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The map label for this gene is rutA

Identifier: 146283914

GI number: 146283914

Start: 3885598

End: 3886680

Strand: Direct

Name: rutA

Synonym: PST_3597

Alternate gene names: 146283914

Gene position: 3885598-3886680 (Clockwise)

Preceding gene: 146283911

Following gene: 146283915

Centisome position: 85.07

GC content: 64.73

Gene sequence:

>1083_bases
ATGGATGTTGGTGTTTTCATTCCGATCGGCAACAACGGCTGGCTCATCTCCGAGAATGCGCCGCAGTACATGCCGACCTT
CGAACTGAACAAAGCCATCGTGCAGAAGGCCGAGGGCTACGGATTCGACTTCGCGCTGTCGATGATCAAGCTGCGCGGCT
TCGGTGGTAAGACCGAGTTCTGGGAGCACAACCTGGAATCCTTCACGCTGATGGCGGGCCTGGCCGCGGTCACCAGCAAG
ATCCAGCTGTTCGCGACTGCCGCAACGCTCACTCTGCCGCCGGCCATCGTCGCGCGCATGGCCTCGACCATCGACTCGAT
CTCCGGCGGCCGCTTCGGCGTGAACCTCGTCACCGGCTGGCAAAAGCCGGAGTACAGCCAGATGGGCCTGTGGCCGGGGG
ATGAATTCTTCGGCACCCGTTATCAGTATCTCGGCGAGTACGCCCAGGTGCTGCGCGACCTCTGGGCCACCGGCCGCAGC
GATTTCAAGGGCGAGCACTTCCAGATGGAGGATTGCCGCGTCAGCCCGAAACCCCAGGCGGACATGAAGATCATCTGCGC
CGGCTCGTCCGATGCCGGCATGGCCTTCTCGGCGCAGTACGCCGACTACAACTTCTGCTTCGGCAAGGGCGTGAACACCC
CGACCGCCTTCGCCCCGGCCGCCGAACGGCTGCAGGCGGCCTGTGCCAAGACCGGCCGCCACGTCACCTCCTGCGTGCTG
TTCATGGTGATCGCCGACGAGACCGACGAAGCGGCCCGGGCCAAGTGGGAGCACTACAAGGCCGGCGCCGACGAGGAAGC
CATCGCCTGGCTCGGCGAGCAGGGTGCCGCCGACAAGGGTGCGGACAGCAACATCCGGCAGATGGCCGACCCGACCTCGG
CGGTGAACATCAACATGGGCACGCTGGTGGGCTCCTACGCCAACGTAGCGCGGATGCTCGACGAAATCGCCACGGTGCCG
GGCATGCAGGGCGTGATGCTGACCTTCGATGACTTTCTCGAGGGCGTCGAAGCCTTCGGCCAGAAGATCCAGCCGCTGAT
GCAGAGCCGCCGGCACGTCACCGCGCTGAAGGAATCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGAATCCGTTTCTGGCCTACATCCTGCTAAAGCCCCTGCGACCCTGCCCGTCACGTTCGGGCAGCGCTTTCTAGCCAT
TGCGTACAGAGGTGCTACCC

Downstream 100 bases:

>100_bases
TGAACGCCGTCGAACAGATCTCCGGCTACGGCCTGAGCGCAACGGACGAACCTGTGCAACTGCCGGCACGACCAGAGCCG
CTGGCGATGAAGGCGAGCGA

Product: luciferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDVGVFIPIGNNGWLISENAPQYMPTFELNKAIVQKAEGYGFDFALSMIKLRGFGGKTEFWEHNLESFTLMAGLAAVTSK
IQLFATAATLTLPPAIVARMASTIDSISGGRFGVNLVTGWQKPEYSQMGLWPGDEFFGTRYQYLGEYAQVLRDLWATGRS
DFKGEHFQMEDCRVSPKPQADMKIICAGSSDAGMAFSAQYADYNFCFGKGVNTPTAFAPAAERLQAACAKTGRHVTSCVL
FMVIADETDEAARAKWEHYKAGADEEAIAWLGEQGAADKGADSNIRQMADPTSAVNINMGTLVGSYANVARMLDEIATVP
GMQGVMLTFDDFLEGVEAFGQKIQPLMQSRRHVTALKESA

Sequences:

>Translated_360_residues
MDVGVFIPIGNNGWLISENAPQYMPTFELNKAIVQKAEGYGFDFALSMIKLRGFGGKTEFWEHNLESFTLMAGLAAVTSK
IQLFATAATLTLPPAIVARMASTIDSISGGRFGVNLVTGWQKPEYSQMGLWPGDEFFGTRYQYLGEYAQVLRDLWATGRS
DFKGEHFQMEDCRVSPKPQADMKIICAGSSDAGMAFSAQYADYNFCFGKGVNTPTAFAPAAERLQAACAKTGRHVTSCVL
FMVIADETDEAARAKWEHYKAGADEEAIAWLGEQGAADKGADSNIRQMADPTSAVNINMGTLVGSYANVARMLDEIATVP
GMQGVMLTFDDFLEGVEAFGQKIQPLMQSRRHVTALKESA
>Mature_360_residues
MDVGVFIPIGNNGWLISENAPQYMPTFELNKAIVQKAEGYGFDFALSMIKLRGFGGKTEFWEHNLESFTLMAGLAAVTSK
IQLFATAATLTLPPAIVARMASTIDSISGGRFGVNLVTGWQKPEYSQMGLWPGDEFFGTRYQYLGEYAQVLRDLWATGRS
DFKGEHFQMEDCRVSPKPQADMKIICAGSSDAGMAFSAQYADYNFCFGKGVNTPTAFAPAAERLQAACAKTGRHVTSCVL
FMVIADETDEAARAKWEHYKAGADEEAIAWLGEQGAADKGADSNIRQMADPTSAVNINMGTLVGSYANVARMLDEIATVP
GMQGVMLTFDDFLEGVEAFGQKIQPLMQSRRHVTALKESA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily

Homologues:

Organism=Escherichia coli, GI1787247, Length=360, Percent_Identity=78.8888888888889, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1787166, Length=307, Percent_Identity=25.4071661237785, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTA_PSEU5 (A4VQH4)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001174067.1
- ProteinModelPortal:   A4VQH4
- STRING:   A4VQH4
- GeneID:   5096558
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3597
- eggNOG:   COG2141
- HOGENOM:   HBG350183
- OMA:   FIPIGNN
- PhylomeDB:   A4VQH4
- ProtClustDB:   CLSK879925
- BioCyc:   PSTU379731:PST_3597-MONOMER
- HAMAP:   MF_01699
- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048
- Gene3D:   G3DSA:3.20.20.30
- TIGRFAMs:   TIGR03612

Pfam domain/function: PF00296 Bac_luciferase; SSF51679 Luciferase_like

EC number: NA

Molecular weight: Translated: 39197; Mature: 39197

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: BINDING 115-115 BINDING 124-124 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVGVFIPIGNNGWLISENAPQYMPTFELNKAIVQKAEGYGFDFALSMIKLRGFGGKTEF
CCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHH
WEHNLESFTLMAGLAAVTSKIQLFATAATLTLPPAIVARMASTIDSISGGRFGVNLVTGW
HHCCHHHHHHHHHHHHHHHHHHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECC
QKPEYSQMGLWPGDEFFGTRYQYLGEYAQVLRDLWATGRSDFKGEHFQMEDCRVSPKPQA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCC
DMKIICAGSSDAGMAFSAQYADYNFCFGKGVNTPTAFAPAAERLQAACAKTGRHVTSCVL
CEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
FMVIADETDEAARAKWEHYKAGADEEAIAWLGEQGAADKGADSNIRQMADPTSAVNINMG
HHHEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEEHH
TLVGSYANVARMLDEIATVPGMQGVMLTFDDFLEGVEAFGQKIQPLMQSRRHVTALKESA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVGVFIPIGNNGWLISENAPQYMPTFELNKAIVQKAEGYGFDFALSMIKLRGFGGKTEF
CCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHH
WEHNLESFTLMAGLAAVTSKIQLFATAATLTLPPAIVARMASTIDSISGGRFGVNLVTGW
HHCCHHHHHHHHHHHHHHHHHHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECC
QKPEYSQMGLWPGDEFFGTRYQYLGEYAQVLRDLWATGRSDFKGEHFQMEDCRVSPKPQA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCC
DMKIICAGSSDAGMAFSAQYADYNFCFGKGVNTPTAFAPAAERLQAACAKTGRHVTSCVL
CEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
FMVIADETDEAARAKWEHYKAGADEEAIAWLGEQGAADKGADSNIRQMADPTSAVNINMG
HHHEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEEHH
TLVGSYANVARMLDEIATVPGMQGVMLTFDDFLEGVEAFGQKIQPLMQSRRHVTALKESA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA