Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 146283793

GI number: 146283793

Start: 3759445

End: 3760059

Strand: Direct

Name: rhtB [H]

Synonym: PST_3476

Alternate gene names: 146283793

Gene position: 3759445-3760059 (Clockwise)

Preceding gene: 146283786

Following gene: 146283794

Centisome position: 82.31

GC content: 63.74

Gene sequence:

>615_bases
ATGCCCAGCCTCGAATATCTGATTACCTGTCTGGTCGTCGTGCTGATACCTGGCGCGGGTGTGATCTACACGGTATCGAC
GGCACTGGTGCATGGGCGTCGCGCCGGCATCCTGGCCGCGCTGGCCTGTACGCTCGGGATCCTGCCGCATCTGTTGGCGA
CCATTCTCGGCGTCGCCGCCATCCTGCATTCCAGTGCGCTGGTTTTCGAGACGCTGCGCTATGCCGGTGCCGCGTACCTG
CTCTATCTCGCCTACGCAACCTGGCGCGATCGTTCCGCGCTGGCGGTGGGCGGCGATGGTGCGACACTCGACATTCGATC
GCTGGTTGCCAAGGCGCTGCTGGTCAATCTGCTGAACCCGAAGCTGACCATCTTCTTCCTGGCCTTCCTGCCGCAGTTCG
TCCGGCCCGGCGCCGGTGACGAGCTTGCGCAACTGCTCGGGCTCAGCGCGACCTTCATGCTGATGACCTTTGTCATCTTT
GCCATCTACGGCTTGCTGGCCCATGCTTTTCGCAAGGCGGTCGTCGAGTCGCAGCAGGTTCAGGCCTGGCTCCGTCGCGG
GTTTGCGGCGTCCTTTGCCGGACTCGGTGCAAACCTGGCGCTGGCCGACCGATGA

Upstream 100 bases:

>100_bases
TTCAACCCGCCATGCGCAGTTCGATCGGCGCCCGGCCCGGATAGTCTCGATGGATGCTCTATGCTGGTTCACTCCAACCG
TCCCATAGCAAGGAGTCCCC

Downstream 100 bases:

>100_bases
ACGCCCTGGGCGGCTGAAGCCGACCGAGTACAGCAGTCCGAAACCCTGGGCCAGCGCCTCGCTCGTCGGCGATGAGGCCG
GCCCGCGCTTCTTCTGCGTG

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPSLEYLITCLVVVLIPGAGVIYTVSTALVHGRRAGILAALACTLGILPHLLATILGVAAILHSSALVFETLRYAGAAYL
LYLAYATWRDRSALAVGGDGATLDIRSLVAKALLVNLLNPKLTIFFLAFLPQFVRPGAGDELAQLLGLSATFMLMTFVIF
AIYGLLAHAFRKAVVESQQVQAWLRRGFAASFAGLGANLALADR

Sequences:

>Translated_204_residues
MPSLEYLITCLVVVLIPGAGVIYTVSTALVHGRRAGILAALACTLGILPHLLATILGVAAILHSSALVFETLRYAGAAYL
LYLAYATWRDRSALAVGGDGATLDIRSLVAKALLVNLLNPKLTIFFLAFLPQFVRPGAGDELAQLLGLSATFMLMTFVIF
AIYGLLAHAFRKAVVESQQVQAWLRRGFAASFAGLGANLALADR
>Mature_203_residues
PSLEYLITCLVVVLIPGAGVIYTVSTALVHGRRAGILAALACTLGILPHLLATILGVAAILHSSALVFETLRYAGAAYLL
YLAYATWRDRSALAVGGDGATLDIRSLVAKALLVNLLNPKLTIFFLAFLPQFVRPGAGDELAQLLGLSATFMLMTFVIFA
IYGLLAHAFRKAVVESQQVQAWLRRGFAASFAGLGANLALADR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=197, Percent_Identity=32.48730964467, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788099, Length=156, Percent_Identity=28.8461538461538, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21658; Mature: 21527

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLEYLITCLVVVLIPGAGVIYTVSTALVHGRRAGILAALACTLGILPHLLATILGVAA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILHSSALVFETLRYAGAAYLLYLAYATWRDRSALAVGGDGATLDIRSLVAKALLVNLLNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCC
KLTIFFLAFLPQFVRPGAGDELAQLLGLSATFMLMTFVIFAIYGLLAHAFRKAVVESQQV
HHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAWLRRGFAASFAGLGANLALADR
HHHHHHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure 
PSLEYLITCLVVVLIPGAGVIYTVSTALVHGRRAGILAALACTLGILPHLLATILGVAA
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILHSSALVFETLRYAGAAYLLYLAYATWRDRSALAVGGDGATLDIRSLVAKALLVNLLNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCC
KLTIFFLAFLPQFVRPGAGDELAQLLGLSATFMLMTFVIFAIYGLLAHAFRKAVVESQQV
HHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAWLRRGFAASFAGLGANLALADR
HHHHHHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]