGCCCGCCCCTGTTTTGCGAGGCCCGCATGTCCCGTTCCGAAACCCTCTTTGCC
AGCGCCCAGACCCACATCCC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKESSRLRGLYAITDSKLLADGRLLPYVEAALKGGARLLQYRDKTSDEARRLREADALQELCARHGAQLIINDDAELAAR
LGVGLHLGQEDGSLAAARALLGRQAIIGATCHAQLPLAEQAARDGASYVAFGRFFQSHTKPGAPAANHELLREARARIGL
PIVAIGGITLDTAPSLIAEGVQMIAVIHALFAADSAAEVERRAQAFSQLFNTP

Sequences:

>Translated_213_residues
MKESSRLRGLYAITDSKLLADGRLLPYVEAALKGGARLLQYRDKTSDEARRLREADALQELCARHGAQLIINDDAELAAR
LGVGLHLGQEDGSLAAARALLGRQAIIGATCHAQLPLAEQAARDGASYVAFGRFFQSHTKPGAPAANHELLREARARIGL
PIVAIGGITLDTAPSLIAEGVQMIAVIHALFAADSAAEVERRAQAFSQLFNTP
>Mature_213_residues
MKESSRLRGLYAITDSKLLADGRLLPYVEAALKGGARLLQYRDKTSDEARRLREADALQELCARHGAQLIINDDAELAAR
LGVGLHLGQEDGSLAAARALLGRQAIIGATCHAQLPLAEQAARDGASYVAFGRFFQSHTKPGAPAANHELLREARARIGL
PIVAIGGITLDTAPSLIAEGVQMIAVIHALFAADSAAEVERRAQAFSQLFNTP

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=193, Percent_Identity=35.7512953367876, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=135, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_PSEU5 (A4VQX9)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001174222.1
- ProteinModelPortal:   A4VQX9
- STRING:   A4VQX9
- GeneID:   5096718
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3757
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   GATCHAQ
- PhylomeDB:   A4VQX9
- ProtClustDB:   PRK00043
- BioCyc:   PSTU379731:PST_3757-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22683; Mature: 22683

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESSRLRGLYAITDSKLLADGRLLPYVEAALKGGARLLQYRDKTSDEARRLREADALQE
CCCCHHHCEEEEECCCCHHHCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHH
LCARHGAQLIINDDAELAARLGVGLHLGQEDGSLAAARALLGRQAIIGATCHAQLPLAEQ
HHHHCCCEEEECCCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AARDGASYVAFGRFFQSHTKPGAPAANHELLREARARIGLPIVAIGGITLDTAPSLIAEG
HHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCEECCCHHHHHHHH
VQMIAVIHALFAADSAAEVERRAQAFSQLFNTP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKESSRLRGLYAITDSKLLADGRLLPYVEAALKGGARLLQYRDKTSDEARRLREADALQE
CCCCHHHCEEEEECCCCHHHCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHH
LCARHGAQLIINDDAELAARLGVGLHLGQEDGSLAAARALLGRQAIIGATCHAQLPLAEQ
HHHHCCCEEEECCCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AARDGASYVAFGRFFQSHTKPGAPAANHELLREARARIGLPIVAIGGITLDTAPSLIAEG
HHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCEECCCHHHHHHHH
VQMIAVIHALFAADSAAEVERRAQAFSQLFNTP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA