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The map label for this gene is IS1086 [H]

Identifier: 146283736

GI number: 146283736

Start: 3692464

End: 3693492

Strand: Direct

Name: IS1086 [H]

Synonym: PST_3419

Alternate gene names: 146283736

Gene position: 3692464-3693492 (Clockwise)

Preceding gene: 146283734

Following gene: 146283740

Centisome position: 80.84

GC content: 60.35

Gene sequence:

>1029_bases
ATGTCTTATACCGAACTCAGCGTTGAAGAGCGCGCCACCATTCAAATCGGTCGTACCCAAGGCTTCAGCCTGCGCAGGAT
TGCCTGCTTGATCAACCGATCCCCTTCGACCATCAGCCGTGAGCTGCGCCGTAACCGAGGTGCTTGCGGTGGCTACTCGG
CCCGCCTGGCCCAGCAGCAAATGCAGGCCCGCCGCCAGGTTTGTCGACCGATGCGAAAACTGTTGCCGGGTAGCGAGCGC
TTCGAACTGGTGACCCATATGCTGCGTGAGCGTTTGTCTCCCGAGCAGATTGCCGGCAAGCTGCGCAGCATGAACATACC
CAACCTGCGAGATGCCTACGTCTGTCGCGAGACGATCTACAACGCGATCTATGCCCTGCCAGTGGGTGAGCTGCGTAAGG
AGCTGATCATCTGTCTGCGCCAAGGCAAGACGACGCGCCGGCCGCGCTCTGGTGGCGTGGATCGGCGCGGCCAGATCCCC
GAGATGGTCAGTATTCATGTGCGCCCGCCGGAGATTGAAGACAGGCTGATGCCGGGGCATTGGGAAGGCGACCTCATCAA
GGGTAAGGCCAACGCCTCGTCCGTCGGTACGCTGGTGGAACGCACCAGTGGCTACCTGATGTTGGTGAAGATGAACGACG
CGACGGCGACTTCGGCGCTGGAAGGCTTCAGCGCCGCGCTCAATAGCATGCCGCTGGAGATGCGCAAGAGCATGACTTAC
GACCAGGGCCGGGAGATGGCGCGACACGCCGAGATCACCCAAAGAACCGGCGTGGCGATCTACTTCTGCGACCCGCACAG
CCCCTGGCAGCGCGGCAGCAACGAAAACATCAACGGCCTGATTCGCCAGTACTTGCCCAAGGGCACAGACCTGTCGGTAC
ATAGCCAGGAGGAGCTGGACGCCATTGCGCTGCAACTGAACATGCGACCGCGCAAGCGCTTCGACTTCAAATGCCCCATC
GAGGTGATGGGAGAGGTCATGCAAAATGCCATGGCAATGCGGCATGATGCTCCGGTTTCAATTCAATAA

Upstream 100 bases:

>100_bases
ATGCCGCAACGCGTTGGATAGCAGGTTGGAAATGGCCCGTTGGATGGCGGCGGCAAAAACTGAGTGCAACACCTGACCTT
TCCGGTACGGTGCTGCCCCT

Downstream 100 bases:

>100_bases
CCGTGTTGCACTCAGCTCCTGCAACCGCCGACCATAAGCCGATTTCCTAGAACTATGGCATCGCCCGTTACCGTCGTTGC
GACTCCGGCTTCTTCGGCGA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSYTELSVEERATIQIGRTQGFSLRRIACLINRSPSTISRELRRNRGACGGYSARLAQQQMQARRQVCRPMRKLLPGSER
FELVTHMLRERLSPEQIAGKLRSMNIPNLRDAYVCRETIYNAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIP
EMVSIHVRPPEIEDRLMPGHWEGDLIKGKANASSVGTLVERTSGYLMLVKMNDATATSALEGFSAALNSMPLEMRKSMTY
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELDAIALQLNMRPRKRFDFKCPI
EVMGEVMQNAMAMRHDAPVSIQ

Sequences:

>Translated_342_residues
MSYTELSVEERATIQIGRTQGFSLRRIACLINRSPSTISRELRRNRGACGGYSARLAQQQMQARRQVCRPMRKLLPGSER
FELVTHMLRERLSPEQIAGKLRSMNIPNLRDAYVCRETIYNAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIP
EMVSIHVRPPEIEDRLMPGHWEGDLIKGKANASSVGTLVERTSGYLMLVKMNDATATSALEGFSAALNSMPLEMRKSMTY
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELDAIALQLNMRPRKRFDFKCPI
EVMGEVMQNAMAMRHDAPVSIQ
>Mature_341_residues
SYTELSVEERATIQIGRTQGFSLRRIACLINRSPSTISRELRRNRGACGGYSARLAQQQMQARRQVCRPMRKLLPGSERF
ELVTHMLRERLSPEQIAGKLRSMNIPNLRDAYVCRETIYNAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIPE
MVSIHVRPPEIEDRLMPGHWEGDLIKGKANASSVGTLVERTSGYLMLVKMNDATATSALEGFSAALNSMPLEMRKSMTYD
QGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELDAIALQLNMRPRKRFDFKCPIE
VMGEVMQNAMAMRHDAPVSIQ

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=329, Percent_Identity=42.8571428571429, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790736, Length=329, Percent_Identity=42.5531914893617, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1787671, Length=329, Percent_Identity=42.5531914893617, Blast_Score=228, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 38800; Mature: 38668

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30 ; PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTELSVEERATIQIGRTQGFSLRRIACLINRSPSTISRELRRNRGACGGYSARLAQQQ
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
MQARRQVCRPMRKLLPGSERFELVTHMLRERLSPEQIAGKLRSMNIPNLRDAYVCRETIY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
NAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIPEMVSIHVRPPEIEDRLMPGH
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCC
WEGDLIKGKANASSVGTLVERTSGYLMLVKMNDATATSALEGFSAALNSMPLEMRKSMTY
CCCCEEECCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCH
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELD
HHHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
AIALQLNMRPRKRFDFKCPIEVMGEVMQNAMAMRHDAPVSIQ
EEHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYTELSVEERATIQIGRTQGFSLRRIACLINRSPSTISRELRRNRGACGGYSARLAQQQ
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
MQARRQVCRPMRKLLPGSERFELVTHMLRERLSPEQIAGKLRSMNIPNLRDAYVCRETIY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
NAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIPEMVSIHVRPPEIEDRLMPGH
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCC
WEGDLIKGKANASSVGTLVERTSGYLMLVKMNDATATSALEGFSAALNSMPLEMRKSMTY
CCCCEEECCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCH
DQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELD
HHHHHHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
AIALQLNMRPRKRFDFKCPIEVMGEVMQNAMAMRHDAPVSIQ
EEHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1334071 [H]