Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 146283962

GI number: 146283962

Start: 3938132

End: 3939727

Strand: Direct

Name: pleD [H]

Synonym: PST_3648

Alternate gene names: 146283962

Gene position: 3938132-3939727 (Clockwise)

Preceding gene: 146283960

Following gene: 146283963

Centisome position: 86.22

GC content: 64.22

Gene sequence:

>1596_bases
ATGTCGGATCAGGAAGAGCTTAAACAGCATTTCGCCCAGCGGGTCATCCACCAGGCACGGGAAGTGCTGGAGGTCTGGCA
GCAACTGCAACGCAGTGAATGGAACGAGGACTACCATGCCGCGTTGACCGAAGCGACGACTCGTCTTCGGCGCTTTGCCG
ATCGCTTCGAGCAGACCGAGCACAGCGAGCTGGCGTTGAACATCGAGGATTGCCTGAGCGACATCCGCGACAACCGCGGG
CGTCTGTCCAGCAGCACCATCTCCACACTGAGTCAGCTGATCTATCGCCTTGCCCGCACCGGACTGCGCCACAGCGATCC
GCACAACCAGTCGTTCCTGCCGCCGCTGAGCAAGCCGGTGTACATCGCCCTGCAAGACCAGGAGCGCGCCGAGCATCTGG
TACGCCAGTTGGACTTCTTCAGCATGTCCGCACAGGCTTTCAGCAATGTCGCCGCGTTCCGCACGGCGATGCATCGCCGG
CACCCGGCCGCCATCGTGATGGAGGTGGACTTCAACGGACCGGGCGAAGGGCTGCGCCTCGCCGAACAGGTGCAACTCGG
TCTGGATCCGAAGCTGCCCATGCTGTTCTTCAGCCACGCCGAAACCGACACGCCGACTCGCCTGGCCGCCGTCCGCGCCG
GCGGTCTGGAGTTCTTCACCGGCACCCTGGACGCCAGCAGCCTGCTGGAGAAGATCGAGATCTTCACCCGCACCGCGCAT
TACGAGCCGATTCGCGTGCTGATCATCGATGACTCGCGTGCCCAGGCGACGCATACCGAGCGGGTGCTGAACAACGCCGG
CATCGTCACCCAGACCCTGACCGAGCCGATCCAGGCCATCGGCGCACTCGCCGAGTTCCAGCCGGACCTGATCATCCTCG
ACATGTACATGCCCGACTGCCAGGGCACCGAGCTGGCCAAGGTAATCCGCCAGCATGAGCGCTACGTCAGCGTGCCGATC
ATCTACCTGTCCGCCGAGGACGACCTGGACAAGCAGTTGGACGCCATGGGCGAAGGCGGCGACGACTTTCTCACCAAGCC
GATCAAGCCGAGCCACCTGATCGCCACGGTACGCACCCGCGCCACCCGCGCGCGCAGCCTGAAGGCGCGCATCGTGCGCG
ACAGCCTCACCGGTCTGTACAACCACACCCACAGCCTGCAGCTACTCGAGGATGCTCGCTTCCGCGCCCGTCGCGACAAT
CGACCGCTGAGCTTCGCCATGCTCGACATCGATCACTTCAAGCAGGTCAACGACACCTTCGGCCACCCCATGGGTGACCG
AGTCATCAAGAGCCTGGCGCTGTTTCTCAAGCAGCGCCTGCGCAAGACCGATCACATCGGCCGCTACGGTGGCGAGGAGT
TCGCCGTGGTGCTGCCAGACACCCCGGCAGAGGCCGCCTGCAAGGTGCTGAACGAGATCCGCCAGCGTTTCGCCGAGATC
CGTTTCCCGGCGCAGCCGCGCGATCTGAGCTGCACCTTCAGCTGCGGCATCACCGAGCTGACCGCCGATGCCGAGGTCAA
GACGCTTACCCAGCAGGCCGACGAGGCGCTCTATCGTGCCAAGCACGCCGGTCGTAACCGCGTCGAGCGTTACTAG

Upstream 100 bases:

>100_bases
CACTACCGCGCAACGCCCTTCCAAAGCGCACCGACGGTGATAGCATGCCGCCATTACAATGCACCAGGACAGCGCACCTG
GCGCGCCAGGAATAATCGCG

Downstream 100 bases:

>100_bases
CCGCCAATACAGGCGCGGCCGCCAACGTCGAGGGTCGTGTAGCGATCGTCGGAGCGGCCGGCTGTCACGCGAATGACATC
AAACTGCCATACCCTGCAAG

Product: two-component response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSDQEELKQHFAQRVIHQAREVLEVWQQLQRSEWNEDYHAALTEATTRLRRFADRFEQTEHSELALNIEDCLSDIRDNRG
RLSSSTISTLSQLIYRLARTGLRHSDPHNQSFLPPLSKPVYIALQDQERAEHLVRQLDFFSMSAQAFSNVAAFRTAMHRR
HPAAIVMEVDFNGPGEGLRLAEQVQLGLDPKLPMLFFSHAETDTPTRLAAVRAGGLEFFTGTLDASSLLEKIEIFTRTAH
YEPIRVLIIDDSRAQATHTERVLNNAGIVTQTLTEPIQAIGALAEFQPDLIILDMYMPDCQGTELAKVIRQHERYVSVPI
IYLSAEDDLDKQLDAMGEGGDDFLTKPIKPSHLIATVRTRATRARSLKARIVRDSLTGLYNHTHSLQLLEDARFRARRDN
RPLSFAMLDIDHFKQVNDTFGHPMGDRVIKSLALFLKQRLRKTDHIGRYGGEEFAVVLPDTPAEAACKVLNEIRQRFAEI
RFPAQPRDLSCTFSCGITELTADAEVKTLTQQADEALYRAKHAGRNRVERY

Sequences:

>Translated_531_residues
MSDQEELKQHFAQRVIHQAREVLEVWQQLQRSEWNEDYHAALTEATTRLRRFADRFEQTEHSELALNIEDCLSDIRDNRG
RLSSSTISTLSQLIYRLARTGLRHSDPHNQSFLPPLSKPVYIALQDQERAEHLVRQLDFFSMSAQAFSNVAAFRTAMHRR
HPAAIVMEVDFNGPGEGLRLAEQVQLGLDPKLPMLFFSHAETDTPTRLAAVRAGGLEFFTGTLDASSLLEKIEIFTRTAH
YEPIRVLIIDDSRAQATHTERVLNNAGIVTQTLTEPIQAIGALAEFQPDLIILDMYMPDCQGTELAKVIRQHERYVSVPI
IYLSAEDDLDKQLDAMGEGGDDFLTKPIKPSHLIATVRTRATRARSLKARIVRDSLTGLYNHTHSLQLLEDARFRARRDN
RPLSFAMLDIDHFKQVNDTFGHPMGDRVIKSLALFLKQRLRKTDHIGRYGGEEFAVVLPDTPAEAACKVLNEIRQRFAEI
RFPAQPRDLSCTFSCGITELTADAEVKTLTQQADEALYRAKHAGRNRVERY
>Mature_530_residues
SDQEELKQHFAQRVIHQAREVLEVWQQLQRSEWNEDYHAALTEATTRLRRFADRFEQTEHSELALNIEDCLSDIRDNRGR
LSSSTISTLSQLIYRLARTGLRHSDPHNQSFLPPLSKPVYIALQDQERAEHLVRQLDFFSMSAQAFSNVAAFRTAMHRRH
PAAIVMEVDFNGPGEGLRLAEQVQLGLDPKLPMLFFSHAETDTPTRLAAVRAGGLEFFTGTLDASSLLEKIEIFTRTAHY
EPIRVLIIDDSRAQATHTERVLNNAGIVTQTLTEPIQAIGALAEFQPDLIILDMYMPDCQGTELAKVIRQHERYVSVPII
YLSAEDDLDKQLDAMGEGGDDFLTKPIKPSHLIATVRTRATRARSLKARIVRDSLTGLYNHTHSLQLLEDARFRARRDNR
PLSFAMLDIDHFKQVNDTFGHPMGDRVIKSLALFLKQRLRKTDHIGRYGGEEFAVVLPDTPAEAACKVLNEIRQRFAEIR
FPAQPRDLSCTFSCGITELTADAEVKTLTQQADEALYRAKHAGRNRVERY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=39.4117647058824, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=33.9622641509434, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=155, Percent_Identity=36.1290322580645, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=39.6449704142012, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=35, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787816, Length=201, Percent_Identity=29.3532338308458, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=30.6451612903226, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081974, Length=175, Percent_Identity=30.2857142857143, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788381, Length=129, Percent_Identity=34.1085271317829, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=30.5732484076433, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787056, Length=124, Percent_Identity=33.0645161290323, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=112, Percent_Identity=33.0357142857143, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788956, Length=176, Percent_Identity=31.25, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=26.875, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 60389; Mature: 60258

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQEELKQHFAQRVIHQAREVLEVWQQLQRSEWNEDYHAALTEATTRLRRFADRFEQTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
HSELALNIEDCLSDIRDNRGRLSSSTISTLSQLIYRLARTGLRHSDPHNQSFLPPLSKPV
CHHEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
YIALQDQERAEHLVRQLDFFSMSAQAFSNVAAFRTAMHRRHPAAIVMEVDFNGPGEGLRL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHH
AEQVQLGLDPKLPMLFFSHAETDTPTRLAAVRAGGLEFFTGTLDASSLLEKIEIFTRTAH
HHHHHHCCCCCCCHHEEECCCCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCC
YEPIRVLIIDDSRAQATHTERVLNNAGIVTQTLTEPIQAIGALAEFQPDLIILDMYMPDC
CCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
QGTELAKVIRQHERYVSVPIIYLSAEDDLDKQLDAMGEGGDDFLTKPIKPSHLIATVRTR
CCHHHHHHHHHHHCEEEEEEEEEECCCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHH
ATRARSLKARIVRDSLTGLYNHTHSLQLLEDARFRARRDNRPLSFAMLDIDHFKQVNDTF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
GHPMGDRVIKSLALFLKQRLRKTDHIGRYGGEEFAVVLPDTPAEAACKVLNEIRQRFAEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHC
RFPAQPRDLSCTFSCGITELTADAEVKTLTQQADEALYRAKHAGRNRVERY
CCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
SDQEELKQHFAQRVIHQAREVLEVWQQLQRSEWNEDYHAALTEATTRLRRFADRFEQTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
HSELALNIEDCLSDIRDNRGRLSSSTISTLSQLIYRLARTGLRHSDPHNQSFLPPLSKPV
CHHEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
YIALQDQERAEHLVRQLDFFSMSAQAFSNVAAFRTAMHRRHPAAIVMEVDFNGPGEGLRL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHH
AEQVQLGLDPKLPMLFFSHAETDTPTRLAAVRAGGLEFFTGTLDASSLLEKIEIFTRTAH
HHHHHHCCCCCCCHHEEECCCCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCC
YEPIRVLIIDDSRAQATHTERVLNNAGIVTQTLTEPIQAIGALAEFQPDLIILDMYMPDC
CCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
QGTELAKVIRQHERYVSVPIIYLSAEDDLDKQLDAMGEGGDDFLTKPIKPSHLIATVRTR
CCHHHHHHHHHHHCEEEEEEEEEECCCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHH
ATRARSLKARIVRDSLTGLYNHTHSLQLLEDARFRARRDNRPLSFAMLDIDHFKQVNDTF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
GHPMGDRVIKSLALFLKQRLRKTDHIGRYGGEEFAVVLPDTPAEAACKVLNEIRQRFAEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHC
RFPAQPRDLSCTFSCGITELTADAEVKTLTQQADEALYRAKHAGRNRVERY
CCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA