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The map label for this gene is panB

Identifier: 146283616

GI number: 146283616

Start: 3568187

End: 3568870

Strand: Direct

Name: panB

Synonym: PST_3296

Alternate gene names: 146283616

Gene position: 3568187-3568870 (Clockwise)

Preceding gene: 146283615

Following gene: 146283617

Centisome position: 78.12

GC content: 67.11

Gene sequence:

>684_bases
ATGCTGCTGATCGGCGACTCGCTCGGCATGGTCCTGCAAGGTCACGACAGCACGCTGCCGGTCACCGTCGCCGACATGGC
CTACCACACGGCCAGCGTCAAGCGTGGCAACCGCGGAGCGATGATCGTCGCCGACCTGCCGTTCATGGCCAACGCGACCA
CCGAGCAGACCCTGAACAATTCGGCGCTGCTGATGCAGGCCGGCGCCCACATGATCAAGCTCGAGGGCACCGCCTGGCTG
GCCGAGTCGATCCGCCTGCTGGCCGACCGCGGCATCCCGGTGTGCGCGCATATGGGGCTGACCCCGCAGGCGGTCAATCT
GTTCGGCGGCTACAAGGTGCAGGGGCGTGAAGAAGCCCAGGCACAGCAGATGCTGGAGGACGCCAAGTCGCTGGAAGCAG
CCGGCGCCGCCATGCTGCTGCTCGAATGCGTCCCCAGCGAACTGGCCGCGCGCATTACCCGGGCCGTGCAGATCCCGGTG
ATCGGCATCGGCGCCGGCAGCGATACCGACGGCCAGGTGCTGGTCCTGCATGACATGCTCGGACTGTCGCTCAGCGGCCG
CGTGCCGAAGTTCGTGAAGAACTTCATGCGCGAGCACGGCGACATCCCCGCGGCCATCGCCGGCTACGTCAAGGCGGTCA
AGGCCGTCGAATTCCCCGCAGCCGAACACGGTTTCTCCGCATGA

Upstream 100 bases:

>100_bases
GACCACGCTGCAAGGGCTCAAGCAGAAGGGCGAGAAGATCGTCATGCTCACCTGCTATGACGCCACCTTCGCCAAGACGG
CCTGCGATGCAGGCGTCGAG

Downstream 100 bases:

>100_bases
ACGTCGTCAAGAACATCGCCGACCTGCGCGCCGCGGTCGCCCGCGCGCGCGGCGAAGGCAAACGCATCGGTTTCGTGCCG
ACCATGGGCAACCTCCACGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MLLIGDSLGMVLQGHDSTLPVTVADMAYHTASVKRGNRGAMIVADLPFMANATTEQTLNNSALLMQAGAHMIKLEGTAWL
AESIRLLADRGIPVCAHMGLTPQAVNLFGGYKVQGREEAQAQQMLEDAKSLEAAGAAMLLLECVPSELAARITRAVQIPV
IGIGAGSDTDGQVLVLHDMLGLSLSGRVPKFVKNFMREHGDIPAAIAGYVKAVKAVEFPAAEHGFSA

Sequences:

>Translated_227_residues
MLLIGDSLGMVLQGHDSTLPVTVADMAYHTASVKRGNRGAMIVADLPFMANATTEQTLNNSALLMQAGAHMIKLEGTAWL
AESIRLLADRGIPVCAHMGLTPQAVNLFGGYKVQGREEAQAQQMLEDAKSLEAAGAAMLLLECVPSELAARITRAVQIPV
IGIGAGSDTDGQVLVLHDMLGLSLSGRVPKFVKNFMREHGDIPAAIAGYVKAVKAVEFPAAEHGFSA
>Mature_227_residues
MLLIGDSLGMVLQGHDSTLPVTVADMAYHTASVKRGNRGAMIVADLPFMANATTEQTLNNSALLMQAGAHMIKLEGTAWL
AESIRLLADRGIPVCAHMGLTPQAVNLFGGYKVQGREEAQAQQMLEDAKSLEAAGAAMLLLECVPSELAARITRAVQIPV
IGIGAGSDTDGQVLVLHDMLGLSLSGRVPKFVKNFMREHGDIPAAIAGYVKAVKAVEFPAAEHGFSA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=225, Percent_Identity=59.5555555555556, Blast_Score=290, Evalue=5e-80,
Organism=Saccharomyces cerevisiae, GI6319653, Length=235, Percent_Identity=38.7234042553192, Blast_Score=156, Evalue=3e-39,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PSEU5 (A4VPM6)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173769.1
- ProteinModelPortal:   A4VPM6
- SMR:   A4VPM6
- STRING:   A4VPM6
- GeneID:   5096257
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3296
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- BioCyc:   PSTU379731:PST_3296-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 23901; Mature: 23901

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLIGDSLGMVLQGHDSTLPVTVADMAYHTASVKRGNRGAMIVADLPFMANATTEQTLNN
CEEECCCCCEEEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHCCC
SALLMQAGAHMIKLEGTAWLAESIRLLADRGIPVCAHMGLTPQAVNLFGGYKVQGREEAQ
CEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEECCCHHHH
AQQMLEDAKSLEAAGAAMLLLECVPSELAARITRAVQIPVIGIGAGSDTDGQVLVLHDML
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCEEEEECCCCCCCCEEEEEECC
GLSLSGRVPKFVKNFMREHGDIPAAIAGYVKAVKAVEFPAAEHGFSA
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLLIGDSLGMVLQGHDSTLPVTVADMAYHTASVKRGNRGAMIVADLPFMANATTEQTLNN
CEEECCCCCEEEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHCCC
SALLMQAGAHMIKLEGTAWLAESIRLLADRGIPVCAHMGLTPQAVNLFGGYKVQGREEAQ
CEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEECCCHHHH
AQQMLEDAKSLEAAGAAMLLLECVPSELAARITRAVQIPVIGIGAGSDTDGQVLVLHDML
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCEEEEECCCCCCCCEEEEEECC
GLSLSGRVPKFVKNFMREHGDIPAAIAGYVKAVKAVEFPAAEHGFSA
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA