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The map label for this gene is rutR [H]

Identifier: 146283198

GI number: 146283198

Start: 3079771

End: 3080304

Strand: Direct

Name: rutR [H]

Synonym: PST_2863

Alternate gene names: 146283198

Gene position: 3079771-3080304 (Clockwise)

Preceding gene: 146283197

Following gene: 146283199

Centisome position: 67.43

GC content: 63.67

Gene sequence:

>534_bases
ATGAACGCCATCGCCAGCCGCGCCGGGCTGCCCAAGGCCAACGTGCACTATTACTTCAACAGCAAGCTGGGGCTGTACGT
GGAGGTCATGCGGCACATCCTCGAACTCTGGGATACCGCCTTCGACGACCTCACCGTGGACGACGACCCGGCCACCGCCC
TGGCCGAGTACATCCGCATCAAGATGGAATTCTCGCGGCGCCATCCGCAAGCCTCGAAGATCTTCGCCATGGAAGTCATC
AGCGGCTGCGAATGCCTGTCCGAGCACTTCAACCAGGACTATCGCAACTGGTTCCGCGGCCGCGCCGCGGTATTCGACGC
CTGGATCGCCGCCGGCAAGATGGACCCGGTCGACCCCATGCACCTGATCTTCCTGATCTGGGCCGGAACGCAGCACTACG
CCGACTTCTCCGACCAGATCCGCCGCATCAACGGCAAGCGGATGACCCGCGCGGACTTCACCCTTGCCAGCGACAACCTG
ATCGCCATCATCCTCAAGGGCTGCGGGCTCAAGCCACCGGCAGCGGAGCGCTGA

Upstream 100 bases:

>100_bases
CCAAGGCAAGCCTGCCGGCCGCATTCGCCAGAAGAATGAGGAAACCATCCTCGCCGCGGCCGCCGAGGAGTTCGCCCGCT
ACGGTTTCAAGGGCACCAGC

Downstream 100 bases:

>100_bases
AGGCTTGGCCCGCAAGCTGCGCATCCACTCGCCGGCCATCCATTATTTCGACATGGTGCGCCGCTGCCAGTCGATCCGCG
AAGCCTCGCGCCGGCTCAAC

Product: transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MNAIASRAGLPKANVHYYFNSKLGLYVEVMRHILELWDTAFDDLTVDDDPATALAEYIRIKMEFSRRHPQASKIFAMEVI
SGCECLSEHFNQDYRNWFRGRAAVFDAWIAAGKMDPVDPMHLIFLIWAGTQHYADFSDQIRRINGKRMTRADFTLASDNL
IAIILKGCGLKPPAAER

Sequences:

>Translated_177_residues
MNAIASRAGLPKANVHYYFNSKLGLYVEVMRHILELWDTAFDDLTVDDDPATALAEYIRIKMEFSRRHPQASKIFAMEVI
SGCECLSEHFNQDYRNWFRGRAAVFDAWIAAGKMDPVDPMHLIFLIWAGTQHYADFSDQIRRINGKRMTRADFTLASDNL
IAIILKGCGLKPPAAER
>Mature_177_residues
MNAIASRAGLPKANVHYYFNSKLGLYVEVMRHILELWDTAFDDLTVDDDPATALAEYIRIKMEFSRRHPQASKIFAMEVI
SGCECLSEHFNQDYRNWFRGRAAVFDAWIAAGKMDPVDPMHLIFLIWAGTQHYADFSDQIRRINGKRMTRADFTLASDNL
IAIILKGCGLKPPAAER

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=169, Percent_Identity=39.6449704142012, Blast_Score=145, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 20198; Mature: 20198

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIASRAGLPKANVHYYFNSKLGLYVEVMRHILELWDTAFDDLTVDDDPATALAEYIRI
CCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KMEFSRRHPQASKIFAMEVISGCECLSEHFNQDYRNWFRGRAAVFDAWIAAGKMDPVDPM
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
HLIFLIWAGTQHYADFSDQIRRINGKRMTRADFTLASDNLIAIILKGCGLKPPAAER
HHHHHHCCCCCHHCCHHHHHHHHCCCEEECCCCEECCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNAIASRAGLPKANVHYYFNSKLGLYVEVMRHILELWDTAFDDLTVDDDPATALAEYIRI
CCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KMEFSRRHPQASKIFAMEVISGCECLSEHFNQDYRNWFRGRAAVFDAWIAAGKMDPVDPM
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
HLIFLIWAGTQHYADFSDQIRRINGKRMTRADFTLASDNLIAIILKGCGLKPPAAER
HHHHHHCCCCCHHCCHHHHHHHHCCCEEECCCCEECCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]