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The map label for this gene is ywbI [H]

Identifier: 146283199

GI number: 146283199

Start: 3080309

End: 3081268

Strand: Direct

Name: ywbI [H]

Synonym: PST_2864

Alternate gene names: 146283199

Gene position: 3080309-3081268 (Clockwise)

Preceding gene: 146283198

Following gene: 146283200

Centisome position: 67.44

GC content: 68.54

Gene sequence:

>960_bases
TTGGCCCGCAAGCTGCGCATCCACTCGCCGGCCATCCATTATTTCGACATGGTGCGCCGCTGCCAGTCGATCCGCGAAGC
CTCGCGCCGGCTCAACGTTGCCTCCTCGGCGGTCAATCGGCAGATTCTCAAGCTCGAAGACGAGGTCGGCACGCCGCTGT
TCGAGCGACTGCCCGGCGGCCTGCGCCTGACCGCCGCTGGCGAGATCTTCGCCCGCCACGTCATCGTCGTACTGCAGGAC
ACCGAGCGTGTGCGCTCCGAACTGGACGCGCTACAGGGCCTGCGCACCGGCCACGTGGAAATCGCCACGGTATCCGGCGT
CACCACCGATCTGCTGCCCGGCGTGCTGGAACAGCTGCGTGAGCGTTATCCGCGCGTCACGGTCGGCGTAACCAGCCTCG
GCTCGCAGGCCATTCCCGACGCGGTGACCAGCGGCCAGGCGGATATCGGTCTGGCCTTCGCCCTGCCGCGTACCGCCGAC
CTGCAGCAGCTGGGCGTCGGTCATTTCCGTCTGGGCGCCATCGTCGCGCCGGGGCATCCACTGGCCGAACGCCGGGACGT
CAGCTTCGCCACCTGCGCCGAGTACCCGCTGATCCTGGCCAAGAGCGACCTTTCCATCCATCACCTGCTGGCGCCGCTGC
TCGCACGCACTCGCCCGCGGCACAAGGCGCCGCTGGAAACCAGTTCGGTGGAGCTGGCCCTGCAGATGGCCAAGCGCGGC
GCCGGCGTCGCCTTCCAGACACGCATCGGCATCGAGGCCGAGCTGGTCAACGGCACCCTGGTGCACGTTCCGCTGAACGA
CGGCGGCTCGGTATTCAGCGACCTCGGCGTGTACGTGCGCAGTTCGCGTTACCTGCCGGTCGCGGTGGACGCCTTTGTCC
GCGCACTGAGCGAGGAAATCGCCATCCGCGAAGCGGCGCAAGGCACCTCCTCGTCCCGCACAGCGCCGCAGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGGATGACCCGCGCGGACTTCACCCTTGCCAGCGACAACCTGATCGCCATCATCCTCAAGGGCTGCGGGCTCAAGCCACC
GGCAGCGGAGCGCTGAAGGC

Downstream 100 bases:

>100_bases
ACGTTCCGCCCTGGATGCCGCTCTATTGAGCGAACCCACTGCTCAGGAGCGTTCTTGATGGCTTCCCGTCTGCTCGCCGT
ACCGGCTGCCCTGCTTTTCT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MARKLRIHSPAIHYFDMVRRCQSIREASRRLNVASSAVNRQILKLEDEVGTPLFERLPGGLRLTAAGEIFARHVIVVLQD
TERVRSELDALQGLRTGHVEIATVSGVTTDLLPGVLEQLRERYPRVTVGVTSLGSQAIPDAVTSGQADIGLAFALPRTAD
LQQLGVGHFRLGAIVAPGHPLAERRDVSFATCAEYPLILAKSDLSIHHLLAPLLARTRPRHKAPLETSSVELALQMAKRG
AGVAFQTRIGIEAELVNGTLVHVPLNDGGSVFSDLGVYVRSSRYLPVAVDAFVRALSEEIAIREAAQGTSSSRTAPQQR

Sequences:

>Translated_319_residues
MARKLRIHSPAIHYFDMVRRCQSIREASRRLNVASSAVNRQILKLEDEVGTPLFERLPGGLRLTAAGEIFARHVIVVLQD
TERVRSELDALQGLRTGHVEIATVSGVTTDLLPGVLEQLRERYPRVTVGVTSLGSQAIPDAVTSGQADIGLAFALPRTAD
LQQLGVGHFRLGAIVAPGHPLAERRDVSFATCAEYPLILAKSDLSIHHLLAPLLARTRPRHKAPLETSSVELALQMAKRG
AGVAFQTRIGIEAELVNGTLVHVPLNDGGSVFSDLGVYVRSSRYLPVAVDAFVRALSEEIAIREAAQGTSSSRTAPQQR
>Mature_318_residues
ARKLRIHSPAIHYFDMVRRCQSIREASRRLNVASSAVNRQILKLEDEVGTPLFERLPGGLRLTAAGEIFARHVIVVLQDT
ERVRSELDALQGLRTGHVEIATVSGVTTDLLPGVLEQLRERYPRVTVGVTSLGSQAIPDAVTSGQADIGLAFALPRTADL
QQLGVGHFRLGAIVAPGHPLAERRDVSFATCAEYPLILAKSDLSIHHLLAPLLARTRPRHKAPLETSSVELALQMAKRGA
GVAFQTRIGIEAELVNGTLVHVPLNDGGSVFSDLGVYVRSSRYLPVAVDAFVRALSEEIAIREAAQGTSSSRTAPQQR

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787530, Length=182, Percent_Identity=31.8681318681319, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787601, Length=182, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789204, Length=234, Percent_Identity=25.2136752136752, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790783, Length=299, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081904, Length=272, Percent_Identity=24.2647058823529, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082132, Length=281, Percent_Identity=22.4199288256228, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=253, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786401, Length=321, Percent_Identity=25.2336448598131, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=183, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788296, Length=185, Percent_Identity=28.6486486486486, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34542; Mature: 34411

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKLRIHSPAIHYFDMVRRCQSIREASRRLNVASSAVNRQILKLEDEVGTPLFERLPGG
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LRLTAAGEIFARHVIVVLQDTERVRSELDALQGLRTGHVEIATVSGVTTDLLPGVLEQLR
EEEEEHHHHHHCEEEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
ERYPRVTVGVTSLGSQAIPDAVTSGQADIGLAFALPRTADLQQLGVGHFRLGAIVAPGHP
HHCCEEEEEHHHCCCCCCCHHHHCCCCCEEEEEECCCCCCHHHHCCCCEEECEEECCCCC
LAERRDVSFATCAEYPLILAKSDLSIHHLLAPLLARTRPRHKAPLETSSVELALQMAKRG
CCHHCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
AGVAFQTRIGIEAELVNGTLVHVPLNDGGSVFSDLGVYVRSSRYLPVAVDAFVRALSEEI
CCEEEEEECCCEEEEECCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AIREAAQGTSSSRTAPQQR
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ARKLRIHSPAIHYFDMVRRCQSIREASRRLNVASSAVNRQILKLEDEVGTPLFERLPGG
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LRLTAAGEIFARHVIVVLQDTERVRSELDALQGLRTGHVEIATVSGVTTDLLPGVLEQLR
EEEEEHHHHHHCEEEEEEECHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
ERYPRVTVGVTSLGSQAIPDAVTSGQADIGLAFALPRTADLQQLGVGHFRLGAIVAPGHP
HHCCEEEEEHHHCCCCCCCHHHHCCCCCEEEEEECCCCCCHHHHCCCCEEECEEECCCCC
LAERRDVSFATCAEYPLILAKSDLSIHHLLAPLLARTRPRHKAPLETSSVELALQMAKRG
CCHHCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
AGVAFQTRIGIEAELVNGTLVHVPLNDGGSVFSDLGVYVRSSRYLPVAVDAFVRALSEEI
CCEEEEEECCCEEEEECCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AIREAAQGTSSSRTAPQQR
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]