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The map label for this gene is pilR [H]

Identifier: 146283960

GI number: 146283960

Start: 3935421

End: 3936812

Strand: Direct

Name: pilR [H]

Synonym: PST_3646

Alternate gene names: 146283960

Gene position: 3935421-3936812 (Clockwise)

Preceding gene: 146283959

Following gene: 146283962

Centisome position: 86.16

GC content: 65.23

Gene sequence:

>1392_bases
ATGCTCGCCAGCCGCCCGGAAGGCGACACCCGCTTCAGGAACCAGAGCAAGACCATGACCGCGCGCCAGAAAGCCCTGAT
CATCGACGACGAGCCGGACATCCGCGAGCTGCTGGAGATCACCCTCGGCCGCATGAAGCTCGATACCCGCAGCGCACGCA
ACCTAAAGGAGGCGCGCGAGCTGCTGGCACGCGAGCACTACGACCTGTGCCTGACCGATATGCGCCTGCCCGACGGCAGC
GGCCTTGAGCTGGTCCAGCACATCCAGCAACAGTACCCGCAACTGCCCGTGGCGATGATCACCGCCTATGGCAGCCTGGA
TACCGCCATCGGCGCACTCAAGGCCGGTGCGTTCGACTTCCTCACCAAACCGGTGGACCTGAACCGCCTGCGCGAGCTGG
TCAGCACCGCCCTGCGCCTGCGCGCACCCGCAGCCGAAGCGCCGGTGGACAGTCGCCTGCTGGGCAGCTCGCCGCCGATG
AAAACCTTGCGCAAGCAGATCGGCAAGCTCGCCCGCAGCCAGGCACCCGTCTACATCAGCGGCGAGTCGGGCAGCGGCAA
GGAACTGGTGGCACGCCTGATTCATGAGCAGGGCCCGCGCAGCGAAAAGCCATTCGTGCCGGTCAATTGCGGTGCCATTC
CGTCGGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAGGGCAGCTTCAGCGGCGCCATCGAGGACAAGCCCGGTCTG
TTCCAGGCCGCCAATGGTGGCACGCTGTTTCTCGACGAGGTGGCGGACCTGCCGCTGCCGATGCAGGTCAAGCTGCTGCG
CGCCATCCAGGAAAAGGCCGTGCGTGCCGTTGGCGGTGCGCAGGAAGTGATGGTCGATGTACGCATCCTCTGCGCCACCC
ACAAGGATCTCGCCAGCGAGGTCGCCGCAGGCCGTTTTCGCCAGGATCTCTACTACCGCCTGAACGTCATCGAACTGCGT
GTACCGCCACTGCGCGAACGCCGCGAAGACATCGGCCTGCTCGCCGATGTCATGCTGCGCCGTCTGGCGCAGGAATGCGG
CGAGGCCACGGCCCGCCTGCATCCCGATGCCCTGGCCAAGCTGGAGAACTATCGCTTTCCGGGCAACGTCCGCGAACTGG
AGAACATGCTCGAACGCGCCTACACCCTGTGCGAAGGCGATGAGATCAAGGCTGGCGACCTGCGCCTGGCGGATAGCCCA
GGCCTGCCGGAAAACGGCGAAGCCAGCCTGGCGCAGATCGACAACCTGGAGGACCACCTGGAGGAGATCGAGCGCAAGCT
GATCATGCAGGCCCTCGAAGAAACACGCTGGAACCGCACCGCGGCCGCCCAGCGACTGGGGCTGACCTTCCGCTCGATGC
GTTACCGTCTCAAGAAACTCGGACTGGACTGA

Upstream 100 bases:

>100_bases
GCGCACCCGCGCAAGCTGAGCTAGGCGCGGCAGAACAGTTGCCGGCAACGCATCACACATCGACCAAAGCGGCAGCCACG
GCGGCGCCGGGAGAGTTAAC

Downstream 100 bases:

>100_bases
GTGGCAGGCGTAGACGCCTGCCACCGGATACGAACGCTGCGCCCTACCCCTGCGACAGCAGGTTGCGCAGATCGATGATC
GCCGCGTTGGCGCGGGAGAT

Product: two-component response regulator PilR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLASRPEGDTRFRNQSKTMTARQKALIIDDEPDIRELLEITLGRMKLDTRSARNLKEARELLAREHYDLCLTDMRLPDGS
GLELVQHIQQQYPQLPVAMITAYGSLDTAIGALKAGAFDFLTKPVDLNRLRELVSTALRLRAPAAEAPVDSRLLGSSPPM
KTLRKQIGKLARSQAPVYISGESGSGKELVARLIHEQGPRSEKPFVPVNCGAIPSELMESEFFGHKKGSFSGAIEDKPGL
FQAANGGTLFLDEVADLPLPMQVKLLRAIQEKAVRAVGGAQEVMVDVRILCATHKDLASEVAAGRFRQDLYYRLNVIELR
VPPLRERREDIGLLADVMLRRLAQECGEATARLHPDALAKLENYRFPGNVRELENMLERAYTLCEGDEIKAGDLRLADSP
GLPENGEASLAQIDNLEDHLEEIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGLD

Sequences:

>Translated_463_residues
MLASRPEGDTRFRNQSKTMTARQKALIIDDEPDIRELLEITLGRMKLDTRSARNLKEARELLAREHYDLCLTDMRLPDGS
GLELVQHIQQQYPQLPVAMITAYGSLDTAIGALKAGAFDFLTKPVDLNRLRELVSTALRLRAPAAEAPVDSRLLGSSPPM
KTLRKQIGKLARSQAPVYISGESGSGKELVARLIHEQGPRSEKPFVPVNCGAIPSELMESEFFGHKKGSFSGAIEDKPGL
FQAANGGTLFLDEVADLPLPMQVKLLRAIQEKAVRAVGGAQEVMVDVRILCATHKDLASEVAAGRFRQDLYYRLNVIELR
VPPLRERREDIGLLADVMLRRLAQECGEATARLHPDALAKLENYRFPGNVRELENMLERAYTLCEGDEIKAGDLRLADSP
GLPENGEASLAQIDNLEDHLEEIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGLD
>Mature_463_residues
MLASRPEGDTRFRNQSKTMTARQKALIIDDEPDIRELLEITLGRMKLDTRSARNLKEARELLAREHYDLCLTDMRLPDGS
GLELVQHIQQQYPQLPVAMITAYGSLDTAIGALKAGAFDFLTKPVDLNRLRELVSTALRLRAPAAEAPVDSRLLGSSPPM
KTLRKQIGKLARSQAPVYISGESGSGKELVARLIHEQGPRSEKPFVPVNCGAIPSELMESEFFGHKKGSFSGAIEDKPGL
FQAANGGTLFLDEVADLPLPMQVKLLRAIQEKAVRAVGGAQEVMVDVRILCATHKDLASEVAAGRFRQDLYYRLNVIELR
VPPLRERREDIGLLADVMLRRLAQECGEATARLHPDALAKLENYRFPGNVRELENMLERAYTLCEGDEIKAGDLRLADSP
GLPENGEASLAQIDNLEDHLEEIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGLD

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=40.9190371991247, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1790437, Length=440, Percent_Identity=43.8636363636364, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=39.1120507399577, Blast_Score=320, Evalue=8e-89,
Organism=Escherichia coli, GI1788905, Length=435, Percent_Identity=40, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=42.9467084639498, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789233, Length=334, Percent_Identity=41.3173652694611, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=43.0340557275542, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=321, Percent_Identity=42.6791277258567, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1787583, Length=338, Percent_Identity=38.1656804733728, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=42.7244582043344, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=37.8461538461538, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=36.7768595041322, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI87081858, Length=354, Percent_Identity=28.2485875706215, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787487, Length=117, Percent_Identity=37.6068376068376, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=32.258064516129, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=125, Percent_Identity=29.6, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51635; Mature: 51635

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASRPEGDTRFRNQSKTMTARQKALIIDDEPDIRELLEITLGRMKLDTRSARNLKEARE
CCCCCCCCCHHHHCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLAREHYDLCLTDMRLPDGSGLELVQHIQQQYPQLPVAMITAYGSLDTAIGALKAGAFDF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHCCHHHH
LTKPVDLNRLRELVSTALRLRAPAAEAPVDSRLLGSSPPMKTLRKQIGKLARSQAPVYIS
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
GESGSGKELVARLIHEQGPRSEKPFVPVNCGAIPSELMESEFFGHKKGSFSGAIEDKPGL
CCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
FQAANGGTLFLDEVADLPLPMQVKLLRAIQEKAVRAVGGAQEVMVDVRILCATHKDLASE
EEECCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VAAGRFRQDLYYRLNVIELRVPPLRERREDIGLLADVMLRRLAQECGEATARLHPDALAK
HHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LENYRFPGNVRELENMLERAYTLCEGDEIKAGDLRLADSPGLPENGEASLAQIDNLEDHL
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHCCHHHHH
EEIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGLD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLASRPEGDTRFRNQSKTMTARQKALIIDDEPDIRELLEITLGRMKLDTRSARNLKEARE
CCCCCCCCCHHHHCCHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLAREHYDLCLTDMRLPDGSGLELVQHIQQQYPQLPVAMITAYGSLDTAIGALKAGAFDF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHCCHHHH
LTKPVDLNRLRELVSTALRLRAPAAEAPVDSRLLGSSPPMKTLRKQIGKLARSQAPVYIS
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
GESGSGKELVARLIHEQGPRSEKPFVPVNCGAIPSELMESEFFGHKKGSFSGAIEDKPGL
CCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
FQAANGGTLFLDEVADLPLPMQVKLLRAIQEKAVRAVGGAQEVMVDVRILCATHKDLASE
EEECCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VAAGRFRQDLYYRLNVIELRVPPLRERREDIGLLADVMLRRLAQECGEATARLHPDALAK
HHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LENYRFPGNVRELENMLERAYTLCEGDEIKAGDLRLADSPGLPENGEASLAQIDNLEDHL
HHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHCCHHHHH
EEIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGLD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]