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The map label for this gene is topB [H]

Identifier: 146283248

GI number: 146283248

Start: 3134600

End: 3136447

Strand: Direct

Name: topB [H]

Synonym: PST_2913

Alternate gene names: 146283248

Gene position: 3134600-3136447 (Clockwise)

Preceding gene: 146283247

Following gene: 146283251

Centisome position: 68.63

GC content: 69.1

Gene sequence:

>1848_bases
GTGACGGTGACCTGGTGCATCGGCCATCTGCTGGAGACCGCCCCGCCGGACGCCTATGACCCGCGCTACAAGCGCTGGGT
GCTGGCCGACCTGCCGATCGTGCCGGAGCGCTGGAAGATGCTGGTCAAGCCGAAGACCGCCAGCCAGTTCAAGGCGGTCA
AGCGTCTGCTCGGTGAATGCAGGGAACTGGTGATCGCCACCGACGCCGACCGCGAAGGCGAGATGATCGCCCGCGAGCTG
GTTGAGCACTGCCGCTACCGCGGGCCCATCCGCCGGCTGTGGCTGTCGGCGCTGGACGATGCCTCGATCCGCAAGGCGCT
GGCTGCGCTCAAGCCCGGCGCCGAGACCTTCAACCTCTACCACGCCGCCCTCGGCCGCTCGCGTGCTGACTGGCTGATCG
GCATGAACATGAGCCGGCTGTTCACCCTGCTCGGCCGCCAGTCCGGCTACCGCGGCGTGCTGCCGGTGGGCCGCGTGCAG
ACGCCGACGCTGCGTCTGGTGGTGGACCGCGACCGCAGCATCGCCGATTTCATCCCGGTGCCGTTCTGGGCCATCGACGT
GCAGCTGCTCGCCGATGGCGCCGCGTTCACCGCGCAGTGGCAGGCGCCGGACGACCTTTGCGATGACCAGGGCCGCTGCC
TCGATCAGGCTCGTGCGCAGCAGGCTGCGGACGCCATGCGCAACGCCGCCAGCGCTCGCCTGCTGAAGCTCAAGACCGAG
CGGCAGCGCGAGGCGGCACCCTTGCCGTTCGATCTCGGCACGCTGCAGGAAGTCTGCTCGAAGAAGCTCGGCCTCGGCGC
CCAGGAAACCCTCGACATCGCCCAGGCGCTGTACGAAACCCACAAACTGATCACCTATCCGCGCAGCGATTGCGGCTACC
TGCCACTGAGCCAGCTCGGCGAAGCGCGGGCCATCGTCGCGGCGCTGACTCAAGCCGACCCGACGCTCGCGGCGCTGCAG
CCGCATCTGGACCTGTCGCGCCGCTCGCGGGCGTGGAATGACGCCAAGGTCGGCGCCCACCACGGCATCGTCCCAACCGC
CGCCGCGCGTGGTCTGGAACGCCTGGCCGGGCGCCCGCGTGCGGTGTATGAGCTGATCCGCGCGCGCTACCTGGCACAGT
TCCTGCCCAACCACGAGTACGACCGCACCCAGGCTGACTTCGACTGCGCCGGCCAGGCGCTGCGCGCGGTGGGCAAGCGC
GTCGTCGAACCGGGCTGGAAACGCGCCATGCCCGAAGCACTGGCGCCGGCACGGGGCAATCGCGAGGCACATGCGCCGCA
AAGCCTGCCGGCGCTGCAGCAGGGCCAGGACTATGCCGTGGGCGAGATCACACTCAAGGACCAGCAGACACAACCGCCGA
AGCCCTTCACCGAAGGCGACCTGATCAAGGCAATGAAGAACGTCGCCAAGCTCGTGGACGACCCGCGGCTGAAGCAGAAA
CTCAAGGACACCACCGGCATCGGCACCGAGGCGACCCGCGCCGGGATAATTCAGGGCCTGCTCGACCGCGGCTATCTGGT
TCGCCAGGGCAAGGCGCTGGCGGCGACACCGGCGGCGTTCAGCCTGATCGACGCCGTGCCGCGGCCGATCGCCGACCCGG
GTACCACGGCGATCTGGGAGCAGGCGCTGGACATGGTGCAGAGCGGCGAAATGCCGTTGGAGGAATTCGTCGCCAAGCAA
TCGGCGTGGATGAGCAAGCTGGTCGAACGCTGCGCCGGACTGCGCATGACCATCAGCGGCCCGCCGGTGGCCGCCGGCCG
CAACGGCAAACCGTGGAAGAAGAAACGCAGCGCCACGCCACGCAGAGGGAGCGGCGCTCGGACGAGAAAACCGCGCGCGG
CCGATTGA

Upstream 100 bases:

>100_bases
CATGCAACTCTATCTCTGCGAAAAGCCTTCCCAGGCCCGCGACATCGCCAAGGTGCTCGGCGCCAACCGGCCCGGCGACG
GCTGCCTGCAGGGCGCCGGG

Downstream 100 bases:

>100_bases
GCGGGCCTTTGCGCCATGCCCCACAGCGCCGTTGAAACCCATTTGCACCCGTTGGGCGGGCATTAGCCCCTAATAGCCGG
CACTATGCGATCTGCAGCAC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MTVTWCIGHLLETAPPDAYDPRYKRWVLADLPIVPERWKMLVKPKTASQFKAVKRLLGECRELVIATDADREGEMIAREL
VEHCRYRGPIRRLWLSALDDASIRKALAALKPGAETFNLYHAALGRSRADWLIGMNMSRLFTLLGRQSGYRGVLPVGRVQ
TPTLRLVVDRDRSIADFIPVPFWAIDVQLLADGAAFTAQWQAPDDLCDDQGRCLDQARAQQAADAMRNAASARLLKLKTE
RQREAAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETHKLITYPRSDCGYLPLSQLGEARAIVAALTQADPTLAALQ
PHLDLSRRSRAWNDAKVGAHHGIVPTAAARGLERLAGRPRAVYELIRARYLAQFLPNHEYDRTQADFDCAGQALRAVGKR
VVEPGWKRAMPEALAPARGNREAHAPQSLPALQQGQDYAVGEITLKDQQTQPPKPFTEGDLIKAMKNVAKLVDDPRLKQK
LKDTTGIGTEATRAGIIQGLLDRGYLVRQGKALAATPAAFSLIDAVPRPIADPGTTAIWEQALDMVQSGEMPLEEFVAKQ
SAWMSKLVERCAGLRMTISGPPVAAGRNGKPWKKKRSATPRRGSGARTRKPRAAD

Sequences:

>Translated_615_residues
MTVTWCIGHLLETAPPDAYDPRYKRWVLADLPIVPERWKMLVKPKTASQFKAVKRLLGECRELVIATDADREGEMIAREL
VEHCRYRGPIRRLWLSALDDASIRKALAALKPGAETFNLYHAALGRSRADWLIGMNMSRLFTLLGRQSGYRGVLPVGRVQ
TPTLRLVVDRDRSIADFIPVPFWAIDVQLLADGAAFTAQWQAPDDLCDDQGRCLDQARAQQAADAMRNAASARLLKLKTE
RQREAAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETHKLITYPRSDCGYLPLSQLGEARAIVAALTQADPTLAALQ
PHLDLSRRSRAWNDAKVGAHHGIVPTAAARGLERLAGRPRAVYELIRARYLAQFLPNHEYDRTQADFDCAGQALRAVGKR
VVEPGWKRAMPEALAPARGNREAHAPQSLPALQQGQDYAVGEITLKDQQTQPPKPFTEGDLIKAMKNVAKLVDDPRLKQK
LKDTTGIGTEATRAGIIQGLLDRGYLVRQGKALAATPAAFSLIDAVPRPIADPGTTAIWEQALDMVQSGEMPLEEFVAKQ
SAWMSKLVERCAGLRMTISGPPVAAGRNGKPWKKKRSATPRRGSGARTRKPRAAD
>Mature_614_residues
TVTWCIGHLLETAPPDAYDPRYKRWVLADLPIVPERWKMLVKPKTASQFKAVKRLLGECRELVIATDADREGEMIARELV
EHCRYRGPIRRLWLSALDDASIRKALAALKPGAETFNLYHAALGRSRADWLIGMNMSRLFTLLGRQSGYRGVLPVGRVQT
PTLRLVVDRDRSIADFIPVPFWAIDVQLLADGAAFTAQWQAPDDLCDDQGRCLDQARAQQAADAMRNAASARLLKLKTER
QREAAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETHKLITYPRSDCGYLPLSQLGEARAIVAALTQADPTLAALQP
HLDLSRRSRAWNDAKVGAHHGIVPTAAARGLERLAGRPRAVYELIRARYLAQFLPNHEYDRTQADFDCAGQALRAVGKRV
VEPGWKRAMPEALAPARGNREAHAPQSLPALQQGQDYAVGEITLKDQQTQPPKPFTEGDLIKAMKNVAKLVDDPRLKQKL
KDTTGIGTEATRAGIIQGLLDRGYLVRQGKALAATPAAFSLIDAVPRPIADPGTTAIWEQALDMVQSGEMPLEEFVAKQS
AWMSKLVERCAGLRMTISGPPVAAGRNGKPWKKKRSATPRRGSGARTRKPRAAD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=562, Percent_Identity=25.8007117437722, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI10835218, Length=519, Percent_Identity=24.4701348747592, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1788061, Length=616, Percent_Identity=38.3116883116883, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1787529, Length=527, Percent_Identity=27.5142314990512, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32563869, Length=574, Percent_Identity=25.609756097561, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=556, Percent_Identity=25.1798561151079, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=500, Percent_Identity=27.4, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24585251, Length=608, Percent_Identity=25.4934210526316, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640096, Length=549, Percent_Identity=23.8615664845173, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 67763; Mature: 67632

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTWCIGHLLETAPPDAYDPRYKRWVLADLPIVPERWKMLVKPKTASQFKAVKRLLGEC
CCHHHHHHHHHHCCCCCCCCCHHHEEEEECCCCCCHHHHHEECCCCHHHHHHHHHHHHHH
RELVIATDADREGEMIARELVEHCRYRGPIRRLWLSALDDASIRKALAALKPGAETFNLY
HHEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHH
HAALGRSRADWLIGMNMSRLFTLLGRQSGYRGVLPVGRVQTPTLRLVVDRDRSIADFIPV
HHHHCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCC
PFWAIDVQLLADGAAFTAQWQAPDDLCDDQGRCLDQARAQQAADAMRNAASARLLKLKTE
CHHHHHHHHHCCCCCEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQREAAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETHKLITYPRSDCGYLPLSQLG
HHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHC
EARAIVAALTQADPTLAALQPHLDLSRRSRAWNDAKVGAHHGIVPTAAARGLERLAGRPR
HHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
AVYELIRARYLAQFLPNHEYDRTQADFDCAGQALRAVGKRVVEPGWKRAMPEALAPARGN
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCC
REAHAPQSLPALQQGQDYAVGEITLKDQQTQPPKPFTEGDLIKAMKNVAKLVDDPRLKQK
CCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
LKDTTGIGTEATRAGIIQGLLDRGYLVRQGKALAATPAAFSLIDAVPRPIADPGTTAIWE
HHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCEECHHHHHHHHHCCCCCCCCCHHHHHH
QALDMVQSGEMPLEEFVAKQSAWMSKLVERCAGLRMTISGPPVAAGRNGKPWKKKRSATP
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHCCCCC
RRGSGARTRKPRAAD
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVTWCIGHLLETAPPDAYDPRYKRWVLADLPIVPERWKMLVKPKTASQFKAVKRLLGEC
CHHHHHHHHHHCCCCCCCCCHHHEEEEECCCCCCHHHHHEECCCCHHHHHHHHHHHHHH
RELVIATDADREGEMIARELVEHCRYRGPIRRLWLSALDDASIRKALAALKPGAETFNLY
HHEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHH
HAALGRSRADWLIGMNMSRLFTLLGRQSGYRGVLPVGRVQTPTLRLVVDRDRSIADFIPV
HHHHCCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCC
PFWAIDVQLLADGAAFTAQWQAPDDLCDDQGRCLDQARAQQAADAMRNAASARLLKLKTE
CHHHHHHHHHCCCCCEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQREAAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETHKLITYPRSDCGYLPLSQLG
HHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHC
EARAIVAALTQADPTLAALQPHLDLSRRSRAWNDAKVGAHHGIVPTAAARGLERLAGRPR
HHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
AVYELIRARYLAQFLPNHEYDRTQADFDCAGQALRAVGKRVVEPGWKRAMPEALAPARGN
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCC
REAHAPQSLPALQQGQDYAVGEITLKDQQTQPPKPFTEGDLIKAMKNVAKLVDDPRLKQK
CCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
LKDTTGIGTEATRAGIIQGLLDRGYLVRQGKALAATPAAFSLIDAVPRPIADPGTTAIWE
HHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCCEECHHHHHHHHHCCCCCCCCCHHHHHH
QALDMVQSGEMPLEEFVAKQSAWMSKLVERCAGLRMTISGPPVAAGRNGKPWKKKRSATP
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHCCCCC
RRGSGARTRKPRAAD
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]