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The map label for this gene is emrA [H]

Identifier: 146283430

GI number: 146283430

Start: 3341177

End: 3342292

Strand: Direct

Name: emrA [H]

Synonym: PST_3103

Alternate gene names: 146283430

Gene position: 3341177-3342292 (Clockwise)

Preceding gene: 146283429

Following gene: 146283432

Centisome position: 73.15

GC content: 71.33

Gene sequence:

>1116_bases
ATGAGCGATGCGCGCACACCCGACGCCGAGACCACGCCGACCACGGACGCCGGCGGCACACCGCCCGAGCCACCCAAGAC
GATCCAGCCAAGCCGCCGCTCGATCATCCTGATGACGCTGGTGGCCCTGGTCGGCGTGCTCGCCATCCTCTATGCCTGGC
AGCTCTGGCCGTTCACCGGCAGCCTGGTCACCACCGAGAACGCCTATGTGCGCGGCCAGCTCACGGTGCTCGCGCCGCAG
GTCGCCGGCTACGTCACCGAGGTGCGGGTGCAGGACTTCGAGCAGGTCCGCCAGGGCCAGGTGCTGGTACGCATCGATGA
CAGGCAGTACCAGCAACGGGTCGCCCAGCGCCGCGCCGAACGGGACGCCGCACGCTTCGAACTGGAAAACCTCGACCAGC
AACTGGCCGCCGACCGCGCCACCCTGGCCGCGCGCCGCGCCGACCTGTTCGCCGCCGAGGCCGAAGGGGCACGGGCACGG
GCCGACGAGCGTCGCGTCGACGAACTCGCCGAGCGCGGCTCGGTGTCCATCCGCGAGCGCGACCAGACCCGCGCCAGCGC
CCGCGCCGCGTCGGCCAACGTGCGCAAGGCACGCGCCGCCATCGAGATCGCCGAGCAGACGCTCAAGGCCAGCGGCATCT
CCCGGGGCGGCCTGCAGGCCCGGGTCGAGATCGCCCAGGCAACGCTGCACCAGGCCGAGATCGACCTGGCCAACACCGAG
ATCGTCGCCCCTGGCGATGGGCGGGTCAGCGAGGTGGGCGTGCGCCTGGGCCAGTACGTTACTGCCGGCACGCAGCTGCT
GTTCCTGGTGCCGCCGAAGCTCTGGGTGATCGCCAACTACAAGGAGACCCAGACGGCGAACATGCGCCCCGGCCAGCCGG
TGAGTTTCGAGGTCGATGCCCTCAACGGCGCGCGCCTGACCGGCCGCATCGAGCGCATCGCCCCGGCCACGGGCTCCGAG
TTCAGCCTGCTGCGCCCGGACAACGCAACCGGCAATTTCACCAAGGTGGTCCAGCGGCTGCCGGTACGCATCGCCATCGA
CCCGGACCAGCCGCTGCTCGAGCGCTTGCGGCCGGGGTTGTCGGTGGTCACCCACGTGGACACCGCGGCGCCATGA

Upstream 100 bases:

>100_bases
ATGACGTGTTCCGCCTGATCGGCCTGCTCGCCATCGCCGTGCTCGGCTGGTCACTGTTCCACACCCTGCGCCTGGCGCGA
CAGAAGAAGGCTTCCGCCCC

Downstream 100 bases:

>100_bases
GGAAAACCGGTCTGCTCGGCACGCTGGCGCTGACGCTGGTGATGGCCGGCTGCGTGCCTCCGCGCCAGCCGCTGCCACCC
GAAGCCCGGCTGGACGAGGT

Product: multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSDARTPDAETTPTTDAGGTPPEPPKTIQPSRRSIILMTLVALVGVLAILYAWQLWPFTGSLVTTENAYVRGQLTVLAPQ
VAGYVTEVRVQDFEQVRQGQVLVRIDDRQYQQRVAQRRAERDAARFELENLDQQLAADRATLAARRADLFAAEAEGARAR
ADERRVDELAERGSVSIRERDQTRASARAASANVRKARAAIEIAEQTLKASGISRGGLQARVEIAQATLHQAEIDLANTE
IVAPGDGRVSEVGVRLGQYVTAGTQLLFLVPPKLWVIANYKETQTANMRPGQPVSFEVDALNGARLTGRIERIAPATGSE
FSLLRPDNATGNFTKVVQRLPVRIAIDPDQPLLERLRPGLSVVTHVDTAAP

Sequences:

>Translated_371_residues
MSDARTPDAETTPTTDAGGTPPEPPKTIQPSRRSIILMTLVALVGVLAILYAWQLWPFTGSLVTTENAYVRGQLTVLAPQ
VAGYVTEVRVQDFEQVRQGQVLVRIDDRQYQQRVAQRRAERDAARFELENLDQQLAADRATLAARRADLFAAEAEGARAR
ADERRVDELAERGSVSIRERDQTRASARAASANVRKARAAIEIAEQTLKASGISRGGLQARVEIAQATLHQAEIDLANTE
IVAPGDGRVSEVGVRLGQYVTAGTQLLFLVPPKLWVIANYKETQTANMRPGQPVSFEVDALNGARLTGRIERIAPATGSE
FSLLRPDNATGNFTKVVQRLPVRIAIDPDQPLLERLRPGLSVVTHVDTAAP
>Mature_370_residues
SDARTPDAETTPTTDAGGTPPEPPKTIQPSRRSIILMTLVALVGVLAILYAWQLWPFTGSLVTTENAYVRGQLTVLAPQV
AGYVTEVRVQDFEQVRQGQVLVRIDDRQYQQRVAQRRAERDAARFELENLDQQLAADRATLAARRADLFAAEAEGARARA
DERRVDELAERGSVSIRERDQTRASARAASANVRKARAAIEIAEQTLKASGISRGGLQARVEIAQATLHQAEIDLANTEI
VAPGDGRVSEVGVRLGQYVTAGTQLLFLVPPKLWVIANYKETQTANMRPGQPVSFEVDALNGARLTGRIERIAPATGSEF
SLLRPDNATGNFTKVVQRLPVRIAIDPDQPLLERLRPGLSVVTHVDTAAP

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=340, Percent_Identity=31.4705882352941, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789041, Length=355, Percent_Identity=32.3943661971831, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790519, Length=317, Percent_Identity=28.7066246056782, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=27.6527331189711, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790024, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790012, Length=246, Percent_Identity=27.2357723577236, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789900, Length=338, Percent_Identity=26.3313609467456, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40476; Mature: 40345

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDARTPDAETTPTTDAGGTPPEPPKTIQPSRRSIILMTLVALVGVLAILYAWQLWPFTG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLVTTENAYVRGQLTVLAPQVAGYVTEVRVQDFEQVRQGQVLVRIDDRQYQQRVAQRRAE
CEEEECCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
RDAARFELENLDQQLAADRATLAARRADLFAAEAEGARARADERRVDELAERGSVSIRER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEHH
DQTRASARAASANVRKARAAIEIAEQTLKASGISRGGLQARVEIAQATLHQAEIDLANTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCE
IVAPGDGRVSEVGVRLGQYVTAGTQLLFLVPPKLWVIANYKETQTANMRPGQPVSFEVDA
EECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEEEC
LNGARLTGRIERIAPATGSEFSLLRPDNATGNFTKVVQRLPVRIAIDPDQPLLERLRPGL
CCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHCCCC
SVVTHVDTAAP
EEEEECCCCCC
>Mature Secondary Structure 
SDARTPDAETTPTTDAGGTPPEPPKTIQPSRRSIILMTLVALVGVLAILYAWQLWPFTG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLVTTENAYVRGQLTVLAPQVAGYVTEVRVQDFEQVRQGQVLVRIDDRQYQQRVAQRRAE
CEEEECCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
RDAARFELENLDQQLAADRATLAARRADLFAAEAEGARARADERRVDELAERGSVSIRER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEHH
DQTRASARAASANVRKARAAIEIAEQTLKASGISRGGLQARVEIAQATLHQAEIDLANTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCE
IVAPGDGRVSEVGVRLGQYVTAGTQLLFLVPPKLWVIANYKETQTANMRPGQPVSFEVDA
EECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEEEC
LNGARLTGRIERIAPATGSEFSLLRPDNATGNFTKVVQRLPVRIAIDPDQPLLERLRPGL
CCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHCCCC
SVVTHVDTAAP
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]