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The map label for this gene is leuA

Identifier: 146283346

GI number: 146283346

Start: 3248084

End: 3249754

Strand: Direct

Name: leuA

Synonym: PST_3016

Alternate gene names: 146283346

Gene position: 3248084-3249754 (Clockwise)

Preceding gene: 146283343

Following gene: 146283347

Centisome position: 71.11

GC content: 63.08

Gene sequence:

>1671_bases
ATGACCATGCTCAAGAATCCATCGAATAAATACCGTGCCTTCCCGGCCATCGACATTCCCGATCGCACCTGGCCGTCGAA
GGCCATCACCCAGGCGCCGATCTGGCTGAGCTCCGATCTGCGCGACGGCAACCAGTCGCTGATCGAACCGATGGACGCCG
CGAAGAAGATGCGTTTCTTCAAGACGCTGGTGCAGGTCGGCCTCAAGGAAATCGAGGTGGGCTTCCCCTCCGCTTCGCAG
ACCGATTTCGACTTCGTTCGCGAGCTGATCGAGGGCGGCCATATTCCCGACGACGTCACCATCCAGGTGCTGACCCAGGC
GCGAGAGGATCTGATCACCCGCACCTTCGAGTCGCTGAAAGGCGCCAAGCAGGCCATCGTCCATTACTACAACGCCACCG
CGCCGAGCTTCCGCCGCATCGTCTTCAATCAGGACAAGGCCGGCGTGATCCAGATCGCCGTGAACGCAGGCAGGATCATC
AAGCGCCTGGCCGAGCAGAACCCGGAGACCAACTGGCGCTTCGAGTATTCGCCGGAAATCTTCAGCTCCACCGAGCCGGA
GTTCGCCGTCGAGGTGTGCAACGCGGTGATCGAGGTGTTCCAGCCAACGCCGGAACAGAAGCTGATCCTCAACCTGCCGG
CCACGGTGGAAGCCGCCACGCCGAATGTCTATGCCGACCAGATCGAGTGGTTCTGCCGGCACATCGACCGTCGCGACAGC
GTGCTGGTAAGCCTGCACACCCACAACGACCGCGGCACCGGTGTGGCCGCCACCGAGCTGGGCCTGATGGCCGGCGCCGA
TCGCGTCGAAGGCTGCCTGTTCGGCAACGGCGAGCGTACCGGCAACGTCGACCTGGTCACCGTGGCGCTGAACATGTACA
CCCAGGGCGTCGACCCGCAGCTGGACTTCTCGGACATCGATGCCGTGCGCAAGGTGGTGGAGGAATGCAATCAGCTGCCG
GTGCATCCGCGCCATCCCTATGTCGGCGACCTGGTGCACACCGCGTTCTCCGGCTCGCACCAGGATGCGATCCGCAAGGG
CTTCAGCCAGCAGGACCCGAACGGCCTCTGGGAAGTGCCCTATCTGCCGATCGACCCGGCCGACATCGGTCGCAGTTACG
AGGCCGTGATTCGCGTCAACAGCCAGTCCGGAAAAGGTGGCATCACATTCCTCCTCGAACAGGAATACGGCATCAGCCTG
CCGCGGCGCATGCAGATCGAGTTCAGCCAGGTGGTGCAGAAGGAGACCGATCGGCTGGGCCTGGAAATGAGCGCCAAGCA
GATCTATGCGCTGCTGGAAGCCGAGTATCTGCAGGCAACCGCGCCCTATACGCTCAAGGGGCATCGTCTGCAGGAAGAGA
ACGGCACCAGCGCGGTGGATGTGGAAGTGGCTGCCGATGGCGAGGTCATCCATTGGCGCGGCATCGGCAAGGGTCCGCTG
GAGGCACTGGTGGCCGCGTTGCCGGTCAAGCTGGAGATCATGGACTACCATGAACACGCCATCGGCGCTGGCAGCAACGC
CAAGGCCGCTGCCTATATCGAGGTGCGTCTGGATGGTGAGCGGCCATTGCACGGTATCGGCATCGACGAGAACATCACCA
CCGCCAGCATTCGCGCCCTGTTCAGCGCCATGAACCGCGCGCTGCATCAAGCGCAGGAGGCAGCCGCCTGA

Upstream 100 bases:

>100_bases
ATTGCAATGCGCGCGTACGAAGCAATAGCTTTGCACCATAAACCGGTGTCCGCACCGGGCAGACAACTTGCTTAGCTCAA
GCTTCACCTGGGGAAACGCC

Downstream 100 bases:

>100_bases
GGCTGATCGCTCAGTTCTGACGTGACGACGGGCGCCATCCTCGATGGCGCCCGTTTTCTTATGGCAGCTCATCACCGCAG
CAACATGCGGCTGAACTGTC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTMLKNPSNKYRAFPAIDIPDRTWPSKAITQAPIWLSSDLRDGNQSLIEPMDAAKKMRFFKTLVQVGLKEIEVGFPSASQ
TDFDFVRELIEGGHIPDDVTIQVLTQAREDLITRTFESLKGAKQAIVHYYNATAPSFRRIVFNQDKAGVIQIAVNAGRII
KRLAEQNPETNWRFEYSPEIFSSTEPEFAVEVCNAVIEVFQPTPEQKLILNLPATVEAATPNVYADQIEWFCRHIDRRDS
VLVSLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVDPQLDFSDIDAVRKVVEECNQLP
VHPRHPYVGDLVHTAFSGSHQDAIRKGFSQQDPNGLWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGITFLLEQEYGISL
PRRMQIEFSQVVQKETDRLGLEMSAKQIYALLEAEYLQATAPYTLKGHRLQEENGTSAVDVEVAADGEVIHWRGIGKGPL
EALVAALPVKLEIMDYHEHAIGAGSNAKAAAYIEVRLDGERPLHGIGIDENITTASIRALFSAMNRALHQAQEAAA

Sequences:

>Translated_556_residues
MTMLKNPSNKYRAFPAIDIPDRTWPSKAITQAPIWLSSDLRDGNQSLIEPMDAAKKMRFFKTLVQVGLKEIEVGFPSASQ
TDFDFVRELIEGGHIPDDVTIQVLTQAREDLITRTFESLKGAKQAIVHYYNATAPSFRRIVFNQDKAGVIQIAVNAGRII
KRLAEQNPETNWRFEYSPEIFSSTEPEFAVEVCNAVIEVFQPTPEQKLILNLPATVEAATPNVYADQIEWFCRHIDRRDS
VLVSLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVDPQLDFSDIDAVRKVVEECNQLP
VHPRHPYVGDLVHTAFSGSHQDAIRKGFSQQDPNGLWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGITFLLEQEYGISL
PRRMQIEFSQVVQKETDRLGLEMSAKQIYALLEAEYLQATAPYTLKGHRLQEENGTSAVDVEVAADGEVIHWRGIGKGPL
EALVAALPVKLEIMDYHEHAIGAGSNAKAAAYIEVRLDGERPLHGIGIDENITTASIRALFSAMNRALHQAQEAAA
>Mature_555_residues
TMLKNPSNKYRAFPAIDIPDRTWPSKAITQAPIWLSSDLRDGNQSLIEPMDAAKKMRFFKTLVQVGLKEIEVGFPSASQT
DFDFVRELIEGGHIPDDVTIQVLTQAREDLITRTFESLKGAKQAIVHYYNATAPSFRRIVFNQDKAGVIQIAVNAGRIIK
RLAEQNPETNWRFEYSPEIFSSTEPEFAVEVCNAVIEVFQPTPEQKLILNLPATVEAATPNVYADQIEWFCRHIDRRDSV
LVSLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVDPQLDFSDIDAVRKVVEECNQLPV
HPRHPYVGDLVHTAFSGSHQDAIRKGFSQQDPNGLWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGITFLLEQEYGISLP
RRMQIEFSQVVQKETDRLGLEMSAKQIYALLEAEYLQATAPYTLKGHRLQEENGTSAVDVEVAADGEVIHWRGIGKGPLE
ALVAALPVKLEIMDYHEHAIGAGSNAKAAAYIEVRLDGERPLHGIGIDENITTASIRALFSAMNRALHQAQEAAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=532, Percent_Identity=24.0601503759398, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=571, Percent_Identity=51.6637478108581, Blast_Score=591, Evalue=1e-169,
Organism=Saccharomyces cerevisiae, GI6324682, Length=569, Percent_Identity=50.7908611599297, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_PSEU5 (A4VNV6)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001173499.1
- ProteinModelPortal:   A4VNV6
- SMR:   A4VNV6
- STRING:   A4VNV6
- GeneID:   5095978
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3016
- eggNOG:   COG0119
- HOGENOM:   HBG289752
- OMA:   VERCNQI
- PhylomeDB:   A4VNV6
- ProtClustDB:   PRK03739
- BioCyc:   PSTU379731:PST_3016-MONOMER
- HAMAP:   MF_00572
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF2
- SMART:   SM00917
- TIGRFAMs:   TIGR00970

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 61568; Mature: 61437

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLKNPSNKYRAFPAIDIPDRTWPSKAITQAPIWLSSDLRDGNQSLIEPMDAAKKMRFF
CCCCCCCCCCEEECCCCCCCCCCCCCHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
KTLVQVGLKEIEVGFPSASQTDFDFVRELIEGGHIPDDVTIQVLTQAREDLITRTFESLK
HHHHHHCHHHEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GAKQAIVHYYNATAPSFRRIVFNQDKAGVIQIAVNAGRIIKRLAEQNPETNWRFEYSPEI
HHHHHHHHHHCCCCCHHHEEEECCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEECCHH
FSSTEPEFAVEVCNAVIEVFQPTPEQKLILNLPATVEAATPNVYADQIEWFCRHIDRRDS
HCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCC
VLVSLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVDPQ
EEEEEEECCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDAVRKVVEECNQLPVHPRHPYVGDLVHTAFSGSHQDAIRKGFSQQDPNGLWEVP
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEECC
YLPIDPADIGRSYEAVIRVNSQSGKGGITFLLEQEYGISLPRRMQIEFSQVVQKETDRLG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEECHHHHHHHHHHHHHCC
LEMSAKQIYALLEAEYLQATAPYTLKGHRLQEENGTSAVDVEVAADGEVIHWRGIGKGPL
CCHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCCEEEEEEECCCCEEEEECCCCCHH
EALVAALPVKLEIMDYHEHAIGAGSNAKAAAYIEVRLDGERPLHGIGIDENITTASIRAL
HHHHHHCCEEEEEEECHHHHCCCCCCCCEEEEEEEEECCCCCEECCCCCCCCHHHHHHHH
FSAMNRALHQAQEAAA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMLKNPSNKYRAFPAIDIPDRTWPSKAITQAPIWLSSDLRDGNQSLIEPMDAAKKMRFF
CCCCCCCCCEEECCCCCCCCCCCCCHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
KTLVQVGLKEIEVGFPSASQTDFDFVRELIEGGHIPDDVTIQVLTQAREDLITRTFESLK
HHHHHHCHHHEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GAKQAIVHYYNATAPSFRRIVFNQDKAGVIQIAVNAGRIIKRLAEQNPETNWRFEYSPEI
HHHHHHHHHHCCCCCHHHEEEECCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEECCHH
FSSTEPEFAVEVCNAVIEVFQPTPEQKLILNLPATVEAATPNVYADQIEWFCRHIDRRDS
HCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCC
VLVSLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVDPQ
EEEEEEECCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDAVRKVVEECNQLPVHPRHPYVGDLVHTAFSGSHQDAIRKGFSQQDPNGLWEVP
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEECC
YLPIDPADIGRSYEAVIRVNSQSGKGGITFLLEQEYGISLPRRMQIEFSQVVQKETDRLG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEECHHHHHHHHHHHHHCC
LEMSAKQIYALLEAEYLQATAPYTLKGHRLQEENGTSAVDVEVAADGEVIHWRGIGKGPL
CCHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCCEEEEEEECCCCEEEEECCCCCHH
EALVAALPVKLEIMDYHEHAIGAGSNAKAAAYIEVRLDGERPLHGIGIDENITTASIRAL
HHHHHHCCEEEEEEECHHHHCCCCCCCCEEEEEEEEECCCCCEECCCCCCCCHHHHHHHH
FSAMNRALHQAQEAAA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA