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The map label for this gene is thiD [H]

Identifier: 146284068

GI number: 146284068

Start: 4064707

End: 4065570

Strand: Direct

Name: thiD [H]

Synonym: PST_3756

Alternate gene names: 146284068

Gene position: 4064707-4065570 (Clockwise)

Preceding gene: 146284065

Following gene: 146284069

Centisome position: 88.99

GC content: 70.25

Gene sequence:

>864_bases
GTGGCGGTGGCGGATAGACGCACAACTGCCGCGTCCCGCCAGCGCGGTTTGGTGATAAGCTCGCACGCCATGAAAAACTT
CACTTCCCGTCCCGTCGTCCTCTGCCTGTCCGGCCACGATCCCAGTGGCGGCGCCGGCCTGCAGGCGGACATCGAAGCCC
TGCTGGCACAGGCCTGCCACGCTGCGCCGACCGTCACCGCGCTCACCGTGCAGGACACCGTCAACGTCTCCGACTTCCGC
GTGCTCGACCGCGAGTGGGTACTCGCCCAGGCCAACGCAGTGATCGCCGACCTCCCCATCGCCGCCGTCAAGCTGGGCAT
GCTCGGCTCGGTTGAGATGGTCGAGACGGTGCTCGAGATCATGGCCCGGCTGCCGGGCGTGCCGCTGGTCTGCGACCCGG
TGCTGCGCGCCGGCGGTGGCGGCGCGCTGGGCAAGGAGGATGTCGGCTACGCCATGCGCGAGCGGCTGTTCCCGGTTTCC
ACCATCGCCACGCCGAATCTGCCGGAAGCTCGGATCCTTGCCGAACTGCCGGAGGGCAGCGCCGACGAGTGTGCCGAGCG
CCTGCTGCCGCATATCCGCCACCTGCTGATCACCGGCGGCCATGGCGACGAGAGCGAAGTGCACAACCGCCTGTATTGCC
GCGACGGCAGTCGTCACGACTTCACTTGCGCGCGCCTGCCGGGCAGCTATCACGGCTCCGGCTGCACGCTTGCCAGCGCC
CTGGCCGGTCGTCTGGCACTGGGCGAGGAGCTGACCAGCGCGGTCCGCTCGGCGCTCGACTACACCTGGCGCACGCTGCG
CGATGCCGAGGCCCCCGGCCACGGCCAGTACGTGCCGCGCCGCCTGCCCCTGGACCTGGCCTGA

Upstream 100 bases:

>100_bases
AAACCAAAGTGCCAGCAATCTACCACAGCGGCGTGTGCACCCGGAAAAAGCCGATTCGCCAGGATGACTTGGCCGATGAC
CGGTGCCCGCGGCGGTGGCG

Downstream 100 bases:

>100_bases
CTGGAGACCGGCGCATGAAGGAATCGTCCCGCCTGCGCGGCCTGTACGCCATCACCGACAGCAAATTGCTGGCCGATGGC
CGCCTGCTGCCCTATGTCGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAVADRRTTAASRQRGLVISSHAMKNFTSRPVVLCLSGHDPSGGAGLQADIEALLAQACHAAPTVTALTVQDTVNVSDFR
VLDREWVLAQANAVIADLPIAAVKLGMLGSVEMVETVLEIMARLPGVPLVCDPVLRAGGGGALGKEDVGYAMRERLFPVS
TIATPNLPEARILAELPEGSADECAERLLPHIRHLLITGGHGDESEVHNRLYCRDGSRHDFTCARLPGSYHGSGCTLASA
LAGRLALGEELTSAVRSALDYTWRTLRDAEAPGHGQYVPRRLPLDLA

Sequences:

>Translated_287_residues
MAVADRRTTAASRQRGLVISSHAMKNFTSRPVVLCLSGHDPSGGAGLQADIEALLAQACHAAPTVTALTVQDTVNVSDFR
VLDREWVLAQANAVIADLPIAAVKLGMLGSVEMVETVLEIMARLPGVPLVCDPVLRAGGGGALGKEDVGYAMRERLFPVS
TIATPNLPEARILAELPEGSADECAERLLPHIRHLLITGGHGDESEVHNRLYCRDGSRHDFTCARLPGSYHGSGCTLASA
LAGRLALGEELTSAVRSALDYTWRTLRDAEAPGHGQYVPRRLPLDLA
>Mature_286_residues
AVADRRTTAASRQRGLVISSHAMKNFTSRPVVLCLSGHDPSGGAGLQADIEALLAQACHAAPTVTALTVQDTVNVSDFRV
LDREWVLAQANAVIADLPIAAVKLGMLGSVEMVETVLEIMARLPGVPLVCDPVLRAGGGGALGKEDVGYAMRERLFPVST
IATPNLPEARILAELPEGSADECAERLLPHIRHLLITGGHGDESEVHNRLYCRDGSRHDFTCARLPGSYHGSGCTLASAL
AGRLALGEELTSAVRSALDYTWRTLRDAEAPGHGQYVPRRLPLDLA

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=218, Percent_Identity=34.8623853211009, Blast_Score=120, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6325378, Length=251, Percent_Identity=33.0677290836653, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324517, Length=250, Percent_Identity=33.2, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324997, Length=252, Percent_Identity=34.1269841269841, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30506; Mature: 30375

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVADRRTTAASRQRGLVISSHAMKNFTSRPVVLCLSGHDPSGGAGLQADIEALLAQACH
CCCCCCHHHHHHHHCCEEEEHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHC
AAPTVTALTVQDTVNVSDFRVLDREWVLAQANAVIADLPIAAVKLGMLGSVEMVETVLEI
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCHHHHHHHHHH
MARLPGVPLVCDPVLRAGGGGALGKEDVGYAMRERLFPVSTIATPNLPEARILAELPEGS
HHHCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCCCC
ADECAERLLPHIRHLLITGGHGDESEVHNRLYCRDGSRHDFTCARLPGSYHGSGCTLASA
HHHHHHHHHHHHHHHEEECCCCCHHHHHCEEEECCCCCCCEEEEECCCCCCCCCHHHHHH
LAGRLALGEELTSAVRSALDYTWRTLRDAEAPGHGQYVPRRLPLDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVADRRTTAASRQRGLVISSHAMKNFTSRPVVLCLSGHDPSGGAGLQADIEALLAQACH
CCCCCHHHHHHHHCCEEEEHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHC
AAPTVTALTVQDTVNVSDFRVLDREWVLAQANAVIADLPIAAVKLGMLGSVEMVETVLEI
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCHHHHHHHHHH
MARLPGVPLVCDPVLRAGGGGALGKEDVGYAMRERLFPVSTIATPNLPEARILAELPEGS
HHHCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCCCC
ADECAERLLPHIRHLLITGGHGDESEVHNRLYCRDGSRHDFTCARLPGSYHGSGCTLASA
HHHHHHHHHHHHHHHEEECCCCCHHHHHCEEEECCCCCCCEEEEECCCCCCCCCHHHHHH
LAGRLALGEELTSAVRSALDYTWRTLRDAEAPGHGQYVPRRLPLDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]