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The map label for this gene is rutB

Identifier: 146283915

GI number: 146283915

Start: 3886680

End: 3887426

Strand: Direct

Name: rutB

Synonym: PST_3598

Alternate gene names: 146283915

Gene position: 3886680-3887426 (Clockwise)

Preceding gene: 146283914

Following gene: 146283916

Centisome position: 85.1

GC content: 65.6

Gene sequence:

>747_bases
ATGAACGCCGTCGAACAGATCTCCGGCTACGGCCTGAGCGCAACGGACGAACCTGTGCAACTGCCGGCACGACCAGAGCC
GCTGGCGATGAAGGCGAGCGAAACCGCGCTAATCGTGGTGGATATGCAGAACGCCTATGCCAGCCGCGGCGGCTATCTCG
ACCTGGCAGGTTTCGATGTTTCGGCGACCCAGCCGGTGATCGCGAAGATCCGCCAGGCGCTGAACGCGGCCCGCGCCGCG
GGCATGCAGGTGATCTTTTTGCAGAACGGCTGGGACAACGGGTACGTCGAGGCGGGCGGGCCGGGCTCGCCGAACTGGCA
CAAGTCCAACGCGCTGAAGACCATGCGCAAGCGCCCGGAACTGGCCGGCACACTGTTGGCCAAGGGCGGTTGGGATTACG
CGCTGGTGGACGAACTGGCACCGCAGCCCGGCGACATCCTGGTGCCCAAGCCGCGCTACAGCGGTTTCTTCAATTCACAG
CTGGACAGCACCCTGCGCGCCCGTGGCATCCGCAACCTGGTGTTCACCGGGATCGCCACCAACGTGTGCGTGGAATCGAC
GCTGCGCGACGGCTTCCACCTGGAATATTTCGGTGTGGTGCTGGCCGATGCCACCCACCAGGCCGGGCCGGAATTCGCCC
AGCAGGCGGCGCTGTACAACATCGAAACGTTCTTCGGCTGGGTCTCCAGCGTCGACGCCTTCTGCCAGAGCTTCGCGGCC
GCGCCAGAGGTGGCGGTCGCCAGCTGA

Upstream 100 bases:

>100_bases
CCTTCGATGACTTTCTCGAGGGCGTCGAAGCCTTCGGCCAGAAGATCCAGCCGCTGATGCAGAGCCGCCGGCACGTCACC
GCGCTGAAGGAATCGGCCTG

Downstream 100 bases:

>100_bases
TTACCCCAAGTGCCCGACCGCGGCGCCCGCAGAGGCGCCCGCCGGGTTCGCCTGAATGCCTTGCAAGGAATCAACGACAT
GCCCAAGCAATCCATCATCC

Product: isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNAVEQISGYGLSATDEPVQLPARPEPLAMKASETALIVVDMQNAYASRGGYLDLAGFDVSATQPVIAKIRQALNAARAA
GMQVIFLQNGWDNGYVEAGGPGSPNWHKSNALKTMRKRPELAGTLLAKGGWDYALVDELAPQPGDILVPKPRYSGFFNSQ
LDSTLRARGIRNLVFTGIATNVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALYNIETFFGWVSSVDAFCQSFAA
APEVAVAS

Sequences:

>Translated_248_residues
MNAVEQISGYGLSATDEPVQLPARPEPLAMKASETALIVVDMQNAYASRGGYLDLAGFDVSATQPVIAKIRQALNAARAA
GMQVIFLQNGWDNGYVEAGGPGSPNWHKSNALKTMRKRPELAGTLLAKGGWDYALVDELAPQPGDILVPKPRYSGFFNSQ
LDSTLRARGIRNLVFTGIATNVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALYNIETFFGWVSSVDAFCQSFAA
APEVAVAS
>Mature_248_residues
MNAVEQISGYGLSATDEPVQLPARPEPLAMKASETALIVVDMQNAYASRGGYLDLAGFDVSATQPVIAKIRQALNAARAA
GMQVIFLQNGWDNGYVEAGGPGSPNWHKSNALKTMRKRPELAGTLLAKGGWDYALVDELAPQPGDILVPKPRYSGFFNSQ
LDSTLRARGIRNLVFTGIATNVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALYNIETFFGWVSSVDAFCQSFAA
APEVAVAS

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily

Homologues:

Organism=Escherichia coli, GI87081820, Length=219, Percent_Identity=76.2557077625571, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87081992, Length=184, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTB_PSEU5 (A4VQH5)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001174068.1
- ProteinModelPortal:   A4VQH5
- STRING:   A4VQH5
- GeneID:   5096559
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3598
- eggNOG:   COG1335
- HOGENOM:   HBG752434
- OMA:   MQNAYAS
- PhylomeDB:   A4VQH5
- ProtClustDB:   CLSK879924
- BioCyc:   PSTU379731:PST_3598-MONOMER
- HAMAP:   MF_00830
- InterPro:   IPR019916
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850
- TIGRFAMs:   TIGR03614

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: NA

Molecular weight: Translated: 26558; Mature: 26558

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVEQISGYGLSATDEPVQLPARPEPLAMKASETALIVVDMQNAYASRGGYLDLAGFDV
CCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHCCCCEEEEECCCC
SATQPVIAKIRQALNAARAAGMQVIFLQNGWDNGYVEAGGPGSPNWHKSNALKTMRKRPE
CCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCHH
LAGTLLAKGGWDYALVDELAPQPGDILVPKPRYSGFFNSQLDSTLRARGIRNLVFTGIAT
HHHHHHHCCCCCCHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALYNIETFFGWVSSVDAFCQSFAA
HHHHHHHHCCCCCEEEHHEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APEVAVAS
CCCCCCCC
>Mature Secondary Structure
MNAVEQISGYGLSATDEPVQLPARPEPLAMKASETALIVVDMQNAYASRGGYLDLAGFDV
CCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCHHHHCCCCEEEEECCCC
SATQPVIAKIRQALNAARAAGMQVIFLQNGWDNGYVEAGGPGSPNWHKSNALKTMRKRPE
CCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCHH
LAGTLLAKGGWDYALVDELAPQPGDILVPKPRYSGFFNSQLDSTLRARGIRNLVFTGIAT
HHHHHHHCCCCCCHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALYNIETFFGWVSSVDAFCQSFAA
HHHHHHHHCCCCCEEEHHEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APEVAVAS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA