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The map label for this gene is prsA [H]

Identifier: 146283514

GI number: 146283514

Start: 3434381

End: 3435310

Strand: Direct

Name: prsA [H]

Synonym: PST_3189

Alternate gene names: 146283514

Gene position: 3434381-3435310 (Clockwise)

Preceding gene: 146283513

Following gene: 146283515

Centisome position: 75.19

GC content: 60.22

Gene sequence:

>930_bases
ATGGTCTTCACGGGGAACGCCAACCCCGACCTGGCCCGGCGTGTCGTACGTCAGCTGCATATCCCCCTCGGTGATGCCTA
CGTCGGAAAATTCTCCGACGGCGAAATCAGTGTCGAGATCAACGAGAACGTTCGTGGTAAGGACGTCTTTCTGATTCAAC
CGACCTGCGCTCCGACCAACGACAACCTGATGGAACTGGTAGTGCTGGCTGACGCCTTCCGCCGCTCCTCGGCGTCGCGA
ATCACCGCTGTGATCCCCTACTTCGGTTATGCCCGCCAGGATCGTCGCCCGCGCTCTGCTCGTGTGCCGATCAGCGCCAA
GGTCGTGGCCGACATGCTCGACGTCGTGGGCGTCAACCGTGTTCTGACCGTCGACCTGCACGCCGACCAGATCCAGGGTT
TCTTCGACATGCCGGTGGACAACATCTACGGCTCGCCGGTACTGGTCGACGACATCCAGGCTCAGCGCTTCGACAACCTG
ATGATCGTCTCCCCCGATATCGGCGGCGTAGTTCGAGCCCGTGCCGTAGCCAAGTCCCTTGGCGTCGACCTGGCGATCAT
CGACAAGCGTCGACCAAAGGCCAACCAGTCGGAAGTGATGCACATCATCGGTGACATCGATAGCCGTACCTGCATCCTCG
TCGACGACATGGTCGATACCGCCGGTACCCTGTGTCACGCAGCCAAGGCACTGAAAGATCATGGCGCTGCCAAGGTCTAC
GCCTACTGCACACACCCGATCCTGTCCGGTCGTGCCATCGAGAATATCGAGGGTTCAGTGCTCGACGAGCTGGTGGTGAC
CAACACCATTCCGCTGTCGGCTGCTGCACAGTCCTGTACCCGAATTCGCCAACTGGACATCGCGCCAGTGGTGGCTGAAG
CGGTTCGCCGCATCAGCAATGCCGAATCGATCAGTGCGATGTTCCGCTAA

Upstream 100 bases:

>100_bases
GCCCCTGCCATACACTTGAAGCTGTTCAAGAGCTTGCCTTTTGAACAGCTTTTTATTCATCGGACCCACCCTCAGGCAGG
TACTGCGCGTGTCCAAGATG

Downstream 100 bases:

>100_bases
GCGAAAGCTACGCAAATCGCACTGAACGTAATGTGCCCCGCCTCGTGCGGGGCTTTTTGCCCAACAGCCCGAGCGCTGGT
CGCAAGCGCGACAGGTAATG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MVFTGNANPDLARRVVRQLHIPLGDAYVGKFSDGEISVEINENVRGKDVFLIQPTCAPTNDNLMELVVLADAFRRSSASR
ITAVIPYFGYARQDRRPRSARVPISAKVVADMLDVVGVNRVLTVDLHADQIQGFFDMPVDNIYGSPVLVDDIQAQRFDNL
MIVSPDIGGVVRARAVAKSLGVDLAIIDKRRPKANQSEVMHIIGDIDSRTCILVDDMVDTAGTLCHAAKALKDHGAAKVY
AYCTHPILSGRAIENIEGSVLDELVVTNTIPLSAAAQSCTRIRQLDIAPVVAEAVRRISNAESISAMFR

Sequences:

>Translated_309_residues
MVFTGNANPDLARRVVRQLHIPLGDAYVGKFSDGEISVEINENVRGKDVFLIQPTCAPTNDNLMELVVLADAFRRSSASR
ITAVIPYFGYARQDRRPRSARVPISAKVVADMLDVVGVNRVLTVDLHADQIQGFFDMPVDNIYGSPVLVDDIQAQRFDNL
MIVSPDIGGVVRARAVAKSLGVDLAIIDKRRPKANQSEVMHIIGDIDSRTCILVDDMVDTAGTLCHAAKALKDHGAAKVY
AYCTHPILSGRAIENIEGSVLDELVVTNTIPLSAAAQSCTRIRQLDIAPVVAEAVRRISNAESISAMFR
>Mature_309_residues
MVFTGNANPDLARRVVRQLHIPLGDAYVGKFSDGEISVEINENVRGKDVFLIQPTCAPTNDNLMELVVLADAFRRSSASR
ITAVIPYFGYARQDRRPRSARVPISAKVVADMLDVVGVNRVLTVDLHADQIQGFFDMPVDNIYGSPVLVDDIQAQRFDNL
MIVSPDIGGVVRARAVAKSLGVDLAIIDKRRPKANQSEVMHIIGDIDSRTCILVDDMVDTAGTLCHAAKALKDHGAAKVY
AYCTHPILSGRAIENIEGSVLDELVVTNTIPLSAAAQSCTRIRQLDIAPVVAEAVRRISNAESISAMFR

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=49.6774193548387, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI84875539, Length=312, Percent_Identity=49.0384615384615, Blast_Score=311, Evalue=3e-85,
Organism=Homo sapiens, GI4506127, Length=309, Percent_Identity=48.5436893203884, Blast_Score=308, Evalue=6e-84,
Organism=Homo sapiens, GI28557709, Length=309, Percent_Identity=47.2491909385113, Blast_Score=300, Evalue=9e-82,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=35.8600583090379, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI4506133, Length=345, Percent_Identity=34.4927536231884, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=32.8859060402685, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=32.8859060402685, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=32.8859060402685, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=32.8859060402685, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787458, Length=308, Percent_Identity=67.8571428571429, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17554704, Length=309, Percent_Identity=46.6019417475728, Blast_Score=301, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI25149168, Length=309, Percent_Identity=46.6019417475728, Blast_Score=300, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17554702, Length=309, Percent_Identity=46.6019417475728, Blast_Score=300, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI71989924, Length=309, Percent_Identity=46.6019417475728, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=34.3195266272189, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6319403, Length=311, Percent_Identity=45.6591639871383, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6320946, Length=310, Percent_Identity=44.5161290322581, Blast_Score=268, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6321776, Length=301, Percent_Identity=45.514950166113, Blast_Score=265, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=41.3265306122449, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=114, Percent_Identity=42.9824561403509, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21355239, Length=309, Percent_Identity=49.5145631067961, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI45551540, Length=331, Percent_Identity=46.2235649546828, Blast_Score=292, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=35.0282485875706, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=35.0282485875706, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI281362873, Length=354, Percent_Identity=35.0282485875706, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24651454, Length=354, Percent_Identity=35.0282485875706, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24651462, Length=201, Percent_Identity=37.8109452736318, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24651464, Length=201, Percent_Identity=37.8109452736318, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45552010, Length=201, Percent_Identity=37.8109452736318, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33617; Mature: 33617

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFTGNANPDLARRVVRQLHIPLGDAYVGKFSDGEISVEINENVRGKDVFLIQPTCAPTN
CEECCCCCHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCC
DNLMELVVLADAFRRSSASRITAVIPYFGYARQDRRPRSARVPISAKVVADMLDVVGVNR
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCE
VLTVDLHADQIQGFFDMPVDNIYGSPVLVDDIQAQRFDNLMIVSPDIGGVVRARAVAKSL
EEEEEEEHHHHCCCCCCCHHHHCCCCEEEECHHHHHCCCEEEECCCCCHHHHHHHHHHHH
GVDLAIIDKRRPKANQSEVMHIIGDIDSRTCILVDDMVDTAGTLCHAAKALKDHGAAKVY
CCCEEEEECCCCCCCHHHHHHHEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEE
AYCTHPILSGRAIENIEGSVLDELVVTNTIPLSAAAQSCTRIRQLDIAPVVAEAVRRISN
EEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
AESISAMFR
HHHHHHHCC
>Mature Secondary Structure
MVFTGNANPDLARRVVRQLHIPLGDAYVGKFSDGEISVEINENVRGKDVFLIQPTCAPTN
CEECCCCCHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCC
DNLMELVVLADAFRRSSASRITAVIPYFGYARQDRRPRSARVPISAKVVADMLDVVGVNR
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCE
VLTVDLHADQIQGFFDMPVDNIYGSPVLVDDIQAQRFDNLMIVSPDIGGVVRARAVAKSL
EEEEEEEHHHHCCCCCCCHHHHCCCCEEEECHHHHHCCCEEEECCCCCHHHHHHHHHHHH
GVDLAIIDKRRPKANQSEVMHIIGDIDSRTCILVDDMVDTAGTLCHAAKALKDHGAAKVY
CCCEEEEECCCCCCCHHHHHHHEECCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEE
AYCTHPILSGRAIENIEGSVLDELVVTNTIPLSAAAQSCTRIRQLDIAPVVAEAVRRISN
EEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
AESISAMFR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]