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The map label for this gene is mvaB [H]

Identifier: 146283542

GI number: 146283542

Start: 3474016

End: 3474915

Strand: Direct

Name: mvaB [H]

Synonym: PST_3217

Alternate gene names: 146283542

Gene position: 3474016-3474915 (Clockwise)

Preceding gene: 146283541

Following gene: 146283543

Centisome position: 76.06

GC content: 65.67

Gene sequence:

>900_bases
ATGAGCCTGCCCAAACAGGTCCGGCTGGTGGAAGTCGGCCCGCGTGATGGTCTGCAGAACGAGCAGCAGCCGATCAGCGT
GGCGGACAAGGTGCGTCTGGTCGACGACCTGAGCGCCGCCGGGCTGTCCTATATCGAAGTCGGCAGCTTCGTCTCGCCCA
AGTGGGTGCCGCAGATGGCCGGCTCCGCCGAAGTCTTCGCGCAGATCCAGCGCAAGGCCGGGGTCACTTACGCGGCGCTG
ACGCCGAACATGAAGGGCCTGGAGGCGGCCATCGAGGCCGGCGTGAAGGAAGTCGCGGTGTTCGGTGCGGCCTCCGAAGC
TTTTTCGCAGAAGAACATCAACTGCTCCATCGCCGAGAGCCTGGCACGCTTCGCGCCTTTGATGGAGGCTGCCCGCGAGC
AGGACATTCGCGTGCGTGGCTACGTTTCCTGCGTGCTCGGCTGCCCTTATGAGGGTGAGGTACCGCCAGCCAAGGTAGCC
GAAGTGGCCCGCGAGCTGTATGCCATGGGCTGCTACGAGGTTTCACTGGGCGACACCATCGGCACCGGCACACCGGGCAA
GACCCGCACGCTGTTCGATACCGTAGCCCGCGAAGTGCCGCGCGAACGGTTGGCCGGGCATTTCCACGACACCTACGGCC
AGGCACTGGCGAATATCTATGCGAGCCTCTTGGAAGGCATCGCGGTGTTCGACAGTTCGGTCGCCGGCCTCGGCGGTTGC
CCCTACGCCAAAGGCGCCAGCGGCAATGTCGCCAGCGAAGACGTGCTGTACATGTTCAACGGGCTGGACATAGCCACCGG
CATCGACCTCGACGCACTGATCGCCGCCGGCCAGCGCATCAGCCAGCTGCTGGGGCGTGCCAACGGTTCGCGGGTCGCCC
GCGCCAGGCATGCCGGTTAG

Upstream 100 bases:

>100_bases
GGGTGGAAAACGCCGCAGGCTTTTCCACCGGTAGCCGGACGCCTCGGCTGACACACATGAACCGGCGAGCCCGCCAATCG
CGCTCGCCCCTGGAGGACAT

Downstream 100 bases:

>100_bases
CGGCACCGTTCTTGCTAAACCCTATTCGTCGGGCGCCCATTCAGGTAGCGCCCGGCACGGACAGAACAACAAGTAGAGGT
AATTCATGAACAAGATTTAC

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSLPKQVRLVEVGPRDGLQNEQQPISVADKVRLVDDLSAAGLSYIEVGSFVSPKWVPQMAGSAEVFAQIQRKAGVTYAAL
TPNMKGLEAAIEAGVKEVAVFGAASEAFSQKNINCSIAESLARFAPLMEAAREQDIRVRGYVSCVLGCPYEGEVPPAKVA
EVARELYAMGCYEVSLGDTIGTGTPGKTRTLFDTVAREVPRERLAGHFHDTYGQALANIYASLLEGIAVFDSSVAGLGGC
PYAKGASGNVASEDVLYMFNGLDIATGIDLDALIAAGQRISQLLGRANGSRVARARHAG

Sequences:

>Translated_299_residues
MSLPKQVRLVEVGPRDGLQNEQQPISVADKVRLVDDLSAAGLSYIEVGSFVSPKWVPQMAGSAEVFAQIQRKAGVTYAAL
TPNMKGLEAAIEAGVKEVAVFGAASEAFSQKNINCSIAESLARFAPLMEAAREQDIRVRGYVSCVLGCPYEGEVPPAKVA
EVARELYAMGCYEVSLGDTIGTGTPGKTRTLFDTVAREVPRERLAGHFHDTYGQALANIYASLLEGIAVFDSSVAGLGGC
PYAKGASGNVASEDVLYMFNGLDIATGIDLDALIAAGQRISQLLGRANGSRVARARHAG
>Mature_298_residues
SLPKQVRLVEVGPRDGLQNEQQPISVADKVRLVDDLSAAGLSYIEVGSFVSPKWVPQMAGSAEVFAQIQRKAGVTYAALT
PNMKGLEAAIEAGVKEVAVFGAASEAFSQKNINCSIAESLARFAPLMEAAREQDIRVRGYVSCVLGCPYEGEVPPAKVAE
VARELYAMGCYEVSLGDTIGTGTPGKTRTLFDTVAREVPRERLAGHFHDTYGQALANIYASLLEGIAVFDSSVAGLGGCP
YAKGASGNVASEDVLYMFNGLDIATGIDLDALIAAGQRISQLLGRANGSRVARARHAG

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=294, Percent_Identity=60.5442176870748, Blast_Score=380, Evalue=1e-106,
Organism=Homo sapiens, GI109150427, Length=293, Percent_Identity=55.9726962457338, Blast_Score=352, Evalue=2e-97,
Organism=Homo sapiens, GI109150422, Length=293, Percent_Identity=55.9726962457338, Blast_Score=352, Evalue=2e-97,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=58.0882352941177, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17510563, Length=294, Percent_Identity=51.7006802721088, Blast_Score=305, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24582381, Length=294, Percent_Identity=58.843537414966, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 31526; Mature: 31395

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPKQVRLVEVGPRDGLQNEQQPISVADKVRLVDDLSAAGLSYIEVGSFVSPKWVPQMA
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHC
GSAEVFAQIQRKAGVTYAALTPNMKGLEAAIEAGVKEVAVFGAASEAFSQKNINCSIAES
CHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCHHHHHHHCCHHHHHHHCCCCHHHHHH
LARFAPLMEAAREQDIRVRGYVSCVLGCPYEGEVPPAKVAEVARELYAMGCYEVSLGDTI
HHHHHHHHHHHHHCCCCHHHHEEHEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
GTGTPGKTRTLFDTVAREVPRERLAGHFHDTYGQALANIYASLLEGIAVFDSSVAGLGGC
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PYAKGASGNVASEDVLYMFNGLDIATGIDLDALIAAGQRISQLLGRANGSRVARARHAG
CCCCCCCCCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
SLPKQVRLVEVGPRDGLQNEQQPISVADKVRLVDDLSAAGLSYIEVGSFVSPKWVPQMA
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHC
GSAEVFAQIQRKAGVTYAALTPNMKGLEAAIEAGVKEVAVFGAASEAFSQKNINCSIAES
CHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCHHHHHHHCCHHHHHHHCCCCHHHHHH
LARFAPLMEAAREQDIRVRGYVSCVLGCPYEGEVPPAKVAEVARELYAMGCYEVSLGDTI
HHHHHHHHHHHHHCCCCHHHHEEHEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
GTGTPGKTRTLFDTVAREVPRERLAGHFHDTYGQALANIYASLLEGIAVFDSSVAGLGGC
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PYAKGASGNVASEDVLYMFNGLDIATGIDLDALIAAGQRISQLLGRANGSRVARARHAG
CCCCCCCCCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]